标签： 巨噬细胞炎症蛋白-1 Alpha 人类重组CHM-233

Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA.

Macrophage Inflammatory Proteins belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now officially named CCL3 and CCL4 respectively. Both are major factors produced by macrophages after they are stimulated with bacterial endotoxins. They activate human granulocytes which can lead to acute neutrophilic inflammation. They also induce the synthesis and release of other pro-inflammatory cytokines such as interleukin 1 , IL-6 and TNF-a from fibroblasts and macrophages. The genes for CCL3 and CCL4 are both located on human chromosome 17.

巨噬细胞炎症蛋白属于称为趋化因子的趋化细胞因子家族。 在人类中，有两种主要形式，MIP-1a 和 MIP-1b，现在分别正式命名为 CCL3 和 CCL4。 两者都是巨噬细胞受到细菌内毒素刺激后产生的主要因子。 它们激活可导致急性中性粒细胞炎症的人类粒细胞。 它们还诱导成纤维细胞和巨噬细胞合成和释放其他促炎细胞因子，例如白细胞介素1、IL-6和TNF-α。 CCL3 和 CCL4 的基因都位于人类第 17 号染色体上。

Macrophage Inflammatory Protein-1 alpha Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7820 Dalton.
The MIP-1a is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的巨噬细胞炎症蛋白-1 α 人重组体是一种单一的、非糖基化的多肽链，包含 70 个氨基酸，分子量为 7820 道尔顿。
MIP-1a 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The protein was lyophilized from 0.55 mg/ml solution containing no additives.

It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized MIP-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Ser-Leu-Ala-Ala.

The Activity is calculated by the ability of chemo-attraction of Human monocytes using 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.