标签： Lungkine 小鼠重组体CHM-286

C-X-C motif chemokine 15, Lungkine, Small-inducible cytokine B15, Cxcl15, Scyb15.

Mouse Lungkine/CXCL15 belongs to the ELR motif-containing CXC chemokines. The mouse Lungkine gene has been mapped to chromosome 5. The cDNA of mouse Lungkine encodes a 166 amino acids protein with a 25 aa predicted signal peptide and a 141 aa mature protein with an exceptionally long C-terminal tail which extends beyond beyond the chemokine fold. Lungkine protein is secreted into bronchoalveolar space and is involved in lung-specific neutrophils trafficking. Furthermore, studies in Lungkine knockout mice propose that Lungkine is an imperative mediator of neutrophil migration from the lung parenchyma into the airspace. In addition, Lungkine is chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation. By Northern blot analysis and in-situ hybridization, Lungkine transcripts have only been specifically detected in the adult and fetal lung, and its expression is up-regulated under inflammatory conditions. There is a 35% aa sequence similarity between the mouse Lungkine and the human ENA-78 and a 31% similarity with the human IL-8.

小鼠 Lungkine/CXCL15 属于含有 ELR 基序的 CXC 趋化因子。小鼠 Lungkine 基因已被定位到 5 号染色体。小鼠 Lungkine 的 cDNA 编码一个 166 个氨基酸的蛋白质，具有 25 个氨基酸的预测信号肽和一个 141 个氨基酸的成熟蛋白质，其 C 末端尾部非常长，延伸到趋化因子折叠之外. Lungkine 蛋白分泌到支气管肺泡空间并参与肺特异性嗜中性粒细胞运输。此外，对 Lungkine 基因敲除小鼠的研究表明，Lungkine 是中性粒细胞从肺实质迁移到空气空间的必要介质。此外，Lungkine 对骨髓祖细胞具有趋化作用并调节造血细胞分化。通过 Northern 印迹分析和原位杂交，仅在成人和胎儿肺中特异性检测到 Lungkine 转录物，并且其表达在炎症条件下上调。小鼠 Lungkine 与人类 ENA-78 之间的 aa 序列相似性为 35%，与人类 IL-8 的相似性为 31%。

Recombinant Mouse Lungkine produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 142 amino acids and having a molecular mass of 16.4kDa.
The CXCL15 is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The Lungkine protein was lyophilized from a 0.2µm filtered concentrated solution in PBS pH 7.4 and 0.02% Tween-20.

It is recommended to reconstitute the lyophilized Lungkine in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18M-cm H2O 中重新配制冻干的 Lungkine，然后可以将其进一步稀释成其他水溶液。

Lyophilized Lungkine although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL15 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 Lungkine 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CXCL15 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

QELRCLCIQE HSEFIPLKLI KNIMVIFETI YCNRKEVIAV PKNGSMICLD PDAPWVKATV GPITNRFLPE DLKQKEFPPA MKLLYSVEHE KPLYLSFGRP ENKRIFPFPI RETSRHFADL AHNSDRNFLR DSSEVSLTGS DA.

The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 20-100 ng/ml.

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