AF430 tetrazine

AF430 tetrazine,AF430 是一种荧光染料。 AF430 在 430 nm 处具有最大激发,在 542 nm 处具有最大发射。 AF430 是为数不多的在 400 nm 和 450 nm 之间吸收的染料之一。 AF430 荧光在广泛的 pH 值范围内具有光稳定性和 pH 不敏感性。

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  • AF430 tetrazine

AF430 tetrazine

标签:Tetrazine, Microscopy, AF430
货号 规格 价格 货期
108E0 1 mg 110.00$ in stock
208E0 5 mg 290.00$ in stock
308E0 10 mg 460.00$ in stock
408E0 25 mg 690.00$ in stock
508E0 50 mg 1270.00$ in stock
608E0 100 mg 1990.00$ in stock

AF430 is a fluorescent dye. AF430 has an excitation maximum at 430 nm and an emission maximum at 542 nm. AF430 is one of the few dyes that absorb between 400 nm and 450 nm. AF430 fluorescence is photostable and pH-insensitive in a broad range of pH values.

Tetrazine residue participates in the fast copper-free click reaction that is well suited to non-toxic in vitro cell labeling and low-concentration applications.

AF430 是一种荧光染料。 AF430 在 430 nm 处具有最大激发,在 542 nm 处具有最大发射。 AF430 是为数不多的在 400 nm 和 450 nm 之间吸收的染料之一。 AF430 荧光在广泛的 pH 值范围内具有光稳定性和 pH 不敏感性。

四嗪残基参与快速无铜点击反应,非常适合无毒的体外细胞标记和低浓度应用。

Absorption and emission spectra of AF430

AF430 tetrazine

AF430 tetrazine相关产品

Cyanine5 tetrazine

Cyanine5 is a universal red emitting dye with a very high extinction coefficient, and good quantum yield. This tetrazine derivative reacts very quickly with trans-cyclooctenes and cyclopropenes with the formation of stable conjugates.

Cy5 是一种通用的红色发光染料,具有非常高的消光系数和良好的量子产率。 这种四嗪衍生物与反式环辛烯和环丙烯反应非常迅速,形成稳定的共轭物。

AmdU (5-azidomethyl-2′-deoxyuridine)

Azidomethyldeoxyuridine (AmdU) is an azide containing nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain promoted Click chemistry.

叠氮甲基脱氧尿苷 (AmdU) 是一种含有核苷的叠氮化物,可通过细胞聚合酶整合到新生 DNA 中。 以这种方式连接到 DNA 上的叠氮基可以随后通过铜催化或应变促进的点击化学被炔烃或环炔烃修饰。

BDP FL tetrazine

BDP FL tetrazine (methyltetrazine) is a reactive dye for the inverse electron demand Diels-Alder (ied-DA) reaction with trans-cyclooctenes, cyclopropenes, and other strained cycloolefines. BDP FL is a bright and photostable dye for the 488 nm fluorescein (FAM) channel.

BDP FL 四嗪(甲基四嗪)是一种活性染料,用于与反式环辛烯、环丙烯和其他应变环烯烃进行逆电子需求 Diels-Alder (ied-DA) 反应。 BDP FL 是一种用于 488 nm 荧光素 (FAM) 通道的明亮且光稳定的染料。

General properties

Appearance: dark orange solid 深橙色固体
Mass spec M+ increment: 658.2
Molecular weight: 724.79
Molecular formula: C32H32N6F3KO6S
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 430
ε, L⋅mol−1⋅cm−1: 15955
Emission maximum, nm: 542
Fluorescence quantum yield: 0.23
CF260: 0.06
CF280: 0.06

AF430 amine

AF430 amine,AF430 是一种在宽 pH 范围(4 到 10)内的稳定染料,其荧光峰位于黄绿色区域(542 nm)。

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  • AF430 amine

AF430 amine

标签:Amines, AF430
货号 规格 价格 货期
108C0 1 mg 110.00$ in stock
208C0 5 mg 290.00$ in stock
408C0 25 mg 690.00$ in stock
508C0 50 mg 1270.00$ in stock
608C0 100 mg 1990.00$ in stock

AF430 is a stable dye over a broad pH range (from 4 to 10) with the fluorescence peak in the yellow-green region (at 542 nm).

