标签： 巨噬细胞炎症蛋白 5 人重组CHM-011

Small inducible cytokine A15 precursor, CCL15, Macrophage inflammatory protein 5, MIP-5, MIP5, Chemokine CC-2, HCC-2, NCC-3, MIP- 1 delta, Leukotactin-1, LKN-1, Mrp-2b, C-C motif chemokine 15.

CCL15, a new human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid protein containing a putative signal peptide of 21 amino acids that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1?, respectively. The gene for MIP-5 is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-5 is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

CCL15 是一种新的人类 CC 趋化因子，是从人类胎儿脾脏 cDNA 文库中分离出来的。 CCL15 cDNA 编码一个预测的 113 个氨基酸的蛋白质，该蛋白质含有一个推定的 21 个氨基酸的信号肽，该信号肽被切割以产生一个 92 个氨基酸残基的成熟蛋白质。 在 CC 家族成员中，人 CCL15 分别与小鼠 C10、人 MPIF-1、人 HCC-1 和小鼠 MIP-1? 具有 45%、44%、35% 和 30% 的 aa 同源性。 MIP-5 的基因位于 17 号染色体上，大多数人类 CC 趋化因子的基因都位于该处。 人 CCL15 在 T 和 B 淋巴细胞、NK 细胞、单核细胞和单核细胞衍生的树突细胞中表达。 人 MIP-5 对 T 细胞和单核细胞具有趋化性，并且已显示在人 CCR-1 转染的细胞中诱导钙通量。

Macrophage Inflammatory Protein-5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 68 amino acids and having a molecular mass of 7.4kDa. The MIP5 is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

MIP5 was lyophilized from a 0.2µm filtered concentrated solution containing PBS, pH-7.4.

It is recommended to reconstitute the lyophilized MIP5 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized MIP-5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL15 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 MIP-5 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CCL15 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

SFHFAADCCT SYISQSIPCS LMKSYFETSS ECSKPGVIFL TKKGRQVCAK PSGPGVQDCM KKLKPYSI.

Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is typically 2-4ng/ml.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.