标签： Cyanine5

Cy5 phosphoramidite

Cy5 phosphoramidite,Cy5 是 qPCR 中使用的染料之一。 在许多 qPCR 机器上都可以找到相应的过滤器集。

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  • Cyanine5 phosphoramidite

Cyanine5 phosphoramidite

标签：Cyanine5, Phosphoramidite
货号 规格 价格 货期
1740-100mg 100 mg – in stock
1740-250mg 250 mg 290.00$ 10 days
1740-1g 1 g 950.00$ in stock
1740-10g 10 g 6750.00$ 10 days

Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.

This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5′-end, the most typical location of fluorophore. This is a terminating, non-nucleoside reagent.

Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement – a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases stability of the reagent, maintaining efficient coupling over longer time.

Cy5 是 qPCR 中使用的染料之一。 在许多 qPCR 机器上都可以找到相应的过滤器集。

这种反应性衍生物可用于合成在 5′ 端具有花青 5 的 qPCR 探针，这是荧光团最典型的位置。 这是一种终止性非核苷试剂。

该试剂中的酰胺基与二级氧结合。 这为 Arbuzov 重排提供了额外的稳定性 – 这种反应会导致寡核苷酸合成器中溶液中的亚酰胺劣化。 这种分子设计增加了试剂的稳定性，在更长的时间内保持有效的耦合。

Cyanine5 phosphoramidite Cyanine5 phosphoramidite Cyanine5 phosphoramidite Cyanine5 phosphoramidite

Absorption and emission spectra of Cyanine5

Cyanine5 phosphoramidite

Cy5 phosphoramidite相关产品

FAM Phosphoramidite, 6-Isomer

Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.

用于合成修饰寡核苷酸的单一异构体 6-FAM 亚磷酰胺。

Cyanine3 phosphoramidite 5′

Phosphoramidite for the synthesis of 5’-cyanine3-labeled oligonucleotides.

用于合成 5′-菁 3 标记寡核苷酸的亚磷酰胺。

Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

Catalyst for bioconjugate synthesis by Click Chemistry.

Click Chemistry 用于生物共轭合成的催化剂。

General properties

Appearance: dark colored solid
Molecular weight: 816.49
Molecular formula: C47H67N5ClO3P
Quality control: NMR 1H, 31P, HPLC-MS (80%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended

推荐 6 分钟耦合时间
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible

建议在 +4°C 或超温和保护基团下放置 48 小时； 24 小时在 rt 可能

Cyanine5 DBCO

Cyanine5 DBCO,一种具有 DBCO（二苯并环辛炔，也称为 ADIBO，偶氮二苯并环辛炔）基团的 Cyanine5 发红光荧光团的衍生物，用于无铜点击化学。

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  • Cyanine5 DBCO

Cyanine5 DBCO

标签：Cyanine5, Copper free Click chemistry, Cycloalkyne,Cy5
货号 规格 价格 货期
A30F0 1 mg 110.00$ in stock
B30F0 5 mg 210.00$ in stock
C30F0 10 mg 310.00$ in stock
D30F0 25 mg 410.00$ in stock
E30F0 50 mg 695.00$ in stock
F30F0 100 mg 1190.00$ in stock

A derivative of Cyanine5 red-emitting fluorophore possessing DBCO (dibenzocyclooctyne, also known as ADIBO, azodibenzocyclooctyne) group for copper free Click chemistry.

Strained cycloalkynes, such as cyclooctynes, react with azides very rapidly in the absence of copper catalyst in a strain promoted alkyne azide cycloaddition (spAAC). This reaction is very fast, mild, and biocompatible.

Compared to other cycloalkynes, DBCO provide among the fastest reaction kinetics, still possessing good stability.

一种具有 DBCO（二苯并环辛炔，也称为 ADIBO，偶氮二苯并环辛炔）基团的 Cyanine5 发红光荧光团的衍生物，用于无铜点击化学。

在不存在铜催化剂的情况下，在应变促进的炔烃叠氮化物环加成 (spAAC) 中，应变的环炔烃，例如环辛炔，与叠氮化物反应非常迅速。 该反应非常快速、温和且具有生物相容性。

与其他环炔烃相比，DBCO 提供了最快的反应动力学，但仍具有良好的稳定性。

Absorption and emission spectra of Cyanine5

Cyanine5 DBCO

Cyanine5 DBCO相关产品

Cyanine7.5 NHS ester

Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5.

NIR荧光染料Cyanine7.5的胺反应性NHS酯。

Cyanine5.5 amine

Amino derivative of Cyanine5.5, a far red emitting fluorescent cyanine dye.

