标签： Oligonucleotide synthesis

dU (Deoxyuridine) phosphoramidite

dU (Deoxyuridine) phosphoramidite,lumiprobe代理,lumiprobe产品

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  • dU (Deoxyuridine) phosphoramidite

dU (Deoxyuridine) phosphoramidite

标签：Oligonucleotide synthesis,Phosphoramidite
货号 规格 价格 货期
320-1g 1 g – 10 days
320-10g 10 g 250.00$ 21 days
320-50g 50 g please inquire 21 days

dU (Deoxyuridine) phosphoramidite相关产品

FAM-dT phosphoramidite

Phosphoramidite for synthesis of oligonucleotides labeled with fluorescein (FAM) in the middle of sequence.

亚磷酰胺用于合成在序列中间用荧光素 (FAM) 标记的寡核苷酸。

Alkyne dT phosphoramidite

Phosphoramidite modifier for the synthesis of oligonucleotides containing alkyne modification in the middle of the chain.

亚磷酰胺修饰剂，用于合成在链中间含有炔烃修饰的寡核苷酸。

Hexaethylene glycol phosphoramidite

Spacer phosphoramidite 18 (hexaethylene glycol amidite) provides a long hydrophilic linker for oligo synthesis.

间隔亚磷酰胺 18（六甘醇酰胺）为寡核苷酸合成提供了一个长的亲水接头。

General properties

Appearance: pale yellow solid foam
Molecular weight: 730.79
CAS number: 109389-30-2
Molecular formula: C39H47N4O8P
IUPAC name: 5′-O-(4,4′-Dimethoxytrityl)-2′-deoxyuridine-3′-O-[O-(2-cyanoethyl)-N,N’-diisopropylphosphoramidite]
Solubility: good in acetonitrile, DCM
Quality control: NMR 1H, NMR 31P, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 干燥。
MSDS: Download
Product specifications

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases

TFA-Amino modifier CPG 500

TFA-Amino modifier CPG 500,这种高负载、500 Å 可控孔玻璃固体支持物设计用于合成含有多达 50 个具有 3′-氨基的碱基的寡核苷酸。 氨基受到三氟乙酰 (TFA) 保护，在标准去封闭条件下可轻松去除。

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  • TFA-Amino modifier CPG 500

TFA-Amino modifier CPG 500

标签：Oligonucleotide synthesis,CPG (controlled pore glass) solid supports for oligonucleotide synthesis
货号 规格 价格 货期
11860 100 mg 60.00$ in stock
21860 250 mg 120.00$ in stock
31860 500 mg 230.00$ in stock
41860 1 g 380.00$ in stock
51860 5 g 1450.00$ 21 days
61860 10 g 2465.00$ 21 days

This high load, 500 Å controlled pore glass solid support is designed for the synthesis of oligonucleotides containing up to 50 bases with a 3′-amino group. Amino group is protected with trifluoroacetyl (TFA) protection which is easily removed under standard deblock conditions.

The reagent is based on hydroxyprolinol core – a universal non-nucleoside structure that is naturally 100% chiral (no isomers formed upon condensation), and stable to all conditions of oligonucleotide synthesis and deblock.

这种高负载、500 Å 可控孔玻璃固体支持物设计用于合成含有多达 50 个具有 3′-氨基的碱基的寡核苷酸。 氨基受到三氟乙酰 (TFA) 保护，在标准去封闭条件下可轻松去除。

该试剂基于羟基脯氨酸核心 – 一种通用的非核苷结构，天然 100% 手性（缩合时不形成异构体），并且对所有寡核苷酸合成和去封闭条件都稳定。

TFA-Amino modifier CPG 500 TFA-Amino modifier CPG 500 TFA-Amino modifier CPG 500 TFA-Amino modifier CPG 500

TFA-Amino modifier CPG 500相关产品

DMS(O)MT aminolink C6

An amidite for the introduction of amino group.

用于引入氨基的亚酰胺。

FAM Phosphoramidite, 6-Isomer

Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.

用于合成修饰寡核苷酸的单一异构体 6-FAM 亚磷酰胺。

DusQ2 dT phosphoramidite

General properties

Appearance: off-white beads
Quality control: NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement
Storage conditions: Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50−80
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases

相关文献：

  1. Taskova, M.; Uhd, J.; Miotke, L.; Kubit, M.; Bell, J.; Ji, H.P.; Astakhova, K. Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence. Analytical Chemistry, 2017, 89(8), 4363–4366. doi: 10.1021/acs.analchem.7b00646

FAM CPG 500, 6-isomer

FAM CPG 500, 6-isomer,可控孔玻璃固体支持物用于合成 3′ 标记的寡核苷酸。 该固体支持物用于合成在寡核苷酸的 3′-末端带有荧光素 (FAM) 荧光染料的寡核苷酸。 它含有纯6-异构体的荧光素。 该试剂的结构基于手性、对映体纯的羟基脯氨酸支架。

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  • FAM CPG 500, 6-isomer

FAM CPG 500, 6-isomer

标签：FAM (Fluorescein), Oligonucleotide synthesis, CPG (controlled pore glass) solid supports for oligonucleotide synthesis
货号 规格 价格 货期
15760 100 mg 95.00$ in stock
35760 500 mg 295.00$ in stock
45760 1 g 469.00$ in stock
55760 5 g please inquire in stock
Controlled pore glass solid supports are used for the synthesis of 3′-labeled oligonucleotides. This solid support is intended for the synthesis of oligonucleotides bearing fluorescein (FAM) fluorescent dye on 3′-terminus of oligonucleotide. It contains pure 6-isomer of fluorescein. The structure of the reagent is based on chiral, enantiomerically pure scaffold of hydroxyprolinol.

The solid support ensures optimal yield of oligonucleotides up to 60mer. For longer oligos, CPG 1000 should be used. The reagent is compatible with standard ammonia cleavage and deblocking conditions.

可控孔玻璃固体支持物用于合成 3′ 标记的寡核苷酸。 该固体支持物用于合成在寡核苷酸的 3′-末端带有荧光素 (FAM) 荧光染料的寡核苷酸。 它含有纯6-异构体的荧光素。 该试剂的结构基于手性、对映体纯的羟基脯氨酸支架。

固体支持物确保寡核苷酸的最佳产量高达 60 聚体。 对于较长的寡核苷酸，应使用 CPG 1000。 该试剂与标准氨裂解和解封条件兼容。

FAM CPG 500, 6-isomer FAM CPG 500, 6-isomer FAM CPG 500, 6-isomer FAM CPG 500, 6-isomer

FAM CPG 500, 6-isomer

Cyanine7 NHS ester

Amine reactive NHS ester of near infrared dye Cyanine7.

近红外染料Cy7的胺活性NHS酯。

Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

Catalyst for bioconjugate synthesis by Click Chemistry.

Click Chemistry 用于生物共轭合成的催化剂。

Phosphate CPG 500 solid support

CPG solid support for the synthesis of 3′-phosphorylated oligonucleotides.

用于合成 3′-磷酸化寡核苷酸的 CPG 固体支持物。

General properties

Appearance: off white beads
MSDS: Download
Product specifications

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 65±15
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases

Алкин-ПЭГ4-фосфорамидит

Алкин-ПЭГ4-фосфорамидит,包含在亚磷酰胺结构中的四甘醇接头提供了增加的亲水性，并且还降低了合成缀合物的毒性和免疫原性。

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  • Алкин-ПЭГ4-фосфорамидит
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Алкин-ПЭГ4-фосфорамидит

标签：Alkynes,Oligonucleotide synthesis,Phosphoramidite,PEG4 (tetraethylene glycol)
Cat. # Quantity Price Lead time
2779-100mg 100 mg 390.00$ 21 days
2779-250mg 250 mg 680.00$ 21 days
2779-1g 1 g 1640.00$ 21 days
2779-5g 5 g please inquire 21 days

 

Алкин-ПЭГ4-фосфорамидит — представляет собой фосфорамидит на основе тетраэтиленгликоля с терминальной тройной связью и может использоваться для синтеза различных функционализированных олигонуклеотидов. Алкиновый фрагмент в синтезированных молекулах вступает в реакции Click Chemistry с соединениями, содержащими азидную группу в присутствии солей меди(I), приводя к различным олигонуклеотидным конъюгатам, например с красителями, пептидами или гликанами. Выход полученных подобным образом соединений довольно высок, а выделение и очистка продуктов зачастую проще по сравнению с молекулами, полученные в результате взаимодействия амино-модифицированных олигонуклеотидов с активированными эфирами, например, красителей. Важной характеристикой Click Chemistry реакций является их биоортогональность, поскольку ни терминальные алкиновые, ни азидные функциональные группы обычно не присутствуют в биомолекулах.

Тетраэтиленгликольный линкер, входящий в структуру фосфорамидита, обеспечивает повышенную гидрофильность, а также снижает токсичность и иммуногенность синтезированных конъюгатов.

