Cyanine3.5 DBCO

Cyanine3.5 DBCO,Cy3.5 是一种明亮且光稳定的荧光团,其光谱特性与 Cy3.5™ 染料相似。 Cyanine3.5 DBCO 荧光最大值在 604 nm 处,在橙红色光谱范围内。

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  • Cyanine3.5 DBCO

Cyanine3.5 DBCO

标签:Cyanine3.5, Cycloalkyne,Cy3.5
货号 规格 价格 货期
A20F0 1 mg 110.00$ in stock
B20F0 5 mg 210.00$ in stock
C20F0 10 mg 310.00$ in stock
D20F0 25 mg 410.00$ in stock
E20F0 50 mg 695.00$ in stock
F20F0 100 mg 1190.00$ in stock

Cyanine3.5 is a bright and photostable fluorophore, which is similar in its spectral characteristics to Cy3.5™ dye. Cyanine3.5 DBCO fluorescence maximum is at 604 nm and in the orange-red spectrum range.

Cyanine3.5 fluorescence is pH insensitive in the range from 4 to 10 and can be detected in the spectrum range with a low level of autofluorescence of biological samples.

Cyanine3.5 DBCO (dibenzocyclooctyne) easily forms conjugates with azide derivatives of biomolecules in copper-free Click Chemistry. The process runs without catalysts or increased temperature, resulting in stable triazoles.

Conjugates of biomolecules with Cyanine3.5 can be used for various microscopy assays, including FRET-microscopy, and in proteomics.

Cyanine3.5 是一种明亮且光稳定的荧光团,其光谱特性与 Cy3.5™ 染料相似。 Cyanine3.5 DBCO 荧光最大值在 604 nm 处,在橙红色光谱范围内。

Cyanine3.5 荧光在 4 到 10 的范围内对 pH 不敏感,并且可以在生物样品的低水平自发荧光的光谱范围内检测到。

Cyanine3.5 DBCO(二苯并环辛炔)在无铜点击化学中很容易与生物分子的叠氮化物衍生物形成共轭物。 该过程在没有催化剂或升高温度的情况下运行,从而产生稳定的三唑。

生物分子与 Cyanine3.5 的偶联物可用于各种显微镜分析,包括 FRET 显微镜和蛋白质组学。

Absorption and emission spectra of Cyanine3.5

Cyanine3.5 DBCO

Cyanine3.5 DBCO相关产品

Cyanine5 DBCO

Cyanine5 dye with dibenzocyclooctyne (DBCO) functional group for copper free Click chemistry.

用于无铜点击化学的具有二苯并环辛炔 (DBCO) 官能团的 Cy5 染料。

Cyanine3 DBCO

Cyanine3 dibenzocyclooctyne (DBCO, or ADIBO) is a cycloalkyne dye derivative for copper free Click chemistry.

Cy3 dibenzocyclooctyne (DBCO, or ADIBO) 是一种用于无铜点击化学的环炔烃染料衍生物。

sulfo-Cyanine5.5 DBCO

Sulfo-Cyanine5.5 DBCO is a far-red, water-soluble reagent for copper-free reaction with azides.

Sulfo-Cy5.5 DBCO 是一种远红色的水溶性试剂,用于与叠氮化物的无铜反应。

General properties

Appearance: dark violet solid 深紫色固体
Molecular weight: 944.95
Molecular formula: C59H61N4BF4O2
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 591
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35
CF260: 0.29
CF280: 0.22
Cy™ is a registered trademark of Cytiva in some countries.

Cyanine3.5 carboxylic acid

Cyanine3.5 carboxylic acid,Cy3.5 游离未活化的单官能羧酸。 可用作参考非活性染料或对照。 Cyanine3.5 是 Cy3.5® 荧光团的类似物。

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  • Cyanine3.5 carboxylic acid

Cyanine3.5 carboxylic acid

标签:Cyanine3.5,Cy3.5, Carboxylic acids
货号 规格 价格 货期
12090 1 mg 110.00$ in stock
22090 5 mg 210.00$ in stock
42090 25 mg 410.00$ in stock
52090 50 mg 695.00$ in stock
62090 100 mg 1190.00$ in stock

Cyanine3.5 free unactivated monofunctional carboxylic acid. Can be used as a reference non-reactive dye or control. Cyanine3.5 is an analog of Cy3.5® fluorophore.

Cy3.5 游离未活化的单官能羧酸。 可用作参考非活性染料或对照。 Cyanine3.5 是 Cy3.5® 荧光团的类似物。

Cyanine3.5 absorbance and emission spectra

Cyanine3.5 carboxylic acid

Cyanine3.5 carboxylic acid相关产品

Sulfo-Cyanine5 azide

Water soluble sulfo-Cyanine5 fluorescent dye azide for Click Chemistry.