Cyanine5.5 的氨基衍生物，一种发射远红色荧光的花青染料。

Sulfo-Cyanine5 tetrazine

Water soluble Cyanine dye tetrazine derivative for TCO ligation with trans-cyclooctenes and other strained olefines.

水溶性花青染料四嗪衍生物，用于与反式环辛烯和其他应变烯烃的 TCO 连接。

General properties

Appearance: dark blue solid 深蓝色固体
Mass spec M+ increment: 928.4
Molecular weight: 929.03
Molecular formula: C53H59N4F6O2P
Solubility: good in DMF, DMSO, chlorinated organic solvents, practically insoluble in water (<1 uM, < 1 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

相关文献：

  1. Merlo, R.; Caprioglio, D.; Cillo, M.; Valenti, A.; Mattossovich, R.; Morrone, C.; Massarotti, A.; Rossi, F.; Miggiano, R.; Leonardi, A.; Minassi, A.; Perugino, G. The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate. Journal of Enzyme Inhibition and Medicinal Chemistry, 2021, 36(1), 85–97. doi: 10.1080/14756366.2020.1841182
  2. Qiu, C.-X.; Bai, X.-F.; Shen, Y.; Zhou, Z.; Pan, L.-Q.; Xu, Y.-C.; Zhao, W.-B.; Chen, S.-Q. Specific Inhibition of Tumor Growth by T Cell Receptor-Drug Conjugates Targeting Intracellular Cancer-Testis Antigen NY-ESO-1/LAGE-1. Bioconjugate Chemistry, 2020, 31(12), 2767–2778. doi: 10.1021/acs.bioconjchem.0c00548
  3. Islam, M.R.; Nguy, C.; Pandit, S.; Lyon, L.A. Design and Synthesis of Core–Shell Microgels with One‐Step Clickable Crosslinked Cores and Ultralow Crosslinked Shells. Macromolecular Chemistry and Physics, 2020, 221(19), 2000156. doi: 10.1002/macp.202000156
  4. Liu, W.; Zhao, W.; Bai, X.; Jin, S.; Li, Y.; Qiu, C.; Pan, L.; Ding, D.; Xu, Y.; Zhou, Z.; Chen, S. High antitumor activity of Sortase A-generated anti-CD20 antibody fragment drug conjugates. European Journal of Pharmaceutical Sciences, 2019, 134, 81–92. doi: 10.1016/j.ejps.2019.04.015
Cy® is a trademark of GE Healthcare.

Cyanine5 amine

Cyanine5 amine,Cy5 amine 是一种含有氨基的活性染料，是 Cy5® 胺的类似物。 该试剂可与多种活性酯和其他亲电试剂偶联。 例如，这种胺可以与 EDC 活化的羧基偶联。

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  • Cyanine5 amine

Cyanine5 amine

标签：Cyanine5,Cy5, Amines
货号 规格 价格 货期
130C0 1 mg 110.00$ in stock
230C0 5 mg 210.00$ in stock
430C0 25 mg 410.00$ in stock
530C0 50 mg 695.00$ in stock
630C0 100 mg 1190.00$ in stock

Cyanine5 amine is a reactive dye which contains amino group, an analog of Cy5® amine. This reagent can be coupled with a variety of activated esters and other electrophilic reagents. For example, this amine can be coupled with EDC-activated carboxylic groups.

This bright and photostable dye is suitable for many different methods of fluorescence detection. Colorful fluorophore can also be easily detected in small quantities (nanomols) by naked human eye.

Cyanine5 amine 是一种含有氨基的活性染料，是 Cy5® 胺的类似物。 该试剂可与多种活性酯和其他亲电试剂偶联。 例如，这种胺可以与 EDC 活化的羧基偶联。

这种明亮且光稳定的染料适用于许多不同的荧光检测方法。 肉眼也可以很容易地检测到少量（纳摩尔）的彩色荧光团。

Cyanine5 amine absorbance and emission spectra

Cyanine5 amine

Cyanine5 amine相关产品

BDP FL azide

BDP FL is a bright and photostable borondipyrromethene dye for fluorescein channel. This is a dye azide for Click Chemistry.

BDP FL 是一种用于荧光素通道的明亮且光稳定的硼二吡咯亚甲基染料。 这是一种用于 Click Chemistry 的染料叠氮化物。

Ascorbic acid

Ascorbic acid for the reduction of Cu(II)-TBTA complex.

抗坏血酸用于还原 Cu(II)-TBTA 配合物。

Pico488 dsDNA quantification reagent, 200x solution in DMSO

Pico488 is a dye for precise fluorescence based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.

