Lumiprobe 活性荧光染料分类简介

Near infrared (NIR) imaging, BDP 558/568, AF568, Cyanine3.5, Microscopy, sulfo-Cyanine3.5, Protein labeling, AF488, NHS esters, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, Cross linking reagents, AF594, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP 576/589, BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, BDP 564/570, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5, Coumarin 343

Lumiprobe Corporation是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。从2006年起，公司开始生产并销售生命科学研究和诊断学应用的优质化学药品。主要产品有：活性染料（ReactiveDye）和SYBRGreen I染料，定制合成荧光双标记引物。产品主要应用：点击化学（CLickChemistry）、蛋白质组学研究中的双向荧光差异凝胶电泳（2DDIGE）和实时荧光定量PCR（ReaLtimePCR）。Lumiprobe是通过ISO 9001认证的生命科学应用标准和定制试剂制造商。

荧光染料又称为荧光探针, 因具备检测速度快、重复性好、用样量少、无辐射等优点，成为大家广泛使用的荧光标示剂。同时也是细胞生物学等科学研究中不可或缺的重要工具，利用荧光探针可测定RNA和DNA的结构、研究DNA碱基损伤修复、辨别蛋白质分子中氨基的状态和蛋白质分子的活性区, 检测pmol级的蛋白质 ,区分不同构象的核酸以及有关药物的化学反应活性。荧光免疫分析采用时间-分辨技术可用于许多蛋白质、激素、病毒抗原及DNA杂交体的分析。激光诱导荧光光谱在活细胞、活体体液、DNA碱基序列和细菌原体的鉴定、恶性肿瘤的早期诊断和治疗中起到了重要的作用。

由于大多数荧光染料不具备选择性，被固载在生物活性载体的荧光染料，可利用生物活性物质之间的选择性（抗原抗体，生物素链霉亲和素），或者通过染料分子用适当的修饰官能团，通过官能团之间的相互反应，实现对目标物质的靶向性标记，下面我们就来给大家介绍常见的几类活性荧光染料：

