Cy3 azide,Cy3 叠氮化物

Cy3 azide,Cy3 叠氮化物,用于 Click Chemistry 的 Cyanine3 染料叠氮化物,是 Cy3® 叠氮化物的类似物。 Cy3® 是使用最广泛的荧光团之一,可以通过各种荧光计、成像仪和显微镜进行检测。 由于固有的高消光系数,Cyanine3 也很容易通过肉眼在凝胶上和溶液中检测到。 这是一种非磺化染料,需要有机助溶剂(DMF、DMSO 或其他)在水中进行有效标记。 该试剂的水溶性版本也可用。

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Cyanine3 azide

标签:Cyanine3, Cy3,Azides, Microscopy
货号 规格 价格 货期
11030 100 uL, 10 mM/DMSO 110.00$ in stock
31030 500 uL, 10 mM/DMSO 210.00$ in stock
41030 1 mL, 10 mM/DMSO 410.00$ in stock
A1030 1 mg 110.00$ in stock
B1030 5 mg 210.00$ in stock
C1030 10 mg 310.00$ in stock
D1030 25 mg 410.00$ in stock
E1030 50 mg 695.00$ in stock
F1030 100 mg 1190.00$ in stock

Cyanine3 dye azide for Click Chemistry, an analog of Cy3® azide. Cy3® is one of the most broadly used fluorophores which can be detected by various fluorometers, imagers, and microscopes. Due to inherently high extinction coefficient, Cyanine3 is also easily detected by naked eye on gels, and in solution. This is non-sulfonated dye which requires organic co-solvent (DMF, DMSO, or other) for efficient labeling in water. Water-soluble version of this reagent is also available.

Product is available both as solid compound, and as 10 mM solution in DMSO which is ready to use in our recommended protocol.

Cyanine3 fluorescent properties are identical to Cy3®, and similar to DyLight 549.

用于 Click Chemistry 的 Cyanine3 染料叠氮化物,是 Cy3® 叠氮化物的类似物。 Cy3® 是使用最广泛的荧光团之一,可以通过各种荧光计、成像仪和显微镜进行检测。 由于固有的高消光系数,Cyanine3 也很容易通过肉眼在凝胶上和溶液中检测到。 这是一种非磺化染料,需要有机助溶剂(DMF、DMSO 或其他)在水中进行有效标记。 该试剂的水溶性版本也可用。

产品既可以作为固体化合物提供,也可以作为 10 mM DMSO 溶液提供,可在我们推荐的方案中使用。

Cyanine3 荧光特性与 Cy3® 相同,与 DyLight 549 相似。

Cy3 absorbance and emission spectra

Cyanine3 azide

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PEP azide

PEP (phenylethynylpyrene), polyaromatic hydrocarbon fluorescent dye for Click-Chemistry labeling.

PEP(苯乙炔基芘),用于 Click-Chemistry 标记的多环芳烃荧光染料。

Cyanine3 amine

Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.

Cy3 是一种带有游离氨基的荧光团(氨基染料)。 它可以与 NHS 酯、羧基(活化后)和环氧化物结合。

BDP 581/591 alkyne

BDP 581/591 is a borondipyrromethene fluorophore which is useful for fluorescence polarization assays, microscopy, and two photon experiments. This alkyne is a derivative for copper-catalyzed Click chemistry.

BDP 581/591 是一种硼二吡咯亚甲基荧光团,可用于荧光偏振测定、显微镜检查和两个光子实验。 该炔烃是铜催化点击化学的衍生物。

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General properties

Appearance: red powder / solution 红粉/溶液
Molecular weight: 575.19
CAS number: 1167421-28-4 (chloride)
Molecular formula: C33H43N6OCl
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (40 mg/L = 60 uM)
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

相关文献:

  1. Kim, S.M.; Park, J.; Kim, M.S.; Song, H.; Jo, A.; Park, H.; Kim, T.S.; Choi, S.H.; Park, S.B. Phenotypic Discovery of an Antivirulence Agent against Vibrio vulnificus via Modulation of Quorum-Sensing Regulator SmcR. ACS Infectious Diseases, 2020, 6(11), 3076–3082. doi: 10.1021/acsinfecdis.0c00587
  2. Tan, M.S.Y.; Davison, D.; Sanchez, M.I.; Anderson, B.M.; Howell, S.; Snijders, A.; Edgington-Mitchell, L.E.; Deu, E. Novel broad-spectrum activity-based probes to profile malarial cysteine proteases. PLoS One, 2020, 15(1), e0227341. doi: 10.1371/journal.pone.0227341
  3. Saqr, A.; Vakili, M.R.; Huang, Y.-H.; Lai, R.; Lavasanifar, A. Development of Traceable Rituximab-Modified PEO-Polyester Micelles by Postinsertion of PEG-phospholipids for Targeting of B-cell Lymphoma. ACS Omega, 2019, 4(20), 18867–18879. doi: 10.1021/acsomega.9b02910
  4. Morimoto, K.; Cole, K.S.; Kourelis, J.; Witt, C.H.; Brown, D.; Krahn, D.; Stegmann, M.; Kaschani, F.; Kaiser, M.; Burton, J.; Mohammed, S.; Yamaguchi-Shinozaki, K.; Weerapana, E.; van der Hoorn, R.A.L. Triazine Probes Target Ascorbate Peroxidases in Plants. Plant Physiology, 2019, 180(4), 1848–1859. doi: 10.1104/pp.19.00481
Cy® is a trademark of Cytiva.

lumiprobe -Cyanine3B carboxylic acid,2321-1mg

lumiprobe -Cyanine3B carboxylic acid,2321-1mg,Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

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  • Cyanine3B carboxylic acid

Cyanine3B carboxylic acid

Cyanine3, Carboxylic acids, sulfo-Cyanine3,Cy3
Cat. # Quantity Price Lead time
2321-1mg 1 mg 110.00$ 8 days
2321-5mg 5 mg 149.00$ 8 days
2321-25mg 25 mg 690.00$ 8 days
2321-50mg 50 mg 1290.00$ 8 days
2321-100mg 100 mg 2450.00$ 8 days

General properties

Appearance: dark red powder
Molecular weight: 560.66
Molecular formula: C31H32N2O6S
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Cyanine3B carboxylic acid

Cyanine3 phosphoramidite 5′

Cyanine3 phosphoramidite 5′,Cy3 是一种荧光团,广泛用于分子生物学实验,例如寡核苷酸标记和寡核苷酸检测。根据其光谱特性,Cyanine3 是 Cy™3 的类似物,在黄色光谱范围内的荧光最大值为 570 nm。

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Cyanine3 phosphoramidite 5′

标签:Cy3, Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
2771-100mg 100 mg in stock
2771-250mg 250 mg 290.00$ 10 days
2771-1g 1 g 950.00$ in stock
2771-10g 10 g 6750.00$ 21 days

Cyanine3 is a fluorophore that is widely used in molecular biology experiments such as oligonucleotide labeling followed by oligonucleotide detection. By its spectral characteristics, Cyanine3 is an analog of Cy™3 with a fluorescence maximum at 570 nm in the yellow spectrum range.