Pico488 是一种染料，用于在存在 RNA、ssDNA 和其他杂质的情况下对 dsDNA 进行基于荧光的精确定量。 该染料在荧光素通道中显示出明亮的荧光，在低 pg/mL 至高 ng/mL 范围内对 DNA 浓度提供线性响应。

General properties

Appearance: dark blue powder 深蓝色粉末
Molecular weight: 653.77
CAS number: 1807529-70-9
Molecular formula: C38H54Cl2N4O
IUPAC name: 3H-​Indolium, 2-​[5-​[1-​[6-​[(6-​aminohexyl)​amino]​-​6-​oxohexyl]​-​1,​3-​dihydro-​3,​3-​dimethyl-​2H-​indol-​2-​ylidene]​-​1,​3-​pentadien-​1-​yl]​-​1,​3,​3-​trimethyl-
Solubility: moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

相关文献：

  1. Yin, X.; Ren, J.; Lan, W.; Chen, Y.; Ouyang, M.; Su, H.; Zhang, L.; Zhu, J.; Zhang, C. Microfluidics-assisted optimization of highly adhesive haemostatic hydrogel coating for arterial puncture. Bioactive Materials, in press. doi: 10.1016/j.bioactmat.2021.10.009
  2. Dao, N.V.; Ercole, F.; Li, Y.; Davis, T.P.; Kaminskas, L.M.; Sloan, E.K.; Quinn, J.F.; Whittaker, M.R. Nitroxide-functional PEGylated nanostars arrest cellular oxidative stress and exhibit preferential accumulation in co-cultured breast cancer cells. Journal of Materials Chemistry B, 2021, 9(37), 7805–7820. doi: 10.1039/D1TB00812A
  3. Kántor, I.; Aparaschivei, D.; Todea, A.; Biró, E.; Babos, G.; Szerényi, D.; Kakasi, B.; Péter, F:; Şişu, Eugen and Feczkó, T. Biocatalytic synthesis of poly[ε-caprolactone-co-(12-hydroxystearate)] copolymer for sorafenib nanoformulation useful in drug delivery. Catalysis Today, 2021, 366, 195–201. doi: 10.1016/j.cattod.2020.05.005
  4. Mizuno, H.L.; Anraku, Y.; Sakuma, I.; Akagi, Y. Design of a Photocleavable Drug Binding Platform for a Novel Remotely Controllable Drug Coated Balloon. Journal of Drug Delivery Science and Technology, 2021, 62, 102375. doi: 10.1016/j.jddst.2021.102375
Cy® is a trademark of GE Healthcare.

Cyanine5 carboxylic acid

Cyanine5 carboxylic acid,非活化羧酸，Cy5® 游离羧酸的类似物。 含有Cyanine5荧光团。

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  • Cyanine5 carboxylic acid

Cyanine5 carboxylic acid

标签：Cyanine5,Cy5 Carboxylic acids
货号 规格 价格 货期
13090 1 mg 110.00$ in stock
23090 5 mg 210.00$ in stock
43090 25 mg 410.00$ in stock
53090 50 mg 695.00$ in stock
63090 100 mg 1190.00$ in stock

Non-activated carboxylic acid, an analog of Cy5® free carboxylic acid. Contains Cyanine5 fluorophore.

This dye has limited water solubility, but can be dissolved in mixtures of water with organic phase (DMF, DMSO, alcohols) to obtain useful concentrations of the material in solution. Water-soluble version is available.

This molecule can be considered non-reactive dye for the use in control samples, and for instrument calibration. For coupling with amines and protein labeling, consider using Cyanine5 NHS ester, or water-soluble sulfo-Cyanine5 NHS ester.

非活化羧酸，Cy5® 游离羧酸的类似物。 含有Cyanine5荧光团。

这种染料的水溶性有限，但可以溶解在水与有机相（DMF、DMSO、醇）的混合物中，从而在溶液中获得有用浓度的材料。 提供水溶性版本。

这种分子可以被认为是非反应性染料，用于对照样品和仪器校准。 要与胺和蛋白质标记偶联，请考虑使用 Cyanine5 NHS 酯或水溶性磺基-Cyanine5 NHS 酯。

Cyanine5 absorbance and emission spectra

Cyanine5 carboxylic acid

Cyanine5 carboxylic acid相关产品

Sulfo-Cyanine5 maleimide

Thiol reactive, water soluble cyanine dye for Cy5&reg; channel.

用于 Cy5&reg; 的硫醇活性水溶性花青染料 渠道。

Cyanine5 hydrazide

Cy5 hydrazide is a reactive dye for aldehydes and ketones. It can be used for the labeling of glycoproteins after periodate oxidation of sugar moieties.