活性荧光素及其衍生物广泛用于共价标记生物分子，例如抗体，碳水化合物，核酸，蛋白质，肽和寡核苷酸。各活化官能团对应的反应基团如下：

名称	用途
琥珀酰亚胺酯（Succinimidyl esters）/ NHS酯	Near infrared (NIR) imaging, BDP 558/568, AF568, Cyanine3.5, Microscopy, sulfo-Cyanine3.5, Protein labeling, AF488, NHS esters, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, Cross linking reagents, AF594, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP 576/589, BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, BDP 564/570, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5, Coumarin 343 荧光染料 NHS 酯是具有活化酯基的胺活性染料。
马来酰亚胺（Maleimides）	Near infrared (NIR) imaging, BDP 558/568, Microscopy, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP FL, BDP TMR, Maleimides, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5 染料马来酰亚胺是硫醇反应性荧光染料，用于标记蛋白质中的巯基（硫醇）基团。
叠氮化物(Azides)	Near infrared (NIR) imaging, BDP 558/568, AF568, Phenylethynyl-pyrene (PEP), Cyanine3.5, Microscopy, sulfo-Cyanine3.5, AF488, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, HEX (Hexachlorofluorescein), BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Click chemistry, Cyanine7.5, Perylene, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), Azides, AF430, JOE, BDP TR, R6G, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5, Coumarin 343 用于点击化学标记的荧光染料叠氮化物。
炔烃(Alkynes)	Near infrared (NIR) imaging, BDP 558/568, AF568, Microscopy, sulfo-Cyanine3.5, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP FL, Alkynes, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, Perylene, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, R6G, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5 用于铜催化点击化学的荧光染料炔烃。含有末端炔基。
酰肼(Hydrazides)	Near infrared (NIR) imaging, BDP 558/568, Microscopy, Cyanine7, Cyanine5, BDP 581/591, FAM (Fluorescein), BDP FL, BDP 630/650, Cyanine7.5, TAMRA (TMR, tetramethylrhodamine), Hydrazides, BDP TR, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene 染料酰肼是用于标记醛和酮的羰基反应性荧光染料。
羧酸(Carboxylic acids)	Near infrared (NIR) imaging, BDP 558/568, Cyanine3.5, Microscopy, sulfo-Cyanine3.5, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, BDP 581/591, BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, Carboxylic acids, AF430, BDP TR, BDP 564/570, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5, Coumarin 343 荧光染料的羧酸衍生物。可用于在碳二亚胺预活化后标记胺，也可用于醇的 Steglich 酯化。
氨基(Amines)	Near infrared (NIR) imaging, BDP 558/568, Microscopy, Cyanine7, sulfo-Cyanine7, Cyanine5, BDP 581/591, Amines, FAM (Fluorescein), BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, sulfo-Cyanine5.5 具有游离氨基的荧光染料，用于与各种亲电化合物如活化酯和环氧化物偶联。
四嗪（Tetrazines）	Near infrared (NIR) imaging, BDP 558/568, Microscopy, Cyanine7, sulfo-Cyanine7, Copper free Click chemistry, Tetrazine, Cyanine5, BDP 581/591, FAM (Fluorescein), BDP 576/589, BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, AF430, BDP TR, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, PEG3 (triethylene glycol) 四嗪是通过反向电子需求[4+2]-环加成反应与反式环辛烯、环丙烯和其他活化烯烃反应的衍生物——无铜点击化学反应的一个例子。
亚磷酰胺(Phosphoramidite)	Oligonucleotide synthesis, Cyanine5, TET, HEX (Hexachlorofluorescein), Amines, PEG6 (hexaethylene glycol), FAM (Fluorescein), Alkynes, Phosphoramidite, TAMRA (TMR, tetramethylrhodamine), JOE, R6G, Cyanine5.5, Cyanine3, Pyrene, SIMA 用于固相自动化寡核苷酸合成的亚磷酰胺（亚酰胺）改性剂。荧光亚磷酰胺，用于点击化学 DNA 修饰的亚酰胺。
环炔(Cycloalkyne)	BDP 558/568, Cyanine3.5, Cyanine7, Copper free Click chemistry, Cyanine5, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP FL, BDP TMR, sulfo-Cyanine5, Cycloalkyne, ROX (Rhodamine X, Rhodamine 101), BDP TR, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, sulfo-Cyanine5.5 用于无铜点击化学的具有环炔烃部分的染料。

LumiTracker 溶血红(LumiTracker Lyso Red)

LumiTracker Lyso Red 是一种红色荧光染料，可选择性地积聚在内部 pH 值较低的细胞器中。特别是，它用于通过 ATP 依赖性质子泵对维持低内部 pH 值（即酸性环境）的活细胞中的溶酶体进行染色，从而为消化酶的活性提供最佳条件。

这种染料适用于活体染色；它无需使用额外的洗涤剂即可渗透活细胞的细胞膜。由于染料结构的核心部分属于 BDP 家族，因此具有很强的疏水性，可提供良好的细胞膜渗透性。此外，该染料具有作为弱碱的氨基。它在中性 pH 值下没有完全质子化，染料分子保持相对不带电，有利于更好地通过膜。据推测，在进入溶酶体酸性环境后，染料可能会质子化，从而保留在酸性隔室内。

染料在短时间内被活细胞吸收，染料的质子化促进局部碱化，因此建议在可视化前对活细胞进行不超过 7 分钟的染色。用 LumiTracker Lyso Red 处理后可以固定细胞，但是背景染色水平会变高。一般来说，尽管染料对溶酶体的选择性很高，但建议主要用于成像目的，而不是用于量化溶酶体。