用于 Click Chemistry 的水溶性磺基-Cy5 荧光染料叠氮化物。

Cyanine3.5 NHS ester

Cyanine3.5 dye NHS ester derivative.

Cyanine3.5染料NHS酯衍生物。

Cyanine5.5 carboxylic acid

Cy5.5 unactivated free acid cyanine dye.

Cy5.5 未活化的游离酸性花青染料。

General properties

Appearance: dark purple powder 深紫色粉末
Molecular weight: 593.20
CAS number: 1802928-88-6 (chloride), 1144107-79-8 (inner salt), 1802978-82-0 (without anion)
Molecular formula: C38H41ClN2O2
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 591
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35
CF260: 0.29
CF280: 0.22

相关文献:

  1. Kwok, S.J.J.; Choi, M.; Bhayana, B.; Zhang, X.; Ran, C.; Yun, S.-H. Two-photon excited photoconversion of cyanine-based dyes. Scientific Reports, 2016, 6, 23866. doi: 10.1038/srep23866
Cy® is a trademark of GE Healthcare.

Cy3.5 azide ,Cy3.5 染料叠氮化物

Cy3.5 azide ,Cy3.5 染料叠氮化物,Cyanine3.5 标记试剂、固体材料和 10 mM DMSO 溶液,用于 Click Chemistry。可替代 Cy3.5®、DyLight 594。

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  • Cyanine3.5 azide

Cyanine3.5 azide

标签:Cyanine3.5, Cy3.5,Azides
货号 规格 价格 货期
12030 100 uL, 10 mM/DMSO 110.00$ in stock
32030 500 uL, 10 mM/DMSO 210.00$ in stock
42030 1 mL, 10 mM/DMSO 410.00$ in stock
A2030 1 mg 110.00$ in stock
B2030 5 mg 210.00$ in stock
C2030 10 mg 310.00$ in stock
D2030 25 mg 410.00$ in stock
E2030 50 mg 695.00$ in stock
F2030 100 mg 1190.00$ in stock

Cyanine3.5 labeling reagent, solid material and 10 mM solution in DMSO, for Click Chemistry.

Can replace Cy3.5®, DyLight 594.

Cyanine3.5 标记试剂、固体材料和 10 mM DMSO 溶液,用于 Click Chemistry。

可替代 Cy3.5®、DyLight 594。

Cy3.5 absorbance and emission spectra

Cyanine3.5 azide

Cy3.5 azide ,Cy3.5 染料叠氮化物相关产品

PEP azide

PEP (phenylethynylpyrene), polyaromatic hydrocarbon fluorescent dye for Click-Chemistry labeling.

PEP(苯乙炔基芘),用于 Click-Chemistry 标记的多环芳烃荧光染料。

Alkyne Phosphoramidite, 5′-terminal

Alkyne phosphoramidite for the synthesis of oligonucleotides ready for Click chemistry.

用于合成寡核苷酸的炔亚磷酰胺,可用于点击化学。

Coumarin 343 X NHS ester

Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.

香豆素 343 是一种蓝色发射染料,可用作荧光素 (FAM) 的 FRET 供体。 这种结构的特点是香豆素 343 荧光团和官能团之间有一个接头。 它提供了更好的溶解性,以及染料和官能团之间的空间分离。

General properties

Appearance: dark purple powder / solution 深紫色粉末/溶液
Molecular weight: 675.31
Molecular formula: C41H47N6ClO
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 591
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35
CF260: 0.29
CF280: 0.22

相关文献:

  1. Jandl, B.; Sedghiniya, S.; Carstens, A.; Astakhova, K. Peptide–Fluorophore Hydrogel as a Signal Boosting Approach in Rapid Detection of Cancer DNA. ACS Omega, 2019, 4(9), 13889–13895. doi: 10.1021/acsomega.9b01586
  2. Gaitzsch, J.; Delahaye, M.; Poma, A.; Du Prez, F.; Battaglia, G. Comparison of metal free polymer-dye conjugation strategies in protic solvents. Polymer Chemistry, 2016, 7(17), 3046–3055;. doi: 10.1039/c6py00518g
  3. Astakhova, I.K.; Wengel, J. Interfacing Click Chemistry with Automated Oligonucleotide Synthesis for the Preparation of Fluorescent DNA Probes Containing Internal Xanthene and Cyanine Dyes. Chemistry – a European Journal, 2013, 19(3), 1112-1122. doi: 10.1002/chem.201202621
Cy® is a trademark of GE Healthcare.