炔烃-PEG4-亚磷酰胺是一种基于四甘醇的亚磷酰胺，具有末端三键，可用于合成各种功能化的寡核苷酸。合成分子中的炔烃片段在铜 (I) 盐存在下与含有叠氮基的化合物进入 Click Chemistry 反应，从而产生各种寡核苷酸缀合物，例如与染料、肽或聚糖。以这种方式获得的化合物的产率非常高，与由氨基修饰的寡核苷酸与活化的酯（例如染料）相互作用获得的分子相比，产物的分离和纯化通常更简单。 Click Chemistry 反应的一个重要特征是它们的生物正交性，因为末端炔烃和叠氮官能团通常不存在于生物分子中。

包含在亚磷酰胺结构中的四甘醇接头提供了增加的亲水性，并且还降低了合成缀合物的毒性和免疫原性。

Общие свойства

Вид продукта: желтое масло
Молекулярная масса: 432.49
CAS-номер: 1682657-14-2
Брутто-формула: C20H37N2O6P
Название IUPAC: 2-cyanoethyl (3,6,9,12-tetraoxapentadec-14-yn-1-yl) diisopropylphosphoramidite
Растворимость: хорошая в ацетонитриле и дихлорметане
Контроль качества: ЯМР 1H и 31P, ВЭЖХ-МС (95%)
Условия хранения: Хранение: 12 месяцев (с момента доставки) при -20°C в темноте. Транспортировка: до трех недель при комнатной температуре. Берегите от влаги.
MSDS: Download
Product specifications

Олигонуклеотидный синтез

Растворитель: сухой ацетонитрил (растворить до концентрации 0.1 М, хранение – 1 неделя).
Условия конденсации: Стандартное, как для фосфорамидитов природных нуклеозидов.
Условия деблокирования: идентичные защищенным природным нуклеотидам

Алкин-ПЭГ4-фосфорамидит

TET phosphoramidite, 6-isomer

TET phosphoramidite, 6-isomer,TET 亚磷酰胺用于合成荧光标记的寡核苷酸，纯 6 异构体。

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TET phosphoramidite, 6-isomer

标签：Oligonucleotide synthesis,Phosphoramidite,TET
货号 规格 价格 货期
C8160 100 mg – 10 days
D8160 250 mg 340.00$ in stock
F8160 1 g 990.00$ in stock
G8160 5 g 3900.00$ 10 days
H8160 10 g please inquire 10 days

TET phosphoramidite for synthesis of fluorescently labeled oligonucleotides, pure 6-isomer.

TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate (absorption maximum at 519 nm, emission maximum at 535 nm).

TET phosphoramidite is used for synthesis of fluorescently-labeled primers and hybridization probes for qPCR. TET can be used with DusQ1 fluorescence quencher (can be used with 500 Å DusQ1 CPG 500).

5’-labeled primers are used with non-labeled reverse primers for microsatellite amplification via RCR followed by fragment analysis. TET-labeled amplification products can be analyzed using various sequencers for capillary electrophoresis, including ABI PRISM® 310 Genetic Analyzer.

TET 亚磷酰胺用于合成荧光标记的寡核苷酸，纯 6 异构体。

TET（四氯荧光素）是一种绿色荧光荧光素衍生物（最大吸收波长为 519 nm，最大发射波长为 535 nm）。

TET 亚磷酰胺用于合成荧光标记的引物和用于 qPCR 的杂交探针。 TET 可与 DusQ1 荧光猝灭剂一起使用（可与 500 Å DusQ1 CPG 500 一起使用）。

5′ 标记的引物与未标记的反向引物一起使用，通过 RCR 进行微卫星扩增，然后进行片段分析。 TET 标记的扩增产物可以使用各种毛细管电泳测序仪进行分析，包括 ABI PRISM® 310 遗传分析仪。

Recommendations for using the reagent:

使用试剂的建议：

Condensation: 3 min.

Deprotection: standard conditions with 25% aqueous ammonium solution; deprotection time depends on nucleic base profile and their protective properties (deprotection for 17 hours at 55°C removes all protection groups from standard nucleic acids). AMA (1:1 mixture of concentrated aqueous ammonium/40% aqueous methylamine) can be used with ~5% non-fluorescent by-product forming. To avoid formation of the by-product, start deprotection with ammonium (30 min at room temperature), then add an equivalent volume of 40% aqueous methylamine solution and continue deprotection as usual with AMA (10 min at 65°C).

冷凝：3 分钟。

脱保护：标准条件用25%铵水溶液； 脱保护时间取决于核酸碱基谱及其保护特性（在 55°C 脱保护 17 小时会去除标准核酸中的所有保护基团）。 AMA（浓铵水溶液/40% 甲胺水溶液的 1:1 混合物）可用于形成约 5% 的非荧光副产物。 为避免形成副产物，用铵开始脱保护（室温下 30 分钟），然后加入等量体积的 40% 甲胺水溶液，并像往常一样用 AMA 继续脱保护（65°C 下 10 分钟）。

TET phosphoramidite, 6-isomer TET phosphoramidite, 6-isomer TET phosphoramidite, 6-isomer

Absorption and emission spectra of TET

TET phosphoramidite, 6-isomer

TET phosphoramidite, 6-isomer相关产品

JOE phosphoramidite, 6-isomer

JOE dye phosphoramidite for synthesis of labeled oligonucleotides. JOE absorption and emission maxima are between maxima of FAM and TAMRA/ROX dyes.

JOE 染料亚磷酰胺用于合成标记的寡核苷酸。 JOE 吸收和发射最大值介于 FAM 和 TAMRA/ROX 染料的最大值之间。

HEX phosphoramidite, 6-isomer

HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.

用于合成 5′ 标记寡核苷酸的 HEX 亚磷酰胺。

General properties

Appearance: white solid foam
Molecular weight: 981.72
CAS number: 877049-90-6
Molecular formula: C46H54N3Cl4O10P
IUPAC name: 2′,4,7,7′-tetrachloro-6-((6-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl bis(2,2-dimethylpropanoate)
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and 31P, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 519
ε, L⋅mol−1⋅cm−1: 100000
Emission maximum, nm: 535
Fluorescence quantum yield: 0.47
CF260: 0.17
CF280: 0.09

Oligo synthesis details寡核苷酸合成细节

Diluent:

冲淡：

 anhydrous acetonitrile (prepare a 0.1 М solution, storage 1 week).

无水乙腈（制备 0.1 М 溶液，储存 1 周）。

R6G phosphoramidite, 6-isomer

R6G phosphoramidite, 6-isomer,用于寡核苷酸合成的 R6G 亚磷酰胺，纯 6 异构体。

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R6G phosphoramidite, 6-isomer

标签：Oligonucleotide synthesis,Phosphoramidite,R6G
货号 规格 价格 货期
C5260 100 mg – in stock
D5260 250 mg 190.00$ in stock
F5260 1 g 590.00$ in stock
G5260 5 g 2390.00$ in stock
H5260 10 g please inquire 10 days

R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer.

R6G (rhodamine 6G) is a xanthene dye of the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to those of fluorescein, the absorption (518 nm) and emission (542 nm) maxima of 6-R6G are shifted into the long-wave region.

R6G can be used as a FRET acceptor in DNA-sequencing and in short tandem repeat (STR) analysis. The dye is commonly used for producing TaqMan probes for quantitative PCR. In comparison with HEX and JOE dyes, R6G in combination with BHQ1 quencher has stronger quenching in molecular beacon probes, significantly reducing background fluorescence in PCR.

用于寡核苷酸合成的 R6G 亚磷酰胺，纯 6 异构体。

R6G（罗丹明 6G）是罗丹明家族的一种呫吨染料，具有高荧光量子产率和高摩尔消光系数。 相对于荧光素，6-R6G 的最大吸收 (518 nm) 和发射 (542 nm) 转移到长波区域。

R6G 可用作 DNA 测序和短串联重复 (STR) 分析中的 FRET 受体。 该染料通常用于生产用于定量 PCR 的 TaqMan 探针。 与 HEX 和 JOE 染料相比，R6G 与 BHQ1 猝灭剂组合在分子信标探针中具有更强的猝灭作用，显着降低了 PCR 中的背景荧光。

Usage:

Coupling: 10 minutes.

Deprotection: solution of tert-Butylamine in water (1:3 v/v) overnight at 55°С.

Aqueous ammonia and AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) must NOT be used for deprotection of the modified oligonucleotide from the solid-phase support because of complete and irreversible R6G degradation.

耦合：10 分钟。

脱保护：叔丁胺水溶液 (1:3 v/v) 在 55°С 下过夜。

由于 R6G 完全且不可逆地降解，因此不得使用氨水和 AMA（30% 氢氧化铵/40% 甲胺水溶液 1:1 v/v）将修饰的寡核苷酸从固相载体上脱保护。

R6G phosphoramidite, 6-isomer R6G phosphoramidite, 6-isomer R6G phosphoramidite, 6-isomer

Absorption and emission spectra of R6G, 6-isomer

R6G phosphoramidite, 6-isomer

R6G phosphoramidite, 6-isomer相关产品

ROX azide, 5- isomer

ROX (aka rhodamine X or rhodamine 101) azide for biomolecule labeling through Click Chemistry.

