标签： HR2-701

Hampton蛋白结晶试剂盒NDSB-201/HR2-701

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Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-201

NDSB-201

NDSB-201
CAT NO

HR2-701

NAME

NDSB-201

DESCRIPTION

5 grams

PRICE

$43.00

支持材料

NDSB-201 HR2-701 NDSB-201 User GuideNDSB-201 HR2-701 NDSB-201 SDS

应用

  • Crystallization grade NDSB-201 for formulating screens or for optimization
  • 结晶级 NDSB-201 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

NDSB-201
Synonyms: 3-(1-Pyridino)-1-propane sulfonate
C8H11NO3S
Mr 201.26
CAS Number [15471-17-7]
EC Number 239-491-3
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

参考

1. Vuillard, L., et al, J. Biochem., 305, 337 (1995).

2. Goldberg, M.E., et al, Folding and Design, 1, 21 (1996).

3. Vuillard et al. J.Cryst Growth (1996) vol 168 pp 150-154.