Cyanine3 phosphoramidite 5’ is used in oligonucleotide synthesis for the production of 5’-cyanine3-labeled oligonucleotides. The reagent is compatible with various oligonucleotide synthesizers.

This phosphoramidite can be used for the synthesis of fluorescence-labeled primers and hybridization probes such as TaqMan and Molecular Beacon. Such labeled probes can be detected in multiplex real-time PCR in the TAMRA channel.

Condensation: 3 min. Use 0.02 M iodine solution at the oxidation step to avoid degradation of the cyanine dye.

Deprotection: At room temperature with 30% aqueous ammonium solution. It is recommended to use nucleic bases with labile protective groups for deprotection for not more than 2 h at less than 55°C. AMA (30% aqueous ammonium solution/40% aqueous methylamine 1:1 (v/v)) can be used for 10 min at 65°C in the presence of acetyldeoxycytidine. If deoxyguanidine with a dimethylformamidine protective group is used during synthesis, deprotect with 30% aqueous ammonium solution for 2 h at 65°C. If deoxyguanidine with an isobutyryl protective group is used during synthesis, deprotect for 24-36 h at room temperature.

Cy3 是一种荧光团,广泛用于分子生物学实验,例如寡核苷酸标记和寡核苷酸检测。根据其光谱特性,Cyanine3 是 Cy™3 的类似物,在黄色光谱范围内的荧光最大值为 570 nm。

花青 3 亚磷酰胺 5′ 用于寡核苷酸合成,以生产 5′-花青 3 标记的寡核苷酸。该试剂与各种寡核苷酸合成仪兼容。

这种亚磷酰胺可用于合成荧光标记的引物和杂交探针,例如 TaqMan 和 Molecular Beacon。可以在 TAMRA 通道中的多重实时 PCR 中检测到此类标记的探针。

冷凝:3 分钟。在氧化步骤中使用 0.02 碘溶液以避免花青染料降解。

脱保护:在室温下用 30% 的铵水溶液。建议使用带有不稳定保护基团的核酸碱基在低于 55°C 的条件下脱保护不超过 2 小时。 AMA(30% 铵水溶液/40% 甲胺水溶液 1:1 (v/v))可在乙酰脱氧胞苷存在下在 65°C 下使用 10 分钟。如果在合成过程中使用带有二甲基甲脒保护基团的脱氧胍,则在 65°C 下用 30% 铵水溶液脱保护 2 小时。如果在合成过程中使用带有异丁酰基保护基团的脱氧胍,则在室温下脱保护 24-36 小时。

Cyanine3 phosphoramidite 5’相关产品

Cyanine5.5 phosphoramidite, 5′-terminal

Amidite for the synthesis of oligonucleotides and qPCR probes bearing 5′-terminal dye Cyanine5.5. Useful dye for multiplex qPCR.

Amidite 用于合成带有 5′-末端染料 Cyanine5.5 的寡核苷酸和 qPCR 探针。 用于多重 qPCR 的有用染料。

FAM Phosphoramidite, 6-Isomer

Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.

用于合成修饰寡核苷酸的单一异构体 6-FAM 亚磷酰胺。

Cyanine5 phosphoramidite

Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.

Cyanine5 的亚磷酰胺衍生物,用于寡核苷酸合成。

General properties

Appearance: red powder
Molecular weight: 841.81
Molecular formula: C45H65N5BF4O3P
Solubility: good in acetonitrile, dichloromethane
Quality control: NMR 1H, 31P, HPLC-MS (80%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible

建议在 +4°C 或超温和保护基团下放置 48 小时; 24 小时在 rt 可能

Cy™ is a registered trademark of Cytiva in some countries.

Cyanine3 DBCO

Cyanine3 DBCO,偶氮二苯并环辛炔(DBCO 或 ADIBO)是一种稳定但非常活泼的环炔烃,适用于无铜点击化学。

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  • Cyanine3 DBCO

Cyanine3 DBCO

标签:Cy3, Copper free Click chemistry, Cycloalkyne
货号 规格 价格 货期
A10F0 1 mg 110.00$ in stock
B10F0 5 mg 210.00$ in stock
C10F0 10 mg 310.00$ in stock
D10F0 25 mg 410.00$ 5 days
E10F0 50 mg 695.00$ in stock
F10F0 100 mg 1190.00$ in stock

Azodibenzocyclooctyne (DBCO or ADIBO) is a stable but very reactive cycloalkyne for copper free Click chemistry.

This reagent is a conjugate of DBCO with Cyanine3 fluorophore for the non-catalyzed, strain promoted reaction with azides.

偶氮二苯并环辛炔(DBCO 或 ADIBO)是一种稳定但非常活泼的环炔烃,适用于无铜点击化学。

该试剂是 DBCO 与 Cyanine3 荧光团的结合物,用于与叠氮化物的非催化、应变促进反应。

Absorption and emission spectra of Cyanine3 fluorophore

Cyanine3 DBCO

Cyanine3 DBCO相关产品

Cyanine5 tetrazine

Cyanine5 is a universal red emitting dye with a very high extinction coefficient, and good quantum yield. This tetrazine derivative reacts very quickly with trans-cyclooctenes and cyclopropenes with the formation of stable conjugates.

Cyanine5 是一种通用的红色发光染料,具有非常高的消光系数和良好的量子产率。 这种四嗪衍生物与反式环辛烯和环丙烯反应非常迅速,形成稳定的共轭物。

sulfo-Cyanine3 DBCO

Sulfo-Cyanine3 DBCO is a cycloalkyne derivative of water soluble, general purpose fluorophore sulfo-Cyanine3 for Click Chemistry.

Sulfo-Cyanine3 DBCO 是用于 Click Chemistry 的水溶性通用荧光团 sulfo-Cyanine3 的环炔衍生物。

Cyanine7.5 NHS ester

Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5.