ROX（又名罗丹明 X 或罗丹明 101）叠氮化物，用于通过 Click Chemistry 进行生物分子标记。

Cyanine3 azide

Azide derivative of Cyanine3 fluorescent dye for Click Chemistry.

用于 Click Chemistry 的 Cyanine3 荧光染料的叠氮化物衍生物。

ROX NHS ester, 6- isomer

Pure isomer 6-ROX NHS ester for amine labeling.

用于胺标记的纯异构体 6-ROX NHS 酯。

General properties

Appearance: Yellowish foam
Molecular weight: 949.93
CAS number: 1355330-47-0
Molecular formula: C46H54F6N5O8P
Solubility: good in DCM, acetonitrile
Quality control: NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下最多保存 2 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 518
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 542
Fluorescence quantum yield: 0.95
CF260: 0.18
CF280: 0.17

Oligo synthesis details

Diluent: acetonitrile

Pyrene phosphoramidite dU

Pyrene phosphoramidite dU,Pyrene 是一种多环芳烃，以其嵌入 DNA 的能力而闻名。 芘具有强烈的蓝色荧光。 其参数很大程度上取决于荧光团微环境。 因此，芘的荧光光谱用于提取芘周围位点的结构信息。 两个非常接近的芘通常形成很容易被准分子荧光检测到的准分子。 芘也可以是其他荧光团（如苝）的 FRET 供体。

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Pyrene phosphoramidite dU

Oligonucleotide synthesis,Phosphoramidite,Pyrene
货号 规格 价格 货期
11460 100 mg 150.00$ in stock
21460 250 mg 320.00$ in stock
41460 1 g 880.00$ in stock
61460 5 g please inquire in stock
Pyrene is a polycyclic aromatic hydrocarbon that is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on the fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about the site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.

With this phosphoramidite, pyrene can be introduced into DNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows attaching pyrene fragment to 5′, internal, or 3′ position (using universal support).

This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.

Pyrene 是一种多环芳烃，以其嵌入 DNA 的能力而闻名。 芘具有强烈的蓝色荧光。 其参数很大程度上取决于荧光团微环境。 因此，芘的荧光光谱用于提取芘周围位点的结构信息。 两个非常接近的芘通常形成很容易被准分子荧光检测到的准分子。 芘也可以是其他荧光团（如苝）的 FRET 供体。

使用这种亚磷酰胺，可以通过自动合成将芘引入 DNA。 这种芘亚磷酰胺包含刚性连接到脱氧尿苷的烃部分。 该试剂允许将芘片段连接到 5’、内部或 3′ 位置（使用通用支架）。

这种亚酰胺不需要特殊的处理、偶联或脱保护条件。 推荐的稀释剂是乙腈。

Pyrene phosphoramidite dU Pyrene phosphoramidite dU Pyrene phosphoramidite dU Pyrene phosphoramidite dU

Absorption and emission spectra of pyrene fluorophore

Pyrene phosphoramidite dU

Pyrene phosphoramidite dU相关产品

Pyrenebutyric acid NHS ester

Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.

芘 NHS 酯，用于用芘残基标记生物分子。 芘是一种形成准分子的荧光邻近探针，也是石墨固定的锚定基团。

Sulfo-Cyanine5 maleimide

Thiol reactive, water soluble cyanine dye for Cy5® channel.

用于 Cy5® 的硫醇活性水溶性花青染料 渠道。

Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

胺反应性 Cy5 活化酯，用于标记蛋白质、肽和其他分子。

General properties

Appearance: yellowish foam
Molecular weight: 955.04
CAS number: 199920-17-7
Molecular formula: C57H55N4O8P
Solubility: good in dichloromethane and acetonitrile

良好的二氯甲烷和乙腈
Quality control: NMR 1H (95%), 31P, HPLC-MS
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 260; 282; 365; 392
ε, L⋅mol−1⋅cm−1: 12600; 21900; 16000; 14200
Emission maximum, nm: 460
Fluorescence quantum yield: ~0.1

Oligo synthesis details

Diluent: acetonitrile

Perylene dU phosphoramidite

Perylene dU phosphoramidite,Oligonucleotide synthesis,Phosphoramidite，Perylene 是一种明亮且极耐光的荧光多环芳烃 (PAH) 标记，其量子产率接近定量。 由于荧光的低寿命，该探针不会形成准分子。,

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  • Perylene dU phosphoramidite

Perylene dU phosphoramidite

标签：Oligonucleotide synthesis,Phosphoramidite
货号 规格 价格 货期
14460 100 mg 150.00$ in stock
24460 250 mg 320.00$ in stock
44460 1 g 880.00$ in stock
64460 5 g please inquire 10 days

Perylene is a bright and extremely photostable fluorescent polycyclic aromatic hydrocarbon (PAH) label with a quantum yield approaching quantitative. Due to the low lifetime of fluorescence, this probe does not form excimers.

With this phosphoramidite, perylene can be introduced into DNA by automated oligonucleotide synthesis. Perylene is attached to the 5′ position of deoxyuridine (dU) through a triple bond and the fluorophore is electronically coupled to the deoxyuridine base. This coupling of dU and perylene makes the fluorescence sensitive to the base pairing of the dU portion of the molecule, allowing the discrimination between perfect and one base mismatched targets.

This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent is acetonitrile.

Perylene 是一种明亮且极耐光的荧光多环芳烃 (PAH) 标记，其量子产率接近定量。 由于荧光的低寿命，该探针不会形成准分子。

使用这种亚磷酰胺，可以通过自动寡核苷酸合成将苝引入 DNA。 苝通过三键连接到脱氧尿苷 (dU) 的 5′ 位置，并且荧光团与脱氧尿苷碱基电子耦合。 dU 和苝的这种偶联使荧光对分子的 dU 部分的碱基配对敏感，从而可以区分完美和一个碱基错配的靶标。

这种亚酰胺不需要特殊的处理、偶联或脱保护条件。 推荐的稀释剂是乙腈。

Perylene dU phosphoramidite相关产品

HEX phosphoramidite, 6-isomer

HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.

用于合成 5′ 标记寡核苷酸的 HEX 亚磷酰胺。

Hexaethylene glycol phosphoramidite

Spacer phosphoramidite 18 (hexaethylene glycol amidite) provides a long hydrophilic linker for oligo synthesis.

间隔亚磷酰胺 18（六甘醇酰胺）为寡核苷酸合成提供了一个长的亲水接头。

Biotin phosphoramidite (hydroxyprolinol)

Biotin phosphoramidite reagent for the synthesis of biotinylated oligos. Contains a dimethyoxytrityl group.

用于合成生物素化寡核苷酸的生物素亚磷酰胺试剂。 含有二甲氧基三苯甲基。

General properties

Appearance: orange solid
Molecular weight: 1005.1
CAS number: 908117-78-2
Molecular formula: C61H57N4O8P
Solubility: good in dichloromethane and acetonitrile
Quality control: NMR 1H, 31P, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 435; 408; 252
ε, L⋅mol−1⋅cm−1: 36000
Emission maximum, nm: 439; 467
Fluorescence quantum yield: 1.0

Oligo synthesis details

Diluent: acetonitrile

JOE phosphoramidite, 6-isomer

JOE phosphoramidite, 6-isomer,用于寡核苷酸合成的 JOE 亚磷酰胺，纯 6-异构体 (6-JOE)。

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JOE phosphoramidite, 6-isomer

标签：Oligonucleotide synthesis, Phosphoramidite, JOE
货号 规格 价格 货期
12160 100 mg – in stock
D2160 250 mg 170.00$ 10 days
F2160 1 g 590.00$ in stock
G2160 5 g 2290.00$ in stock
H2160 10 g 3390.00$ in stock
K2160 20 g please inquire 10 days

JOE phosphoramidite for oligonucleotide synthesis, pure 6-isomer (6-JOE).

Fluorescent dye JOE is a fluorescein derivative containing two chlorine atoms and two methoxy groups. Its absorption and emission maxima are at 503 nm and 525 nm, respectively. By its spectral characteristics JOE is found in between FAM and TAMRA/ROX; therefore, this fluorophor is commonly used for multiplex detection, including that during DNA sequencing.

Our catalog also contains JOE phosphoramidite, 5-isomer. We compared qPCR probes containing different JOE isomers (5-JOE and 6-JOE) and did not find any significant differences between them.

用于寡核苷酸合成的 JOE 亚磷酰胺，纯 6-异构体 (6-JOE)。

荧光染料 JOE 是一种含有两个氯原子和两个甲氧基的荧光素衍生物。 其吸收和发射最大值分别为 503 nm 和 525 nm。 根据其光谱特性，JOE 介于 FAM 和 TAMRA/ROX 之间； 因此，这种荧光团通常用于多重检测，包括 DNA 测序期间的检测。

我们的目录还包含 JOE 亚磷酰胺，5 异构体。 我们比较了含有不同 JOE 异构体（5-JOE 和 6-JOE）的 qPCR 探针，并没有发现它们之间有任何显着差异。

Usage

Coupling: 6 minutes

Deprotection: standard conditions using ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °С removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C).

