标签： BDP 630/650

Lumiprobe 活性荧光染料分类简介

Near infrared (NIR) imaging, BDP 558/568, AF568, Cyanine3.5, Microscopy, sulfo-Cyanine3.5, Protein labeling, AF488, NHS esters, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, Cross linking reagents, AF594, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP 576/589, BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, BDP 564/570, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5, Coumarin 343

Lumiprobe Corporation是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。从2006年起，公司开始生产并销售生命科学研究和诊断学应用的优质化学药品。主要产品有：活性染料（ReactiveDye）和SYBRGreen I染料，定制合成荧光双标记引物。产品主要应用：点击化学（CLickChemistry）、蛋白质组学研究中的双向荧光差异凝胶电泳（2DDIGE）和实时荧光定量PCR（ReaLtimePCR）。Lumiprobe是通过ISO 9001认证的生命科学应用标准和定制试剂制造商。

荧光染料又称为荧光探针, 因具备检测速度快、重复性好、用样量少、无辐射等优点，成为大家广泛使用的荧光标示剂。同时也是细胞生物学等科学研究中不可或缺的重要工具，利用荧光探针可测定RNA和DNA的结构、研究DNA碱基损伤修复、辨别蛋白质分子中氨基的状态和蛋白质分子的活性区, 检测pmol级的蛋白质 ,区分不同构象的核酸以及有关药物的化学反应活性。荧光免疫分析采用时间-分辨技术可用于许多蛋白质、激素、病毒抗原及DNA杂交体的分析。激光诱导荧光光谱在活细胞、活体体液、DNA碱基序列和细菌原体的鉴定、恶性肿瘤的早期诊断和治疗中起到了重要的作用。

由于大多数荧光染料不具备选择性，被固载在生物活性载体的荧光染料，可利用生物活性物质之间的选择性（抗原抗体，生物素链霉亲和素），或者通过染料分子用适当的修饰官能团，通过官能团之间的相互反应，实现对目标物质的靶向性标记，下面我们就来给大家介绍常见的几类活性荧光染料：