Cy5 酰肼是醛和酮的活性染料。 它可用于糖部分高碘酸氧化后的糖蛋白的标记。

dsGreen Gel Staining Solution, 10000×

dsGreen for staining dsDNA in gels.

dsGreen 用于在凝胶中染色 dsDNA。

General properties

Appearance: dark blue powder 深蓝色粉末
Molecular weight: 519.12
CAS number: 1032678-07-1 (chloride), 195867-59-5 (inner salt), 766503-38-2 (without anion)
Molecular formula: C32H39ClN2O2
IUPAC name: 3H-​Indolium, 2-​[5-​[1-​(5-​carboxypentyl)​-​1,​3-​dihydro-​3,​3-​dimethyl-​2H-​indol-​2-​ylidene]​-​1,​3-​pentadien-​1-​yl]​-​1,​3,​3-​trimethyl-​, chloride
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), very poorly soluble in water (0.25 mM, 130 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

相关文献：

  1. Petrov, R.A.; Mefedova, S.R.; Yamansarov, E.Y.; Maklakova, S.Y.; Grishin, D.A.; Lopatukhina, E.V.; Burenina, O.Y.; Lopukhov, A.V.; Kovalev, S.V.; Timchenko, Y.V.; Ondar, E.E.; Ivanenkov, Y.A.; Evteev, S.A.; Vaneev, A.N.; Timoshenko, R.V.; Klyachko, N.L.; Erofeev, A.S.; Gorelkin, P.V.; Beloglazkina, E.K.; Majouga, A.G. New Small-Molecule Glycoconjugates of Docetaxel and GalNAc for Targeted Delivery to Hepatocellular Carcinoma. Molecular Pharmaceutics, 2021, 18(1), 461–468. doi: 10.1021/acs.molpharmaceut.0c00980
  2. Wang, Y.; Goun, A.; Laforge, F.; Quine, Z.; Rabitz, H. A key bidirectional switching issue in optogenetics emulated with laser dyes to illustrate its mitigation using nonlinear optical tools. Applied Physics Letters, 2021, 118(2), 024101. doi: 10.1063/5.0035183
  3. Pfister, J.; Lichius, A.; Summer, D.; Haas, H.; Kanagasundaram, T.; Kopka, K.; Decristoforo, C. Live-cell imaging with Aspergillus fumigatus-specific fluorescent siderophore conjugates. Scientific Reports, 2020, 10, 15519. doi: 10.1038/s41598-020-72452-2
  4. Reshitko, G.S.; Yamansarov, E.Y.; Evteev, S.A.; Lopatukhina, E.V.; Shkil’, D.O.; Saltykova, I.V.; Lopukhov, A.V.; Kovalev, S.V.; Lobov, A.N.; Kislyakov, I.V.; Burenina, O.Y.; Klyachko, N.L.; Garanina, A.S.; Dontsova, O.A.; Ivanenkov, Y.A.; Erofeev, A.S.; Gorelkin, P.V.; Beloglazkina, E.K.; Majouga, A.G. Synthesis and Evaluation of New Trivalent Ligands for Hepatocyte Targeting via the Asialoglycoprotein Receptor. Bioconjugate Chemistry, 2020, 31(5), 1313–1319. doi: 10.1021/acs.bioconjchem.0c00202
Cy® is a trademark of GE Healthcare.

Cyanine5 hydrazide

Cyanine5 hydrazide,Cy5 hydrazide 是一种用于标记醛和酮的活性染料，是 Cy5® 酰肼的类似物。这种染料可以与生物分子中遇到的各种羰基进行平稳且几乎定量的反应。 例子是经受氧化应激的蛋白质、通过高碘酸氧化预活化的糖基化蛋白质（包括抗体）和具有醛部分的寡核苷酸。

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  • Cyanine5 hydrazide

Cyanine5 hydrazide

标签: Cyanine5, Cy5,Hydrazides, Microscopy
货号 规格 价格 货期
13070 1 mg 110.00$ in stock
23070 5 mg 210.00$ in stock
43070 25 mg 410.00$ in stock
53070 50 mg 695.00$ in stock
63070 100 mg 1190.00$ in stock

Cyanine5 hydrazide is a reactive dye for the labeling of aldehydes and ketones, an analog of Cy5® hydrazide.

This dye reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. Examples are proteins subjected to oxidative stress, glycosylated proteins pre-activated by periodate oxidation (including antobodies), and oligonucleotides with aldehyde moieties.

