- Products
- DNA
- CPG solid supports
- DusQ2 CPG 500
DusQ2 CPG 500
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 12960 | 100 mg | – | 7 days |
| 32960 | 500 mg | 530.00$ | in stock |
| 42960 | 1 g | 990.00$ | in stock |
| 52960 | 5 g | please inquire | in stock |
DusQ2 is a fluorescence quencher with absorption within the range of 560 to 670 nm. It is ideal for effective FRET quenching of fluorophores with emission in this range. The quencher is also used in hybridization probes with static and combined quenching. Its quenching effectiveness does not depend very much on overlapping of fluorophore and quencher spectra, thus allowing for effective quenching of the broad spectrum of fluorophores, including those with emission in the red and far-red part of the spectrum. Thus, DusQ2 can be used with such fluorophores (including but not limited to) as Cyanine3, TAMRA, ROX, Cyanine3.5, Quasar® 570, Pulsar® 650, Cyanine5, Quasar 670, Cyanine5.5, and Quasar 705.
该支持旨在用于自动合成具有 3′-末端 DusQ2 淬灭剂的寡核苷酸。 推荐 500 Å 的孔径用于合成长度不超过 50 个碱基的寡核苷酸。
DusQ2 是一种荧光猝灭剂，吸收范围为 560 至 670 nm。 它非常适合在此范围内发射荧光团的有效 FRET 猝灭。 猝灭剂还用于具有静态和组合猝灭的杂交探针。 其猝灭效果不太依赖于荧光团和猝灭剂光谱的重叠，因此可以有效猝灭广谱荧光团，包括在光谱的红色和远红色部分发射的荧光团。 因此，DusQ2 可以与诸如 Cyanine3、TAMRA、ROX、Cyanine3.5、Quasar® 570、Pulsar® 650、Cyanine5、Quasar 670、Cyanine5.5 和 Quasar 705 的荧光团（包括但不限于）一起使用。
Usage 用法
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
偶联：与正常核碱基相同的标准条件。
脱保护：在室温下使用浓氨水 2 小时或在 65°C 下使用 AMA 混合物、浓氨水/40% 甲胺 (1:1) 脱保护 10 分钟。 脱保护条件取决于寡核苷酸组成和核碱基保护基团，以及其他修饰（如果存在）。
Absorption spectrum of DusQ2
DusQ2 CPG 500相关产品
TFA-Amino modifier CPG 500
TFA-amino modifier CPG for oligo synthesis. 500 Å, high load controlled pore glass.
用于寡核苷酸合成的 TFA-氨基改性剂 CPG。 500 Å，高负载可控孔玻璃。
DusQ2 CPG 1000
Controlled pore glass (CPG) 1000 modified support for the synthesis of oligonucleotides with DusQ2 quencher at the 3’ end. Compatible with standard deblocking conditions.
可控孔玻璃 (CPG) 1000 改良后的 3′ 末端带有 DusQ2 淬灭剂的寡核苷酸合成支持。 与标准解块条件兼容。
FAM Phosphoramidite, 6-Isomer
Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.
用于合成修饰寡核苷酸的单一异构体 6-FAM 亚磷酰胺。
General properties
| Appearance: | dark blue beads |
| Quality control: | NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| ε, L⋅mol−1⋅cm−1: | 34000 |
| CF260: | 0.31 |
| CF280: | 0.26 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 70−80 |