植物细胞程序性死亡(PCD)是植物发育和防御机制的重要组成部分。在动物中，不同类型的细胞死亡(凋亡、自噬和坏死)已在形态学上进行了区分，并在这些形态学术语中进行了讨论。PCD主要用于描述细胞凋亡和自噬的过程(尽管有些人将PCD和细胞凋亡互换使用)，而坏死通常被描述为一种混乱和不受控制的死亡模式。在植物中，PCD一词被广泛用于描述观察到的大多数死亡实例。目前，不同的研究小组正在研究大量的植物细胞培养模型和发育系统，从大量文献中所描述的可以清楚地看出，与动物一样，植物中似乎不只有一种类型的PCD。能够区分不同类型的细胞死亡是至关重要的，原因有几个。例如，很明显，在细胞培养系统中，不同小组研究“PCD”的时间窗口差异很大，这可能对数据的解释产生深远的影响，并使比较不同研究人员的数据的尝试复杂化。此外，不同类型的PCD可能有不同的监管机构和死亡方式。因此，在植物细胞培养中，已鉴定出一种凋亡样PCD (AL-PCD)，其速度相当快，并在细胞色素和其他凋亡蛋白释放4-6小时后产生明显的尸体形态。这种类型的形态，不同于自噬和坏死，也在植物发育的例子中观察到。
ATG8是一个自噬相关基因，LC3的同源蛋白。ATG8通过ATG7和ATG3 e1类酶系统与磷脂酰乙醇胺结合在其COOH末端甘氨酸上。高等植物有多个ATG8同源体，包括拟南芥中的ATG8a-g和ATG8h-i两个亚科。抗大豆ATG8i抗体可与植物ATG8i-和ATG8i相关蛋白交叉反应，但与植物ATG8a-g无交叉反应。
Cosmo Bio抗体,CAC-KYU-TY-P01
Application: IP, WB
Clonality: Polyclonal
Host: Rabbit
Purification: Serum
Reactivity: Soybean, Arabidopsis, Plant
Programmed cell death (PCD) in plants is a crucial component of development and defense mechanisms. In animals, different types of cell death (apoptosis, autophagy, and necrosis) have been distinguished morphologically and discussed in these morphological terms. PCD is largely used to describe the processes of apoptosis and autophagy (although some use PCD and apoptosis interchangeably) while necrosis is generally described as a chaotic and uncontrolled mode of death. In plants, the term PCD is widely used to describe most instances of death observed. At present, there is a vast array of plant cell culture models and developmental systems being studied by different research groups and it is clear from what is described in this mass of literature that, as with animals, there does not appear to be just one type of PCD in plants. It is fundamentally important to be able to distinguish between different types of cell death for several reasons. For example, it is clear that, in cell culture systems, the window of time in which ‘PCD’ is studied by different groups varies hugely and this can have profound effects on the interpretation of data and complicates attempts to compare different researcher’s data. In addition, different types of PCD will probably have different regulators and modes of death. For this reason, in plant cell cultures an apoptotic-like PCD (AL-PCD) has been identified that is fairly rapid and results in a distinct corpse morphology which is visible 4–6 h after release of cytochrome and other apoptogenic proteins. This type of morphology, distinct from autophagy and from necrosis, has also been observed in examples of plant development.
ATG8 is an autophagy-related gene, a homolog protein of LC3. ATG8 is conjugated with phosphatidyl ethanol amine on its COOH terminal glycine via the ATG7 and ATG3 E1-like enzyme system. Higher plants have multiple ATG8 homologs comprising two subfamilies, ATG8a-g and ATG8h-i in Arabidopsis. Anti-soybean ATG8i antibody was raised against GST-fused GmATG8i and cross reacts with plant ATG8h- and ATG8i-related proteins, but not with plant ATG8a-g.