Conjugates of biomolecules with AF430 can be used in flow cytometry, for example in multicolor staining. AF430 is also used in cell microscopy due to its high photostability.

AF430 amine is soluble in water and can be conjugated with electrophiles and participate in enzymatic transamination.

AF430 是一种在宽 pH 范围(4 到 10)内的稳定染料,其荧光峰位于黄绿色区域(542 nm)。

生物分子与 AF430 的偶联物可用于流式细胞术,例如多色染色。 由于其高光稳定性,AF430 也用于细胞显微镜。

AF430 胺可溶于水,可与亲电试剂结合并参与酶促转氨作用。

Absorption and emission spectra of AF430

AF430 amine

AF430 amine相关产品

AF430 NHS ester

AF430 is a hydrophilic coumarin dye with the emission in green-yellow range. The NHS ester is used for the labeling of amine groups.

AF430 是一种亲水性香豆素染料,发射在黄绿色范围内。 NHS 酯用于标记胺基。

AF430 carboxylic acid

Non-reactive form of AF430 fluorescent dye to be used as a reference standard

AF430 荧光染料的非反应性形式用作参考标准

AF430 alkyne

AF430 is a dye emitting in green-yellow range. A dye is useful for flow cytometry.

AF430 是一种在黄绿色范围内发光的染料。 染料可用于流式细胞术。

General properties

Appearance: yellow solid 黄色固体
Molecular weight: 601.68
Molecular formula: C28H38N3F3O6S
IUPAC name: (9-(6-((6-ammoniohexyl)amino)-6-oxohexyl)-8,8-dimethyl-2-oxo-4-(trifluoromethyl)-8,9-dihydro-2H-pyrano[3,2-g]quinolin-6-yl)methanesulfonate
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 430
ε, L⋅mol−1⋅cm−1: 15955
Emission maximum, nm: 542
Fluorescence quantum yield: 0.23
CF260: 0.06
CF280: 0.06

AF430 carboxylic acid

AF430 carboxylic acid,AF430 的激发最大值为 430 nm,发射最大值为 542 nm,斯托克斯位移为 112 nm。 AF430 表现出高光稳定性和对 pH 不敏感的荧光。 染料可以被 405 nm 紫激光或 445 nm 激光激发。

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  • AF430 carboxylic acid

AF430 carboxylic acid

标签:Carboxylic acids Microscopy, AF430
货号 规格 价格 货期
10890 1 mg 110.00$ in stock
20890 5 mg 165.00$ in stock
40890 25 mg 380.00$ in stock
50890 50 mg 695.00$ in stock
60890 100 mg 1190.00$ in stock

AF430’s excitation maximum is at 430 nm, emission maximum is at 542 nm, and Stokes shift is 112 nm. AF430 exhibits high photostability and pH-insensitive fluorescence. The dye can be excited by 405 nm violet laser or 445 nm laser.

AF430 carboxylic acid is a non-reactive form of AF430 dye that can be used for the analysis of labeled samples — as a reference standard in procedures where AF430 dye conjugates are involved.

AF430 的激发最大值为 430 nm,发射最大值为 542 nm,斯托克斯位移为 112 nm。 AF430 表现出高光稳定性和对 pH 不敏感的荧光。 染料可以被 405 nm 紫激光或 445 nm 激光激发。

AF430 羧酸是一种非反应性形式的 AF430 染料,可用于分析标记样品——在涉及 AF430 染料偶联物的程序中作为参考标准。

Absorption and emission spectra of AF430

AF430 carboxylic acid

AF430 carboxylic acid相关产品

BDP TMR carboxylic acid

Borondipyrromethene dye for TMR channel with a non-activated carboxylic acid group.

用于 TMR 通道的硼二吡咯亚甲基染料,具有未活化的羧酸基团。

AF430 maleimide

AF430 is a green-yellow emitting fluorescent dye of coumarin series. This maleimide derivative allows to label thiolated biomolecules.