NIR荧光染料Cy7.5的胺反应性NHS酯。

General properties

Appearance: dark red powder 暗红色粉末
Molecular weight: 902.99
CAS number: 2692677-79-3
Molecular formula: C51H57N4O2PF6
Solubility: good in DMF, DMSO, DCM, alcohols, practically insoluble in water (< 1 uM, < 1 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

相关文献:

  1. Jiang, Z.; He, H.; Liu, H.; Thayumanavan, S. Azide-Terminated RAFT Polymers for Biological Applications. Current Protocols in Chemical Biology, 2020, 12(4), e85. doi: 10.1002/cpch.85
  2. Islam, M.R.; Nguy, C.; Pandit, S.; Lyon, L.A. Design and Synthesis of Core–Shell Microgels with One‐Step Clickable Crosslinked Cores and Ultralow Crosslinked Shells. Macromolecular Chemistry and Physics, 2020, 221(19), 2000156. doi: 10.1002/macp.202000156
  3. Jiang, Z.; He, H.; Liu, H.; Thayumanavan, S. Cellular Uptake Evaluation of Amphiphilic Polymer Assemblies: Importance of Interplay between Pharmacological and Genetic Approaches. Biomacromolecules, 2019, 20(12), 4407–4418. doi: 10.1021/acs.biomac.9b01073

Sulfo-Cyanine3 amine

Sulfo-Cyanine3 amine,一种带有氨基的水溶性染料,可用于与亲电试剂结合,以及用于酶促氨基转移标记。Sulfo-Cyanine3 是 Cy3® 的磺化类似物,可与各种荧光测量设备兼容。 该染料具有高度的光稳定性。 它也很容易被肉眼检测到。

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  • Sulfo-Cyanine3 amine

Sulfo-Cyanine3 amine

标签:Amines, sulfo-Cyanine3,Cy3
货号 规格 价格 货期
113C0 1 mg 110.00$ in stock
213C0 5 mg 290.00$ in stock
413C0 25 mg 690.00$ in stock
513C0 50 mg 1270.00$ in stock
613C0 100 mg 1990.00$ in stock

Sulfo-Cyanine3 amineA water soluble dye with amino group, useful for the conjugation with electrophiles, and for enzymatic transamination labeling.

Sulfo-Cyanine3 is a sulfonated analog of Cy3®, which is compatible with various fluorescence measuring equipment. The dye is highly photostable. It is also easily detectable by naked eye.

一种带有氨基的水溶性染料,可用于与亲电试剂结合,以及用于酶促氨基转移标记。

Sulfo-Cyanine3 是 Cy3® 的磺化类似物,可与各种荧光测量设备兼容。 该染料具有高度的光稳定性。 它也很容易被肉眼检测到。

Sulfo-Cy3 absorbance and emission spectra

Sulfo-Cyanine3 amine

Sulfo-Cyanine3 amine相关产品

Cyanine5 DBCO

Cyanine5 dye with dibenzocyclooctyne (DBCO) functional group for copper free Click chemistry.

用于无铜点击化学的具有二苯并环辛炔 (DBCO) 官能团的 Cy5 染料。

BDP 558/568 amine

BDP 558/568 is a borondipyrromethene dye with yellow/orange emission. This amine derivative can be conjugated by means of reactions with various electrophiles.

BDP 558/568 是一种具有黄色/橙色发射光的硼二吡咯亚甲基染料。 这种胺衍生物可以通过与各种亲电子试剂的反应来缀合。

Sulfo-Cyanine5 maleimide

Thiol reactive, water soluble cyanine dye for Cy5&reg; channel.

用于 Cy5&reg; 的硫醇活性水溶性花青染料 渠道。

General properties

Appearance: dark red solid 暗红色固体
Molecular weight: 714.94
CAS number: 2183440-43-7
Molecular formula: C36H50N4O7S2
IUPAC name: 3H-​Indolium, 1-​[6-​[(6-​aminohexyl)​amino]​-​6-​oxohexyl]​-​2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​3,​3-​dimethyl-​5-​sulfo-​, inner salt
Solubility: well soluble in water (0.49 M = 350 g/L), alcohols, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

相关文献:

  1. Bos, I.; Timmerman, M.; Sprakel, J. FRET-Based Determination of the Exchange Dynamics of Complex Coacervate Core Micelles. Macromolecules, 2021, 54(1), 398–411. doi: 10.1021/acs.macromol.0c02387
  2. Zhang, Q.; Scigliano, A.; Biver, T.; Pucci, A.; Swager, T.M. Interfacial Bioconjugation on Emulsion Droplet for Biosensors. Bioorganic & Medicinal Chemistry, 2018, 26(19), 5307–5313. doi: 10.1016/j.bmc.2018.04.020
  3. Sobotzki, N.; Schafroth, M.A.; Rudnicka, A.; Koetemann, A.; Marty, F.; Goetze, S.; Yamauchi, Y.; Carreira, E.M.; Wollscheid, B. HATRIC-based identification of receptors for orphan ligands. Nature Communications, 2018, 9, 1519. doi: 10.1038/s41467-018-03936-z

Cyanine3 amine

Cyanine3 amine,Cy3胺是一种含有游离氨基的功能化花青染料。 Cyanine3 是 Cy3® 的类似物

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Cyanine3 amine

标签:Cyanine3, Cy3, Amines
货号 规格 价格 货期
110C0 1 mg 110.00$ 5 days
210C0 5 mg 210.00$ in stock
410C0 25 mg 410.00$ in stock
510C0 50 mg 695.00$ in stock
610C0 100 mg 1190.00$ in stock

Cyanine3 amine is a functionalized cyanine dye containing a free amino group. Cyanine3 is an analog of Cy3®.

Amino group of this reagent can be conjugated with reactive groups such as NHS esters, carboxy groups (after carbodiimide activation), and epoxides.

The amino dye is supplied in salt form, and possesses some aqueous solubility.

Cy3胺是一种含有游离氨基的功能化花青染料。 Cyanine3 是 Cy3® 的类似物。

该试剂的氨基可与 NHS 酯、羧基(碳二亚胺活化后)和环氧化物等反应基团结合。

氨基染料以盐的形式提供,并具有一定的水溶性。

Cyanine3 absorption and emission spectra

Cyanine3 amine

Cyanine3 amine相关产品

Cyanine5.5 azide

Far red / near infrared fluorescent Cyanine5.5 dye derivative for Click Chemistry.

用于 Click Chemistry 的远红/近红外荧光 Cyanine5.5 染料衍生物。

Cyanine7.5 hydrazide

Near infrared fluorophore functionalized with hydrazide moiety. Reactive against carbonyl compounds, useful for the labeling of saccharides, and antibodies after periodate oxidation.