耦合：6 分钟

脱保护：使用氢氧化铵的标准条件； 脱保护时间取决于寡核苷酸组成和核碱基保护基团（在 55°С 脱保护 17 小时会去除标准核碱基上的所有保护基团）。 AMA（30% 氢氧化铵/40% 甲胺水溶液 1:1 v/v 的溶液）可用于形成约 5% 的非荧光副产物。 为避免形成副产物，用氢氧化铵开始脱保护（室温下 30 分钟），然后加入等体积的 40% 甲胺水溶液，并根据需要使用 AMA 继续脱保护（例如，在 65°C 下 10 分钟）。

JOE phosphoramidite, 6-isomer JOE phosphoramidite, 6-isomer JOE phosphoramidite, 6-isomer JOE phosphoramidite, 6-isomer

Absorption and emission spectra of JOE

JOE phosphoramidite, 6-isomer

JOE phosphoramidite, 6-isomer相关产品

ROX NHS ester, 6- isomer

Pure isomer 6-ROX NHS ester for amine labeling.

用于胺标记的纯异构体 6-ROX NHS 酯。

Cyanine5.5 NHS ester

Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.

Cy5.5 NHS 是远红/近红外胺活性染料。

BDP R6G azide

BDP R6G is a high performance borondipyrromethene fluorophore which is tuned to match excitation and emission channels of R6G dye.

BDP R6G 是一种高性能硼二吡咯亚甲基荧光团，经过调整以匹配 R6G 染料的激发和发射通道。

General properties

Appearance: white solid
Molecular weight: 972.88
Molecular formula: C48H60N3Cl2O12P
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and 31P, HPLC-MS
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 554
Fluorescence quantum yield: 0.61
CF260: 0.36
CF280: 0.28

Oligo synthesis details

Diluent: acetonitrile

JOE phosphoramidite, 5-isomer

Oligonucleotide synthesis, Phosphoramidite, JOE,JOE 是一种呫吨染料，具有两个氯和两个甲氧基取代基的荧光素衍生物。 这种荧光团是寡核苷酸的有用标记。 其吸收和发射光谱位于 FAM 和 TAMRA 之间。

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JOE phosphoramidite, 5-isomer

标签：Oligonucleotide synthesis, Phosphoramidite ,JOE
货号 规格 价格 货期
C1160 100 mg – 5 days
D1160 250 mg 170.00$ 5 days
F1160 1 g 590.00$ in stock
G1160 5 g 2290.00$ in stock
H1160 10 g 3390.00$ in stock

JOE is a xanthene dye, fluorescein derivative possessing two chloro and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.

The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains pure isomer of 5-JOE dye.

JOE 是一种呫吨染料，具有两个氯和两个甲氧基取代基的荧光素衍生物。 这种荧光团是寡核苷酸的有用标记。 其吸收和发射光谱位于 FAM 和 TAMRA 之间。

可以使用这种亚磷酰胺将荧光团引入寡核苷酸中。 它可以承受标准的铵解封条件。 本产品含有5-JOE染料的纯异构体。

JOE phosphoramidite, 5-isomer JOE phosphoramidite, 5-isomer JOE phosphoramidite, 5-isomer

Absorption and emission spectra of JOE dye

JOE phosphoramidite, 5-isomer

Oligonucleotide synthesis, Phosphoramidite, JOE相关产品

dsGreen for Real-Time PCR, 100×

Stock solution of dsGreen for Real-Time PCR, 100×

用于实时 PCR 的 dsGreen 储备溶液，100×

Sulfo-Cyanine5.5 azide

Sulfo-Cyanine5.5 azide is a derivative of water soluble far red emitting fluorescent cyanine dye. The azide group can be conjugated by either copper-catalyzed or copper free Click chemistry. The reagent possesses high aqueous solubility and hydrophilicity, and recommended for the labeling of biomolecules in aqueous environment.

Sulfo-Cy5.5 azide 是水溶性远红荧光花青染料的衍生物。 叠氮基可以通过铜催化或无铜点击化学进行缀合。 该试剂具有高水溶性和亲水性，推荐用于水环境中生物分子的标记。

Sulfo-Cyanine5.5 amine

Sulfo-Cyanine5.5 is a far red/NIR fluorophore possessing high hydrophilicity and aqueous solubility. The dye displays good brightness in far red region due to outstanding molar extinction coefficient. This is amine derivative which is reactive towards electrophiles. It can also be used for enzymatic transamination labeling.

Sulfo-Cy5.5 是一种远红/近红外荧光团，具有高亲水性和水溶性。 由于出色的摩尔消光系数，该染料在远红色区域显示出良好的亮度。 这是对亲电试剂有反应性的胺衍生物。 它也可用于酶促转氨标记。

General properties

Appearance: colorless solid
Molecular weight: 972.88
Molecular formula: C48H60N3Cl2O12P
Solubility: good in DCM, acetonitrile
Quality control: NMR 1H, 31P, HPLC-MS (95%), functional testing
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 554
Fluorescence quantum yield: 0.61
CF260: 0.36
CF280: 0.28

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases

Inosine (dI) phosphoramidite

Inosine (dI) phosphoramidite,脱氧肌苷是在天然核酸中遇到的次要碱基。 它与 A、C 和 T/U 碱基形成碱基对。 使用这种亚磷酰胺可以合成含有 2′-脱氧肌苷的修饰 DNA 寡核苷酸。

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  • Inosine (dI) phosphoramidite

Inosine (dI) phosphoramidite

标签：Oligonucleotide synthesis,Phosphoramidite
货号 规格 价格 货期
319-1g 1 g 190.00$ in stock
319-5g 5 g 750.00$ in stock

Deoxyinosine is a minor base that is encountered in natural nucleic acids. It forms base pairs with A, C and T/U bases. The modified DNA oligonucleotides containing 2′-deoxyinosine can be synthesized using this phosphoramidite.

脱氧肌苷是在天然核酸中遇到的次要碱基。 它与 A、C 和 T/U 碱基形成碱基对。 使用这种亚磷酰胺可以合成含有 2′-脱氧肌苷的修饰 DNA 寡核苷酸。

Inosine (dI) phosphoramidite Inosine (dI) phosphoramidite Inosine (dI) phosphoramidite Inosine (dI) phosphoramidite

Inosine (dI) phosphoramidite相关产品

Hexaethylene glycol phosphoramidite

Spacer phosphoramidite 18 (hexaethylene glycol amidite) provides a long hydrophilic linker for oligo synthesis.

间隔亚磷酰胺 18（六甘醇酰胺）为寡核苷酸合成提供了一个长的亲水接头。

Biotin phosphoramidite (hydroxyprolinol)

Biotin phosphoramidite reagent for the synthesis of biotinylated oligos. Contains a dimethyoxytrityl group.

用于合成生物素化寡核苷酸的生物素亚磷酰胺试剂。 含有二甲氧基三苯甲基。

R6G phosphoramidite, 6-isomer

Phosphoramidite for synthesis of oligonucleotides labeled at the 5′-terminus with rhodamine 6G (R6G). Pure 6-isomer.

亚磷酰胺用于合成在 5′ 末端用罗丹明 6G (R6G) 标记的寡核苷酸。 纯 6 异构体。

General properties

Appearance: white solid
Molecular weight: 754.81
CAS number: 141684-35-7
Molecular formula: C40H47N6O7P
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and 31P, HPLC-MS
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Deprotection conditions: identical to protected nucleobases

HEX phosphoramidite, 6-isomer

HEX phosphoramidite, 6-isomer,HEX（六氯荧光素）是一种荧光素衍生物，在黄色光谱范围内发射（最大吸收在 533 nm，最大发射在 549 nm）。

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  • HEX phosphoramidite, 6-isomer

HEX phosphoramidite, 6-isomer

标签：Oligonucleotide synthesis, Phosphoramidite ,HEX (Hexachlorofluorescein)
货号 规格 价格 货期
CB260 100 mg – 10 days
DB260 250 mg 360.00$ in stock
FB260 1 g 760.00$ in stock
GB260 5 g 3490.00$ 21 days
HB260 10 g please inquire 21 days

HEX phosphoramidite for oligonucleotide synthesis, pure 6-isomer.

HEX (hexachlorofluorescein) is a fluorescein derivative with emission in the yellow spectrum range (absorption maximum at 533 nm, emission maximum at 549 nm).

HEX phosphoramidite is used for synthesis of fluorescent-labeled primers and hybridization probes such as TaqMan, Molecular Beacon, and Scorpion for qPCR. HEX is most effectively quenched by non-fluorescent DusQ1 dark quencher because of significant overlapping of their spectra (convenient for use with DusQ1 CPG 500 solid support with a pore size of 500 Å). Many automated sequencers based on capillary gel electrophoresis have a detection channel for HEX. Therefore, this phosphoramidite is commonly used for synthesis of 5’-labeled oligonucleotides for fragment analysis, particularly for microsatellite analysis, when microsatellite loci are amplified using a fluorescent-labeled forward primer and a non-labeled reverse primer.