AF430是香豆素系列的绿-黄发光荧光染料。 这种马来酰亚胺衍生物可以标记硫醇化的生物分子。

AF430 alkyne

AF430 is a dye emitting in green-yellow range. A dye is useful for flow cytometry.

AF430 是一种在黄绿色范围内发光的染料。 染料可用于流式细胞术。

General properties

Appearance: yellow solid 黄色固体
Molecular weight: 541.58
Molecular formula: C22H23NF3KO7S
Solubility: good in DMF, DMSO, water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 430
ε, L⋅mol−1⋅cm−1: 15955
Emission maximum, nm: 542
Fluorescence quantum yield: 0.23
CF260: 0.06
CF280: 0.06

AF430 maleimide

AF430 maleimide,AF430 是香豆素性质的亲水染料。 该染料用于流式细胞术。这种马来酰亚胺衍生物对硫醇基团具有反应性。 因此,它可以标记许多蛋白质,包括那些驻留在细胞表面的蛋白质。

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  • AF430 maleimide

AF430 maleimide

标签:Maleimides, AF430
货号 规格 价格 货期
10880 1 mg 110.00$ in stock
20880 5 mg 290.00$ in stock
40880 25 mg 690.00$ in stock
50880 50 mg 1270.00$ in stock
60880 100 mg 1990.00$ in stock

AF430 is a hydrophilic dye of coumarin nature. The dye is used in flow cytometry.

This maleimide derivative is reactive against thiol groups. Therefore, it allows to label many proteins, including those residing on cell surface.

AF430 是香豆素性质的亲水染料。 该染料用于流式细胞术。

这种马来酰亚胺衍生物对硫醇基团具有反应性。 因此,它可以标记许多蛋白质,包括那些驻留在细胞表面的蛋白质。

Absorption and emission spectra of AF430

AF430 maleimide

AF430 maleimide相关产品

Sulfo-Cyanine5 NHS ester

Water-soluble Cyanine 5 succinimide ester.

水溶性Cy5琥珀酰亚胺酯。

TAMRA NHS ester, 5-isomer

Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides.

TAMRA 染料的胺反应活性 N-羟基琥珀酰亚胺酯,用于标记蛋白质、肽和氨基寡核苷酸的胺基。

Sulfo-Cyanine3 azide

Water-soluble sulfo-Cyanine3 dye azide for Click Chemistry.

用于 Click Chemistry 的水溶性磺基-Cyanine3 染料叠氮化物。

General properties

Appearance: yellow solid 黄色固体
Mass spec M+ increment: 625.2
Molecular weight: 726.8
Molecular formula: C34H45N4F3O8S
IUPAC name: (8-{6-[2-(2,5-Dioxo-1H-pyrrol-1-yl)ethylamino]-6-oxohexyl}-7,7-dimethyl-2-oxo-4-(trifluoromethyl)-1-oxa-8-aza-5,6,7,8-tetrahydroanthr-5-yl)methanesulfonic acid
Solubility: soluble in water, polar organic solvents (DMF, DMSO)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 430
ε, L⋅mol−1⋅cm−1: 15955
Emission maximum, nm: 542
Fluorescence quantum yield: 0.23
CF260: 0.06
CF280: 0.06

AF430 alkyne,AF430 炔烃

AF430 alkyne,AF430 炔烃,AF430 是一种磺化香豆素染料,可用于流式细胞术和抗体标记。 荧光在很宽的 pH 值范围内是恒定的。

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  • AF430 alkyne

AF430 alkyne

标签:Alkynes, AF430,炔烃,Dye alkynes
货号 规格 价格 货期
A08B0 1 mg 110.00$ in stock
B08B0 5 mg 290.00$ in stock
C08B0 10 mg 470.00$ in stock
D08B0 25 mg 690.00$ in stock
E08B0 50 mg 1270.00$ in stock
F08B0 100 mg 1990.00$ in stock

AF430 is a sulfonated coumarin dye that is useful for flow cytometry and antibody labeling. The fluorescence is constant in wide range of pH values.