用酰肼部分功能化的近红外荧光团。 对羰基化合物具有反应性,可用于标记糖类和高碘酸盐氧化后的抗体。

General properties

Appearance: red powder  红色粉末
Molecular weight: 627.73
CAS number: 2247688-56-6
Molecular formula: C36H52Cl2N4O
IUPAC name: 3H-​Indolium, 1-​[6-​[(6-​aminohexyl)​amino]​-​6-​oxohexyl]​-​2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​3,​3-​dimethyl-​, chloride, hydrochloride (1:1:1)
Solubility: moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

相关文献:

  1. Wang, Y.; van Steenbergen, M.J.; Beztsinna, N.; Shi, Y.; Lammers, T.; van Nostrum, C.F.; Hennink, W.E. Biotin-decorated all-HPMA polymeric micelles for paclitaxel delivery. Journal of Controlled Release, 2020, 328, 970–984. doi: 10.1016/j.jconrel.2020.09.013
  2. Hifni, B.; Khan, M.; Devereux, S.J.; Byrne, M.H.; Quinn, S.J.; Simpson, J.C. Investigation of the cellular destination of fluorescently labelled carbon nanohorns in cultured cells. ACS Applied Bio Materials, 2020, 3(10), 6790–6801. doi: 10.1021/acsabm.0c00748
  3. Teng, F.; Wu, X.; Libera, M.R. Chemical Orthogonality in Surface-Patterned Poly(ethylene glycol) Microgels. Langmuir, 2020, 36(35), 10622–10627. doi: 10.1021/acs.langmuir.0c02172
  4. Zaw Thin, M.; Allan, H.; Bofinger, R.; Kostelec, T.D.; Guillaume, S.; Connell, J.J.; Patrick, P.S.; Hailes, H.C.; Tabor, A.B.; Lythgoe, M.F.; Stuckey, D.J.; Kalber, T.L. Multi-modal imaging probe for assessing the efficiency of stem cell delivery to orthotopic breast tumours. Nanoscale, 2020, 12(31), 16570–16585. doi: 10.1039/d0nr03237a
Cy® is a trademark of GE Healthcare.

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 carboxylic acid,水溶性磺基-Cy3 染料,游离未活化的单官能羧酸。 该试剂可用作 Cy3® 检测通道的参考荧光团,在其他磺基-Cyanine3 标记产品的实验中用作对照。 羧酸也可以用碳二亚胺活化。

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  • Reactive dyes
  • Carboxylic acids
  • Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 carboxylic acid

标签:Carboxylic acids, sulfo-Cyanine3,Cy3
货号 规格 价格 货期
11390 1 mg 110.00$ in stock
21390 5 mg 210.00$ in stock
41390 25 mg 410.00$ in stock
51390 50 mg 695.00$ in stock
61390 100 mg 1190.00$ in stock

Water soluble sulfo-Cyanine3 dye, free unactivated monofunctional carboxylic acid. This reagent can be used as a reference fluorophore for Cy3® detection channel, as a control in experiments with other sulfo-Cyanine3 labeled products. Carboxylic acid can be also activated with carbodiimides.

Absorbance and emission spectra are identical with Cy3® fluorophore.

水溶性磺基-Cyanine3 染料,游离未活化的单官能羧酸。 该试剂可用作 Cy3® 检测通道的参考荧光团,在其他磺基-Cyanine3 标记产品的实验中用作对照。 羧酸也可以用碳二亚胺活化。

吸光度和发射光谱与 Cy3® 荧光团相同。

Sulfo-Cyanine3 absorbance and emission spectra

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 carboxylic acid相关产品

AF568 azide, 6-isomer

AF 568 derivative designed for different imaging applications

AF 568 衍生产品专为不同的成像应用而设计

THPTA ligand

THPTA is a water soluble ligand for Cu(I) catalyzed Click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.

THPTA 是一种用于 Cu(I) 催化点击化学的水溶性配体。 配体将铜稳定在其 Cu(I) 氧化态。 由于高水溶性,与该配体的反应不需要有机助溶剂。

Cyanine3 amine

Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.

Cy3 是一种带有游离氨基的荧光团(氨基染料)。 它可以与 NHS 酯、羧基(活化后)和环氧化物结合。

General properties

Appearance: dark red crystals 暗红色晶体
Molecular weight: 654.84
CAS number: 1121756-11-3 (inner salt); 1941997-61-0 (sodium salt)
Molecular formula: C30H35N2KO8S2
IUPAC name: 3H-​Indolium, 2-​[3-​[1-​(5-​carboxypentyl)​-​1,​3-​dihydro-​3,​3-​dimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene]​-​1-​propen-​1-​yl]​-​1,​3,​3-​trimethyl-​5-​sulfo-​, inner salt, potassium salt
Solubility: Well soluble in water, DMF, DMSO (0.55 M = 360 g/L). Practically insoluble in non-polar organic solvents.
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

相关文献:

  1. Vallmitjana, A.; Torrado, B.; Dvornikov, A.; Ranjit, S.; Gratton, E. Blind Resolution of Lifetime Components in Individual Pixels of Fluorescence Lifetime Images Using the Phasor Approach. Journal of Physical Chemistry B, 2020, 124(45), 10126–10137. doi: 10.1021/acs.jpcb.0c06946
  2. Cho, U.; Riordan, D.P.; Ciepla, P.; Kocherlakota, K.S.; Chen, J.K.; Harbury, P.B. Ultrasensitive optical imaging with lanthanide lumiphores. Nature Chemical Biology, 2018, 14(1), 15–21. doi: 10.1038/nchembio.2513
  3. Spears, B.R.; Marin, M.A.; Chaker, A.N.; Lampley, M.W.; Harth, E. Precise Microscale Polymeric Networks through Piezoelectronic Inkjet Printing. ACS Biomaterials Science & Engineering, 2016, 2(8), 1265–1272. doi: 10.1021/acsbiomaterials.6b00175
Cy® is a trademark of GE Healthcare.

Cyanine3 carboxylic acid

Cyanine3 carboxylic acid,游离Cyanine3 羧酸(Cy3® 羧酸类似物),非活化染料。 非磺化试剂,在有机溶剂中溶解性好,水溶性有限。 该染料可用作非反应性荧光团,用于对照实验和校准。

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  • Reactive dyes
  • Carboxylic acids
  • Cyanine3 carboxylic acid

Cyanine3 carboxylic acid

标签:Cy3, Cyanine3,Carboxylic acids
货号 规格 价格 货期
11090 1 mg 110.00$ in stock
21090 5 mg 210.00$ in stock
41090 25 mg 410.00$ in stock
51090 50 mg 695.00$ in stock
61090 100 mg 1190.00$ in stock

Free Cyanine3 carboxylic acid (Cy3® carboxylic acid analog), non-activated dye. Non-sulfonated reagent, with good solubility in organic solvents, and limited aquous solubility. The dye can be used as a non-reactive fluorophore, for control experiments, and for calibration.

