DiO, also called DiOC18(3), is a green fluorescent, lipophilic carbocyanine dye. DiO’s excitation max is 484 nm, and emission max is 501 nm.
DiO is widely used as an anterograde and retrograde neuronal tracer in living and fixed tissues and cells. DiO uniformly labels neurons via diffusion in the plasma membrane. In intact tissue, the dye does not transfer from labeled to unlabeled cells but some transfer may occur when the membrane is disrupted, for example after sectioning.
DiO and DiI are often used together in dual-color studies. But DiO has a slower lateral diffusion rate on the membranes than that of DiI.
DusQ3 is a dark quencher for far red and near infrared fluorophores, especially suitable for Cyanine5.5.
The dye can be attached to 3′-terminus of oligonucleotides by means of automated solid phase synthesis using this support. The dye is sensitive to nucleophilic reagents, and therefore must be carefully deprotected. We recommend the use of ultramild, phenoxyacetyl protected monomers. As an alternative, post-synthetic labeling using NHS ester of the quencher can be used.
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DusQ1 CPG 500
Controlled pore glass (CPG) 500 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.
DusQ2 CPG 500
Controlled pore glass (CPG) 500 modified support for the synthesis of oligonucleotides with DusQ2 quencher at the 3’ end. Compatible with standard deblocking conditions.
Appearance:
dark blue beads
Quality control:
1H NMR of bound reagent, coupling testing with HPLC-MS
Storage conditions:
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS:
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Product specifications
Oligo synthesis details
Pore size, Å:
500
Typical loading, umol/g:
50-80
Coupling conditions:
standard, ultramild phosphoramidites
Cleavage conditions:
2 h / rt, 30% aq. ammonia (ultramild protective groups)
Sphingolipids are amides formed from sphingosin, natural hydrophobic aminodiol, and various carboxylic acids.
This synthetic fluorescent lipid is constructed from sphingosin and BDP TR, a bright fluorescent hydrophobic dye emitting in red region of the spectrum. The lipid is incorporated into membranes of Golgi apparatus and allows to visualize them using confocal microscopy.
BDP dyes are well suited for microscopy of lipids due to their excellent photostability and inherent hydrophobicity.
This sphingosine derivative, BDP TR ceramide, is a molecule designed to visualize Golgi apparatus of live cells under confocal microscopy. The dye emits in orange region.
BDP FL is a green emitting fluorescent dye that is bright, moderately hydrophobic, and highly photostable. Therefore, it is ideally suitable for the study of lipides by means of microscopy.
This conjugate with sphingosine – BDP FL ceramide – is a derivative that is used for the visualization of Golgi apparatus membranes in fluorescent confocal microscopy.
lumiprobe,AF647 carboxylic acid,Reactive dyes,Carboxylic acids, good in DMSO, DMF, water,Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
AF647 NHS ester is a bright, far-red fluorescent dye, used for cellular visualisation and labeling of antibodies, peptides, proteins, modified oligonucleotides, and other amine-containing molecules.
Due to the lack of significant self-quenching AF647 dye molecules can be conjugated to proteins at high molar ratios, which resulting in sensitive detection of low-abundance biomolecules potential.
AF647 NHS ester dye possesses high water-solubility, high fluorescence quantum yield, it is pH-insensitive over a wide molar range. Widely applied for imaging and flow cytometry.
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
标签:ROX (Rhodamine X, Rhodamine 101) NHS esters Microscopy Protein labeling
货号
规格
价格
货期
12220
1 mg
110.00$
in stock
22220
5 mg
210.00$
in stock
42220
25 mg
410.00$
in stock
52220
50 mg
695.00$
in stock
62220
100 mg
1190.00$
in stock
ROX (rhodamine X aka R101) is a highly fluorescent, and photostable rhodamine dye for various applications. ROX labeled oligonucleotide probes are often used in qPCR, and qPCR instruments have ROX channel.
ROX(罗丹明X又名R101)是一种高荧光、耐光的罗丹明染料,适用于各种应用。ROX标记的寡核苷酸探针通常用于qPCR,qPCR仪器具有ROX通道。
This is reactive dye for the labeling of amino-groups in peptides, proteins, and amino-oligonucleotides. Pure single isomer.
这是一种活性染料,用于标记肽、蛋白质和氨基寡核苷酸中的氨基。纯单一异构体。
Absorption and emission spectra of ROX dye
ROX NHS ester, 6- isomer相关产品
Pico488 dsDNA quantification reagent, 200x solution in DMSO
Pico488 is a dye for precise fluorescence based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.
Pico488是一种染料,用于在存在RNA、单链DNA和其他杂质的情况下对双链DNA进行精确的荧光定量。该染料在荧光素通道中表现出明亮的荧光,在低pg/mL到高ng/mL范围内对DNA浓度提供线性响应。
Biotin-PEG3-Azide
Biotin azide is a reagent for click chemistry labeling with biotin, a well-known affinity probe.
叠氮生物素是一种用生物素(一种著名的亲和探针)进行点击化学标记的试剂。
DusQ1 CPG 500
Controlled pore glass (CPG) 500 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.
控制孔玻璃(CPG)500固体载体,用于合成带有3′-末端DusQ1猝灭剂的寡核苷酸。与标准去块条件兼容。
General properties
Appearance:
dark crimson powder
Mass spec M+ increment:
516.2
Molecular weight:
631.67
CAS number:
117491-83-5; 1890922-83-4; 216699-36-4
Molecular formula:
C37H33N3O7
Solubility:
good in polar organic solvents (DMF, DMSO), low in water
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.