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• Cyanine5 NHS ester

Cyanine5 NHS ester

During the last years, Cyanine5 (an analog of Cy5^®) has become an incredibly popular label in life science research and diagnostics. The fluorophore has its emission maximum in the red region, where many CCD detectors exhibit maximum sensitivity, and biological objects show low background. The dye color is very intense, therefore quantities as small as 1 nmol can be detected in gel electrophoresis by naked eye.

This Cyanine5 NHS ester (analog to Cy5^® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties.

Cyanine5 fluorophore is compatible with various instrumentation including many fluorescent microscopes, imagers, scanners, and fluorescence readers. A number of various Cyanine5 analogs exist – Cyanine5 NHS ester can replace activated esters of Cy5® and DyLight 649.

在过去的几年里，Cyanine5（Cy5® 的类似物）已成为生命科学研究和诊断领域非常受欢迎的标签。荧光团在红色区域具有最大发射，许多 CCD 检测器在该区域表现出最大灵敏度，而生物物体则表现出低背景。染料颜色非常强烈，因此在凝胶电泳中肉眼可以检测到小至 1 nmol 的量。

这种 Cyanine5 NHS 酯（类似于 Cy5® NHS 酯）是一种活性染料，用于标记肽、蛋白质和寡核苷酸中的氨基。这种染料需要少量有机助溶剂（如 DMF 或 DMSO）用于标记反应（请参阅我们推荐的方案了解更多详细信息）。该试剂非常适合用于标记可溶性蛋白质以及各种肽和寡核苷酸的极具成本效益的方法。该试剂在有机溶剂中也适用于小分子标记。对于更复杂的目标，例如易于降解的蛋白质，当不希望使用 DMF 或 DMSO 时，请考虑使用不需要任何助溶剂且具有非常相似荧光特性的水溶性磺基花青 5 NHS 酯。

Cyanine5 荧光团与各种仪器兼容，包括许多荧光显微镜、成像仪、扫描仪和荧光阅读器。存在许多不同的 Cyanine5 类似物 – Cyanine5 NHS 酯可以替代 Cy5® 和 DyLight 649 的活化酯。