For coupling with amines, and labeling, consider using Cyanine3 NHS ester, or water-soluble sulfo-Cyanine3 NHS ester.

游离Cyanine3 羧酸(Cy3® 羧酸类似物),非活化染料。 非磺化试剂,在有机溶剂中溶解性好,水溶性有限。 该染料可用作非反应性荧光团,用于对照实验和校准。

对于与胺偶联和标记,请考虑使用 Cyanine3 NHS 酯或水溶性磺基-Cyanine3 NHS 酯。

Cyanine3 absorbance and emission spectra

Cyanine3 carboxylic acid

Cyanine3 carboxylic acid相关产品

FAM NHS ester, 6-isomer

Activated N-hydroxysuccinimide ester of fluorescein (FAM), pure 6-isomer.

荧光素的活化 N-羟基琥珀酰亚胺酯 (FAM),纯 6-异构体。

Cyanine5.5 azide

Far red / near infrared fluorescent Cyanine5.5 dye derivative for Click Chemistry.

用于 Click Chemistry 的远红/近红外荧光 Cy5.5 染料衍生物。

Cyanine3 NHS ester

Amine-reactive Cyanine3 dye NHS ester.

胺反应性 Cy3 染料 NHS 酯。

General properties

Appearance: red powder 红色粉末
Molecular weight: 493.08
CAS number: 1361402-15-4 (inner salt), 1032678-01-5 (chloride), 1251915-29-3 (iodide)
Molecular formula: C30H37ClN2O2
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), poorly soluble in water (1.8 g/L = 4.0 mM)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

相关文献:

  1. Nam, J.; Lim, H.-K.; Kim, N.H.; Park, J.K.; Kang, E.S.; Kim, Y.-T.; Heo, C.; Lee, O.-S.; Kim, S.-G.; Yun, W.S.; Suh, M.; Kim, Y.H. Supramolecular Peptide Hydrogel-Based Soft Neural Interface Augments Brain Signals through Three-Dimensional Electrical Network. ACS Nano, 2020, 14(1), 664–675. doi: 10.1021/acsnano.9b07396
  2. Deshmukh, R.; Biehs, S.-A.; Khwaja, E.; Galfsky, T.; Agarwal, G.S.; Menon, V.M. Long-range resonant energy transfer using optical topological transitions in metamaterials. ACS Photonics, 2018, 5(7), 2737–2741. doi: 10.1021/acsphotonics.8b00484
  3. Mattila, J.T.; Beaino, W.; Maiello, P.; Coleman, M.T.; White, A.G.; Scanga, C.A.; Flynn, J.A.; Anderson, C.J. Positron Emission Tomography Imaging of Macaques with Tuberculosis Identifies Temporal Changes in Granuloma Glucose Metabolism and Integrin α4β1-Expressing Immune Cells. Journal of immunology, 2017, 199(2), 806–815. doi: 10.4049/jimmunol.1700231
  4. Soni, K.S.; Lei, F.; Desale, S.S.; Marky, L.A.; Cohen, S.M.; Bronich, T.K. Tuning polypeptide-based micellar carrier for efficient combination therapy of ErbB2-positive breast cancer. Journal of Controlled Release, 2017, 264, 276–287. doi: 10.1016/j.jconrel.2017.08.038
Cy® is a trademark of GE Healthcare.

Sulfo-Cyanine3 hydrazide

Sulfo-Cyanine3 hydrazide,Sulfo-Cy3 是 Cy3 染料的硫酸化衍生物,由于其结构中有两个带负电荷的磺基,因此可很好地溶于水。根据其光谱特性,这种橙黄色荧光染料是 Cy™3 的类似物。

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  • Reactive dyes
  • Dye hydrazides
  • Sulfo-Cyanine3 hydrazide

Sulfo-Cyanine3 hydrazide

标签:Hydrazides, Microscopy, sulfo-Cyanine3,Cy3
货号 规格 价格 货期
11370 1 mg 110.00$ in stock
21370 5 mg 290.00$ in stock
41370 25 mg 690.00$ in stock
51370 50 mg 1270.00$ in stock
61370 100 mg 1990.00$ in stock

Sulfo-Cyanine3 is a sulfated derivative of Cyanine3 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this yellow-orange fluorescent dye is an analog of Cy™3.

Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules.

The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with Sulfo-Cyanine3 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with Sulfo-Cyanine3 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.

Sulfo-Cy3 是 Cy3 染料的硫酸化衍生物,由于其结构中有两个带负电荷的磺基,因此可很好地溶于水。根据其光谱特性,这种橙黄色荧光染料是 Cy™3 的类似物。

酰肼有效地与醛和酮反应生成腙,因此该化合物可用于与生物分子的羰基衍生物缀合。

反应在水性条件下进行,这在处理抗体和蛋白质时很重要。糖基化蛋白质和抗体中的糖中的 Cys-二醇基团可被氧化成二醛,蛋白质中的半胱氨酸可被酶转化为甲酰基甘油(即与磺基花青 3 酰肼结合的反应基团)。蛋白质和肽中的天冬氨酸和谷氨酸的羧基也可以在活化剂存在的情况下与磺基花青 3 酰肼结合:碳二亚胺 (EDAC) 或甲基吗啉 (DMTMM) 衍生物。

Absorption and emission spectra of sulfo-Cyanine3

Sulfo-Cyanine3 hydrazide

Sulfo-Cyanine3 hydrazide相关产品

Sulfo-Cyanine3 NHS ester

Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.

用于氨基生物分子标记的水溶性磺基-Cy3 SE 活化酯。

Cyanine3 hydrazide

Cyanine3 hydrazide is a carbonyl-reactive dye for Cy3 channel.

Cy3 hydrazide 是 Cy3 通道的羰基反应性染料。

General properties

Appearance: dark red solid 暗红色固体
Molecular weight: 630.78
CAS number: 2144762-62-7
Molecular formula: C30H38N4O7S2
IUPAC name: 3H-​Indolium, 2-​[3-​[1-​(6-​hydrazinyl-​6-​oxohexyl)​-​1,​3-​dihydro-​3,​3-​dimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene]​-​1-​propen-​1-​yl]​-​1,​3,​3-​trimethyl-​5-​sulfo-​, inner salt
Solubility: soluble in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (90%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06
Cy™ is a registered trademark of Cytiva in some countries.

