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• Sulfo-Cyanine5-PEG3-Biotin

Sulfo-Cyanine5-PEG3-Biotin

Sulfo-Cyanine5 is a far-red fluorophore widely used for biomolecule labeling, with excitation maximum at 646 nm and emission maximum at 662 nm. Far-red fluorescent tags with excitation above 600 nm and emission further than 650 nm are valuable for imaging techniques because of the lower background autofluorescence at these wavelengths. Besides, far-red fluorescent labels can be imaged simultaneously with near-red, orange, green, and blue tags, which is advantageous for multicolor imaging.

Biotin, or the water-soluble vitamin H, is well known not only for playing essential roles in various crucial metabolic cellular reactions but also for its extremely high affinity for avidin, a glycoprotein of egg white. Avidin, streptavidin (bacterial analogue of avidin), and neutravidin (deglycosylated avidin) bind non-cooperatively to biotin with high affinity. Thus, sulfo-Cyanine5 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin, and neutravidin in samples of different origin.

Sulfo-Cyanine5 biotin conjugate is a water-soluble reagent and its fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 linker between biotin moiety and fluorescent tag provides binding to avidin, streptavidin, or neutravidin without steric troubles.

The major applications of sulfo-Cyanine5 biotin conjugate include imaging (e.g. primary and secondary antibody labeling for Western Blotting, immunoassay, cyto- and histochemistry, flow cytometry), affinity and dissociation constant measuring, streptavidin-based sensors, etc.

Sulfo-Cyanine5 是一种远红荧光团，广泛用于生物分子标记，最大激发波长为 646 nm，最大发射波长为 662 nm。激发波长高于 600 nm 且发射波长超过 650 nm 的远红荧光标签对成像技术很有价值，因为这些波长的背景自发荧光较低。此外，远红荧光标签可以与近红、橙、绿、蓝标签同时成像，有利于多色成像。

众所周知，生物素或水溶性维生素 H 不仅在各种关键的代谢细胞反应中发挥重要作用，而且还因其对抗生物素蛋白（一种蛋清的糖蛋白）具有极高的亲和力。抗生物素蛋白、链霉抗生物素蛋白（抗生物素蛋白的细菌类似物）和中性抗生物素蛋白（去糖基化的抗生物素蛋白）以高亲和力与生物素非协同结合。因此，磺基-Cyanine5 生物素偶联物可用于检测和量化不同来源样品中抗生物素蛋白、链霉抗生物素蛋白和中性抗生物素蛋白的生物素结合位点。

Sulfo-Cyanine5 生物素偶联物是一种水溶性试剂，其荧光在 pH 4 到 pH 10 之间不受 pH 影响。生物素部分和荧光标签之间的灵活 PEG3 接头可与抗生物素蛋白、链霉抗生物素蛋白或中性抗生物素蛋白结合，而不会出现空间问题。

磺基-Cyanine5 生物素偶联物的主要应用包括成像（例如用于蛋白质印迹、免疫测定、细胞和组织化学、流式细胞术的一抗和二抗标记）、亲和力和解离常数测量、基于链霉亲和素的传感器等。