用于寡核苷酸合成的 HEX 亚磷酰胺，纯 6 异构体。

HEX（六氯荧光素）是一种荧光素衍生物，在黄色光谱范围内发射（最大吸收在 533 nm，最大发射在 549 nm）。

HEX 亚磷酰胺用于合成荧光标记的引物和杂交探针，例如用于 qPCR 的 TaqMan、Molecular Beacon 和 Scorpion。 HEX 最有效地被非荧光 DusQ1 暗猝灭剂猝灭，因为它们的光谱有显着重叠（便于与孔径为 500 Å 的 DusQ1 CPG 500 固体支持物一起使用）。 许多基于毛细管凝胶电泳的自动测序仪都有一个 HEX 检测通道。 因此，当使用荧光标记的正向引物和未标记的反向引物扩增微卫星基因座时，这种亚磷酰胺通常用于合成 5′ 标记的寡核苷酸以进行片段分析，特别是用于微卫星分析。

Usage

Coupling: 3 min.

Deprotection: standard conditions using 25% ammonium; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °С removes all protecting groups from standard nucleobases). AMA (solution of concentrated aqueous ammonium/40% aqueous methylamine 1:1 v/v) can be used with ~5% of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C).

耦合：3 分钟。

脱保护：使用 25% 铵的标准条件； 脱保护时间取决于寡核苷酸组成和核碱基保护基团（在 55°C 下脱保护 17 小时会去除标准核碱基上的所有保护基团）。 AMA（浓铵水溶液/40% 甲胺水溶液 1:1 v/v）可用于形成约 5% 的非荧光副产物。 为避免形成副产物，用氢氧化铵开始脱保护（室温下 30 分钟），然后加入等体积的 40% 甲胺水溶液，并根据需要使用 AMA 继续脱保护（例如，在 65°C 下 10 分钟）。

HEX phosphoramidite, 6-isomer HEX phosphoramidite, 6-isomer HEX phosphoramidite, 6-isomer HEX phosphoramidite, 6-isomer

Absorption and emission spectra of HEX

HEX phosphoramidite, 6-isomer

HEX phosphoramidite, 6-isomer相关产品

Cyanine5 phosphoramidite

Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.

Cy5 的亚磷酰胺衍生物，用于寡核苷酸合成。

BDP TMR azide

Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.

具有叠氮基的硼二吡咯亚甲基染料。 光谱特性类似于 TAMRA 染料。 由于荧光寿命长，BDP TMR 特别适用于荧光偏振测量。

Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

Catalyst for bioconjugate synthesis by Click Chemistry.

Click Chemistry 用于生物共轭合成的催化剂。

General properties

Appearance: off white solid
Molecular weight: 1050.61
CAS number: 1360547-55-2
Molecular formula: C46H52N3Cl6O10P
Solubility: Good solubility in acetonitrile and DCM.
Quality control: NMR 1H and 31P, HPLC-MS
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 87770
Emission maximum, nm: 549
Fluorescence quantum yield: 0.57
CF260: 0.30
CF280: 0.13

Oligo synthesis details

Diluent: anhydrous acetonitrile (prepare a 0.1 М solution, storage 1 week).

FAM-dT phosphoramidite

FAM-dT phosphoramidite,这种亚磷酰胺允许荧光素 (FAM) 在合成过程中通过亚磷酰胺方法引入寡核苷酸序列的任何位置（在链的中间，在 5′ 和 3′ 末端）。

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FAM-dT phosphoramidite

标签：FAM (Fluorescein), Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
13460 100 mg – in stock
23460 250 mg 390.00$ in stock
43460 1 g 1340.00$ in stock
63460 5 g 5400.00$ in stock
83460 10 g please inquire in stock

This phosphoramidite allows fluorescein (FAM) to be introduced into any position in the oligonucleotide sequence during synthesis by the phosphoramidite method (in the middle of the chain, at the 5’- and 3’-terminus). The reagent is a conjugate of deoxythymidine phosphoramidite and 6-isomer of FAM. Modification is performed during oligonucleotide synthesis by substituting standard dT phosphoramidite with fluorescein-dT phosphoramidite. This modification does not affect exonuclease or polymerase activity.

For modification with fluorescein at the 5’-terminus use FAM phosphoramidite, 6-isomer.

这种亚磷酰胺允许荧光素 (FAM) 在合成过程中通过亚磷酰胺方法引入寡核苷酸序列的任何位置（在链的中间，在 5′ 和 3′ 末端）。 该试剂是脱氧胸苷亚磷酰胺和 FAM 6-异构体的结合物。 在寡核苷酸合成过程中通过用荧光素-dT 亚磷酰胺代替标准 dT 亚磷酰胺进行修饰。 这种修饰不影响核酸外切酶或聚合酶活性。

要在 5′-末端使用荧光素进行修饰，请使用 FAM 亚磷酰胺，6-异构体。

Usage

Coupling: 10 minutes

Deprotection: standard conditions using ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °С removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C).

耦合：10 分钟

脱保护：使用氢氧化铵的标准条件； 脱保护时间取决于寡核苷酸组成和核碱基保护基团（在 55°С 脱保护 17 小时会去除标准核碱基上的所有保护基团）。 AMA（30% 氢氧化铵/40% 甲胺水溶液 1:1 v/v 的溶液）可用于形成约 5% 的非荧光副产物。 为避免形成副产物，用氢氧化铵开始脱保护（室温下 30 分钟），然后加入等体积的 40% 甲胺水溶液，并根据需要使用 AMA 继续脱保护（例如，在 65°C 下 10 分钟）。

FAM-dT phosphoramidite FAM-dT phosphoramidite FAM-dT phosphoramidite FAM-dT phosphoramidite

Absorption and emission spectra of FAM

FAM-dT phosphoramidite

FAM-dT phosphoramidite相关产品

Cyanine5 azide

Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.

Cy5 azide 是一种用 Cyanine5（一种流行的荧光染料）进行 Click Chemistry 标记的试剂。

Alkyne dT phosphoramidite

Phosphoramidite modifier for the synthesis of oligonucleotides containing alkyne modification in the middle of the chain.

亚磷酰胺修饰剂，用于合成在链中间含有炔烃修饰的寡核苷酸。

DusQ1 amidite, 5′-terminal

General properties

Appearance: off white powder
Molecular weight: 1423.54
Molecular formula: C79H87N6O17P
Solubility: good in acetonitrile, DCM
Quality control: NMR 1H, NMR 31P, HPLC-MS
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 513
Fluorescence quantum yield: 0.93
CF260: 0.20
CF280: 0.17

Oligo synthesis details

Diluent: acetonitrile

FAM Phosphoramidite, 6-Isomer

FAM Phosphoramidite, 6-Isomer,Sandard fluorescein (FAM) 亚磷酰胺，用于 5′-末端寡核苷酸标记，高异构纯度单一异构体。 这种高度纯化的亚酰胺试剂可确保与各种合成器获得出色的偶联效果。

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FAM Phosphoramidite, 6-Isomer

标签：FAM (Fluorescein), Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
D5160 250 mg – in stock
F5160 1 g 190.00$ in stock
G5160 5 g 740.00$ in stock
H5160 10 g 1290.00$ in stock
K5160 50 g please inquire 21 days

Sandard fluorescein (FAM) phosphoramidite for 5′-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.

Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.

Sandard fluorescein (FAM) 亚磷酰胺，用于 5′-末端寡核苷酸标记，高异构纯度单一异构体。 这种高度纯化的亚酰胺试剂可确保与各种合成器获得出色的偶联效果。

寡核苷酸制造商可提供样品和特别优惠。 联系我们获取该试剂的批量供应。

FAM Phosphoramidite, 6-Isomer FAM Phosphoramidite, 6-Isomer FAM Phosphoramidite, 6-Isomer FAM Phosphoramidite, 6-Isomer

FAM absorbance and emission spectra

FAM Phosphoramidite, 6-Isomer

FAM Phosphoramidite, 6-Isomer相关产品

Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

Catalyst for bioconjugate synthesis by Click Chemistry.

Click Chemistry 用于生物共轭合成的催化剂。

Phosphate CPG 1000 solid support

Phosphate modifier CPG for the solid phase synthesis of long (up to 120mer) oligonucleotides containing 3 prime phosphate modification.

磷酸盐修饰剂 CPG，用于固相合成含有 3 个磷酸基修饰的长（最多 120 聚体）寡核苷酸。

BDP FL azide

BDP FL is a bright and photostable borondipyrromethene dye for fluorescein channel. This is a dye azide for Click Chemistry.