活性荧光素及其衍生物广泛用于共价标记生物分子，例如抗体，碳水化合物，核酸，蛋白质，肽和寡核苷酸。各活化官能团对应的反应基团如下：

名称用途
琥珀酰亚胺酯（Succinimidyl esters）/ NHS酯Near infrared (NIR) imaging, BDP 558/568, AF568, Cyanine3.5, Microscopy, sulfo-Cyanine3.5, Protein labeling, AF488, NHS esters, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, Cross linking reagents, AF594, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP 576/589, BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, BDP 564/570, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5, Coumarin 343
荧光染料 NHS 酯是具有活化酯基的胺活性染料。
马来酰亚胺（Maleimides）Near infrared (NIR) imaging, BDP 558/568, Microscopy, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP FL, BDP TMR, Maleimides, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5
染料马来酰亚胺是硫醇反应性荧光染料，用于标记蛋白质中的巯基（硫醇）基团。
叠氮化物(Azides)Near infrared (NIR) imaging, BDP 558/568, AF568, Phenylethynyl-pyrene (PEP), Cyanine3.5, Microscopy, sulfo-Cyanine3.5, AF488, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, HEX (Hexachlorofluorescein), BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Click chemistry, Cyanine7.5, Perylene, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), Azides, AF430, JOE, BDP TR, R6G, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5, Coumarin 343
用于点击化学标记的荧光染料叠氮化物。
炔烃(Alkynes) Near infrared (NIR) imaging, BDP 558/568, AF568, Microscopy, sulfo-Cyanine3.5, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP FL, Alkynes, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, Perylene, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, R6G, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5
用于铜催化点击化学的荧光染料炔烃。含有末端炔基。
酰肼(Hydrazides)Near infrared (NIR) imaging, BDP 558/568, Microscopy, Cyanine7, Cyanine5, BDP 581/591, FAM (Fluorescein), BDP FL, BDP 630/650, Cyanine7.5, TAMRA (TMR, tetramethylrhodamine), Hydrazides, BDP TR, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene
染料酰肼是用于标记醛和酮的羰基反应性荧光染料。
羧酸(Carboxylic acids)Near infrared (NIR) imaging, BDP 558/568, Cyanine3.5, Microscopy, sulfo-Cyanine3.5, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, BDP 581/591, BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, Carboxylic acids, AF430, BDP TR, BDP 564/570, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5, Coumarin 343
荧光染料的羧酸衍生物。
可用于在碳二亚胺预活化后标记胺，也可用于醇的 Steglich 酯化。
氨基(Amines)Near infrared (NIR) imaging, BDP 558/568, Microscopy, Cyanine7, sulfo-Cyanine7, Cyanine5, BDP 581/591, Amines, FAM (Fluorescein), BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, sulfo-Cyanine5.5
具有游离氨基的荧光染料，用于与各种亲电化合物如活化酯和环氧化物偶联。
四嗪（Tetrazines）Near infrared (NIR) imaging, BDP 558/568, Microscopy, Cyanine7, sulfo-Cyanine7, Copper free Click chemistry, Tetrazine, Cyanine5, BDP 581/591, FAM (Fluorescein), BDP 576/589, BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, AF430, BDP TR, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, PEG3 (triethylene glycol)
四嗪是通过反向电子需求[4+2]-环加成反应与反式环辛烯、环丙烯和其他活化烯烃反应的衍生物——无铜点击化学反应的一个例子。
亚磷酰胺(Phosphoramidite)Oligonucleotide synthesis, Cyanine5, TET, HEX (Hexachlorofluorescein), Amines, PEG6 (hexaethylene glycol), FAM (Fluorescein), Alkynes, Phosphoramidite, TAMRA (TMR, tetramethylrhodamine), JOE, R6G, Cyanine5.5, Cyanine3, Pyrene, SIMA
用于固相自动化寡核苷酸合成的亚磷酰胺（亚酰胺）改性剂。
荧光亚磷酰胺，用于点击化学 DNA 修饰的亚酰胺。
环炔(Cycloalkyne)BDP 558/568, Cyanine3.5, Cyanine7, Copper free Click chemistry, Cyanine5, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP FL, BDP TMR, sulfo-Cyanine5, Cycloalkyne, ROX (Rhodamine X, Rhodamine 101), BDP TR, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, sulfo-Cyanine5.5
用于无铜点击化学的具有环炔烃部分的染料。

BDP 630/650 tetrazine

BDP 630/650 tetrazine,BDP 630/650 是一种具有红色发射的硼二吡咯亚甲基染料。 这种荧光团与需要最小背景时经常使用的 Cyanine5 通道相匹配。

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  • BDP 630/650 tetrazine

BDP 630/650 tetrazine

标签：Tetrazine, Copper free Click chemistry, BDP 630/650
货号 规格 价格 货期
154E0 1 mg 110.00$ in stock
254E0 5 mg 290.00$ in stock
454E0 25 mg 690.00$ in stock
554E0 50 mg 1270.00$ in stock
654E0 100 mg 1990.00$ in stock

BDP 630/650 is a borondipyrromethene dye with red emisssion. This fluorophore matches Cyanine5 channel that is often used when minimal background is desired.

Tetrazines react with trans-cyclooctenes in an ultra fast reaction called TCO ligation.

BDP 630/650 是一种具有红色发射的硼二吡咯亚甲基染料。 这种荧光团与需要最小背景时经常使用的 Cyanine5 通道相匹配。

四嗪与反式环辛烯在称为 TCO 连接的超快速反应中发生反应。

Absorption and emission spectra of BDP 630/650

BDP 630/650 tetrazine

BDP 630/650 tetrazine相关产品

FAM tetrazine, 5-isomer

FAM tetrazine (methyltetrazine) for the conjugation with trans-cyclooctenes and cyclopropenes. Pure 5-isomer of fluorescein.