Cyanine5 hydrazide replaces carbonyl-reactive Cy5®, DyLight 649 dyes.

Cy5 hydrazide 是一种用于标记醛和酮的活性染料，是 Cy5® 酰肼的类似物。

这种染料可以与生物分子中遇到的各种羰基进行平稳且几乎定量的反应。 例子是经受氧化应激的蛋白质、通过高碘酸氧化预活化的糖基化蛋白质（包括抗体）和具有醛部分的寡核苷酸。

Cyanine5 酰肼替代羰基反应性 Cy5®、DyLight 649 染料。

Cy5 absorbance and emission spectra

Cyanine5 hydrazide

Cyanine5 hydrazide相关产品

ROX reference dye for qPCR

ROX is a passive reference dye for qPCR amplification. The dye can be used to normalize fluorescence intensity of reporter dye in qPCR.

ROX 是用于 qPCR 扩增的被动参考染料。 该染料可用于标准化 qPCR 中报告染料的荧光强度。

FAM DBCO, 6-isomer

Cyclooctyne derivative of fluorescein (FAM) for copper free Click chemistry reaction with azides. Contains pure 6-isomer of fluorescein.

荧光素 (FAM) 的环辛炔衍生物，用于与叠氮化物的无铜点击化学反应。 含有纯6-异构体的荧光素。

Cyanine5 azide

Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.

Cy5 azide 是一种用 Cyanine5（一种流行的荧光染料）进行 Click Chemistry 标记的试剂。

General properties

Appearance: dark blue powder 深蓝色粉末
Molecular weight: 569.61
CAS number: 1427705-31-4
Molecular formula: C32H42Cl2N4O
Solubility: moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

相关文献：

  1. Lix, K.; Krause, K.D.; Kim, H.; Algar, W.R. Investigation of the Energy Transfer Mechanism Between Semiconducting Polymer Dots and Organic Dyes. Journal of Physical Chemistry C, 2020, 124(31), 17387–17400. doi: 10.1021/acs.jpcc.0c04983
  2. Imlimthan, S.; Correia, A.; Figueiredo, P.; Lintinen, K.; Balasubramanian, V.; Airaksinen, A.J.; Kostiainen, M.A.; Santos, H.A.; Sarparanta, M. Systematic In Vitro Biocompatibility Studies of Multimodal Cellulose Nanocrystal and Lignin Nanoparticles. Journal of Biomedical Materials Research. Part A, 2020, 108(3), 770–783. doi: 10.1002/jbm.a.36856
  3. Wang, J.; Johnson, A.G.; Lapointe, C.P.; Choi, J.; Prabhakar, A.; Chen, D.-H.; Petrov, A.N.; Puglisi, J.D. eIF5B gates the transition from translation initiation to elongation. Nature, 2019, 573, 605–608. doi: 10.1038/s41586-019-1561-0
  4. Boone, C.; Grove, R.; Adamcova, D.; Braga, C.; Adamec, J. Revealing oxidative damage to enzymes of carbohydrate metabolism in yeast: An integration of 2D DIGE, quantitative proteomics and bioinformatics. Proteomics, 2016, 16(13), 1889–1903. doi: 10.1002/pmic.201500546
Cy® is a trademark of Cytiva.

Cyanine5 maleimide

Cyanine5 maleimide,Cyanine5 是 Cy5® 的类似物，Cy5® 是一种常见的荧光团，与显微镜、成像仪和荧光阅读器等各种仪器兼容。

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  • Reactive dyes
  • Dye maleimides
  • Cyanine5 maleimide

Cyanine5 maleimide

标签：Cyanine5,Cy5, Maleimides, Microscopy
货号 规格 价格 货期
13080 1 mg 110.00$ in stock
23080 5 mg 210.00$ in stock
43080 25 mg 410.00$ in stock
53080 50 mg 695.00$ in stock
63080 100 mg 1190.00$ in stock

Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.

Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.

For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.

Cy5 马来酰亚胺是一种单反应性染料，可选择性地与硫醇基团（例如，与肽和蛋白质中的半胱氨酸）偶联，形成标记的偶联物。

Cyanine5 是 Cy5® 的类似物，Cy5® 是一种常见的荧光团，与显微镜、成像仪和荧光阅读器等各种仪器兼容。

对于抗体和敏感蛋白的标记，我们建议使用水溶性磺基-Cyanine5 马来酰亚胺。

Cy5 excitation and emission spectra

Cyanine5 maleimide

Cyanine5 maleimide相关产品

FAM hydrazide, 5-isomer

FAM hydrazide is a carbonyl reactive fluorescein derivative. Pure 5-isomer. Can label oxidized proteins, saccharides, and many small molecules with carbonyl groups.

