标签： EdU (5-ethynyl-2′-deoxyuridine)

EdU (5-ethynyl-2′-deoxyuridine)

EdU (5-ethynyl-2′-deoxyuridine),EdU（乙炔基脱氧尿苷）是一种核苷，是胸苷的类似物，带有一个乙炔基。 这种核苷充当细胞 DNA 复制机器的底物。 然后，可以通过点击化学反应与各种染料叠氮化物形成含有乙炔基的 DNA。 荧光 DNA 可以通过显微镜检测，或者细胞可以通过 FACS 分选。

  • Products
  • Click chemistry
  • Non-fluorescent alkynes
  • EdU (5-ethynyl-2′-deoxyuridine)

EdU (5-ethynyl-2′-deoxyuridine)

货号 规格 价格 货期
10540 100 mg 120.00$ in stock
20540 250 mg 195.00$ in stock
30540 500 mg 345.00$ in stock
40540 1 g 590.00$ in stock

EdU (ethynyldeoxyuridine) is a nucleoside, an analog of thymidine, carrying an ethynyl group. This nucleoside behaves as a substrate for cellular DNA replication machinery. Then, DNA containing ethynyl groups can be developed by Click chemistry reaction with various dye azides. Fluorescent DNA can be detected by microscopy, or cells can be sorted by FACS.

EdU（乙炔基脱氧尿苷）是一种核苷，是胸苷的类似物，带有一个乙炔基。 这种核苷充当细胞 DNA 复制机器的底物。 然后，可以通过点击化学反应与各种染料叠氮化物形成含有乙炔基的 DNA。 荧光 DNA 可以通过显微镜检测，或者细胞可以通过 FACS 分选。

EdU (5-ethynyl-2′-deoxyuridine)相关产品

Sulfo-Cyanine5 alkyne

Sulfo-Cyanine5 is a water-soluble Cyanine5 dye alkyne for Click chemistry.

Sulfo-Cy5 是一种用于 Click 化学的水溶性 Cy5 染料炔烃。

THPTA ligand

THPTA is a water soluble ligand for Cu(I) catalyzed Click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.

THPTA 是一种用于 Cu(I) 催化点击化学的水溶性配体。 配体将铜稳定在其 Cu(I) 氧化态。 由于高水溶性，与该配体的反应不需要有机助溶剂。

Sulfo-Cyanine5.5 azid

Sulfo-Cyanine5.5 azide is a derivative of water soluble far red emitting fluorescent cyanine dye. The azide group can be conjugated by either copper-catalyzed or copper free Click chemistry. The reagent possesses high aqueous solubility and hydrophilicity, and recommended for the labeling of biomolecules in aqueous environment.

Sulfo-Cy5.5 azide 是水溶性远红荧光花青染料的衍生物。 叠氮基可以通过铜催化或无铜点击化学进行缀合。 该试剂具有高水溶性和亲水性，推荐用于水环境中生物分子的标记。

General properties

Appearance: light beige to light brown solid浅米色至浅棕色固体
Molecular weight: 252.22
CAS number: 61135-33-9
Molecular formula: C11H12N2O5
Solubility: good in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。
MSDS: Download
Product specifications

相关文献：

  1. Wells, C.I.; Al-Ali, H.; Andrews, D.M.; Asquith, C.R.M.; Axtman, A.D.; Chung, M.; Dikic, I.; Ebner, D.; Elkins, J.M.; Ettmayer, P.; Fischer, C.; Frederiksen, M.; Gray, N.S.; Hatch, S.; Knapp, S.; Lee, S.; Lücking, U.; Michaelides, M.; Mills, C.E.; Müller, S.; Owen, D.; Picado, A.; Ramadan, K.; Saikatendu, K.S.; Schröder, M.; Stolz, A.; Tellechea, M.; Treiber, D.K.; Turunen, B.J.; Vilar, S.; Wang, J.; Zuercher, W.J.; Willson, T.M.; Drewry, D.H. The Kinase Chemogenomic Set (KCGS): An open science resource for kinase vulnerability identification. bioRxiv, preprint. doi: 10.1101/2019.12.22.886523
  2. Lyu, X.; Lei, K.-H.; Biak Sang, P.; Shiva, O.; Chastain, M.; Chi, P.; Chai, W. Human CST complex protects stalled replication forks by directly blocking MRE11 degradation of nascent-strand DNA. EMBO Journal, 2021, 40(2), e103654. doi: 10.15252/embj.2019103654
  3. Irwin, N.A.T.; Pittis, A.A.; Mathur, V.; Howe, L.J.; Keeling, P.J.; Lynn, D.H.; Bourland, W.A. The Function and Evolution of Motile DNA Replication Systems in Ciliates. Current Biology, 2021, 31(1), 66–76.e6. doi: 10.1016/j.cub.2020.09.077
  4. Wang, Z.; Cui, M.; Shah, A.M.; Tan, W.; Liu, N.; Bassel-Duby, R.; Olson, E.N. Cell-Type-Specific Gene Regulatory Networks Underlying Murine Neonatal Heart Regeneration at Single-Cell Resolution. Cell Reports, 2020, 33(10), 108472. doi: 10.1016/j.celrep.2020.108472