lumiprobe BDP 染料 BDP TMR Ceramide lumiprobe代理

lumiprobe,BDP TMR Ceramide,BDP TMR 神经酰胺,活性染料,lumiprobe代理,这种鞘氨醇衍生物 BDP TR 神经酰胺是一种分子,旨在在共聚焦显微镜下可视化活细胞的高尔基体。 染料在橙色区域发射。

lumiprobe BDP 染料 BDP TMR Ceramide lumiprobe代理

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  • BDP TMR Ceramide

BDP TMR Ceramide

BDP TMR, Microscopy
Cat. # Quantity Price Lead time
3058-250ug 250 ug 275.00$ 21 days

BDP dyes are well suited for microscopy of lipids due to their excellent photostability and inherent hydrophobicity.

This sphingosine derivative, BDP TR ceramide, is a molecule designed to visualize Golgi apparatus of live cells under confocal microscopy. The dye emits in orange region.

由于 BDP 染料具有出色的光稳定性和固有的疏水性,因此非常适合用于脂质显微镜检查。

这种鞘氨醇衍生物 BDP TR 神经酰胺是一种分子,旨在在共聚焦显微镜下可视化活细胞的高尔基体。 染料在橙色区域发射。

General properties

Appearance: red/brown semisolid
Molecular weight: 679.69
Molecular formula: C39H56BF2N3O4
Solubility: good in many organic solvents
Quality control: 95+% HPLC-MS and 1H NMR
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 542
ε, L⋅mol−1⋅cm−1: 55000
Emission maximum, nm: 574
Fluorescence quantum yield: 0.64
CF260: 0.16
CF280: 0.16

BDP TMR Ceramide

lumiprobe,BDP FL Ceramide,BDP FL 神经酰胺,活性染料,3057-250ug

lumiprobe,BDP FL Ceramide,BDP FL 神经酰胺,活性染料,3057-250ug,BDP FL 是一种发绿光的荧光染料,具有明亮、中等疏水性和高度光稳定性。 因此,它非常适合通过显微镜研究脂质。

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  • BDP FL Ceramide

BDP FL Ceramide

BDP FL, Microscopy
Cat. # Quantity Price Lead time
3057-250ug 250 ug 275.00$ 21 days
 

BDP FL is a green emitting fluorescent dye that is bright, moderately hydrophobic, and highly photostable. Therefore, it is ideally suitable for the study of lipides by means of microscopy.

This conjugate with sphingosine – BDP FL ceramide – is a derivative that is used for the visualization of Golgi apparatus membranes in fluorescent confocal microscopy.

BDP FL 是一种发绿光的荧光染料,具有明亮、中等疏水性和高度光稳定性。 因此,它非常适合通过显微镜研究脂质。

这种与鞘氨醇的结合物 – BDP FL 神经酰胺 – 是一种衍生物,用于在荧光共聚焦显微镜中显示高尔基体膜。

General properties

Appearance: green orange semisolid
Molecular weight: 573.57
Molecular formula: C32H50BF2N3O3
Solubility: good in many organic solvents
Quality control: 95+% HPLC-MS and 1H NMR
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 503
ε, L⋅mol−1⋅cm−1: 92000
Emission maximum, nm: 509
Fluorescence quantum yield: 0.97
CF260: 0.015
CF280: 0.027

BDP FL Ceramide

lumiprobe -Cyanine5.5 NHS ester

Cyanine5.5 NHS ester,Cyanine5.5 NHS 酯是一种活性染料,用于标记肽、蛋白质和寡核苷酸中的氨基,是 Cy5.5® NHS 酯的类似物。

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  • Dye NHS esters
  • Cyanine5.5 NHS ester

Cyanine5.5 NHS ester

标签:Cyanine5.5 NHS esters Microscopy Protein labeling
货号 规格 价格 货期
17020 1 mg 110.00$ in stock
27020 5 mg 210.00$ in stock
47020 25 mg 410.00$ in stock
57020 50 mg 695.00$ in stock
67020 100 mg 1190.00$ in stock

Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5® and DyLight 680.
Cyanine5.5 NHS 酯是一种活性染料,用于标记肽、蛋白质和寡核苷酸中的氨基,是 Cy5.5® NHS 酯的类似物。