FAM 酰肼是一种羰基反应性荧光素衍生物。 纯5-异构体。 可以用羰基标记氧化的蛋白质、糖类和许多小分子。

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Sulfo-Cyanine5.5 maleimide

Sulfo-Cyanine5.5 is a water-soluble far red emitting dye. This is a thiol reactive maleimide derivative of sulfo-Cyanine5.5. It is recommended for the labeling of sensitive proteins.

Sulfo-Cyanine5.5 是一种水溶性远红发射染料。 这是 sulfo-Cy5.5 的硫醇反应性马来酰亚胺衍生物。 推荐用于敏感蛋白的标记。

Cyanine3 amine

Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.

Cy3 是一种带有游离氨基的荧光团（氨基染料）。 它可以与 NHS 酯、羧基（活化后）和环氧化物结合。

General properties

Appearance: dark blue powder 深蓝色粉末
Molecular weight: 605.8
CAS number: 1437872-46-2 (without anion)
Molecular formula: C38H45N4O3
IUPAC name: 3H-​Indolium, 2-​[5-​[1-​[6-​[[2-​(2,​5-​dihydro-​2,​5-​dioxo-​1H-​pyrrol-​1-​yl)​ethyl]​amino]​-​6-​oxohexyl]​-​1,​3-​dihydro-​3,​3-​dimethyl-​2H-​indol-​2-​ylidene]​-​1,​3-​pentadien-​1-​yl]​-​1,​3,​3-​trimethyl-
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (31 uM, 23 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

相关文献：

  1. Pishesha, N.; Harmand, T.; Smeding, L.; Ma, W.; Ludwig, L.; Janssen, R.; Islam, A.; Xie, Y.; Fang, T.; McCaul, N.; Pinney, W.; Sugito, H.; Ploegh, H. Single domain antibody-antigen adducts that target Class II MHC induce antigen-specific tolerance. Research Square, preprint. doi: 10.21203/rs.3.rs-192181/v1
  2. Delvaux, N.A.; Mathew, B.; Rice, K.G. Fluorescent Labeling of Plasmid DNA for Gene Delivery: Implications of Dye Hydrophobicity on Labeling Efficiencies and Nanoparticle Size. Analytical Biochemistry, in press. doi: 10.1016/j.ab.2020.113895
  3. Yang, M.; Zhang, L.; Sun, R.; Zhong, W.; Yang, Y.; Chen, C. Streptococcus pyogenes Cas9 displays biased one-dimensional diffusion on dsDNA to search for a target. bioRxiv, preprint. doi: 10.1101/754564
  4. Ast, J.; Arvaniti, A.; Fine, N.H.F.; Nasteska, D.; Ashford, F.B.; Stamataki, Z.M Koszegi, Z.; Bacon, A.; Trapp, S.; Jones, B.J.; Hastoy, B.; Tomas, A.; Reissaus, C.; Linnemann, A.K.; D’Este, E.; Calebiro, D.; Johnsson, K.; Podewin, T.; Broichhagen, J.; Hodson, D.J. LUXendins reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics. bioRxiv, preprint. doi: 10.1101/557132
Cy® is a trademark of GE Healthcare.

Cyanine5 alkyne,Cy5炔烃

Cyanine5 alkyne,Cyanine5 炔烃用于 Click Chemistry，是 Cy5® 炔烃的类似物。 使用该产品，颜色深且光稳定的 Cyanine5 荧光团可以通过与叠氮化物的 Click Chemistry 反应连接到各种分子上。

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  • Reactive dyes
  • Dye alkynes
  • Cyanine5 alkyne

Cyanine5 alkyne

标签：Cyanine5,Cy5, Alkynes, Microscopy
货号 规格 价格 货期
A30B0 1 mg 110.00$ in stock
B30B0 5 mg 210.00$ in stock
C30B0 10 mg 310.00$ in stock
D30B0 25 mg 410.00$ in stock
E30B0 50 mg 695.00$ in stock
F30B0 100 mg 1190.00$ in stock

Cyanine5 alkyne for Click Chemistry, an analog of Cy5® alkyne. With this product, deeply colored, and photostable Cyanine5 fluorophore can be attached to various molecules via Click Chemistry reaction with azides.

This alkyne is non-water soluble, but it can be dissolved in DMF or DMSO prior to reaction, and added to aqueous reaction mixture. With our labeling protocol, the labeling reaction is very efficient, and high-yielding.

Various substrates bearing azides can be used for the labeling, including azido-labeled biomolecules, polymers, and solid surfaces.