Cyanine3 hydrazide

Cyanine3 hydrazide,Cy3 hydrazide 是一种羰基活性染料,是 Cy3® 酰肼的类似物。

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  • Reactive dyes
  • Dye hydrazides
  • Cyanine3 hydrazide

Cyanine3 hydrazide

标签:Cyanine3,Cy3, Hydrazides, Microscopy
货号 规格 价格 货期
11070 1 mg 110.00$ in stock
21070 5 mg 210.00$ in stock
41070 25 mg 410.00$ in stock
51070 50 mg 695.00$ in stock
61070 100 mg 1190.00$ in stock

Cyanine3 hydrazide is a carbonyl-reactive dye, an analog of Cy3® hydrazide.

This reagent allows to label various carbonyl-containing molecules such as antibodies and other glycoproteins after periodate oxidation, proteins which have undergone oxidative stress or deamination, or reducing saccarides. Cyanine3 is compatible with a number of fluorescent instruments.

Cyanine3 hydrazide 是一种羰基活性染料,是 Cy3® 酰肼的类似物。

该试剂可以标记各种含羰基分子,例如高碘酸氧化后的抗体和其他糖蛋白、经历氧化应激或脱氨基作用的蛋白质或还原糖。 Cyanine3 与许多荧光仪器兼容。

Cyanine3 absorbance and emission spectra

Cyanine3 hydrazide

Cyanine3 hydrazide相关产品

BDP TR hydrazide

BDP TR hydrazide for the labeling of carbonyl compounds with BDP TR, a borondipyrromethene matching ROX channel.

BDP TR 酰肼,用于用 BDP TR 标记羰基化合物,BDP TR 是一种匹配 ROX 通道的硼二吡咯亚甲基。

Cyanine7 NHS ester

Amine reactive NHS ester of near infrared dye Cyanine7.

近红外染料Cy7的胺活性NHS酯。

Cyanine3 azide

Azide derivative of Cyanine3 fluorescent dye for Click Chemistry.

用于 Click Chemistry 的 Cy3 荧光染料的叠氮化物衍生物。

General properties

Appearance: red powder 红色粉末
Molecular weight: 543.57
Molecular formula: C30H40Cl2N4O
Solubility: moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

相关文献:

  1. Zhao, Meng and Börner, Richard and Sigel, Roland K. O. and Freisinger, Eva. Site-Specific Dual-Color Labeling of Long RNAs. Methods in Molecular Biology, 2020, 2106, 253–270. doi: 10.1007/978-1-0716-0231-7_16
  2. Zhang, J.; Chetnani, B.; Cormack, E.D.; Alonso, D.; Liu, W.; Mondragon, A.; Fei, J. Specific structural elements of the T-box riboswitch drive the two-step binding of the tRNA ligand. eLife, 2018, 7, e39518. doi: 10.7554/eLife.39518
  3. Cui, Z.; Wu, Y.; Mureev, S.; Alexandrov, K. Oligonucleotide-mediated tRNA sequestration enables one-pot sense codon reassignment in vitro. Nucleic Acids Research, 2018, 46(12), 6387–6400. doi: 10.1093/nar/gky365
  4. Porciani, D.; Cardwell, L.N.; Tawiah, K.d.; Alam, K.K.; Lange, M.J.; Daniels, M.A.; Burke, D.H. Modular cell-internalizing aptamer nanostructure enables targeted delivery of large functional RNAs in cancer cell lines. Nature Communications, 2018, 9(1), 2283. doi: 10.1038/s41467-018-04691-x
Cy® is a trademark of GE Healthcare.

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine3 maleimide,Sulfo-Cy3 马来酰亚胺是一种水溶性硫醇活性染料,可用于标记亲水性磺基-Cyanine3 荧光团。 这是 Cy3® 马来酰亚胺的类似物。

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  • Reactive dyes
  • Dye maleimides
  • Sulfo-Cyanine3 maleimide

Sulfo-Cyanine3 maleimide

标签:Maleimides, sulfo-Cyanine3,Cy3
货号 规格 价格 货期
11380 1 mg 110.00$ in stock
21380 5 mg 290.00$ in stock
41380 25 mg 690.00$ 5 days
51380 50 mg 1270.00$ in stock
61380 100 mg 1990.00$ in stock

Sulfo-Cyanine3 maleimide is a water soluble, thiol reactive dye for the labeling with hydrophilic sulfo-Cyanine3 fluorophore. This is an analog of Cy3® maleimide.

This product is recommended for the labeling of antibodies and other labile proteins in mild, purely aqueous conditions. The dye is water soluble and does not require use of organic co-solvent.

A non-sulfonated Cyanine3 maleimide is available.

Sulfo-Cyanine3 马来酰亚胺是一种水溶性硫醇活性染料,可用于标记亲水性磺基-Cyanine3 荧光团。 这是 Cy3® 马来酰亚胺的类似物。

该产品推荐用于在温和的纯水性条件下标记抗体和其他不稳定的蛋白质。 该染料是水溶性的,不需要使用有机助溶剂。

可使用非磺化的 Cyanine3 马来酰亚胺。

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine3 maleimide相关产品

Cyanine3 maleimide

Cyanine3 maleimide is a thiol mono reactive dye.

Cy3 马来酰亚胺是一种硫醇单活性染料。

Cyanine7.5 NHS ester

Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5.

NIR荧光染料Cyanine7.5的胺反应性NHS酯。

Sulfo-Cyanine3 NHS ester

Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.

用于氨基生物分子标记的水溶性磺基-Cyanine3 SE 活化酯。

General properties

Appearance: red powder 红色粉末
Molecular weight: 776.96
CAS number: 1656990-68-9
Molecular formula: C36H41KN4O9S2
IUPAC name: 3H-​Indolium, 2-​[3-​[1-​[6-​[[2-​(2,​5-​dihydro-​2,​5-​dioxo-​1H-​pyrrol-​1-​yl)​ethyl]​amino]​-​6-​oxohexyl]​-​1,​3-​dihydro-​3,​3-​dimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene]​-​1-​propen-​1-​yl]​-​1,​3,​3-​trimethyl-​5-​sulfo-​, inner salt, potassium salt
Solubility: soluble in water (0.39 M = 30 g/L), DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95+%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

相关文献:

  1. Ast, J.; Novak, A.N.; Podewin, T.; Fine, N.H.F.; Jones, B.; Tomas, A.; Birke, R.; Roßmann, K.; Mathes, B:; Eichhorst, J.; Lehmann, M.; Linnemann, A.K.; Hodson, D.J.; Broichhagen, J. An expanded LUXendin color palette for GLP1R detection and visualization in vitro and in vivo. ChemRxiv, preprint. doi: 10.33774/chemrxiv-2021-7rngq
  2. Mohale, M.; Gundampati, R.K.; Thallapuranam, S.; Heyes, C.D. Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent dyes in the flexible N-Terminal region and a rigid β-turn region. Analytical Biochemistry, 2022, 640, 114524. doi: 10.1016/j.ab.2021.114524
  3. Bauer, B.W.; Davidson, I.F.; Canena, D.; Wutz, G.; Tang, W.; Litos, G.; Horn, S.; Hinterdorfer, P.; Peters, J.-M. Cohesin mediates DNA loop extrusion by a “swing and clamp” mechanism. Cell, 2021, 184(21), 5448–5464.e22. doi: 10.1016/j.cell.2021.09.016
  4. Deckard, C.E.; Sczepanski, J.T. Reversible chromatin condensation by the DNA repair and demethylation factor thymine DNA glycosylase. Nucleic Acids Research, 2021, 49(5), 2450–2459. doi: 10.1093/nar/gkab040
Cy® is a trademark of GE Healthcare.

