标签： BCA-1/BLC 人重组

C-X-C motif chemokine 13, Small-inducible cytokine B13, B lymphocyte chemoattractant, CXC chemokine BLC, CXCL13, BCA1, BCA-1, CXCL-13, B cell Attracting Chemokine-1, BLC, ANGIE, BLR1L, SCYB13, ANGIE2.

BCA-1 is a CXC chemokine that is highly expressed in thesecondary lymphoid organs, such as follicles of the spleen, lymph nodes, and Peyer’s patches. CXCL13 promotes the migration of B lymphocytes , by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt’s lymphoma receptor 1 . BCA1 therefore function in the homing of B lymphocytes to follicles. Human BCA-1 shares a 64% amino acid sequence similarity with the mouse protein and 23 – 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA1. Among cells of the hematopoietic lineages, the expression of BLR-1, now designated CXCR-5, is restricted to B lymphocytes and a subpopulation of T helper memory cells.

BCA-1是一种CXC趋化因子，在次级淋巴器官中高度表达，例如脾脏、淋巴结和派尔氏斑的滤泡。 CXCL13 通过刺激钙流入并趋化表达伯基特淋巴瘤受体 1 的细胞，从而促进 B 淋巴细胞的迁移。 因此，BCA1 在 B 淋巴细胞归巢到滤泡中起作用。 人 BCA-1 与小鼠蛋白质具有 64% 的氨基酸序列相似性，与其他已知的 CXC 趋化因子具有 23-34% 的氨基酸序列同一性。 重组或化学合成的 BCA1 对 B 淋巴细胞是一种有效的化学引诱物，但对 T 淋巴细胞、单核细胞或中性粒细胞没有。 BLR1 是一种 G 蛋白偶联受体，最初是从 Burkitt 淋巴瘤细胞中分离出来的，现在已被证明是 BCA1 的特异性受体。 在造血谱系的细胞中，BLR-1（现在称为 CXCR-5）的表达仅限于 B 淋巴细胞和 T 辅助记忆细胞的一个亚群。

在大肠杆菌中产生的 CXCL13 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 87 个氨基酸，分子量为 10.3 kDa。
BCA-1 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The BCA-1 protein was lyophilized from a concentrated solution containing 20mM PBS & 150mM NaCl pH-7.4.

It is recommended to reconstitute the lyophilized CXCL13 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized BCA1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BCA1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

VLEVYYTSLRCRCVQESSVFIPRRFIDRIQILPRGNG CPRKEIIVWKKNKSIVCVDPQAEWIQRMMEVLRKR SSSTLPVPVFKRKIP.

Determined by its ability to chemoattract human B cells using a concentration range of 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.