Cy5.5 是一种远红(和近红外)发射染料,非常适合需要考虑背景荧光的荧光测量。 它也适用于体内近红外成像实验。

该试剂可替代 Cy5.5® 和 DyLight 680 的 NHS 酯。

Cyanine5.5 NHS ester Cyanine5.5 NHS ester Cyanine5.5 NHS ester

Cy5.5 absorbance and emission spectra

Cyanine5.5 NHS ester

Cyanine5.5 NHS ester相关产品

Alkyne-PEG3-COOH

Bifunctional linker with terminal alkyne function, and carboxylic acid moiety, CAS# 1415800-32-6.
具有末端炔烃功能和羧酸部分的双功能接头,CAS# 1415800-32-6。

Cyanine7 DBCO

NIR fluorescent dye functionalized with cyclooctyne moiety for copper free Click chemistry conjugation with azides.
用环辛炔部分功能化的 NIR 荧光染料,用于与叠氮化物的无铜点击化学缀合。

Cyanine3 hydrazide

Cyanine3 hydrazide is a carbonyl-reactive dye for Cy3 channel.
Cyanine3 hydrazide 是 Cy3 通道的羰基反应性染料。

General properties

Appearance: dark blue solid
Molecular weight: 767.66
CAS number: 2375105-86-3
Molecular formula: C44H46N3BF4O4
IUPAC name: 1H-​Benz[e]​indolium, 2-​[5-​(1,​3-​dihydro-​1,​1,​3-​trimethyl-​2H-​benz[e]​indol-​2-​ylidene)​-​1,​3-​pentadien-​1-​yl]​-​3-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​1,​1-​dimethyl-
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM, < 1 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 684
ε, L⋅mol−1⋅cm−1: 198000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03

  1. Zahid, M.; McCandless, K.; Mishra, S.; Stiltner, J.; Feldman, K.; Yagi, H.; Yurko, R.; Islam, K.; Brown, J.; Frizzell, R. Novel Lung Targeting Cell Penetrating Peptides as Vectors for Delivery of Therapeutics. Research Square, preprint. doi: 10.21203/rs.3.rs-1056707/v1
  2. Chung, I.J.; Moon, H.; Jeon, S.I.; Lee, H.J.; Ahn, C.-H. Ultrasound-triggered imaging and drug delivery using microbubble-self-aggregate complexes. Journal of Biomaterials Science, Polymer Edition, 2022, 33(1), 57–76. doi: 10.1080/09205063.2021.1976362
  3. Guo, H.; Liu, L.; Hu, Q.; Dou, H. Monodisperse ZIF-8@dextran nanoparticles co-loaded with hydrophilic and hydrophobic functional cargos for combined near-infrared fluorescence imaging and photothermal therapy. Acta Biomaterialia, 2022, 137, 290–304. doi: 10.1016/j.actbio.2021.10.006
  4. Stepanova, I.A.; Lebedeva, A.N.; Shik, A..V.; Skorobogatov, E.V.; Beklemishev, M.K. Recognition and Determination of Sulfonamides by Near-IR Fluorimetry Using Their Effect on the Rate of the Catalytic Oxidation of a Carbocyanine Dye by Hydrogen Peroxide. Journal of Analytical Chemistry, 2021, 76(12), 1399–1407. doi: 10.1134/S1061934821120121

lumiprobe -Cyanine5 NHS ester

Cyanine5 NHS ester,Cyanine5 荧光团与各种仪器兼容,包括许多荧光显微镜、成像仪、扫描仪和荧光阅读器。存在许多不同的 Cyanine5 类似物 – Cyanine5 NHS 酯可以替代 Cy5® 和 DyLight 649 的活化酯。

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  • Cyanine5 NHS ester

Cyanine5 NHS ester

标签:Cyanine5 NHS esters Microscopy Protein labeling
货号 Quantity Price Lead time
13020 1 mg 110.00$ in stock
23020 5 mg 210.00$ in stock
43020 25 mg 410.00$ in stock
53020 50 mg 695.00$ in stock
63020 100 mg 1190.00$ in stock

During the last years, Cyanine5 (an analog of Cy5®) has become an incredibly popular label in life science research and diagnostics. The fluorophore has its emission maximum in the red region, where many CCD detectors exhibit maximum sensitivity, and biological objects show low background. The dye color is very intense, therefore quantities as small as 1 nmol can be detected in gel electrophoresis by naked eye.