LumiTracker Lyso Red 在 577 nm 处具有最大吸收，在 590 nm 处具有最大发射。我们还提供LumiTracker Lyso Green，用于在绿色通道中进行溶酶体成像（吸收和发射最大值分别为 504 nm 和 511 nm）。

LumiTracker Lyso Red 以 1 mM DMSO 溶液形式提供。

货号	规格
12201	20 x 50 uL

用法

工作浓度：50–75 nM。*

染色：在 37 °C 下在 1× PBS 中染色 3–7 分钟（就在可视化之前）。

*根据细胞系，工作浓度或孵育时间可能会改变

储藏条件

-20°C 黑暗中接收后 12 个月。运输：在室温下长达 3 周。避免长时间暴露在光线下。干燥。避免长时间暴露在光线下和过度冻融循环。如果可能，以一次性使用的等分试样储存。

成套工具

月存储时间： 12

BDP 576/589 tetrazine

BDP 576/589 tetrazine,由于其相对较长的激发态寿命（约 5 纳秒），BDP 576/589 染料可用于基于测量荧光寿命的各种方法。与 BDP 家族的其他染料类似，BDP 576/589 具有很强的疏水性，适用于标记非极性和亲液性生物分子，并随后通过荧光显微镜（包括双光子显微镜）对其进行可视化。

Products
Reactive dyes
Tetrazines
BDP 576/589 tetrazine

BDP 576/589 tetrazine

标签：Tetrazine, BDP 576/589

货号	规格	价格	货期
1G4E0	1 mg	110.00$	in stock
2G4E0	5 mg	290.00$	in stock
3G4E0	10 mg	450.00$	in stock
4G4E0	25 mg	690.00$	in stock
5G4E0	50 mg	1270.00$	in stock
6G4E0	100 mg	1990.00$	in stock

Because of its relatively long excited-state lifetime (about 5 nanoseconds), BDP 576/589 dye can be used in various methods based on measuring fluorescence lifetime. Similarly to other dyes of the BDP family, BDP 576/589 has strong hydrophobic properties and is suitable for labeling non-polar and lypophilic biomolecules and their subsequent visualization by fluorescent microscopy, including two-photon microscopy.

This reagent is a tetrazine derivative that can be conjugated with various strained dienophiles such as trans-cyclooctenes and cyclopropenes. This reaction (TCO ligation) is considered to be one of the best bioconjugation reactions because it is very fast and selective in physiological conditions and does not require additional catalyzers, so it is not toxic in vitro and in vivo.

由于其相对较长的激发态寿命（约 5 纳秒），BDP 576/589 染料可用于基于测量荧光寿命的各种方法。与 BDP 家族的其他染料类似，BDP 576/589 具有很强的疏水性，适用于标记非极性和亲液性生物分子，并随后通过荧光显微镜（包括双光子显微镜）对其进行可视化。

该试剂是一种四嗪衍生物，可与各种应变亲双烯体（例如反式环辛烯和环丙烯）结合。该反应（TCO 连接）被认为是最好的生物偶联反应之一，因为它在生理条件下非常快速且具有选择性，并且不需要额外的催化剂，因此在体外和体内都没有毒性。

BDP 576/589 tetrazine相关产品

BDP 581/591 tetrazine

BDP 581/591 is a borondipyrromethene dye with tetrazine group for the inverse electron demand Diels-Alder (IEDDA) ligation.

BDP 581/591 是一种带有四嗪基团的硼二吡咯亚甲基染料，用于逆电子需求 Diels-Alder (IEDDA) 连接。

BDP 576/589 NHS ester

BDP 576/589 NHS ester (BODIPY™ 576/589 analog) is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.

BDP 576/589 NHS 酯（BODIPY™ 576/589 类似物）是一种明亮的疏水染料，在橙色光谱范围内发射。它可用于荧光各向异性测定。

BDP 576/589 carboxylic acid

Derivative of BDP 576/589 fluorophore (BODIPY™ 576/589 analog) with non-activated free carboxylic acid group.

