AF568 azide, 6-isomer荧光染料

AF568 azide, 6-isomer,AF568 是一种荧光染料。 其激发峰位于 572 nm,发射峰位于 598 nm。 AF568 叠氮化物是一种光化学稳定、特异性和高效的生物分子标记工具。 它是水溶性的,对 pH 4 和 pH 10 之间的 pH 变化不敏感。反应条件不需要高温或高压。

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  • AF568 azide, 6-isomer

AF568 azide, 6-isomer

标签:Azides Microscopy AF568
货号 规格 价格 货期
A5820 1 mg 110.00$ in stock
B5820 5 mg 290.00$ in stock
C5820 10 mg 470.00$ in stock
D5820 25 mg 690.00$ in stock
E5820 50 mg 1270.00$ in stock
F5820 100 mg 1990.00$ in stock

AF568 is a fluorescent dye. Its excitation peak is at 572 nm and its emission peak at 598 nm. AF568 azide is a photochemically stable, specific, and highly efficient tool for labeling biomolecules. It is water soluble and insensitive to pH changes between pH 4 and pH 10. The reaction conditions do not require high temperature or pressure.

Labeling with AF568 azide via Click Chemistry is a powerful technique for the production of bioconjugates. Thus, AF568 azide is an excellent tool for imaging purposes, including fluorescent microscopy and flow cytometry, where label brightness and photostability are essential.

AF568 是一种荧光染料。 其激发峰位于 572 nm,发射峰位于 598 nm。 AF568 叠氮化物是一种光化学稳定、特异性和高效的生物分子标记工具。 它是水溶性的,对 pH 4 和 pH 10 之间的 pH 变化不敏感。反应条件不需要高温或高压。

通过 Click Chemistry 使用 AF568 叠氮化物进行标记是一种用于生产生物偶联物的强大技术。 因此,AF568 叠氮化物是用于成像目的的出色工具,包括荧光显微镜和流式细胞术,其中标签亮度和光稳定性至关重要。

AF568 azide, 6-isomer AF568 azide, 6-isomer AF568 azide, 6-isomer AF568 azide, 6-isomer

Absorption and emission spectra of AF568

AF568 azide, 6-isomer

AF568 azide, 6-isomer相关产品

AF488 NHS ester

AF488 NHS ester, a bright, photostable and hydrophilic fluorescent dye for green channel.
AF488 NHS 酯,一种用于绿色通道的明亮、光稳定性和亲水性荧光染料。

AF568 carboxylic acid

Non-reactive form of AF568 fluorescent dye
AF568 荧光染料的非反应性形式

AF488 azide

AF488 azide is a fluorophore with emission in the green spectrum range. It reacts with alkynes in copper-catalyzed cycloaddition reactions.
AF488 azide 是一种在绿色光谱范围内发射的荧光团。 它在铜催化的环加成反应中与炔烃反应。

General properties

Appearance: dark colored solid
Mass spec M+ increment: 776.2
Molecular weight: 853.02
Molecular formula: C36H34N6K2O10S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 572
ε, L⋅mol−1⋅cm−1: 94238
Emission maximum, nm: 598
Fluorescence quantum yield: 0.912
CF260: 0.4
CF280: 0.32

lumiprobe -FAM NHS ester, 6-isomer

FAM NHS ester, 6-isomer,荧光素 (FAM) 是一种明亮的荧光团,在生命科学研究中有着极其广泛的应用。 荧光团是亲水性的,其衍生物在水性缓冲液中具有良好的溶解性。

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  • FAM NHS ester, 6-isomer

FAM NHS ester, 6-isomer

标签:FAM (Fluorescein) NHS esters  Fluorescence  polarization  Microscopy  Protein labeling
货号 规格 价格 货期
25120 5 mg in stock
45120 25 mg 140.00$ in stock

55120 50 mg 240.00$ in stock
65120 100 mg 370.00$ in stock
85120 1 g please inquire in stock
95120 5 g please inquire in stock  

Fluorescein (FAM) is a bright fluorophore that has found an extremely wide application in life science research. The fluorophore is hydrophilic and its derivatives possess good solubility in aqueous buffers.

Fluorescein is compatible with a wide variety of fluorescent instrumentation – most instruments are equipped with FAM channel filters.

This product contains the pure 6-isomer of fluorescein.

荧光素 (FAM) 是一种明亮的荧光团,在生命科学研究中有着极其广泛的应用。 荧光团是亲水性的,其衍生物在水性缓冲液中具有良好的溶解性。

荧光素与多种荧光仪器兼容——大多数仪器都配备了 FAM 通道过滤器。

本产品含有纯6-异构体的荧光素。

FAM NHS ester, 6-isomer相关产品

BDP TR X NHS ester

BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used for the conjugation with proteins and peptides.

BDP TR X NHS 酯是 BDP TR 的衍生物,含有一个基于氨基己酸 (C6) 的长接头。 该染料具有接近ROX的吸收和发射。 NHS 酯功能可用于与蛋白质和肽的结合。

BDP FL NHS ester

Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.