Cyanine3.5 NHS ester

Cyanine3.5 NHS ester,Cyanine3.5 NHS 酯(Cy3.5® NHS 酯的类似物)是一种活性染料,用于标记肽、蛋白质和寡核苷酸中的氨基。Cyanine3.5 NHS 酯可替代 Cy3.5®、DyLight 594 的 NHS 酯。

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  • Reactive dyes
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  • Cyanine3.5 NHS ester

Cyanine3.5 NHS ester

标签:Cyanine3.5 NHS esters Protein labeling
货号 规格 价格 货期
12020 1 mg 110.00$ in stock
22020 5 mg 210.00$ in stock
42020 25 mg 410.00$ 5 days
52020 50 mg 695.00$ in stock
62020 100 mg 1190.00$ in stock

Cyanine3.5 NHS ester (an analog of Cy3.5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.

Cyanine3.5 NHS ester can replace NHS esters of Cy3.5®, DyLight 594.

Cyanine3.5 NHS 酯(Cy3.5® NHS 酯的类似物)是一种活性染料,用于标记肽、蛋白质和寡核苷酸中的氨基。

Cyanine3.5 NHS 酯可替代 Cy3.5®、DyLight 594 的 NHS 酯。

Cyanine3.5 NHS ester Cyanine3.5 NHS ester Cyanine3.5 NHS ester Cyanine3.5 NHS ester

Cyanine3.5 NHS ester absorbance and emission spectra

Cyanine3.5 NHS ester

Cyanine3.5 NHS ester相关产品

Sulfo-Cyanine5.5 maleimide

Sulfo-Cyanine5.5 is a water-soluble far red emitting dye. This is a thiol reactive maleimide derivative of sulfo-Cyanine5.5. It is recommended for the labeling of sensitive proteins.

Sulfo-Cyanine5.5 是一种水溶性远红发射染料。 这是 sulfo-Cyanine5.5 的硫醇反应性马来酰亚胺衍生物。 推荐用于敏感蛋白的标记。

Cyanine7.5 amine

Near infrared dye Cyanine7.5 with free amino group.

具有游离氨基的近红外染料Cyanine7.5。

ROX azide, 5- isomer

ROX (aka rhodamine X or rhodamine 101) azide for biomolecule labeling through Click Chemistry.

ROX(又名罗丹明 X 或罗丹明 101)叠氮化物,用于通过 Click Chemistry 进行生物分子标记。

General properties

Appearance: dark purple solid
Molecular weight: 741.62
CAS number: 2231670-85-0
Molecular formula: C42H44N3BF4O4
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 591
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35
CF260: 0.29
CF280: 0.22

相关文献:

  1. Lee, S.A.; Biteen, J.S. Spectral Reshaping of Single Dye Molecules Coupled to Single Plasmonic Nanoparticles. Journal of Physical Chemistry Letters, 2019, 10, 5764–5769. doi: 10.1021/acs.jpclett.9b02480
  2. Kravchuk, O.I.; Lyupina, Y.V.; Erokhov, P.A.; Finoshin, A.D.; Adameyko, K.I.; Mishyna, M.Y.; Moiseenko, A.V.; Sokolova, O.S.; Orlova, O.V.; Beljelarskaya, S.N.; Serebryakova, M.V.; Indeykina, M.I.; Bugrova, A.E.; Kononikhin, A.S.; Mikhailov, V.S. Characterization of the 20S proteasome of the lepidopteran, Spodoptera frugiperda. Biochimica et Biophysica Acta – Proteins and Proteomics, 2019, 1867(9), 840–853. doi: 10.1016/j.bbapap.2019.06.010
  3. Wang, C.; Niederstrasser, H.; Douglas, P.M.; Lin, R.; Jaramillo, J.; Li, Y.; Olswald, N.W.; Zhou, A.; McMillan, E.A.; Mendiratta, S.; Wang, Z.; Zhao, T.; Lin, Z.; Luo, M.; Huang, G.; Brekken, R.A.; Posner, B.A.; MacMillan, J.B.; Gao, J.; White, M.A. Small-molecule TFEB pathway agonists that ameliorate metabolic syndrome in mice and extend C. elegans lifespan. Nature Communications, 2017, 8, 2270. doi: 10.1038/s41467-017-02332-3
  4. Massey, M.; Kim, H.; Conroy, E.M.; Algar, W.R. Expanded Quantum Dot-Based Concentric Förster Resonance Energy Transfer: Adding and Characterizing Energy-Transfer Pathways for Triply Multiplexed Biosensing. The Journal of Physical Chemistry C, 2017, 121(24), 13345–13356. doi: 10.1021/acs.jpcc.7b02739