标签： MCP 5 Mouse

C-C motif chemokine 12, MCP-1-related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, MCP-5, Small-inducible cytokine A12, Ccl12, Mcp5, Scya12.

MCP-5 is a cloned mouse CC chemokine most closely related to human MCP1 . MCP5 is expressed constitutively in the thymus and the lymph nodes. Under inflammatory conditions, moreover CCL12 expression is induced in activated macrophages and mast cells. The mouse MCP1 gene is mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 is a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, MCP-5 also chemoattracts eosinophils. CCL12 is a functional ligand for CCR2.

MCP-5 是与人类 MCP1 最密切相关的克隆小鼠 CC 趋化因子。 MCP5 在胸腺和淋巴结中组成型表达。 此外，在炎症条件下，CCL12 表达在活化的巨噬细胞和肥大细胞中被诱导。 小鼠 MCP1 基因映射到 11 号染色体上的 CC 趋化因子簇。重组 CCL12 是单核细胞和淋巴细胞的有效趋化因子，但对中性粒细胞无效。 在高浓度时，MCP-5 也会化学吸引嗜酸性粒细胞。 CCL12 是 CCR2 的功能性配体。

MCP-5 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 82 amino acids and having a molecular mass of 9.3kDa.
The MCP5 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 MCP-5 Mouse Recombinant 是一种单一的、非糖基化的多肽链，含有 82 个氨基酸，分子量为 9.3kDa。
MCP5 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.

It is recommended to reconstitute the lyophilized MCP5 in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized CCL12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCP5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 CCL12 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，MCP5 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG.

Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0-50.0 ng/ml.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.