ProSpec,NT5E (CD73) Mouse,5′-核苷酸酶体外小鼠重组PRO-2386

ProSpec,NT5E (CD73) Mouse,5′-核苷酸酶体外小鼠重组PRO-2386,在 Sf9 杆状病毒细胞中产生的 NT5E 小鼠重组体是单条糖基化多肽链,含有 531 个氨基酸,分子量为 59.1kDa。

  • NT5E Mouse

  • 5′-Nucleotidase Ecto Mouse Recombinant
  • PRO-2386

Catalogue number

PRO-2386

Synonyms

5′-nucleotidase , 5′-NT, Ecto-5′-nucleotidase, CD73, Nt5e, Nt5, Nte.

Introduction

NT5E, also known as CD73, is a plasma membrane protein which catalyzes the conversion of extracellular nucleotides into membrane-permeable nucleosides. NT5E is functions as a determinant of lymphocyte differentiation. Defects in NT5E can cause the calcification of joints and arteries.
NT5E,也称为 CD73,是一种质膜蛋白,可催化细胞外核苷酸转化为可透过膜的核苷。 NT5E 是淋巴细胞分化的决定因素。 NT5E 的缺陷会导致关节和动脉的钙化。

Description

NT5E Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 531 amino acids and having a molecular mass of 59.1kDa .
NT5E is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 NT5E 小鼠重组体是单条糖基化多肽链,含有 531 个氨基酸,分子量为 59.1kDa。
NT5E 在 C 末端用 6 个氨基酸 His 标签表达,并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

NT5E protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

Purity

Greater than 90% as determined by -PAGE.

Biological Activity

Specific activity is > 8,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25°C.

Amino acid sequence

WELTILHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLFLDAGDQ YQGTIWFTVY KGLEVAHFMN ILGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAHQISG LFLPSKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE ISALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DIVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTADDGRQVP VVQAYAFGKY LGYLKVEFDD KGNVITSYGN PILLNSSIPE DATIKADINQ WRIKLDNYST QELGRTIVYL DGSTQTCRFR ECNMGNLICD AMINNNLRHP DEMFWNHVSM CIVNGGGIRS PIDEKNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDINRKPW NRVVQLEVLC TKCRVPIYEP LEMDKVYKVT LPSYLANGGD GFQMIKDELL KHDSGDQDIS VVSEYISKMK VVYPAVEGRI KFSLEHHHHH H.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • CD73 Antibody
  • NT5E Human