BDP FL 是一种用于荧光素通道的明亮且光稳定的硼二吡咯亚甲基染料。 这是一种用于 Click Chemistry 的染料叠氮化物。

General properties

Appearance: white foam
Molecular weight: 843.94
CAS number: 204697-37-0
Molecular formula: C46H58N3O10P
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下最多保存 2 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: coupling time 10 min
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine

相关文献：

  1. Zhou, Z.; Liu, S.; Zhang, Y.; Yang, X.; Ma, Y.; Guan, Z.; Wu, Y.; Zhang, L.; Yang, Z. Reductive nanocomplex encapsulation of cRGD-siRNA conjugates for enhanced targeting to cancer cells. International Journal of Nanomedicine, 2017, 12, 7255–7272. doi: 10.2147/ijn.S136726

FAM Phosphoramidite, 5-Isomer

FAM Phosphoramidite, 5-Isomer,用于 5′-末端寡核苷酸标记的标准荧光素 (FAM) 亚磷酰胺，高异构纯度单一异构体。 这种高度纯化的亚酰胺试剂可确保与各种合成器获得出色的偶联效果。

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  • FAM Phosphoramidite, 5-Isomer

FAM Phosphoramidite, 5-Isomer

标签：FAM (Fluorescein),Oligonucleotide synthesis,Phosphoramidite
货号 规格 价格 货期
2639-500mg 500 mg – 10 days
2639-1g 1 g 190.00$ in stock
2639-5g 5 g 740.00$ in stock
2639-10g 10 g 1290.00$ in stock
2639-50g 50 g please inquire 21 days

Standard fluorescein (FAM) phosphoramidite for 5′-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.

Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.

用于 5′-末端寡核苷酸标记的标准荧光素 (FAM) 亚磷酰胺，高异构纯度单一异构体。 这种高度纯化的亚酰胺试剂可确保与各种合成器获得出色的偶联效果。

寡核苷酸制造商可提供样品和特别优惠。 联系我们获取该试剂的批量供应。

FAM Phosphoramidite, 5-Isomer FAM Phosphoramidite, 5-Isomer FAM Phosphoramidite, 5-Isomer

FAM Phosphoramidite, 5-Isomer相关产品

Phosphate CPG 500 solid support

CPG solid support for the synthesis of 3′-phosphorylated oligonucleotides.

用于合成 3′-磷酸化寡核苷酸的 CPG 固体支持物。

HEX phosphoramidite, 6-isomer

HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.

用于合成 5′ 标记寡核苷酸的 HEX 亚磷酰胺。

FAM Phosphoramidite, 5-Isomer Add this product to your cart and
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General properties

Appearance: off white solid
Molecular weight: 843.94
CAS number: 147566-42-5
Molecular formula: C46H58N3O10P
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: coupling time 10 min
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine

相关文献：

  1. Petrunina, N.A.; Lebedev, V.V.; Kirillova, Y.G.; Aralov, A..V.; Varizhuk, A.M.; Sardushkin, M.V. DNA Intercalated Motifs with Non-Nucleoside Inserts. Russian Journal of Bioorganic Chemistry, 2021, 47(6), 1341–1344. doi: 10.1134/S1068162021060212

DusQ2 phosphoramidite

DusQ2 phosphoramidite,DusQ2 是一种黑色猝灭剂染料，用于合成带有 5′-猝灭剂的 qPCR 的双标记寡核苷酸探针。 这种猝灭剂非常适合 HEX、JOE、ROX、Cyanine5 和其他在光谱的橙色和红色部分发射的染料。

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  • DusQ2 phosphoramidite

DusQ2 phosphoramidite

标签：Oligonucleotide synthesis,Phosphoramidite
货号 规格 价格 货期
1473-100mg 100 mg 290.00$ in stock
1473-500mg 500 mg 990.00$ in stock
1473-1g 1 g 1690.00$ in stock
1473-10g 10 g please inquire 21 days

DusQ2 is a black quencher dye for the synthesis of dual labeled oligonucleotide probes for qPCR bearing 5′-quencher. This quencher is ideal for HEX, JOE, ROX, Cyanine5 and other dyes with the emission in orange and red part of the spectrum.

The primary phosphoramidite group couples with hydroxyls efficiently in oligonucleotide synthesis.

DusQ2 是一种黑色猝灭剂染料，用于合成带有 5′-猝灭剂的 qPCR 的双标记寡核苷酸探针。 这种猝灭剂非常适合 HEX、JOE、ROX、Cyanine5 和其他在光谱的橙色和红色部分发射的染料。

初级亚磷酰胺基团在寡核苷酸合成中有效地与羟基偶联。

DusQ2 phosphoramidite DusQ2 phosphoramidite DusQ2 phosphoramidite DusQ2 phosphoramidite

Absorption spectrum of DusQ2

DusQ2 phosphoramidite

DusQ2 phosphoramidite相关产品

R6G azide, 5-isomer

Rhodamine 6G is a xanthene dye that has been used for DNA sequencing and qPCR. This azide derivative is used for the attachment of the dye to DNA and other molecules by means of Click chemistry.

罗丹明 6G 是一种已用于 DNA 测序和 qPCR 的呫吨染料。 这种叠氮化物衍生物用于通过点击化学将染料连接到 DNA 和其他分子上。

DusQ1 phosphoramidite

Cyanine5 phosphoramidite

Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.

Cy5 的亚磷酰胺衍生物，用于寡核苷酸合成。

General properties

Appearance: dark colored solid
Molecular weight: 997.08
CAS number: 374591-98-7
Molecular formula: C54H61N8O9P
Quality control: NMR 1H, 31P, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 552
ε, L⋅mol−1⋅cm−1: 34000
CF260: 0.31
CF280: 0.26

Oligo synthesis details

Diluent: 20% THF / acetonitrile
Coupling conditions: 6 min coupling time; 3 min oxidation time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases

DusQ2 dT phosphoramidite

DusQ2 dT phosphoramidite,

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DusQ2 dT phosphoramidite

标签：Oligonucleotide synthesis,Phosphoramidite
货号 规格 价格 货号
1451-100mg 100 mg 340.00$ in stock
1451-500mg 500 mg 1390.00$ in stock
1451-1g 1 g 2290.00$ in stock

DusQ2 dT phosphoramidite相关产品

Cyanine5 azide

Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.

Cy5 azide 是一种用 Cy5（一种流行的荧光染料）进行 Click Chemistry 标记的试剂。

FAM Phosphoramidite, 5-Isomer

Single isomer 5-FAM phosphoramidite for the synthesis of modified oligonucleotides.

用于合成修饰寡核苷酸的单一异构体 5-FAM 亚磷酰胺。

FAM-dT phosphoramidite

Phosphoramidite for synthesis of oligonucleotides labeled with fluorescein (FAM) in the middle of sequence.

亚磷酰胺用于合成在序列中间用荧光素 (FAM) 标记的寡核苷酸。

General properties

Appearance: черный порошок
Molecular weight: 1,413.55
Quality control: NMR 1H, 31P,HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Coupling conditions: 6 min coupling time; 3 min oxidation time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases

DusQ1 phosphoramidite

DusQ1 phosphoramidite,Oligonucleotide synthesis, Phosphoramidite

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DusQ1 phosphoramidite

标签：Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
1470-100mg 100 mg 290.00$ in stock
1470-500mg 500 mg 990.00$ in stock
1470-1g 1 g 1690.00$ in stock

DusQ1 phosphoramidite相关产品

Alkyne dT phosphoramidite

Phosphoramidite modifier for the synthesis of oligonucleotides containing alkyne modification in the middle of the chain.

亚磷酰胺修饰剂，用于合成在链中间含有炔烃修饰的寡核苷酸。

Cyanine5.5 azide

Far red / near infrared fluorescent Cyanine5.5 dye derivative for Click Chemistry.

用于 Click Chemistry 的远红/近红外荧光 Cy5.5 染料衍生物。

DusQ1 phosphoramidite Add this product to your cart and
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DusQ2 dT phosphoramidite

General properties

Appearance: black foam
Molecular weight: 995.11
CAS number: 1430816-77-5
Quality control: NMR 1H, 31P,HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Oligo synthesis details

Diluent: 20% THF / acetonitrile
Coupling conditions: 6 min coupling time; 3 min oxidation time
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases

DusQ1 phosphoramidite

DusQ1 dT phosphoramidite

DusQ1 dT phosphoramidite,耦合条件,6分钟耦合时间； 3分钟氧化时间,裂解条件,氨水，室温下 2 小时.

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  • DusQ1 dT phosphoramidite

DusQ1 dT phosphoramidite

标签：Oligonucleotide synthesis,Phosphoramidite
货号 规格 价格 货期
1452-100mg 100 mg 340.00$ in stock
1452-500mg 500 mg 1390.00$ 10 days
1452-1g 1 g 2290.00$ in stock

DusQ1 dT phosphoramidite相关产品

Alkyne CPG modifier 500

Solid support for the synthesis of 3′-modified oligonucleotides with alkyne group for Click Chemistry.

为 Click Chemistry 合成具有炔基的 3′-修饰寡核苷酸提供坚实支持。

Alkyne Phosphoramidite, 5′-terminal

Alkyne phosphoramidite for the synthesis of oligonucleotides ready for Click chemistry.