FAM 四嗪（甲基四嗪）用于与反式环辛烯和环丙烯共轭。 荧光素的纯 5-异构体。

ROX DBCO, 5-isomer

ROX (rhodamine X) is a bright rhodamine dye. This is a DBCO (dibenzocyclooctyne) derivative that has high reactivity against azides.

ROX（罗丹明 X）是一种明亮的罗丹明染料。 这是一种对叠氮化物具有高反应性的 DBCO（二苯并环辛炔）衍生物。

Sulfo-Cyanine3 amine

Water soluble cyanine dye with bright fluorescence, and high extinction coefficient. Contains an amino group for the conjugation with electrophiles, and enzymatic transamination labeling.

水溶性花青染料，荧光明亮，消光系数高。 包含用于与亲电试剂结合的氨基和酶促氨基转移标记。

General properties

Appearance: dark colored solid 深色固体
Mass spec M+ increment: 605.2
Molecular weight: 633.48
Molecular formula: C33H26N7BF2O2S
Solubility: good in many organic solvents
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 628
ε, L⋅mol−1⋅cm−1: 97000
Emission maximum, nm: 642
Fluorescence quantum yield: 0.91
CF260: 0.029
CF280: 0.035

BDP 630/650 amine

BDP 630/650 amine,BDP 630/650 是一种远红光发射、基于硼二吡咯亚甲基的荧光团。 该染料经过调整以匹配标准 Cy5 通道，可用作 Cyanine5 和 sulfo-Cyanine5 的替代品。 与花青相比，BDP 630/650 具有更长的荧光寿命，这对于荧光各向异性测量很重要。

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  • BDP 630/650 amine

BDP 630/650 amine

标签：Amines, Fluorescence polarization, BDP 630/650
货号 规格 价格 货期
154C0 1 mg 110.00$ in stock
254C0 5 mg 210.00$ in stock
454C0 25 mg 410.00$ in stock
554C0 50 mg 695.00$ in stock
654C0 100 mg 1190.00$ in stock

BDP 630/650 is a far red emitting, borondipyrromethene based fluorophore. The dye is tuned to match the standard Cy5 channel, and can be used as an alternative to Cyanine5 and sulfo-Cyanine5. Compared to cyanines, BDP 630/650 possesses a longer fluorescence lifetime which is important for fluorescence anisotropy measurements.

BDP 630/650 has a brightness similar to cyanines, and an exceptional photostability.

This amine derivative is useful for the reaction with electrophiles, and for enzymatic transamination labeling.

BDP 630/650 是一种远红光发射、基于硼二吡咯亚甲基的荧光团。 该染料经过调整以匹配标准 Cy5 通道，可用作 Cyanine5 和 sulfo-Cyanine5 的替代品。 与花青相比，BDP 630/650 具有更长的荧光寿命，这对于荧光各向异性测量很重要。

BDP 630/650 具有与花青相似的亮度和出色的光稳定性。

该胺衍生物可用于与亲电试剂的反应以及酶促氨基转移标记。

Absorption and emission spectra of BDP 630/650

BDP 630/650 amine

BDP 630/650 amine相关产品

TAMRA amine, 5-isomer

TAMRA (tetramethylrhodamine) dye, pure 5-isomer, with an amine group for the conjugation with electrophilic reagents (NHS esters, epoxides, etc).

TAMRA（四甲基罗丹明）染料，纯 5-异构体，带有一个胺基，可与亲电子试剂（NHS 酯、环氧化物等）结合。

BDP TR amine

BDP TR borondipyrromethene dye with a free amine group for the conjugation with electrophilic reagents and enzymatic transamination reaction. BDP TR is a bright dye for ROX channel.

带有游离胺基的 BDP TR 硼二吡咯亚甲基染料，用于与亲电试剂偶联和酶促转氨反应。 BDP TR 是 ROX 通道的明亮染料。

BDP 650/665 X NHS ester

Amine reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel, possessing a high molar extinction coefficient, and a good emission quantum yield.

