自噬是一个进化保守的过程，自噬体与溶酶体融合并降解大量细胞质内容物(1)。自噬参与许多生理过程，包括发育、感染、癌症和神经退行性疾病(2)。ATG(自噬相关)基因在酵母中通过遗传筛选被鉴定(3)。Atg7在Atg12和Atg8泛素样缀合系统中作为e1样酶。Atg7将Atg12转移到e2样酶Atg10上，并将Atg12偶联到Atg5上。Atg7也将Atg8转移到另一种e2样酶Atg3上，并将Atg8与磷脂酰乙醇胺结合(4)。许多这些ATG基因在哺乳动物中是保守的。Atg7缺陷的新生儿在出生后不久就会死于围产期饥饿(5)。神经系统中条件缺失Atg7会导致含泛素聚集物的神经退行性变(6)。
Cosmo Bio抗体,Anti Ubiquitin-Like Modifier-Activating Enzyme ATG7 mAb (Clone ATG7-2),CAC-CTB-AT7-M01
Application: IP, WB
Clonality: Monoclonal
Host: Mouse
Purification: Ig-PG
Reactivity: Human
Autophagy is an evolutionaly conserved process, in which autophagosomes fuse with lysosomes and degrade bulk cytoplasmic contents (1). Autophagy is involved in many physiological processes, including development, infection, cancer, and neurodegenerative diseases (2). ATG (autophagy-related) genes were identified by genetic screening in yeast (3). Atg7 acts as an E1-like enzyme in both Atg12 and Atg8 ubiquitin-like conjugation systems. Atg7 transfers Atg12 to an E2-like enzyme Atg10, and conjugates Atg12 to Atg5. Atg7 also transfers Atg8 to another E2-like enzyme Atg3, and conjugates Atg8 to phosphatidylethanolamine (4). Many of these ATG genes are conserved in mammals. Atg7 deficient neonates die soon after birth from perineonatal starvation (5). Conditional deletion of Atg7 in the nervous system results in neurodegeneration with ubiquitin containing aggregates (6).