长期暴露在太阳紫外线辐射下可能会对皮肤(包括皮肤癌)、眼睛和免疫系统造成有害的急性和慢性影响。这些有害影响似乎与紫外线引起的DNA损伤密切相关。事实上，紫外线诱导的DNA损伤在细胞周期阻滞、DNA修复激活、细胞杀伤、突变和肿瘤转化等方面发挥着重要作用。太阳紫外线辐射诱导DNA损伤的主要类型是环丁烷嘧啶二聚体(CPDs)、(6-4)光产物(6-4PPs)和6-4PPs的杜瓦价异构体(杜瓦光产物;dewarpp)形成于同一DNA链上相邻的嘧啶核苷酸之间。大约70-80%的紫外线引起的DNA损伤是cpd，其余是6-4PPs和6-4PPs的杜瓦异构体。dewarpp是由6-4PPs在325 nm左右的紫外辐射下光异构化而成。在正常的人类细胞中，这些类型的DNA损伤是通过核苷酸切除修复(NER)修复的。
为了更好地研究围绕紫外线诱导的DNA损伤和修复的分子事件，Mori等人此前开发并鉴定了针对cpd的特异性单克隆抗体(mAb)和针对6-4PPs的特异性单抗(1)，而Matsunaga等人开发并鉴定了针对DewarPPs的特异性单抗(2)。其中三种抗体(cpd:克隆TDM-2;6-4PPs:克隆64M-2;DewarPPs:克隆DEM-1)继续在文献中被频繁引用，通常用于ELISA。
本高灵敏度(6-4)光产物(6-4PPs)酶联免疫吸附试验试剂盒是唯一一种使用抗6-4PPs克隆64M-2的市售酶联免疫吸附试验试剂盒，已优化用于从培养细胞或皮肤表皮纯化的DNA中检测6-4PPs的高灵敏度。该ELISA试剂盒可检测所有DNA序列上下文(即TT, TC, CT和CC)中的6-4PPs。因此，该ELISA试剂盒的可用性和便捷性将有助于进一步了解细胞对紫外线辐射和DNA损伤反应的分子机制，并应用于许多研究领域，包括癌症研究、光生物学、皮肤病学、眼科、免疫学和化妆品科学。
Cosmo Bio抗体,Cosmo Bio,Anti 6-4 Photoproducts (6-4PPs) mAb (Clone 64M-2),CAC-NM-DND-002
Application: ICC, ELISA, WB, IP, IHC, FC
Clonality: Monoclonal
Host: Mouse
Purification: Ammonium Sulfate
Reactivity: All
Prolonged exposure to solar UV radiation may result in harmful acute and chronic effects to the skin (including skin cancers), eye, and immune system. These harmful effects appear to be closely related to UV-induced DNA damage. Indeed, UV-induced DNA damage plays significant roles in cell-cycle arrest, activation of DNA repair, cell killing, mutation, and neoplastic transformation. The major types of DNA damage induced by solar UV radiation are cyclobutane pyrimidine dimers (CPDs), (6–4) photoproducts (6-4PPs), and Dewar valence isomers of 6-4PPs (Dewar photoproducts; DewarPPs) formed between adjacent pyrimidine nucleotides on the same DNA strand. Approximately 70-80% of UV-induced DNA damage is CPDs and the remaining is 6-4PPs and Dewar isomers of 6-4PPs. DewarPPs are produced by the photoisomerization of 6-4PPs by UV radiation around 325 nm. In normal human cells these types of DNA lesions are repaired by nucleotide excision repair (NER).
To better study molecular events surrounding UV-induced DNA damage and repair, Mori et al. previously developed and characterized monoclonal antibody (mAb) specific for CPDs and mAb specific for 6-4PPs (1) while Matsunaga et al. developed and characterized mAb specific for DewarPPs (2). Three of these antibodies (CPDs: clone TDM-2; 6-4PPs: clone 64M-2; DewarPPs: clone DEM-1) continue to be cited frequently in the literature, often for use in ELISA.
This High Sensitivity (6-4)photoproducts (6-4PPs) ELISA Kit is the only commercially available ELISA utilizing anti- 6-4PPs clone 64M-2 and has been optimized for high sensitivity detection of 6-4PPs in DNA purified from cultured cells or from skin epidermis. This ELISA detects 6-4PPs from dipyrimidines in all DNA sequence contexts (i.e., TT, TC, CT and CC). Thus, the availability and convenience of this ELISA Kit will contribute to further understanding molecular mechanisms involved in cellular responses to UV radiation and DNA damage with applications across many research fields including cancer research, photobiology, dermatology, ophthalmology, immunology, and cosmetics science.