BDP 650/665 的胺反应物，一种用于 Cy5 通道的硼二吡咯亚甲基染料，具有高摩尔消光系数和良好的发射量子产率。

General properties

Appearance: dark violet solid 深紫色固体
Molecular weight: 584.92
Molecular formula: C29H32N4BClF2O2S
Solubility: moderately soluble in water (81 mM = 47.2 mg/mL), well soluble in DMF, DMSO, alcohols
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 628
ε, L⋅mol−1⋅cm−1: 97000
Emission maximum, nm: 642
Fluorescence quantum yield: 0.91
CF260: 0.029
CF280: 0.035

相关文献：

  1. Zhang, Y.; Zhu, X.; Chen, X.; Chen, Q.; Zhou, W.; Guo, Q.; Lu, Y.; Li, C.; Zhang, Y.; Liang, D.; Sun, T.; Wei, X.; Jiang, C. Activated Platelets-Targeting Micelles with Controlled Drug Release for Effective Treatment of Primary and Metastatic Triple Negative Breast Cancer. Advanced Functional Materials, 2019, 29(13), 1806620. doi: 10.1002/adfm.201806620
  2. Zhang, Y.; Guo, Z.; Cao, Z.; Zhou, W.; Zhang, Y.; Chen, Q.; Lu, Y.; Chen, X.; Guo, Q.; Li, C.; Liang, D.; Sun, T.; Jiang, C. Endogenous albumin-mediated delivery of redox-responsive paclitaxel-loaded micelles for targeted cancer therapy. Biomaterials, 2018, 183, 243–257. doi: 10.1016/j.biomaterials.2018.06.002

BDP 630/650 carboxylic acid

BDP 630/650 carboxylic acid,BDP 630/650 是一种基于硼二吡咯亚甲基支架的亮远红色荧光团。 这是一种羧酸衍生物，可以被活化以与胺偶联。 它也可以用作阴性实验中的对照，或者当不需要染料的化学附着时。

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  • BDP 630/650 carboxylic acid

BDP 630/650 carboxylic acid

标签：Carboxylic acids, Fluorescence polarization, BDP 630/650
货号 规格 价格 货期
15490 1 mg 110.00$ in stock
25490 5 mg 210.00$ in stock
45490 25 mg 410.00$ in stock
55490 50 mg 695.00$ in stock
65490 100 mg 1190.00$ in stock

BDP 630/650 is a bright far red fluorophore based on borondipyrromethene scaffold. This is a carboxylic acid derivative that can be activated for the coupling with amines. It can also be used as a control in negative experiments, or when chemical attachment of the dye is not necessary.

BDP 630/650 是一种基于硼二吡咯亚甲基支架的亮远红色荧光团。 这是一种羧酸衍生物，可以被活化以与胺偶联。 它也可以用作阴性实验中的对照，或者当不需要染料的化学附着时。

Absorption and emission spectra of BDP 630/650

BDP 630/650 carboxylic acid

BDP 630/650 carboxylic acid相关产品

Sulfo-Cyanine5 maleimide

Thiol reactive, water soluble cyanine dye for Cy5® channel.

用于 Cy5® 的硫醇活性水溶性花青染料 渠道。

Sulfo-Cyanine5.5 amine

Sulfo-Cyanine5.5 is a far red/NIR fluorophore possessing high hydrophilicity and aqueous solubility. The dye displays good brightness in far red region due to outstanding molar extinction coefficient. This is amine derivative which is reactive towards electrophiles. It can also be used for enzymatic transamination labeling.

Sulfo-Cy5.5 是一种远红/近红外荧光团，具有高亲水性和水溶性。 由于出色的摩尔消光系数，该染料在远红色区域显示出良好的亮度。 这是对亲电试剂有反应性的胺衍生物。 它也可用于酶促转氨标记。

FAM alkyne, 5-isomer

FAM alkyne, 5-isomer – an alkyne derivative of fluorescein dye for Click Chemistry.