用于合成寡核苷酸的炔亚磷酰胺，可用于点击化学。

DusQ1 dT phosphoramidite Add this product to your cart and
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DusQ1 CPG 500

Controlled pore glass (CPG) 500 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.

可控孔玻璃 (CPG) 500 固体支持物，用于合成具有 3′-末端 DusQ1 淬灭剂的寡核苷酸。 与标准解块条件兼容。

General properties

Appearance: черный порошок 黑火药
Molecular weight: 1,411.58
Quality control: NMR 1H, 31P,HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Oligo synthesis details

Coupling conditions:

耦合条件

 6 min coupling time; 3 min oxidation time

6分钟耦合时间； 3分钟氧化时间
Cleavage conditions:

裂解条件

 ammonia, 2 h at room temperature

氨水，室温下 2 小时
Deprotection conditions: identical to protected nucleobases

与受保护的核碱基相同

DusQ1 amidite, 5′-terminal

DusQ1 amidite, 5′-terminal,储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

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  • DusQ1 amidite, 5′-terminal

DusQ1 amidite, 5′-terminal

标签：Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
1D260 100 mg 290.00$ in stock
2D260 250 mg 590.00$ in stock
3D260 500 mg 990.00$ in stock
4D260 1 g 1690.00$ in stock
6D260 5 g 6790.00$ in stock
7D260 10 g please inquire 10 days

Absorption spectrum of DusQ1

DusQ1 amidite, 5'-terminal

DusQ1 amidite, 5′-terminal相关产品

DusQ1 phosphoramidite

FAM-dT phosphoramidite

Phosphoramidite for synthesis of oligonucleotides labeled with fluorescein (FAM) in the middle of sequence.

DusQ1 amidite, 5'-terminal Add this product to your cart and
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dsGreen Gel Staining Solution, 10000×

dsGreen for staining dsDNA in gels.

General properties

Appearance: dark colored solid
Molecular weight: 676.75
Molecular formula: C34H45N8O5P
Solubility: good in acetonitrile
Quality control: NMR 1H, 31P, HPLC-MS (95%), coupling test
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 522
ε, L⋅mol−1⋅cm−1: 35935
CF260: 0.17
CF280: 0.10

Oligo synthesis details

Diluent: acetonitrile

Cyanine5.5 phosphoramidite, 5′-terminal

Cyanine5.5 phosphoramidite, 5′-terminal,Cy5.5 是一种荧光团，在光谱的远红色范围内发射。 这种染料可用于多重 qPCR。 商用六通道 qPCR 仪器通常有一个用于 Cy5.5 的通道。

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  • Cyanine5.5 phosphoramidite, 5′-terminal

Cyanine5.5 phosphoramidite, 5′-terminal

标签：Cyanine5.5, Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
2680-100mg 100 mg – 7 days
2680-250mg 250 mg 290.00$ 10 days
2680-1g 1 g 950.00$ in stock
2680-10g 10 g 6750.00$ 21 days
Cyanine5.5 is a fluorophore with the emission in far red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine5.5.

This phosphoramidite can be used for the synthesis of 5′-labeled oligonucleotides by direct labeling in oligonucleotide synthesizer.

The structure of phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintaining coupling performance over a longer storage time in oligonucleotide synthesizer, compared to phosphoramidites derived of primary alcohols.

Cy5.5 是一种荧光团，在光谱的远红色范围内发射。 这种染料可用于多重 qPCR。 商用六通道 qPCR 仪器通常有一个用于 Cy5.5 的通道。
该亚磷酰胺可用于通过在寡核苷酸合成仪中直接标记来合成5′-标记的寡核苷酸。

与仲碳原子相连的亚磷酰胺官能团结构提供了额外的稳定性，可防止 Arbuzov 重排。 与衍生自伯醇的亚磷酰胺相比，这有助于在寡核苷酸合成仪中在较长的存储时间内保持偶联性能。

Absorption and emission spectra of Cyanine5.5

Cyanine5.5 phosphoramidite, 5'-terminal

Cyanine5.5 phosphoramidite, 5′-terminal相关产品

Phosphate CPG 1000 solid support

Phosphate modifier CPG for the solid phase synthesis of long (up to 120mer) oligonucleotides containing 3 prime phosphate modification.

磷酸盐修饰剂 CPG，用于固相合成含有 3 个磷酸基修饰的长（最多 120 聚体）寡核苷酸。

Cyanine5 phosphoramidite

Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.

Cy5 的亚磷酰胺衍生物，用于寡核苷酸合成。

FAM CPG 500, 6-isomer

Controlled pore glass for the synthesis of modified oligonucleotides containing 3′-fluorescein (FAM) modification.

用于合成含有 3′-荧光素 (FAM) 修饰的修饰寡核苷酸的可控孔玻璃。

General properties

Appearance: dark colored solid
Molecular weight: 916.61
Molecular formula: C55H71N5ClO3P
Quality control: NMR 1H, 31P, HPLC-MS (85%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 694
ε, L⋅mol−1⋅cm−1: 198000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible

Cyanine5.5 phosphoramidite, 5'-terminal

Cyanine3 phosphoramidite 5′

Cyanine3 phosphoramidite 5′,Cy3 是一种荧光团，广泛用于分子生物学实验，例如寡核苷酸标记和寡核苷酸检测。根据其光谱特性，Cyanine3 是 Cy™3 的类似物，在黄色光谱范围内的荧光最大值为 570 nm。

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Cyanine3 phosphoramidite 5′

标签：Cy3, Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
2771-100mg 100 mg – in stock
2771-250mg 250 mg 290.00$ 10 days
2771-1g 1 g 950.00$ in stock
2771-10g 10 g 6750.00$ 21 days

Cyanine3 is a fluorophore that is widely used in molecular biology experiments such as oligonucleotide labeling followed by oligonucleotide detection. By its spectral characteristics, Cyanine3 is an analog of Cy™3 with a fluorescence maximum at 570 nm in the yellow spectrum range.

Cyanine3 phosphoramidite 5’ is used in oligonucleotide synthesis for the production of 5’-cyanine3-labeled oligonucleotides. The reagent is compatible with various oligonucleotide synthesizers.

This phosphoramidite can be used for the synthesis of fluorescence-labeled primers and hybridization probes such as TaqMan and Molecular Beacon. Such labeled probes can be detected in multiplex real-time PCR in the TAMRA channel.

Condensation: 3 min. Use 0.02 M iodine solution at the oxidation step to avoid degradation of the cyanine dye.

Deprotection: At room temperature with 30% aqueous ammonium solution. It is recommended to use nucleic bases with labile protective groups for deprotection for not more than 2 h at less than 55°C. AMA (30% aqueous ammonium solution/40% aqueous methylamine 1:1 (v/v)) can be used for 10 min at 65°C in the presence of acetyldeoxycytidine. If deoxyguanidine with a dimethylformamidine protective group is used during synthesis, deprotect with 30% aqueous ammonium solution for 2 h at 65°C. If deoxyguanidine with an isobutyryl protective group is used during synthesis, deprotect for 24-36 h at room temperature.

Cy3 是一种荧光团，广泛用于分子生物学实验，例如寡核苷酸标记和寡核苷酸检测。根据其光谱特性，Cyanine3 是 Cy™3 的类似物，在黄色光谱范围内的荧光最大值为 570 nm。

花青 3 亚磷酰胺 5′ 用于寡核苷酸合成，以生产 5′-花青 3 标记的寡核苷酸。该试剂与各种寡核苷酸合成仪兼容。

这种亚磷酰胺可用于合成荧光标记的引物和杂交探针，例如 TaqMan 和 Molecular Beacon。可以在 TAMRA 通道中的多重实时 PCR 中检测到此类标记的探针。

冷凝：3 分钟。在氧化步骤中使用 0.02 碘溶液以避免花青染料降解。

脱保护：在室温下用 30% 的铵水溶液。建议使用带有不稳定保护基团的核酸碱基在低于 55°C 的条件下脱保护不超过 2 小时。 AMA（30% 铵水溶液/40% 甲胺水溶液 1:1 (v/v)）可在乙酰脱氧胞苷存在下在 65°C 下使用 10 分钟。如果在合成过程中使用带有二甲基甲脒保护基团的脱氧胍，则在 65°C 下用 30% 铵水溶液脱保护 2 小时。如果在合成过程中使用带有异丁酰基保护基团的脱氧胍，则在室温下脱保护 24-36 小时。

Cyanine3 phosphoramidite 5’相关产品

Cyanine5.5 phosphoramidite, 5′-terminal

Amidite for the synthesis of oligonucleotides and qPCR probes bearing 5′-terminal dye Cyanine5.5. Useful dye for multiplex qPCR.

Amidite 用于合成带有 5′-末端染料 Cyanine5.5 的寡核苷酸和 qPCR 探针。 用于多重 qPCR 的有用染料。

FAM Phosphoramidite, 6-Isomer

Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.

