标签： CD14 Human HEK

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14.

CD14 receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol and functions as a high affinity receptor for complexes of LPS and LPS binding protein . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.

CD14 受体）在单核细胞和巨噬细胞上强烈表达，在中性粒细胞表面表达较弱。 CD14 通过与糖基磷脂酰肌醇连接而锚定在细胞上，并作为 LPS 和 LPS 结合蛋白复合物的高亲和力受体发挥作用。 可溶性 CD14 也与 LPS 结合，在生理浓度下作为 LPS 激动剂起作用，并且在较高浓度下，在细胞活化中具有 LPS 拮抗作用。 CD14 已显示与凋亡细胞结合。

CD14 Human Recombinant produced by mammalian expression system in human cells is a single polypeptide chain containing 341 amino acids . CD14 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

CD14 Human Recombinant 由哺乳动物表达系统在人细胞中产生，是一条含有 341 个氨基酸的多肽链。 CD14 在 C 端与 8 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

HEK293 cells.

Sterile Filtered White lyophilized powder.

CD14 was lyophilized from a 0.2 µM filtered solution of 20mM PB and 150mM NaCl, PH 7.4.

It is recommended to reconstitute the lyophilized CD14 in 1xPBS to a concentration no less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD14 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Greater than 95% as determined by -PAGE.

TTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVD
ADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKIT
GTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAF
SCEQVRAFPALTSLDLSDNPGLGERGLMAALCPHKFPAIQNLALRNTGMETPTGVCA
ALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKL
RVLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSMNSGVVPACVDHHHHHH

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