FAM 炔烃，5 异构体 – 用于 Click Chemistry 的荧光素染料的炔烃衍生物。

General properties

Appearance: dark powder 黑粉
Molecular weight: 450.27
Molecular formula: C23H17N2BF2O3S
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 628
ε, L⋅mol−1⋅cm−1: 97000
Emission maximum, nm: 642
Fluorescence quantum yield: 0.91
CF260: 0.029
CF280: 0.035

相关文献：

  1. Pfister, J.; Lichius, A.; Summer, D.; Haas, H.; Kanagasundaram, T.; Kopka, K.; Decristoforo, C. Live-cell imaging with Aspergillus fumigatus-specific fluorescent siderophore conjugates. Scientific Reports, 2020, 10, 15519. doi: 10.1038/s41598-020-72452-2
  2. Mitronova, G.Y.; Lukinavičius, G.; Butkevich, A.N.; Kohl, T.; Belov, V.N.; Lehnart, S.E.; Hell, S.W. High-Affinity Functional Fluorescent Ligands for Human β-Adrenoceptors. Scientific Reports, 2017, 7, 12319. doi: 10.1038/s41598-017-12468-3

BDP 630/650 hydrazide

BDP 630/650 hydrazide,BDP 630/650 是一种基于硼二吡咯亚甲基支架的红色发射染料。 这是一种羰基活性染料，可用于标记醛和酮，包括糖。

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  • BDP 630/650 hydrazide

BDP 630/650 hydrazide

标签：Hydrazides, Fluorescence polarization, BDP 630/650
货号 规格 价格 货期
15470 1 mg 110.00$ in stock
25470 5 mg 210.00$ in stock
45470 25 mg 410.00$ in stock
55470 50 mg 695.00$ in stock
65470 100 mg 1190.00$ in stock

BDP 630/650 is a red emitting dye which is based on the borodipyrromethene scaffold. This is a carbonyl reactive dye that is useful for the labeling of aldehydes and ketones, including sugars.

BDP 630/650 fluorophore is particularly useful for fluorescence polarization assays.

BDP 630/650 是一种基于硼二吡咯亚甲基支架的红色发射染料。 这是一种羰基活性染料，可用于标记醛和酮，包括糖。

BDP 630/650 荧光团特别适用于荧光偏振测定。

Absorption and emission spectra of BDP 630/650

BDP 630/650 hydrazide

BDP 630/650 hydrazide相关产品

BDP 581/591 hydrazide

BDP 581/591 is a multi-purpose dye with bright emission. This is a hydrazide dye for the conjugation with aldehydes and ketones.

BDP 581/591 是一种具有明亮发射的多用途染料。 这是一种用于与醛和酮结合的酰肼染料。

BDP 558/568 NHS ester

BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.

BDP 558/568 是光谱黄色区域的荧光团。 与其他 BDP 染料一样，这种荧光团具有高荧光量子产率、亮度和光稳定性。

BDP 558/568 hydrazide

BDP 558/568 hydrazide is a carbonyl reactive dye with emission in orange/yellow part of the spectrum.

BDP 558/568 酰肼是一种羰基活性染料，在光谱的橙色/黄色部分发射。

General properties

Appearance: dark colored solid 深色固体
Mass spec M+ increment: 446.1
Molecular weight: 500.76
Molecular formula: C23H20N4BClF2O2S
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 628
ε, L⋅mol−1⋅cm−1: 97000
Emission maximum, nm: 642
Fluorescence quantum yield: 0.91
CF260: 0.029
CF280: 0.035

BDP 630/650 maleimide

BDP 630/650 maleimide,BDP 630/650 是一种用于 Cy5 通道的远红色发射硼二吡咯亚甲基荧光团。 由于其激发态的寿命相对较长，这种染料对于荧光寿命相关的测量特别有用。

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  • Reactive dyes
  • Dye maleimides
  • BDP 630/650 maleimide

BDP 630/650 maleimide

标签：Maleimides, Fluorescence polarization, BDP 630/650
货号 规格 价格 货期
15480 1 mg 110.00$ in stock
25480 5 mg 210.00$ in stock
45480 25 mg 410.00$ in stock
55480 50 mg 695.00$ in stock
65480 100 mg 1190.00$ in stock

BDP 630/650 is a far red emitting borondipyrromethene based fluorophore for the Cyanine5 channel. Because of its relatively long lifetime of the excited state, this dye is particularly useful for fluorescence lifetime related measurements.

