- Products
- Categories
- Click chemistry
- endo-BCN CE-Phosphoramidite
endo-BCN CE-Phosphoramidite
| Cat. # | Quantity | Price | Lead time | |
|---|---|---|---|---|
| 3005-100mg | 100 mg | –  Sample request |
||
| 3005-250mg | 250 mg | 800.00$
|
||
| 3005-1g | 1 g | 2350.00$
|
Bicyclononyne (BCN) is a stable and one of the most reactive cyclooctynes for Copper-free click chemistry. Unlike dibenzocyclooctyne (DBCO), BCN is reactive both to azides (strain-promoted azyde-alkyne cycloaddition, SPAAC) and tetrazines (inverse electron demand Diels-Alder reaction, IEDDA). Being an endo-stereoisomer, Bicyclononine in endo-BCN CE-phosphoramidite provides a higher rate of cycloaddition compared to its exo-conformer.
BCN-labeled oligonucleotides may be used for the conjugation to azide- or tetrazine-containing solid surfaces, polymers, and large proteins.
Notes:
Coupling time is standard, like for amidites of natural nucleosides. Exclude the dimethoxytrityl (DMT) removal step and use the Dmt-ON protocol after amidite coupling and oxidation.
Use standard conditions for deprotection and ammonia solution, or AMA mixture (ammonium hydroxide / 40% methylamine, 1:1).
Customers also purchased with this product
Cyanine5 azide
Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.
Oligo synthesis details
| Diluent: | Anhydrous Acetonitrile |
| Deprotection conditions: | identical to protected nucleobases |
