标签： 巨噬细胞炎症蛋白 3 人重组CHM-358

C-C motif chemokine 23, Small-inducible cytokine A23, Macrophage inflammatory protein 3, Myeloid progenitor inhibitory factor 1, CK-beta-8, MIP-3, MPIF-1, CKB-8, CCL23, MIP3, MPIF1, SCYA23, CKb8, Ckb-8-1.

CCL23 is a ligand for the CCR1chemokine receptor. CCL23 is one of several cytokine genes clustered on the q-arm of chromosome 17, in a locus containing several other CC chemokines. MIP-3 chemoattracts monocytes, resting T-lymphocytes and neutrophils, but not activated lymphocytes. Furthermore, it was shown that MIP-3 inhibits colony formation of bone marrow myeloid immature progenitors. MIP-3 is mainly expressed in lung and liver tissue, but can be also found in bone marrow and placenta, as well as in some cell lines of myeloid origin.
Alternative splicing of the CCL23 gene produces 2 mRNAs which encode a short and a long isoform of the MIP-3. CK?8 cDNA encodes a 120 amino acid residue precursor protein with a putative 21 a.a. residue signal peptide which is cleaved to generate a 99 a.a. residue mature CK?8 . Further N-terminal processing of the 99 a.a. residue variant can produce a 75 a.a. residue CK?8 which is considerably more active than the 99 a.a. residue variant.
MIP-3 may be involved in the malignant progression of certain human cancer cells which overexpress ErbB2 through the transactivation of ErbB2 tyrosine kinase. MIP-3 may also be involved in angiogenesis via upregulation of matrix metalloproteinase MMP-2 expression.

CCL23 是 CCR1 趋化因子受体的配体。 CCL23 是聚集在 17 号染色体 q 臂上的几个细胞因子基因之一，位于包含其他几个 CC 趋化因子的基因座中。 MIP-3 趋化单核细胞、静息 T 淋巴细胞和中性粒细胞，但不吸引活化的淋巴细胞。此外，显示 MIP-3 抑制骨髓髓系未成熟祖细胞的集落形成。 MIP-3 主要在肺和肝组织中表达，但也可见于骨髓和胎盘，以及一些骨髓来源的细胞系。
CCL23 基因的可变剪接产生 2 个 mRNA，它们编码 MIP-3 的短同种型和长同种型。 CK?8 cDNA 编码一个 120 个氨基酸残基的前体蛋白，推定为 21 个氨基酸。残基信号肽被切割产生 99 a.a.残基成熟CK?8。 99 a.a. 的进一步 N 端处理残基变体可产生 75 a.a. CK?8 比 99 a.a. 活性更高。残基变体。
MIP-3 可能参与某些人类癌细胞的恶性进展，这些癌细胞通过 ErbB2 酪氨酸激酶的反式激活过度表达 ErbB2。 MIP-3 也可能通过上调基质金属蛋白酶 MMP-2 的表达而参与血管生成。

MIP-3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 99 amino acids and having a molecular mass of 11.3kDa.
The MIP-3 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 MIP-3 Human Recombinant 是一条单一的、非糖基化的多肽链，含有 99 个氨基酸，分子量为 11.3kDa。
MIP-3 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Filtered and lyophilized from a concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.

It is recommended to reconstitute the lyophilized MIP-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized MIP-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL23 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 MIP-3 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CCL23 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESYFETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN.

Determined by its ability to chemoattract human T cell population using a concentration range of 10-50ng/ml corresponding to a Specific Activity of 20,000-100,000IU/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.