The reactive maleimide moiety allows to attach the dye to thiol groups in biomolecules, especially proteins and peptides.

BDP 630/650 是一种用于 Cy5 通道的远红色发射硼二吡咯亚甲基荧光团。 由于其激发态的寿命相对较长，这种染料对于荧光寿命相关的测量特别有用。

反应性马来酰亚胺部分允许将染料连接到生物分子中的硫醇基团，尤其是蛋白质和肽。

Absorption and emission spectra of BDP 630/650

BDP 630/650 maleimide

BDP 630/650 maleimide相关产品

BDP 650/665 X NHS ester

Amine reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel, possessing a high molar extinction coefficient, and a good emission quantum yield.

BDP 650/665 的胺反应物，一种用于 Cy5 通道的硼二吡咯亚甲基染料，具有高摩尔消光系数和良好的发射量子产率。

Cyanine5.5 azide

Far red / near infrared fluorescent Cyanine5.5 dye derivative for Click Chemistry.

用于 Click Chemistry 的远红/近红外荧光 Cy5.5 染料衍生物。

Azidobutyric acid NHS ester

Activated ester for azido labeling of peptides and proteins.

用于肽和蛋白质的叠氮基标记的活化酯。

General properties

Appearance: dark colored solid 深色固体
Mass spec M+ increment: 572.2
Molecular weight: 572.39
CAS number: 2183473-31-4
Molecular formula: C29H23N4BF2O4S
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 628
ε, L⋅mol−1⋅cm−1: 97000
Emission maximum, nm: 642
Fluorescence quantum yield: 0.91
CF260: 0.029
CF280: 0.035

BDP 630/650 alkyne,BDP 630/650 炔烃

BDP 630/650 alkyne,BDP 630/650 炔烃,BDP 630/650 是一种与 Cy5 通道匹配的红色荧光团。 该染料可用于荧光偏振测定。 它也非常耐光。

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  • Reactive dyes
  • Dye alkynes
  • BDP 630/650 alkyne

BDP 630/650 alkyne

标签：Fluorescence polarization, Microscopy, BDP 630/650,炔烃
货号 规格 价格 货期
A54B0 1 mg 110.00$ in stock
B54B0 5 mg 210.00$ in stock
C54B0 10 mg 310.00$ in stock
D54B0 25 mg 410.00$ in stock
E54B0 50 mg 695.00$ in stock
F54B0 100 mg 1190.00$ in stock

BDP 630/650 is a red emitting fluorophore matching the Cyanine5 channel. The dye is useful for fluorescence polarization assays. It is also remarkably photostable.

The terminal alkyne group can be conjugated with various azides by copper catalyzed Click chemistry.

BDP 630/650 是一种与 Cy5 通道匹配的红色荧光团。 该染料可用于荧光偏振测定。 它也非常耐光。

末端炔基可以通过铜催化的点击化学与各种叠氮化物缀合。

Absorption and emission spectra of BDP 630/650

BDP 630/650 alkyne

BDP 630/650 alkyne,BDP 630/650 炔烃相关产品

BDP TMR azide

Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.

具有叠氮基的硼二吡咯亚甲基染料。 光谱特性类似于 TAMRA 染料。 由于荧光寿命长，BDP TMR 特别适用于荧光偏振测量。

Sulfo-Cyanine3 tetrazine

Water soluble sulfo-Cyanine3 dye tetrazine derivative for TCO ligation labeling with trans-cyclooctenes.

水溶性磺基-Cy3 染料四嗪衍生物，用于用反式环辛烯进行 TCO 连接标记。

EdU (5-ethynyl-2′-deoxyuridine)

EdU is a nucleoside which is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by Click chemistry reaction with fluorescent dye azides to reveal cell proliferation.