用于合成修饰寡核苷酸的单一异构体 6-FAM 亚磷酰胺。

Cyanine5 phosphoramidite

Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.

Cyanine5 的亚磷酰胺衍生物，用于寡核苷酸合成。

General properties

Appearance: red powder
Molecular weight: 841.81
Molecular formula: C45H65N5BF4O3P
Solubility: good in acetonitrile, dichloromethane
Quality control: NMR 1H, 31P, HPLC-MS (80%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible

建议在 +4°C 或超温和保护基团下放置 48 小时； 24 小时在 rt 可能
Cy™ is a registered trademark of Cytiva in some countries.

Chemical phosphorylation reagent for oligonucleotide synthesis

Chemical phosphorylation reagent for oligonucleotide synthesis,用于合成 5′-磷酸化寡核苷酸的化学磷酸化试剂。 该试剂的结构中含有一个 DMT 基团，可以在 C18 小柱上或通过反相色谱法纯化寡核苷酸。 要产生 5′-磷酸化寡核苷酸，请去除 DMT 基团，然后用稀释的铵溶液 (0.1 M) 去保护磷酸基团。 即使在温和的碱性条件下，磷酸基团的脱保护也能快速有效地进行，因此该试剂可用于 DMT-Off 合成，例如 RNA 合成，或用于需要温和脱保护条件的染料亚磷酰胺。

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Chemical phosphorylation reagent for oligonucleotide synthesis

标签：Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
21560 250 mg – in stock
41560 1 g 450.00$ in stock
61560 5 g 1990.00$ in stock

Chemical phosphorylation reagent for the synthesis of 5’-phosphorylated oligonucleotides. The reagent contains a DMT group in its structure, which allows oligonucleotide purification on C18-cartridges or by reversed-phase chromatography. To yield 5’-phosphorylated oligonucleotide, remove DMT group and then deprotect the phosphate group with diluted ammonium solution (0.1 M). Deprotection of the phosphate group is rapid and efficient even under mild basic conditions, so this reagent can be used for DMT-Off synthesis, for example for RNA synthesis, or with dye phosphoramidites that require mild deprotection conditions.

用于合成 5′-磷酸化寡核苷酸的化学磷酸化试剂。 该试剂的结构中含有一个 DMT 基团，可以在 C18 小柱上或通过反相色谱法纯化寡核苷酸。 要产生 5′-磷酸化寡核苷酸，请去除 DMT 基团，然后用稀释的铵溶液 (0.1 M) 去保护磷酸基团。 即使在温和的碱性条件下，磷酸基团的脱保护也能快速有效地进行，因此该试剂可用于 DMT-Off 合成，例如 RNA 合成，或用于需要温和脱保护条件的染料亚磷酰胺。

Usage

Diluent: anhydrous acetonitrile. Add the diluent to the recommended concentration (0.1 M) and wait until reagent dissolution is complete while mixing periodically. This reagent is viscous amorphous, and it may take up to 10 minutes to dissolve it. Store the diluted reagent in anhydrous conditions for not more than 24 hours.

稀释剂：无水乙腈。 将稀释剂添加至推荐浓度 (0.1 M) 并等待直到试剂溶解完成，同时定期混合。 该试剂是粘性无定形的，可能需要 10 分钟才能溶解。 将稀释后的试剂在无水条件下存放不超过 24 小时。

Coupling: 6 minutes

耦合：6 分钟

Deprotection脱保护:

  1. DMT group is removed during synthesis: to yield the 5’-terminal phosphate, deprotect under standard conditions using ammonium hydroxide.DMT 基团在合成过程中被去除：为了产生 5′-末端磷酸盐，在标准条件下使用氢氧化铵去保护。
  2. DMT-ON, cartridge purification: standard cartridge purification conditions. After elution from the cartridge, deprotect the phosphate group by adding an equivalent volume of 25% aqueous ammonium solution to oligonucleotide solution, incubate for 15 minutes at room temperature, and dry the oligonucleotide down.DMT-ON，柱净化：标准柱净化条件。 从小柱中洗脱后，通过向寡核苷酸溶液中加入等体积的 25% 铵水溶液来脱去磷酸基团的保护，在室温下孵育 15 分钟，然后将寡核苷酸干燥。
  3. DMT-ON, purification by HPLC: purify 5’-DMT-oligonucleotide by reversed-phase HPLC. To remove DMT group, redissolve oligonucleotide in 80% acetic acid and incubate for 30 minutes at room temperature. Dry the oligonucleotide down, add 10% aqueous ammonium and incubate for 15 minutes to cleave the remaining fragment of the protecting group.DMT-ON，HPLC 纯化：通过反相 HPLC 纯化 5′-DMT-寡核苷酸。 要去除 DMT 基团，将寡核苷酸重新溶解在 80% 乙酸中并在室温下孵育 30 分钟。 将寡核苷酸干燥，加入 10% 铵水溶液并孵育 15 分钟以切割剩余的保护基片段。
  4. DMT-OFF: to yield the 5’-phosphate, use standard deprotection conditions after synthesis.DMT-OFF：要产生 5′-磷酸，在合成后使用标准脱保护条件。

Chemical phosphorylation reagent for oligonucleotide synthesis相关产品

TAMRA phosphoramidite, 5-isomer

TAMRA phosphoramidite for synthesis of 5’-labeled oligonucleotides. Pure 5-isomer.

TAMRA 亚磷酰胺，用于合成 5′ 标记的寡核苷酸。 纯5-异构体。

dsGreen for Real-Time PCR, 100×

Stock solution of dsGreen for Real-Time PCR, 100×

用于实时 PCR 的 dsGreen 储备溶液，100×

Alkyne Amidite, hydroxyprolinol

Acetylenic amidite for the incorporation of acetylene groups into oligos.

用于将乙炔基团结合到寡核苷酸中的乙炔酰胺。

General properties

Appearance: colorless semisolid
Molecular weight: 722.8
CAS number: 171285-25-9
Molecular formula: C39H51N2O9P
IUPAC name: Propanedioic acid, 2-​[[bis(4-​methoxyphenyl)​phenylmethoxy]​methyl]​-​2-​[[[[bis(1-​methylethyl)​amino]​(2-​cyanoethoxy)​phosphino]​oxy]​methyl]​-​, 1,​3-​diethyl ester
Quality control: NMR 1H,31P, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: recommended coupling time 6 min
Deprotection conditions: identical to protected nucleobases

Chemical phosphorylation reagent for oligonucleotide synthesis

Alkyne Amidite, hydroxyprolinol

Alkyne Amidite, hydroxyprolinol,Phosphoramidite for the synthesis of alkyne-modified oligonucleotides. Oligonucleotides can be used for Click Chemistry modification (see the protocol).

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  • Alkyne Amidite, hydroxyprolinol

Alkyne Amidite, hydroxyprolinol

标签：Alkynes, Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
21360 250 mg 110.00$ in stock
41360 1 g 390.00$ in stock

Phosphoramidite for the synthesis of alkyne-modified oligonucleotides. Oligonucleotides can be used for Click Chemistry modification (see the protocol).

Diluent for this amidite is acetonitrile, 5 min coupling time is recommended. Standard deprotection conditions can be used for oligonucleotides.

Oligonucleotides can be purified by HPLC or cartridges due to the presence of dimethoxytrityl group, as well as by PAGE.

用于合成炔烃修饰的寡核苷酸的亚磷酰胺。 寡核苷酸可用于点击化学修饰（参见协议）。

该亚酰胺的稀释剂是乙腈，建议偶联时间为 5 分钟。 标准脱保护条件可用于寡核苷酸。

由于存在二甲氧基三苯甲基，寡核苷酸可以通过 HPLC 或小柱以及 PAGE 进行纯化。

Alkyne Amidite, hydroxyprolinol相关产品

Cyanine5.5 NHS ester

Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.

Cy5.5 NHS 是远红/近红外胺活性染料。

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3-Ethynyl perylene

Alkyne derivative of ultra-bright and photostable perylene fluorophore.

超亮和光稳定苝荧光团的炔烃衍生物。

Cyanine3 NHS ester

Amine-reactive Cyanine3 dye NHS ester.

胺反应性 Cy3 染料 NHS 酯。

General properties

Appearance: colorless semisolid 无色半固体
Molecular weight: 713.84
CAS number: 1357289-02-1
Molecular formula: C41H52N3O6P
IUPAC name: Phosphoramidous acid, N,​N-​bis(1-​methylethyl)​-​, (3R,​5S)​-​5-​[[bis(4-​methoxyphenyl)​phenylmethoxy]​methyl]​-​1-​(1-​oxo-​5-​hexyn-​1-​yl)​-​3-​pyrrolidinyl 2-​cyanoethyl ester
Solubility: good in acetonitrile and dichloromethane
Quality control: NMR 1H and 31P, HPLC-MS (95 %)
Storage conditions: Storage: 12 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

储存：-20°C 收货后 12 个月。 运输：在室温下长达 3 周。 干燥。
MSDS: Download
Product specifications

Oligo synthesis details

Diluent: acetonitrile