BDP 576/589 荧光团（BODIPY™ 576/589 类似物）的衍生物，具有未活化的游离羧酸基团。

General properties

Appearance:	dark colored solid 深色固体
Mass spec M+ increment:	501.16
Molecular weight:	529.17
Molecular formula:	C₂₆H₂₃N₈BF₂O
Solubility:	good in polar organic solvents
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. 储存：收到后 24 个月，在 -20°C 避光保存。运输：在室温下长达 3 周。避免长时间暴露在光线下。干燥。
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	580
ε, L⋅mol⁻¹⋅cm⁻¹:	98000
Emission maximum, nm:	592
Fluorescence quantum yield:	0.13

BDP 576/589 NHS ester

BDP 576/589 NHS ester,BDP 576/589（BODIPY™ 576/589 类似物）是一种硼二吡咯亚甲基染料。这种疏水染料具有高量子产率和长激发态寿命（约 5 纳秒）。它可用于测量荧光寿命和荧光偏振以及双光子激光扫描显微镜。

Products
Reactive dyes
Dye NHS esters
BDP 576/589 NHS ester

BDP 576/589 NHS ester

标签：NHS esters Fluorescence polarization Microscopy BDP 576/589

Cat. #	Quantity	Price	Lead time
1G420	1 mg	110.00$	in stock
2G420	5 mg	210.00$	in stock
4G420	25 mg	410.00$	in stock
5G420	50 mg	695.00$	in stock
6G420	100 mg	1190.00$	7 days

BDP 576/589 (a BODIPY™ 576/589 analog) is a boron dipyrromethene dye. This hydrophobic dye has high quantum yield and long excited-state lifetime (about 5 nanoseconds). It can be used for measuring fluorescence lifetime and fluorescence polarization and in two-photon laser scanning microscopy.

This reagent is an activated N-hydroxysuccinimide ester of the dye with reactivity to primary and secondary amino groups.

BDP 576/589（BODIPY™ 576/589 类似物）是一种硼二吡咯亚甲基染料。这种疏水染料具有高量子产率和长激发态寿命（约 5 纳秒）。它可用于测量荧光寿命和荧光偏振以及双光子激光扫描显微镜。

该试剂是染料的活化 N-羟基琥珀酰亚胺酯，对伯氨基和仲氨基具有反应性。

BDP 576/589 NHS ester相关产品

BDP TR X NHS ester

BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used for the conjugation with proteins and peptides.
BDP TR X NHS 酯是 BDP TR 的衍生物，含有一个基于氨基己酸 (C6) 的长接头。该染料具有接近ROX的吸收和发射。 NHS 酯功能可用于与蛋白质和肽的结合。

Coumarin 343 X NHS ester

Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
香豆素 343 是一种蓝色发射染料，可用作荧光素 (FAM) 的 FRET 供体。这种结构的特点是香豆素 343 荧光团和官能团之间有一个接头。它提供了更好的溶解性，以及染料和官能团之间的空间分离。

FAM amine, 5-isomer

Amino derivative of fluorescein, pure 5-isomer. Contains a linker arm between the aliphatic amine group, and the fluorophore. Useful for the labeling via enzymatic transamination, for the reactions with electrophilic reagents, and for reductive amination.
荧光素的氨基衍生物，纯 5-异构体。在脂肪胺基团和荧光团之间包含一个连接臂。可用于通过酶促氨基转移进行标记、与亲电子试剂的反应以及还原胺化。

General properties

Appearance:	dark colored crystals
Mass spec M+ increment:	328.1
Molecular weight:	426.18
CAS number:	201998-61-0
Molecular formula:	C₂₀H₁₇N₄BF₂O₄
Solubility:	soluble in polar organic solvents
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	580
ε, L⋅mol⁻¹⋅cm⁻¹:	98000
Emission maximum, nm:	592
Fluorescence quantum yield:	0.13