EdU 是一种核苷，可通过细胞酶掺入复制的 DNA 中。 之后，细胞中所含的 DNA 可以通过与荧光染料叠氮化物的 Click 化学反应进行显色，以揭示细胞增殖情况。

General properties

Appearance: dark colored solid 深色固体
Mass spec M+ increment: 487.1
Molecular weight: 487.33
CAS number: 2006345-38-4
Molecular formula: C26H20N3BF2O2S
Solubility: good in DMF, DMSO, alcohols
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 628
ε, L⋅mol−1⋅cm−1: 97000
Emission maximum, nm: 642
Fluorescence quantum yield: 0.91
CF260: 0.029
CF280: 0.035

BDP 630/650 azide

BDP 630/650 azide,BDP 630/650 叠氮化物是一种明亮且光稳定的染料叠氮化物，适用于光谱的远红色区域，用于使用 Click 化学进行标记。该染料具有较长的激发态寿命，因此可用于荧光偏振实验。

  • Products
  • Reactive dyes
  • Dye azides
  • BDP 630/650 azide

BDP 630/650 azide

标签：Azides,Fluorescence polarization,Microscopy,BDP 630/650
货号 规格 价格 货期
15430 1 mg 110.00$ in stock
25430 5 mg 210.00$ in stock
35430 10 mg 310.00$ in stock
45430 25 mg 410.00$ in stock
55430 50 mg 695.00$ in stock

BDP 630/650 azide is a bright and photostable dye azide for the far red region of the spectrum, for labeling using Click chemistry.

This dye possesses a relatively long lifetime of the excited state, and can therefore be used for fluorescence polarization experiments.

BDP 630/650 叠氮化物是一种明亮且光稳定的染料叠氮化物，适用于光谱的远红色区域，用于使用 Click 化学进行标记。

该染料具有较长的激发态寿命，因此可用于荧光偏振实验。

BDP 630/650 azide BDP 630/650 azide BDP 630/650 azide BDP 630/650 azide

Absorption and emission spectra of BDP 630/650

BDP 630/650 azide

BDP 630/650 azide相关产品

Cy5.5 maleimide

Cy5.5 maleimide is a thiol reactive dye for protein labeling.
Cy5.5 马来酰亚胺是一种用于蛋白质标记的硫醇活性染料。

TAMRA azide, 5-isomer

TAMRA fluorescent dye azide for Click chemistry.
用于点击化学的 TAMRA 荧光染料叠氮化物。

BDP 630/650 maleimide

BDP 630/650 maleimide is a thiol reactive, borondipyrromethene based dye tuned to match the far red channel of Cyanine5 fluorophore.
BDP 630/650 马来酰亚胺是一种硫醇反应性、基于硼二吡咯亚甲基的染料，经过调整以匹配 Cyanine5 荧光团的远红通道。

General properties

Appearance: dark colored powder
Mass spec M+ increment: 532.2
Molecular weight: 532.37
CAS number: 2183473-22-3
Molecular formula: C26H23N6BF2O2S
IUPAC name: Boron, [N-​(3-​azidopropyl)​-​2-​[4-​[2-​[5-​[[5-​(2-​thienyl)​-​2H-​pyrrol-​2-​ylidene-​κN]​methyl]​-​1H-​pyrrol-​2-​yl-​κN]​ethenyl]​phenoxy]​acetamidato]​difluoro-​, (T-​4)​- Coordination Compound
Solubility: good in DMSO, DMF, acetonitrile, DCM
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 628
ε, L⋅mol−1⋅cm−1: 97000
Emission maximum, nm: 642
Fluorescence quantum yield: 0.91
CF260: 0.029
CF280: 0.035

BDP 630/650 X NHS ester

BDP 630/650 X NHS ester,BDP 630/650 是一种硼二吡咯亚甲基荧光团，具有高摩尔消光系数、出色的量子产率和相对较长的激发态寿命。 因此，这种荧光团可用于荧光偏振测定，以检测分子之间的结合。

