标签： MIP 3a Mouse

Small inducible cytokine A20 precursor, CCL20, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Liver and activation- regulated chemokine, CC chemokine LARC, Beta chemokine exodus-1, CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20, C-C motif chemokine 20, CC chemokine ST38, exodus-1.

CCL20 is a chemotactic factor that draws lymphocytes & neutrophils, rather than monocytes. MIP-3 alpha inhibits proliferation of myeloid progenitors in colony formation assays. MIP3A plays a role in the formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. CCL20 holdes antibacterial activity e.coli atcc 25922 and s.aureus atcc 29213. CCL20 gene transcription is activated by H. pylori, which activates NF-kappaB through intracellular signal pathway which involves IkappaB kinase and NF-kappaB-inducing kinase. MIP-3 alpha is invloved in chemokine-mediated lymphocyte trafficking during gastric inflammation in Helicobacter infection. CCL20 expression is involved in the recruitment of CD45R0-positive T cell subsets into the intestinal lamina propria.
MIP-3A is in charge of the advancement of pulpal inflammation through the recruitment of C-C motif Receptor 6-expressing lymphocytes Vaginal epithelial cells respond to factors present in semen by secreting MIP-3 alpha, which increases langerhans cells recruitment during HIV transmission.

CCL20 是一种趋化因子，可吸引淋巴细胞和中性粒细胞，而不是单核细胞。 MIP-3 α 在集落形成试验中抑制骨髓祖细胞的增殖。 MIP3A 通过将淋巴细胞和树突状细胞吸引到上皮细胞，在黏膜淋巴组织的形成和功能中发挥作用。 C-末端加工形式已被证明对白细胞具有同样的趋化活性。 CCL20 具有抗菌活性 e.coli atcc 25922 和 s.aureus atcc 29213。CCL20 基因转录由幽门螺杆菌激活，幽门螺杆菌通过涉及 IkappaB 激酶和 NF-kappaB 诱导激酶的细胞内信号通路激活 NF-kappaB。 MIP-3 α 在幽门螺杆菌感染的胃炎症期间参与趋化因子介导的淋巴细胞运输。 CCL20 表达参与将 CD45R0 阳性 T 细胞亚群募集到肠固有层中。
MIP-3A 负责通过募集 C-C 基序受体 6 表达淋巴细胞来促进牙髓炎症阴道上皮细胞通过分泌 MIP-3 α 对精液中存在的因子作出反应，这增加了 HIV 传播过程中朗格汉斯细胞的募集。

Macrophage Inflammatory Protein-3 alpha Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.9 kDa.
The MIP-3a is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a concentrated solution in water containing no additives.

It is recommended to reconstitute the lyophilized MIP-3a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized MIP-3a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL-20 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 95.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

ASNYDCCLSY IQTPLPSRAI VGFTRQMADE ACDINAIIFH TKKRKSVCAD PKQNWVKRAV NLLSLRVKKM.

The Activity is calculated by its ability to chemoattract primary human T cells at 2-20ng/ml, corresponding to a specific activity of 50,000-500,000 units/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.