Cyanine3 maleimide

Cyanine3 maleimide,硫醇单反应性 Cyanine3 染料。 该试剂可用于将 Cy3 荧光团(Cy3® 的类似物)连接到含有半胱氨酸残基的蛋白质和肽以及其他硫醇化分子(例如含硫醇的寡核苷酸)上。

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  • Cyanine3 maleimide

Cyanine3 maleimide

标签:Cyanine3, Cy3,Maleimides, Microscopy
货号 规格 价格 货期
11080 1 mg 110.00$ in stock
21080 5 mg 210.00$ in stock
41080 25 mg 410.00$ in stock
51080 50 mg 695.00$ in stock
61080 100 mg 1190.00$ in stock

Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).

Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.

Labeling with Cyanine3 maleimide is selective, and efficient.

We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.

硫醇单反应性 Cyanine3 染料。 该试剂可用于将 Cyanine3 荧光团(Cy3® 的类似物)连接到含有半胱氨酸残基的蛋白质和肽以及其他硫醇化分子(例如含硫醇的寡核苷酸)上。

标记前应使用 TCEP(三羧乙基膦)或其他合适的还原剂还原胱氨酸。

用 Cyanine3 马来酰亚胺标记具有选择性且有效。

我们建议使用水溶性 Sulfo-Cyanine3 马来酰亚胺标记抗体和其他敏感蛋白。

Cy3 absorbance and emission spectra

Cyanine3 maleimide

Cyanine3 maleimide相关产品

Cyanine3 NHS ester

Amine-reactive Cyanine3 dye NHS ester.

胺反应性 Cy3 染料 NHS 酯。

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 free unactivated monofunctional carboxylic acid, water-soluble dye.

Sulfo-Cyanine3 不含未活化的单官能羧酸,水溶性染料。

Cyanine3 carboxylic acid

Free Cyanine3 carboxylic acid.

游离Cy3羧酸。

General properties

Appearance: red powder  红色粉末
Molecular weight: 666.56
Molecular formula: C36H43N4O3BF4
Solubility: well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

相关文献:

  1. Yang, M.; Zhang, L.; Sun, R.; Zhong, W.; Yang, Y.; Chen, C. Streptococcus pyogenes Cas9 displays biased one-dimensional diffusion on dsDNA to search for a target. bioRxiv, preprint. doi: 10.1101/754564
  2. Budiardjo, S.J.; Deay, J.J.; Calkins, A.L.; Wimalasena, V.K.; Montezano, D.; Biteen, J.S.; Slusky, J.S.G. Colicin E1 Fragments Potentiate Antibiotics by Plugging TolC. bioRxiv, preprint. doi: 10.1101/692251
  3. Mohale, M.; Gundampati, R.K.; Thallapuranam, S.; Heyes, C.D. Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent dyes in the flexible N-Terminal region and a rigid β-turn region. Analytical Biochemistry, 2022, 640, 114524. doi: 10.1016/j.ab.2021.114524
  4. Bagert, J.D.; Mitchener, M.M.; Patriotis, A.L.; Dul, B.E.; Wojcik, F.; Nacev, B.A.; Feng, L.; Allis, C.D.; Muir, T.W. Oncohistone mutations enhance chromatin remodeling and alter cell fates. Nature Chemical Biology, 2021, 17(4), 403–411. doi: 10.1038/s41589-021-00738-1
Cy® is a trademark of GE Healthcare.

Cyanine3 alkyne,Cy3炔烃

Cyanine3 alkyne,Cy3 炔烃是一种可用于 Click Chemistry 反应的荧光团。使用该试剂,明亮且光稳定的 Cyanine3 报告基因(Cy3® 的类似物)可以连接到几乎任何带有叠氮基的分子上。这种炔烃是非水溶性的。 然而,当与有机共溶剂(DMSO 或 DMF)一起使用时,这种染料可以有效地附着在水溶性分子上,例如蛋白质或肽

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  • Reactive dyes
  • Dye alkynes
  • Cyanine3 alkyne

Cyanine3 alkyne

标签:Cyanine3, Cy3,Alkynes, Microscopy
货号 规格 价格 货期
A10B0 1 mg 110.00$ in stock
B10B0 5 mg 210.00$ in stock
C10B0 10 mg 310.00$ in stock
D10B0 25 mg 410.00$ in stock
E10B0 50 mg 695.00$ in stock
F10B0 100 mg 1190.00$ in stock

Cyanine3 alkyne is a fluorophore ready for the use in Click Chemistry reaction.

With this reagent, bright and photostable Cyanine3 reporter, an analog of Cy3®, can be attached to nearly any molecule bearing azide group.

This alkyne is non water-soluble. However, when used with organic co-solvent (DMSO or DMF), this dye can be efficiently attached to water-soluble molecules such as proteins, or peptides.

Cy3 炔烃是一种可用于 Click Chemistry 反应的荧光团。

使用该试剂,明亮且光稳定的 Cyanine3 报告基因(Cy3® 的类似物)可以连接到几乎任何带有叠氮基的分子上。

这种炔烃是非水溶性的。 然而,当与有机共溶剂(DMSO 或 DMF)一起使用时,这种染料可以有效地附着在水溶性分子上,例如蛋白质或肽。

Cyanine3 absorbance and emission spectra

Cyanine3 alkyne

Cyanine3 alkyne相关产品

BDP 558/568 alkyne

BDP 558/568 is a borondipyrromethene dye for Cyanine3 channel. This is a terminal alkyne derivative.

BDP 558/568 是一种用于 Cy3 通道的硼二吡咯亚甲基染料。 这是一种末端炔烃衍生物。

Sulfo-Cyanine7 amine

Water-soluble NIR dye amine, sulfonated derivative of Cyanine7 dye, for the modification via enzymatical transamination.

水溶性 NIR 染料胺,Cy7 染料的磺化衍生物,用于通过酶促氨基转移进行修饰。

Cyanine3 carboxylic acid

Free Cyanine3 carboxylic acid.