  • Products
  • Reactive dyes
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  • BDP 630/650 X NHS ester

BDP 630/650 X NHS ester

标签：NHS esters Fluorescence polarization Microscopy BDP 630/650 Protein labeling
货号 规格 价格 货期
15420 1 mg 110.00$ in stock
25420 5 mg 210.00$ in stock
45420 25 mg 410.00$ in stock
55420 50 mg 695.00$ in stock
65420 100 mg 1190.00$ in stock

BDP 630/650 is a borondipyrromethene fluorophore that has a high molar extinction coefficient, excellent quantum yield, and a relatively long lifetime of the excited state. Due to it, this fluorophore is useful for fluorescence polarization assays that allow to detect binding between molecules.

This is an amine reactive NHS ester. It contains an aminohexanoyl linker between the fluorophore and the reactive group.

BDP 630/650 是一种硼二吡咯亚甲基荧光团，具有高摩尔消光系数、出色的量子产率和相对较长的激发态寿命。 因此，这种荧光团可用于荧光偏振测定，以检测分子之间的结合。

这是一种胺反应性 NHS 酯。 它在荧光团和反应基团之间包含一个氨基己酰基接头。

BDP 630/650 X NHS ester BDP 630/650 X NHS ester BDP 630/650 X NHS ester BDP 630/650 X NHS ester

Absorption and emission spectra of BDP 630/650

BDP 630/650 X NHS ester

BDP 630/650 X NHS ester相关产品

BDP TR X NHS ester

BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used for the conjugation with proteins and peptides.

BDP TR X NHS 酯是 BDP TR 的衍生物，含有一个基于氨基己酸 (C6) 的长接头。 该染料具有接近ROX的吸收和发射。 NHS 酯功能可用于与蛋白质和肽的结合。

FAM tetrazine, 5-isomer

FAM tetrazine (methyltetrazine) for the conjugation with trans-cyclooctenes and cyclopropenes. Pure 5-isomer of fluorescein.

FAM 四嗪（甲基四嗪）用于与反式环辛烯和环丙烯共轭。 荧光素的纯 5-异构体。

dsGreen for Real-Time PCR, 100×

Stock solution of dsGreen for Real-Time PCR, 100×

用于实时 PCR 的 dsGreen 储备溶液，100×

General properties

Appearance: dark colored solid
Mass spec M+ increment: 545.2
Molecular weight: 660.5
CAS number: 2213445-35-1; 380367-48-6
Molecular formula: C33H31N4BF2O6S
IUPAC name: Succinimidyl-6-[2-(p-{(E)-2-[4,4-difluoro-5-(2-thienyl)-3a,4a-diaza-4-bora-s-indacen-3-yl]ethenyl}phenoxy)acetylamino]hexanoate
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 628
ε, L⋅mol−1⋅cm−1: 97000
Emission maximum, nm: 642
Fluorescence quantum yield: 0.91
CF260: 0.029
CF280: 0.035

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  1. Grätz, L.; Tropmann, K.; Bresinsky, M.; Müller, C.; Bernhardt, G.; Pockes, S. NanoBRET binding assay for histamine H2 receptor ligands using live recombinant HEK293T cells. Scientific Reports, 2020, 10, 13288. doi: 10.1038/s41598-020-70332-3
  2. Gruber, C.G.; Pegoli, A.; Müller, C.; Grätz, L.; She, X.; Keller, M. Differently fluorescence-labelled dibenzodiazepinone-type muscarinic acetylcholine receptor ligands with high M2R affinity. RSC Medicinal Chemistry, 2020, 11(7), 823–832. doi: 10.1039/D0MD00137F
  3. Zhong, X.; Shtukenberg, A.G.; Liu, M.; Olson, I.A.; Weck, M.; Ward, M.D.; Kahr, B. Dislocation Generation by Microparticle Inclusions. Crystal Growth & Design, 2019, 19(11), 6649–6655. doi: 10.1021/acs.cgd.9b01041

BDP 630/650 X NHS ester