Cyanine5 炔烃用于 Click Chemistry，是 Cy5® 炔烃的类似物。 使用该产品，颜色深且光稳定的 Cyanine5 荧光团可以通过与叠氮化物的 Click Chemistry 反应连接到各种分子上。

这种炔烃不溶于水，但它可以在反应前溶解在 DMF 或 DMSO 中，然后加入到含水反应混合物中。 使用我们的标记方案，标记反应非常有效且产量高。

带有叠氮化物的各种基材可用于标记，包括叠氮标记的生物分子、聚合物和固体表面。

Cyanine5 absorbance and emission spectra

Cyanine5 alkyne

Cyanine5 alkyne

Sulfo-Cyanine3 alkyne

A water soluble dye alkyne for copper-catalyzed Click Chemistry. The fluorophore Sulfo-Cyanine3 is a bright and photostable dye for the Cy3® channel.

一种用于铜催化点击化学的水溶性染料炔烃。 荧光团 Sulfo-Cyanine3 是一种用于 Cy3® 通道的明亮且光稳定的染料。

Pico488 dsDNA quantification reagent, 200x solution in DMSO

Pico488 is a dye for precise fluorescence based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.

Pico488 是一种染料，用于在存在 RNA、ssDNA 和其他杂质的情况下对 dsDNA 进行基于荧光的精确定量。 该染料在荧光素通道中显示出明亮的荧光，在低 pg/mL 至高 ng/mL 范围内对 DNA 浓度提供线性响应。

Ascorbic acid

Ascorbic acid for the reduction of Cu(II)-TBTA complex.

抗坏血酸用于还原 Cu(II)-TBTA 配合物。

General properties

Appearance: dark blue powder 深蓝色粉末
Molecular weight: 556.18
CAS number: 1223357-57-0
Molecular formula: C35H42ClN3O
Solubility: good in dichloromethane, DMF, DMSO, alcohols, very poorly soluble in water (200 mg/L = 0.4 mM)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

相关文献：

  1. Liu, M.; Apriceno, A.; Sipin, M.; Scarpa, E.; Rodriguez-Arco, L.; Poma, A.; Marchello, G.; Battaglia, G.; Angioletti-Uberti, S. Combinatorial entropy behaviour leads to range selective binding in ligand-receptor interactions. Nature Communications, 2020, 11, 4836. doi: 10.1038/s41467-020-18603-5
  2. Kocere, A.; Resseguier, J.; Wohlmann, J.; Skjeldal, F.M.; Khan, S.; Speth, M.; Dal, N.-J.K.; Ng, M.Y.W.; Alonso-Rodriguez, N.; Scarpa, E.; Rizzello, L.; Battaglia, G.; Griffiths, G.; Fenaroli, F. Real-time imaging of polymersome nanoparticles in zebrafish embryos engrafted with melanoma cancer cells: Localization, toxicity and treatment analysis. EBioMedicine, 2020, 58, 102902. doi: 10.1016/j.ebiom.2020.102902
  3. Monteiro, P.F.; Gulfam, M.; Monteiro, C.J.; Travanut, A.; Abelha, T.F.; Pearce, A.K.; Jerôme, C.; Grabowska, A.M.; Clarke, P.A.; Collins, H.M.; Heery, D.M.; Gershkovich, P.; Alexander, C. Synthesis of micellar-like terpolymer nanoparticles with reductively-cleavable cross-links and evaluation of efficacy in 2D and 3D models of triple negative breast cancer. Journal of Controlled Release, 2020, 323, 549–564. doi: 10.1016/j.jconrel.2020.04.049
  4. Zivanovic, J.; Kouroussis, E.; Kohl, J.B.; Adhikari, B.; Bursac, B.; Schott-Roux, S.; Petrovic, D.; Miljkovic, J.L.; Thomas-Lopez, D.; Jung, Y.; Miler, M.; Mitchell, S.; Milosevic, V.; Gomes, J.E.; Benhar, M.; Gonzales-Zorn, B.; Ivanovic-Burmazovic, I.; Torregrossa, R:; Mitchell, J.R.; Whiteman, M.; Schwarz, G.; Snyder, S.H.; Paul, B.D.; Carroll, K.S.; Filipovic, M.R. Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration. Cell Metabolism, 2019, 30(6), 1152–1170.e13. doi: 10.1016/j.cmet.2019.10.007
Cy® is a trademark of GE Healthcare.