This Cyanine5 NHS ester (analog to Cy5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties.

Cyanine5 fluorophore is compatible with various instrumentation including many fluorescent microscopes, imagers, scanners, and fluorescence readers. A number of various Cyanine5 analogs exist – Cyanine5 NHS ester can replace activated esters of Cy5® and DyLight 649.

在过去的几年里,Cyanine5(Cy5® 的类似物)已成为生命科学研究和诊断领域非常受欢迎的标签。荧光团在红色区域具有最大发射,许多 CCD 检测器在该区域表现出最大灵敏度,而生物物体则表现出低背景。染料颜色非常强烈,因此在凝胶电泳中肉眼可以检测到小至 1 nmol 的量。

这种 Cyanine5 NHS 酯(类似于 Cy5® NHS 酯)是一种活性染料,用于标记肽、蛋白质和寡核苷酸中的氨基。这种染料需要少量有机助溶剂(如 DMF 或 DMSO)用于标记反应(请参阅我们推荐的方案了解更多详细信息)。该试剂非常适合用于标记可溶性蛋白质以及各种肽和寡核苷酸的极具成本效益的方法。该试剂在有机溶剂中也适用于小分子标记。对于更复杂的目标,例如易于降解的蛋白质,当不希望使用 DMF 或 DMSO 时,请考虑使用不需要任何助溶剂且具有非常相似荧光特性的水溶性磺基花青 5 NHS 酯。

Cyanine5 荧光团与各种仪器兼容,包括许多荧光显微镜、成像仪、扫描仪和荧光阅读器。存在许多不同的 Cyanine5 类似物 – Cyanine5 NHS 酯可以替代 Cy5® 和 DyLight 649 的活化酯。

Cyanine5 NHS ester Cyanine5 NHS ester Cyanine5 NHS ester Cyanine5 NHS ester

Cy5 excitation and emission spectra

Cyanine5 NHS ester

Cyanine5 NHS ester相关产品

Cyanine7 amine

Cy7 amine is a NIR dye with free amine group.

Cy7 胺是一种带有游离胺基的 NIR 染料。

BDP TMR NHS ester

Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.

BDP TMR 的胺活性 NHS 酯,用于 TAMRA 通道的明亮硼二吡咯亚甲基染料,特别适用于荧光偏振测定。

BDP R6G azide

BDP R6G is a high performance borondipyrromethene fluorophore which is tuned to match excitation and emission channels of R6G dye.

BDP R6G 是一种高性能硼二吡咯亚甲基荧光团,经过调整以匹配 R6G 染料的激发和发射通道。

General properties

Appearance: dark blue solid
Molecular weight: 667.54
CAS number: 1263093-76-0
Molecular formula: C36H42N3BF4O4
IUPAC name: 3H-​Indolium, 2-​[5-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​2H-​indol-​2-​ylidene)​-​1,​3-​pentadien-​1-​yl]​-​1-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​3,​3-​dimethyl-​, tetrafluoroborate
Solubility: very poorly soluble in water (0.19 mM = 127 mg/L), good in polar (DMSO, DMF) and chlorinated (DCM, chloroform) organic solvents
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