氨基反应性 BDP FL,一种非常明亮且光稳定的荧光素通道染料。

Sulfo-Cyanine5 amine

Sulfonated Cyanine5 amine derivative, for the conjugation with electrophiles, and enzymatic transamination labeling.

磺化的 Cyanine5 胺衍生物,用于与亲电试剂偶联和酶促转氨标记。

General properties

Appearance: yellow crystalline solid
Mass spec M+ increment: 358.0
Molecular weight: 473.39
CAS number: 92557-81-8
Molecular formula: C25NO9H15
IUPAC name: (2,5-dioxopyrrolidin-1-yl) 3′,6′-dihydroxy-1-oxospiro[2-benzofuran-3,9′-xanthene]-5-carboxylate
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

参考文献:

  1. Sasaki, K.; Harada, M.; Yoshikawa, T.; Tagawa, H.; Harada, Y.; Yonemitsu, Y.; Ryujin, T.; Kishimura, A.; Mori, T.; Katayama, Y. Fc-Binding Antibody-Recruiting Molecules Targeting Prostate-Specific Membrane Antigen: Defucosylation of Antibody for Efficacy Improvement. ChemRxiv, preprint. doi: 10.26434/chemrxiv.12654602.v1
  2. Zhong, Z.; Deng, X.; Wang, P.; Yu, C.; Kiratitanaporn, W.; Wu, X.; Schimelman, J.; Tang, M.; Balayan, A.; Yao, E.; Tian, J.; Chen, L.; Zhang, K.; Chen, S. Rapid bioprinting of conjunctival stem cell micro-constructs for subconjunctival ocular injection. Biomaterials, 2021, 267, 120462. doi: 10.1016/j.biomaterials.2020.120462
  3. Iurova, E.; Beloblov, I.; Beloborodov, E.; Rastorgueva, E.; Pogodina, E.; Tazintseva, E.; Saenko, Y.; Fomin, A. Use of Natural Peptides with a Cysteine Knot of Arthropods as a Carrier of a Peptide Tropic to Glutamate Carboxypeptidase II. Systematic Reviews in Pharmacy, 2020, 11(9), 503–509. doi: 10.31838/srp.2020.9.73
  4. Pinals, R.L.; Yang, D.; Lui, A.; Cao, W.; Landry, M.P. Corona exchange dynamics on carbon nanotubes by multiplexed fluorescence monitoring. Journal of the American Chemical Society, 2020, 142(3), 1254–1264. doi: 10.1021/jacs.9b09617

R110 azide, 6- isomer

R110 azide, 6- isomer,R110(罗丹明 110)叠氮化物,在 DMSO 和固体材料中的 10 mM 溶液,用于点击化学的标记试剂。 纯 6 异构体。与荧光素光物理性质相似的染料,但更耐光。 可替代 DyLight 488、FAM(荧光素)。

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  • R110 azide, 6- isomer

R110 azide, 6- isomer

货号 规格 价格 货期
15230 100 uL, 10 mM/DMSO 160.00$ in stock
35230 500 uL, 10 mM/DMSO 420.00$ in stock
45230 1 mL, 10 mM/DMSO 595.00$ in stock
A5230 1 mg 110.00$ in stock
D5230 25 mg 410.00$ in stock
E5230 50 mg 695.00$ in stock

R110 (rhodamine 110) azide, 10 mM solution in DMSO and solid material, labeling reagent for Click chemistry. Pure 6-isomer.

Dye of similar photophysical properties with fluorescein, but more photostable. Can replace DyLight 488, FAM (fluorescein).

R110(罗丹明 110)叠氮化物,在 DMSO 和固体材料中的 10 mM 溶液,用于点击化学的标记试剂。 纯 6 异构体。

与荧光素光物理性质相似的染料,但更耐光。 可替代 DyLight 488、FAM(荧光素)。

R110 azide, 6- isomer相关产品

Coumarin 343 X azide

Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.

Coumarin 343 是一种蓝色荧光团,可作为荧光素 (FAM) 的 FRET 供体。 这种叠氮化物衍生物可以通过点击化学与炔烃结合。 荧光团和叠氮化物基团之间的延伸连接臂增强了溶解度,并提供了染料和生物分子之间的空间分离。

Cyanine3 DBCO

Cyanine3 dibenzocyclooctyne (DBCO, or ADIBO) is a cycloalkyne dye derivative for copper free Click chemistry.

Cyanine3 dibenzocyclooctyne (DBCO, or ADIBO) 是一种用于无铜点击化学的环炔烃染料衍生物。

General properties

Appearance: violet powder / yellow solution 紫色粉末/黄色溶液
Molecular weight: 456.45
CAS number: 1622395-29-2
Molecular formula: C24H20N6O4
Solubility: soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 496
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9

相关文献:

  1. Shieh, P.; Siegrist, M.S.; Cullen, A.J.; Bertozzi, C.R. Imaging bacterial peptidoglycan with near-infrared fluorogenic azide probes. Proceedings of the National Academy of Sciences of the U.S.A., 2014, 111(15), 5456–5461. doi: 10.1073/pnas.1322727111