Cy5 azide,Cy5 叠氮化物

Cy5 azide,Cy5 叠氮化物,用于 Click Chemistry 的 Cy5 叠氮化物标记试剂，以 10 mM DMSO 溶液和固体形式提供。这种叠氮化物可溶于有机溶剂（例如 DMSO、DMF），因此标记反应应使用少量有机助溶剂进行。 这种叠氮化物可用于在水与有机溶剂的混合物中标记炔烃修饰的生物分子。 DMSO 中的溶液可用于生物偶联。 该试剂的水溶性磺化版本也可用。

  • Products
  • Reactive dyes
  • Dye azides
  • Cyanine5 azide

Cyanine5 azide

标签：Cyanine5, Cy5,Azides, Microscopy
货号 规格 价格 货期
13030 100 uL, 10 mM/DMSO 110.00$ in stock
33030 500 uL, 10 mM/DMSO 210.00$ in stock
43030 1 mL, 10 mM/DMSO 410.00$ in stock
A3030 1 mg 110.00$ in stock
B3030 5 mg 210.00$ in stock
C3030 10 mg 310.00$ in stock
D3030 25 mg 410.00$ in stock
E3030 50 mg 695.00$ in stock
F3030 100 mg 1190.00$ in stock

Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO, and in solid form.

This azide is soluble in organic solvents (e.g. DMSO, DMF), therefore the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.

Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores which is compatible with various instruments. Cyanine5 can also be used as a replacement for DyLight® 649.

用于 Click Chemistry 的 Cyanine5 叠氮化物标记试剂，以 10 mM DMSO 溶液和固体形式提供。

这种叠氮化物可溶于有机溶剂（例如 DMSO、DMF），因此标记反应应使用少量有机助溶剂进行。 这种叠氮化物可用于在水与有机溶剂的混合物中标记炔烃修饰的生物分子。 DMSO 中的溶液可用于生物偶联。 该试剂的水溶性磺化版本也可用。

Cyanine5 是 Cy5® 的类似物，Cy5® 是最常用的荧光团之一，可与各种仪器兼容。 Cyanine5 也可用作 DyLight® 649 的替代品。

Cyanine5 absorbance and emission spectra

Cyanine5 azide

Cy5 azide,Cy5 叠氮化物相关产品

DBCO NHS ester

Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain promoted copper free Click chemistry (spAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.

用于应变促进无铜点击化学 (spAAC) 的偶氮二苯并环辛炔 (DBCO, ADIBO) 试剂。 该试剂含有 NHS 酯功能，用于将环辛炔连接到各种分子上。

BDP 581/591 alkyne

BDP 581/591 is a borondipyrromethene fluorophore which is useful for fluorescence polarization assays, microscopy, and two photon experiments. This alkyne is a derivative for copper-catalyzed Click chemistry.

BDP 581/591 是一种硼二吡咯亚甲基荧光团，可用于荧光偏振测定、显微镜检查和两个光子实验。 该炔烃是铜催化点击化学的衍生物。

Ascorbic acid

Ascorbic acid for the reduction of Cu(II)-TBTA complex.

抗坏血酸用于还原 Cu(II)-TBTA 配合物。

General properties

Appearance: dark blue powder / solution
Molecular weight: 601.22
CAS number: 1267539-32-1 (chloride)
Molecular formula: C35H45ClN6O
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

相关文献：

  1. Thauvin, C.; Maudens, P.; Allémann, E. Microwave-assisted synthesis of self-assembling bi-functionalizable amphiphilic diblock copolymers. Journal of Drug Delivery Science and Technology, 2021, 61, 102255. doi: 10.1016/j.jddst.2020.102255
  2. Boersma, S.; Rabouw, H.H.; Bruurs, L.J.M.; Pavlovič, T.; van Vliet, A.L.W.; Beumer, J.; Clevers, H.; van Kuppeveld, F.J.M.; Tanenbaum, M.E. Translation and Replication Dynamics of Single RNA Viruses. Cell, 2020, 183(7), 1930–1945.e23. doi: 10.1016/j.cell.2020.10.019
  3. Kim, S.M.; Park, J.; Kim, M.S.; Song, H.; Jo, A.; Park, H.; Kim, T.S.; Choi, S.H.; Park, S.B. Phenotypic Discovery of an Antivirulence Agent against Vibrio vulnificus via Modulation of Quorum-Sensing Regulator SmcR. ACS Infectious Diseases, 2020, 6(11), 3076–3082. doi: 10.1021/acsinfecdis.0c00587
  4. von Döhren, J.; Kuhl, S. A simple method for long-term vital-staining of ciliated epidermal cells in aquatic larvae. Journal of Biological Methods, 2020, 7(2), e132. doi: 10.14440/jbm.2020.320
Cy® is a trademark of Cytiva.