  1. Gruenke, P.R.; Aneja, R.; Welbourn, S.; Ukah, O.B.; Sarafianos, S.G.; Burke, D.H.; Lange, M.J. Selection and identification of an RNA aptamer that specifically binds the HIV-1 capsid lattice and inhibits viral replication. Nucleic Acids Research, in press. doi: 10.1093/nar/gkab1293
  2. Janiszewski, T.; Kołt, S.; Kaiserman, D.; Snipas, S.; Li, S.; Kulbacka, J.; Saczko, J.; Bovenschen, N.; Salvesen, G.; Drąg, M.; Bird, P.I.; Kasperkiewicz, P. Noninvasive optical detection of Granzyme B from natural killer cells using enzyme-activated fluorogenic probes. bioRxiv, preprint. doi: 10.1101/2019.12.16.875070
  3. Poreba, M.; Groborz, K.; Rut, W.; Pore, M.; Snipas, S.J.; Vizovisek, M.; Turk, B.; Kuhn, P.; Drag, M.; Salvesen, G.S. The Activome: multiplexed probing of activity of proteolytic enzymes using mass cytometry-compatible activity-based probes (TOF-probes). bioRxiv, preprint. doi: 10.1101/775627
  4. Mu, Q.; Lin, G.; Jeon, M.; Wang, H.; Chang, F.-C.; Revia, R.A.; Yu, J.; Zhang, M. Iron oxide nanoparticle targeted chemo-immunotherapy for triple negative breast cancer. Materials Today, 2021, 50, 149–169. doi: 10.1016/j.mattod.2021.08.002

lumiprobe -Coumarin 343 X NHS ester

Coumarin 343 X NHS ester,蓝色发射香豆素 343 染料可与荧光素 (FAM) 形成 FRET 对。 胺反应形式,活化的 NHS 酯。

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  • Coumarin 343 X NHS ester

Coumarin 343 X NHS ester

标签:NHS esters Coumarin 343 Protein labeling
货号 规格 价格 货期
12620 1 mg 110.00$ 5 days
22620 5 mg 210.00$ in stock
42620 25 mg 410.00$ in stock
52620 50 mg 695.00$ in stock
62620 100 mg 1190.00$ in stock

Blue emitting Coumarin 343 dye can form a FRET pair with fluorescein (FAM). An amine reactive form, activated NHS ester.

This amine-reactive NHS ester contains an aminohexanoyl linker between the fluorophore, and the reactive group. This linker provides better solubility and spatial separation between the fluorophore, and the target molecule being labeled.

This reagent can be used to design FRET based assays with FAM as acceptor, and to construct systems which harvest blue light energy.

蓝色发射香豆素 343 染料可与荧光素 (FAM) 形成 FRET 对。 胺反应形式,活化的 NHS 酯。

这种胺反应性 NHS 酯在荧光团和反应性基团之间含有氨基己酰基接头。 这种接头在荧光团和被标记的目标分子之间提供了更好的溶解度和空间分离。

该试剂可用于设计以 FAM 为受体的基于 FRET 的分析,并构建收集蓝光能量的系统。

Coumarin 343 X NHS ester Coumarin 343 X NHS ester Coumarin 343 X NHS ester Coumarin 343 X NHS ester

Absorption and emission spectra of Coumarin 343

Coumarin 343 X NHS ester

Coumarin 343 X NHS ester相关产品

AF568 NHS ester

AF568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.

AF568 衍生物,用于用游离胺标记蛋白质和其他高分子量和低分子量配体。

Sulfo-Cyanine3 tetrazine

Water soluble sulfo-Cyanine3 dye tetrazine derivative for TCO ligation labeling with trans-cyclooctenes.

水溶性磺基-Cyanine3 染料四嗪衍生物,用于用反式环辛烯进行 TCO 连接标记。

BDP 581/591 carboxylic acid

BDP 581/591 is a multi purpose fluorophore, that can also be used to probe reactive oxygen species (ROS). This is a carboxylic acid derivative with a free COOH group.

BDP 581/591 是一种多用途荧光团,也可用于探测活性氧 (ROS)。 这是一种带有游离 COOH 基团的羧酸衍生物。

General properties

Appearance: yellow solid
Molecular weight: 495.52
CAS number: 946123-12-2
Molecular formula: C26H29N3O7
Solubility: good in DCM, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 437
ε, L⋅mol−1⋅cm−1: 39000
Emission maximum, nm: 477
Fluorescence quantum yield: 0.63
CF260: 0.29
CF280: 0.24

相关文献:

  1. Zheng, L.; Zhao, H.; Han, Y.; Qian, H.; Vukovic, L.; Mecinović, J.; Král, P.; Huck, W.T.S. Catalytic transport of molecular cargo using diffusive binding along a polymer track. Nature Chemistry, 2019, 11(4), 359–366. doi: 10.1038/s41557-018-0204-7