货号：CK17
细胞活性/毒性双重检测试剂盒
Viability/Cytotoxicity Multiplex Assay Kit
储存条件：0-5度保存
运输条件：室温
特点：
-
- CCK-8与LDH双指标检测毒性，数据更全面。
- 准备1次细胞，同时检测2项数据。
- 内含500T的CCK-8与LDH检测试剂盒，
- 价格优于单独购买。
选择规格：500 tests
关联产品
NO.1. ROS Assay Kit -Highly Sensitive DCFH-DA- ROS检测
NO.2. Annexin V, FITC Apoptosis Detection Kit 细胞凋亡检测
NO.3. Calcein-AM/PI Double Staining Kit 活死细胞双染检测
NO.4. Cell Cycle Assay Kit 细胞周期检测
NO.5. Caspase-3 Assay Kit-Colorimetric- 细胞凋亡检测
试剂盒内含
500 tests Dye Mixture 5mlX1
[500 tests]
.WST-8,1-Methoxy PMS混合液
Cytotoxicity LDH Assay Kit-WST
[500 tests]
.Dye Mixture X1
.Assay Buffer 15.5mlX1
.Lysis Buffer 155mlX1
.Stop Solutin 27.5mlX1
产品概述
细胞毒性检测通常通过检测活细胞数量或者死细胞数量来确定。而为了更加全面的评价细胞毒性情况，多种评价方法相互验证的需求与日俱增。Viability/CytotoxicityMultiplex Assay Kit可以综合评价细胞毒性。试剂盒内含Cell Counting Kit-8 (CCK-8) 和Cytotoxicity LDH Assay Kit-WST® (LDH Assay Kit)，分别用于测定活细胞和死细胞数量。
CCK-8通过检测活细胞中脱氢酶活性来确定活细胞数，而LDH Assay Kit通过检测受损细胞膜释放LDH的量来确定死细胞数。LDH Assay Kit可以直接添加到含有细胞的培养基中检测 (一步法)。也可以分离细胞后，加到细胞培养基上清液中检测 (低损伤法，分离的细胞可以做其它实验)。两种细胞毒性评价指标，CCK-8和LDH Assay Kit(低损伤法) 可以使用同一批细胞同时进行实验。
产品特点
不同细胞毒性指标，双重印证。增加结果可信性。
单独检测CCK-8，细胞毒性判断不准确
1、单纯检测活细胞数量（CCK-8、MTT、MTS、XTT等）
2种指标一起检测，增加数据准确性
1、单纯检测活细胞数量（CCK-8、MTT、MTS、XTT等）
2、活细胞检测+死细胞检测（细胞膜损伤）
检测原理
LDH Assay Kit：细胞膜损伤，LDH外漏，通过检测LDH活性判断细胞毒性。
CCK-8：可以使用NADH作为指标来确认活细胞中的代谢活性。
同时检测的操作方法
本试剂盒包括用于测量活细胞的CCK-8和用于测量死细胞的LDH分析试剂盒，两者均可通过平板分析法在相同的吸收波长下进行评估。 此外，同一细胞样本可用于同时评估活细胞和死细胞，从而节省了时间和精力。
性价比高
本产品作为CCK-8（500 tests）与Cytotoxicity LDH Assay Kit（500 tests）组合套装，低于单独购买的总价。适合新接触细胞毒性检测的用户，用于前期摸索实验条件。单独产品链接见下方。大包装购买，单价会降低很多。
Cell Counting Kit-8
Cytotoxicity LDH Assay Kit-WST
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