标签： His Active

ProSpec-EGF Mouse

  • Description

  • EGF Mouse

  • Epidermal Growth Factor Mouse
  • CYT-554

Catalogue number

CYT-554

Synonyms

Urogastrone, URG, EGF.

Introduction

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Description

Epidermal Growth Factor Mouse purified from submaxillary gland is a single, glycosylated, polypeptide chain having a molecular mass of 6.1 kDa.
The EGF is purified by proprietary chromatographic techniques.

Source

Mouse Submaxillary Gland.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a concentrated solution containing 0.01M sodium acetate buffer.

Solubility

It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Epidermal Growth Factor Mouse although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Biological Activity

The biological activity is measured in a proliferation assay using BALB/MK cells.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-EGF Mouse Protein

  • Description

  • EGF Mouse Protein

  • Epidermal Growth Factor Mouse Recombinant
  • CYT-326

Catalogue number

CYT-326

Synonyms

Urogastrone, URG, EGF.

Introduction

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Description

Epidermal Growth Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids including 3 intramolecular disulfide-bonds and having a molecular mass of 6 kDa.
The EGF is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized with no additives.

Solubility

It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR.

Biological Activity

The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 0.1ng/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-EGF Mouse, Biotin

  • Description

  • EGF Mouse, Biotin

  • Epidermal Growth Factor Mouse Recombinant, Biotin
  • CYT-841

Catalogue number

CYT-841

Synonyms

Urogastrone, URG, EGF.

Introduction

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Description

EGF Mouse Recombinant, Biotin produced in E.Coli is a non-glycosylated polypeptide chain containing 61 amino acids and having a total molecular mass of 7.0kDa. This version of EGF has a N terminal leader sequence hosting a biotin conjugation. There are 0.5 biotins for each EGF protein. 

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The protein solution contains sterile PBS.

Stability

Should be stored at 4°C.
Please do not freeze.

Purity

Greater than 95.0% as determined by analysis by -PAGE.

Amino acid sequence

MKKIDDDKNS YPGCPSSYDG YCLNGGVCMH IESLDSYTCN CVIGYSGDRC QTRDLEWWEL R. 

Biological Activity

The ED50 as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells is 0.14-0.2ng/ml, corresponding to a specific activity of 7.1×106units/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-EGF Mouse, His

  • Description

  • EGF Mouse, His

  • Epidermal Growth Factor Mouse Recombinant, His Tag
  • CYT-138

Catalogue number

CYT-138

Synonyms

Urogastrone, URG, EGF.

Introduction

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Description

EGF mouse Recombinant produced in E. coli is a single polypeptide chain containing 77 amino acids and having a molecular mass of 8.6kDa.
EGF is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

E.coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The EGF solution contains 20mM Tris-HCl buffer , 100mM NaCl, 2mM DTT and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMNSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-EGF Mouse, His Active

  • Description

  • EGF Mouse, His Active

  • Epidermal Growth Factor, His Active Mouse Recombinant
  • CYT-1054

Catalogue number

CYT-1054

Synonyms

AI790464, Pro-epidermal growth factor, URG.

Introduction

Pro-Epidermal Growth Factor Isoform 1 or EGF, is a globular peptide which includes three intra molecular disulfide bonds. This protein acts as a growth factor that mediates the growth and proliferation of different epithelial & epidermal cells. Among other processes that EGF is part of are inhibition of gastric secretion and wound healing. EGF is a ligand for class I tyrosine kinase receptor .

Description

EGF Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 77 amino acids and having a molecular mass of 8.6kDa.EGF is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

EGF protein solution contains 10% glycerol, 20mM Tris-HCl , 0.1M NaCl & 2mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Biological Activity

Measured in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 for this effect less or equal to 1ng/ml.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMNSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-IFNG Human, His Active/CYT-1053

ProSpec-IFNG Human, His Active,ProSpec CYT-1053,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,IFNG protein solution containing Phosphate buffered saline and 20% glycerol.

IFNG Human, His Active

Interferon-gamma Human Recombinant, His Tag Active
CYT-1053
ProSpec-IFNG Human, His Active/CYT-1053 2µg
ProSpec-IFNG Human, His Active/CYT-1053 10µg
ProSpec-IFNG Human, His Active/CYT-1053 1mg

Catalogue number

CYT-1053

Synonyms

Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.

Introduction

IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

由特定抗原或有丝分裂原激活的淋巴细胞产生的 IFN-γ。
IFN-γ除具有抗病毒活性外，还具有重要的免疫调节功能，是巨噬细胞的强效激活剂，对转化细胞具有抗增殖作用，并能增强I型干扰素的抗病毒和抗肿瘤作用。

Description

IFNG Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 159 amino acids and having a molecular mass of 18.4kDa. IFNG is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 IFNG Human Recombinant 是一个单一的、非糖基化的多肽链，包含 159 个氨基酸，分子量为 18.4kDa。 IFNG 在 N 末端与 21 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless liquid.

Formulation

IFNG protein solution containing Phosphate buffered saline and 20% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Biological Activity

Measured in a cytotoxicity assay using WiDr cells. The ED50 for this effect is ≤ 5 ng/ml.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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