标签： 巨噬细胞炎症蛋白 3 Beta 小鼠重组CHM-341

Small inducible cytokine A19, CCL19, Macrophage inflammatory protein 3 beta, MIP-3- beta, EBI1-ligand chemokine, ELC, Beta chemokine exodus-3, CK beta-11, chemokine ligand 19, CKb11, MIP3B, MIP-3b, SCYA19, MGC34433, Epstein-Barr virus-induced molecule 1 ligand chemokine, EBI1-ligand chemokine.

Chemokine ligand 19 is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine and macrophage inflammatory protein-3-beta . CCL19 is expressed abundantly in thymus and lymph nodes, with moderate levels in trachea and colon and low levels in stomach, small intestine, lung, kidney and spleen. The gene for CCL19 is located on human chromosome 9. This chemokine elicits its effects on its target cells by binding to the chemokine receptor chemokine receptor CCR7. It attracts certain cells of the immune system, including dendritic cells and antigen-engaged B cells.

趋化因子配体 19 是一种小细胞因子，属于 CC 趋化因子家族，也称为 EBI1 配体趋化因子和巨噬细胞炎症蛋白-3-β。 CCL19 在胸腺和淋巴结中大量表达，在气管和结肠中表达水平适中，在胃、小肠、肺、肾和脾脏中表达水平较低。 CCL19 的基因位于人类第 9 号染色体上。该趋化因子通过与趋化因子受体趋化因子受体 CCR7 结合而对其靶细胞产生影响。 它吸引免疫系统的某些细胞，包括树突状细胞和抗原结合的 B 细胞。

Macrophage Inflammatory Protein-3 beta Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9216 Dalton.
The MIP-3b is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The CCL19 was lyophilized from a concentrated solution in water containing no additives.

It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-3b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的巨噬细胞炎症蛋白-3b 用不低于 100µg/ml 的无菌 18MΩ-cm H2O 复溶，然后可以进一步稀释成其他水溶液。

Lyophilized MIP-3b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL19 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Ala-Asn-Asp-Ala.

The Activity is calculated by the ability to chemoattract Human mature dendritic cells using a concentration range of 8-80 ng/ml.

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a calibrated solution of MIP-3b as a Reference Standard.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.