游离Cyanine3羧酸。

General properties

Appearance: red powder 红色粉末
Molecular weight: 530.14
Molecular formula: C33H40ClN3O
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), , practically insoluble in water (50 mg/L = 80 uM)
Quality control: NMR 1H (95%), HPLC-MS
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

相关文献:

  1. Guarin, M.; Faelens, R.; Giusti, A.; De Croze, N.; Léonard, M.; Cabooter, D.; Annaert, P.; de Witte, P.; Ny, A. Spatiotemporal Imaging and Pharmacokinetic of Fluorescent Compounds in Zebrafish Eleuthero-Embryos After Different Routes of Administration. Research Square, preprint. doi: 10.21203/rs.3.rs-237483/v1
  2. Navarro, L.A.; French, D.L.; Zauscher, S. Synthesis of Modular Brush Polymer-Protein Hybrids using Diazotransfer and Copper Click Chemistry. Bioconjugate Chemistry, 2018, 29(8), 2594–2605. doi: 10.1021/acs.bioconjchem.8b00309
  3. Sacoman, J.L.; Dagda, R.Y.; Burnham-Marusich, A.R.; Dagda, R.K.; Berninsone, P.M. Mitochondrial O-GlcNAc transferase (mOGT) regulates mitochondrial structure, function and survival in HeLa cells. Journal of Biological Chemistry, 2017, 292(11), 4499–4518. doi: 10.1074/jbc.M116.726752
  4. Ponomarenko, A.I.; Brylev, V.A.; Sapozhnikova, K.A.; Ustinov, A.V.; Prokhorenko, I.A.; Zatsepin, T.S.; Korshun, V.A. Tetrahedral DNA conjugates from pentaerythritol-based polyazides. Tetrahedron, 2016, 72(19), 2386–2391. doi: 10.1016/j.tet.2016.03.051
Cy2® is a trademark of GE Healthcare.

Sulfo-Cyanine3 azide

Sulfo-Cyanine3 azide,Sulfo-Cyanine3 azide 是一种用于 Click Chemistry 的水溶性染料叠氮化物。 染料的吸光度和发射与 Cy3® 荧光团相同。

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  • Reactive dyes
  • Dye azides
  • Sulfo-Cyanine3 azide

Sulfo-Cyanine3 azide

标签:Azides, Microscopy, sulfo-Cyanine3,Cy3
货号 规格 价格 货期
A1330 1 mg 110.00$ in stock
B1330 5 mg 290.00$ in stock
C1330 10 mg 470.00$ in stock
D1330 25 mg 690.00$ in stock
E1330 50 mg 1270.00$ in stock
F1330 100 mg 1990.00$ in stock

Sulfo-Cyanine3 azide is a water-soluble dye azide for Click Chemistry. Absorbance and emission of the dye are identical to Cy3® fluorophore.

Brightness and photostability of fluorophore, together with high water solubility, makes this reagent an ideal choice for Click chemistry labeling of various molecules in aqueous phase.

Click reaction can be carried out in pure water without need to use organic co-solvent. The reagent is designed for the labeling of sensitive molecules such as proteins, and even intact biological objects.

Sulfo-Cyanine3 azide 是一种用于 Click Chemistry 的水溶性染料叠氮化物。 染料的吸光度和发射与 Cy3® 荧光团相同。

荧光团的亮度和光稳定性以及高水溶性使该试剂成为水相中各种分子的点击化学标记的理想选择。

点击反应可在纯水中进行,无需使用有机助溶剂。 该试剂设计用于标记蛋白质等敏感分子,甚至是完整的生物对象。

Sulfo-Cyanine3 absorbance and emission spectra

Sulfo-Cyanine3 azide

Sulfo-Cyanine3 azide相关产品

Cyanine3 amine

Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.

Cy3 是一种带有游离氨基的荧光团(氨基染料)。 它可以与 NHS 酯、羧基(活化后)和环氧化物结合。

Pico488 dsDNA quantification reagent, 200x solution in DMSO

Pico488 is a dye for precise fluorescence based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.

Pico488 是一种染料,用于在存在 RNA、ssDNA 和其他杂质的情况下对 dsDNA 进行基于荧光的精确定量。 该染料在荧光素通道中显示出明亮的荧光,在低 pg/mL 至高 ng/mL 范围内对 DNA 浓度提供线性响应。

Cyanine3 maleimide

Cyanine3 maleimide is a thiol mono reactive dye.

Cy3 马来酰亚胺是一种硫醇单活性染料。

General properties

Appearance: dark red crystals 暗红色晶体
Molecular weight: 736.94
CAS number: 2055138-89-9 (sodium salt), 1658416-54-6
Molecular formula: C33H41N6KO7S2
Solubility: soluble in water (0.62 M = 46 g/L), DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

相关文献:

  1. Wells, C.I.; Al-Ali, H.; Andrews, D.M.; Asquith, C.R.M.; Axtman, A.D.; Chung, M.; Dikic, I.; Ebner, D.; Elkins, J.M.; Ettmayer, P.; Fischer, C.; Frederiksen, M.; Gray, N.S.; Hatch, S.; Knapp, S.; Lee, S.; Lücking, U.; Michaelides, M.; Mills, C.E.; Müller, S.; Owen, D.; Picado, A.; Ramadan, K.; Saikatendu, K.S.; Schröder, M.; Stolz, A.; Tellechea, M.; Treiber, D.K.; Turunen, B.J.; Vilar, S.; Wang, J.; Zuercher, W.J.; Willson, T.M.; Drewry, D.H. The Kinase Chemogenomic Set (KCGS): An open science resource for kinase vulnerability identification. bioRxiv, preprint. doi: 10.1101/2019.12.22.886523
  2. Li, D.; Taylor, D.H.; van Wolfswinkel, J.C. PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation. Cell Reports, 2021, 37(1), 109776. doi: 10.1016/j.celrep.2021.109776
  3. Wang, J.; Li, T.; Wang, J.-L.; Xu, Z.; Meng, W.; Wu, Q.-F. Talpid3-Mediated Centrosome Integrity Restrains Neural Progenitor Delamination to Sustain Neurogenesis by Stabilizing Adherens Junctions. Cell Reports, 2020, 33(11), 108495. doi: 10.1016/j.celrep.2020.108495
  4. Zahnreich, S.; Weber, B.; Rösch, G.; Schindler, D.; Schmidberger, H. Compromised repair of radiation-induced DNA double-strand breaks in Fanconi anemia fibroblasts in G2. DNA Repair, 2020, 96, 102992. doi: 10.1016/j.dnarep.2020.102992
Cy® is a trademark of GE Healthcare.