This particular derivative contains terminal alkyne group that is suitable for copper catalyzed CuAAc reaction.

AF430 是一种磺化香豆素染料,可用于流式细胞术和抗体标记。 荧光在很宽的 pH 值范围内是恒定的。

这种特殊的衍生物含有适合铜催化的 CuAAc 反应的末端炔基。

Absorption and emission spectra of AF430

AF430 alkyne

Customers also purchased with this product

R6G alkyne, 6-isomer

R6G (rhodamine 6G) terminal alkyne for copper catalyzed Click chemistry reaction with azides. Pure 6-isomer of the dye.

R6G(罗丹明 6G)末端炔烃用于铜催化与叠氮化物的点击化学反应。 染料的纯 6-异构体。

AF430 carboxylic acid

Non-reactive form of AF430 fluorescent dye to be used as a reference standard

AF430 荧光染料的非反应性形式用作参考标准

AF430 tetrazine

Tetrazine derivative of AF430 fluorescent dye suitable for in vitro cell labeling.

AF430 荧光染料的四嗪衍生物,适用于体外细胞标记。

General properties

Appearance: orange solid 橙色固体
Molecular weight: 578.64
Molecular formula: C25H26N2F3KO6S
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 430
ε, L⋅mol−1⋅cm−1: 15955
Emission maximum, nm: 542
Fluorescence quantum yield: 0.23
CF260: 0.06
CF280: 0.06

AF430 azide

AF430 azide,AF430 是一种荧光染料。 它可以被 405 nm 紫激光或 445 nm 激光与 510/80 nm 带通滤光片配对激发。

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  • AF430 azide

AF430 azide

标签:Azides AF430
价格
货号 规格 货期
A0830 1 mg 110.00$ in stock
B0830 5 mg 290.00$ in stock
D0830 25 mg 690.00$ in stock
E0830 50 mg 1270.00$ in stock
F0830 100 mg 1990.00$ in stock

AF430 is a fluorescent dye. It can be excited by 405 nm violet laser or 445 nm laser paired, for example, with 510/80 nm bandpass filter.

Azide moiety allows quick and effective labeling and detection of terminal alkynes via a copper-catalyzed click reaction (CuAAC) or of strained cyclooctynes via a copper-free click chemistry reaction. Mild reaction conditions are suitable for most biomolecules, cells, and tissues. The product is water soluble and insensitive to pH changes between pH 4 and pH 10.

AF430 是一种荧光染料。 它可以被 405 nm 紫激光或 445 nm 激光与 510/80 nm 带通滤光片配对激发。

叠氮化物部分允许通过铜催化的点击反应 (CuAAC) 快速有效地标记和检测末端炔烃,或通过无铜点击化学反应检测应变的环辛炔。 温和的反应条件适用于大多数生物分子、细胞和组织。 该产品是水溶性的,对 pH 4 和 pH 10 之间的 pH 变化不敏感。
AF430 azide AF430 azide AF430 azide AF430 azide

Absorption and emission spectra of AF430

AF430 azide

AF430 azide相关产品

AF568 NHS ester

AF568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.

AF568 衍生物,用于用游离胺标记蛋白质和其他高分子量和低分子量配体。

AF430 maleimide

AF430 is a green-yellow emitting fluorescent dye of coumarin series. This maleimide derivative allows to label thiolated biomolecules.

AF430是香豆素系列的绿-黄发光荧光染料。 这种马来酰亚胺衍生物可以标记硫醇化的生物分子。

AF430 carboxylic acid

Non-reactive form of AF430 fluorescent dye to be used as a reference standard

AF430 荧光染料的非反应性形式用作参考标准

General properties

Appearance: yellow solid
Molecular weight: 623.69
Molecular formula: C25H29N5F3KO6S
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 430
ε, L⋅mol−1⋅cm−1: 15955
Emission maximum, nm: 542
Fluorescence quantum yield: 0.23
CF260: 0.06
CF280: 0.06