标签： MCP 2 Human

Small inducible cytokine A8, CCL8, Monocyte chemotactic protein 2, MCP-2, Monocyte chemoattractant protein 2, HC14, chemokine ligand 8, MCP2, SCYA8, SCYA10.

Chemokine ligand 8 is a small cytokine belonging to the CC chemokine family that was once called monocyte chemotactic protein-2 . The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. MCP-2 is chemotactic for and activates a many different immune cells, including mast cells, eosinophils and basophils, , and monocytes, T cells, and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different cell surface receptors called chemokine receptors. These receptors include CCR1, CCR2B and CCR5.

趋化因子配体 8 是一种小细胞因子，属于 CC 趋化因子家族，曾被称为单核细胞趋化蛋白 2。 CCL8 蛋白作为含有 109 个氨基酸的前体产生，其被切割产生含有 75 个氨基酸的成熟 CCL8。 CCL8 基因由 3 个外显子编码，位于人类染色体 17q11.2 上的一大簇 CC 趋化因子中。 MCP-2 趋化并激活许多不同的免疫细胞，包括肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞，以及参与炎症反应的单核细胞、T 细胞和 NK 细胞。 CCL8 通过与几种不同的细胞表面受体（称为趋化因子受体）结合来引发其作用。 这些受体包括 CCR1、CCR2B 和 CCR5。

Monocyte Chemotactic Protein-2 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8904 Dalton.
The MCP2 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的单核细胞趋化蛋白 2 人重组体是一种非糖基化的多肽链，含有 76 个氨基酸，分子量为 8904 道尔顿。
MCP2 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The protein was lyophilized from a concentrated sterile solution containing no additives.

It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-2in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized MCP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL8should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ser-Val.

The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium with human THP-1 cells. Significant calcium mobilization is observed with 500ng/mL of recombinant human MCP-2. Human MCP-2 also induces dose dependent chemotaxis of human THP-1 cells with an ED50=30-100 ng/mL  corresponding to a Specific Activity of 10,000-33,334IU/mg.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.