NEB代理 , 核酸纯化 , 质粒小提

产品资料 – 核酸纯化 – 质粒小提

Monarch 质粒小提试剂盒                             

货 号
规 格
价 格(元)

#T1010L
250 preps
4,379.00

#T1010S
50 preps
1,039.00

      


相关产品

Monarch 质粒小提离心柱
Monarch 质粒重悬缓冲液(B1)
Monarch 质粒裂解缓冲液(B2)
Monarch 质粒中和缓冲液(B3)
Monarch 质粒漂洗缓冲液 1
Monarch 质粒漂洗缓冲液 2
Monarch DNA 洗脱缓冲液
紫色凝胶上样染料 6X,无 SDS
Monarch DNA 胶回收试剂盒
Monarch PCR & DNA 纯化试剂盒 (5 μg)

特性

  更小的洗脱体积
  优化的离心柱设计消除了缓冲液和 盐的残留
  有色缓冲液系统来监控特定步骤的 完成
  实验开始前无需加入 RNA 酶
  为了方便使用,可购买经过优化的 试剂盒或单独购买缓冲液和离心柱
  标记区及磨砂表面更容易书写标记

概述

Monarch 质粒小提试剂盒提供了可以快速可靠的纯化高质量质粒 DNA 的方法。该方法除了采用了标准的细胞重悬、碱性裂解及中和等步骤外,还在特定步骤加入了颜色指示,以便于监控实验的完成。离心纯化菌裂解物后,DNA 在高盐条件下结合到专有的二氧化硅基质上。独特的漂洗缓冲液可以保证盐、蛋白、RNA 及其它细胞组分(内毒素)被去除干净,而较小的洗脱体积也保证了可以得到较高浓度和纯度的 DNA,可以直接用于限制性酶切、DNA 测序、PCR 及其它酶操作实验。
 
NEB代理 , 核酸纯化 , 质粒小提
Monarch 质粒小提试剂盒与市场一类厂家比较,产量、纯度和功能一致,但获得的质
粒DNA浓度最高。实验采用相同的 1.5 ml 过夜培养的菌液。根据各厂家提供的实验步
骤操作,抽提结束后各取 1 μl 用 HindIII-HF (NEB #R3104) 酶切,1% 的琼脂糖凝胶电泳。

 
NEB代理 , 核酸纯化 , 质粒小提
独特的 Monarch 小提离心柱设计

Monarch 质粒小提试剂盒组分:

– Monarch 质粒小提离心柱
– Monarch 质粒重悬缓冲液(B1)(粉色)
– Monarch 质粒裂解缓冲液(B2)(蓝色/绿色)
– Monarch 质粒中和缓冲液(B3)(黄色)
– Monarch 质粒漂洗缓冲液 1
– Monarch 质粒漂洗缓冲液 2(5X)
– Monarch DNA 洗脱缓冲液

为了您的使用方便,Monarch 试剂盒组分, 包括离心柱和缓冲液都可以单独购买。

NEB代理 , 核酸纯化 , 质粒小提

产品资料 – 核酸纯化 – 质粒小提

Monarch 质粒重悬缓冲液(B1)                             

货 号
规 格
价 格(元)

#T1011L
55 ml
499.00

      


Description

Monarch Plasmid Resuspension Buffer (B1) is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L). This buffer is the first one used in the miniprep workflow and is used to resuspend the cell pellet after the initial centrifugation of your cell culture. For easy identification, this buffer is colored pink.

Learn More About Monarch

  • NEBMonarch.com
  • Optimize your results with our unique column design
  • Enhance your DNA purification experience
  • Feel good about choosing Monarch
  • Choose Monarch kits for pure value

Properties and Usage

Storage Temperature

25°C 

Related Products

Companion Products

  • Monarch™ Plasmid Miniprep Kit 
  • Monarch™ Plasmid Miniprep Columns
  • Monarch™ DNA Elution Buffer
  • Monarch™ Plasmid Wash Buffer 2
  • Monarch™ Plasmid Wash Buffer 1
  • Monarch™ Plasmid Lysis Buffer (B2)
  • Monarch™ Plasmid Neutralization Buffer (B3)

NEB代理 , 核酸纯化 , 质粒小提

产品资料 – 核酸纯化 – 质粒小提

Monarch 质粒中和缓冲液(B3)                             

货 号
规 格
价 格(元)

#T1013L
110 ml
809.00

      


Description

Monarch Plasmid Neutralization Buffer is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L). Please note that this buffer contains RNase A and should be stored at 4°C after opening. This buffer is used to neutralize the lysate and digest any RNA present. The buffer also prepares the DNA for binding to the column matrix. It is conveniently colored yellow for identification as well as for monitoring when the neutralization is complete.

Learn More About Monarch

  • NEBMonarch.com
  • Optimize your results with our unique column design
  • Enhance your DNA purification experience
  • Feel good about choosing Monarch
  • Choose Monarch kits for pure value

Properties and Usage

Storage Temperature

4°C 

Related Products

Companion Products

  • Monarch™ Plasmid Miniprep Kit 
  • Monarch™ Plasmid Miniprep Columns
  • Monarch™ DNA Elution Buffer
  • Monarch™ Plasmid Wash Buffer 2
  • Monarch™ Plasmid Resuspension Buffer (B1)
  • Monarch™ Plasmid Lysis Buffer (B2)
  • Monarch™ Plasmid Wash Buffer 1

Notes

1.    Contains RNase A

 

NEB代理 , 核酸纯化 , 质粒小提

产品资料 – 核酸纯化 – 质粒小提

Monarch 质粒漂洗缓冲液 1                             

货 号
规 格
价 格(元)

#T1014L
54 ml (2 x 27ml)
499.00

      


Description

Monarch Plasmid Wash Buffer 1 is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L). This is the first wash buffer used to wash the DNA and remove cellular components after the initial binding to the column.

Learn More About Monarch

  • NEBMonarch.com
  • Optimize your results with our unique column design
  • Enhance your DNA purification experience
  • Feel good about choosing Monarch
  • Choose Monarch kits for pure value

Properties and Usage

Storage Temperature

25°C 

Related Products

Companion Products

  • Monarch™ Plasmid Miniprep Kit 
  • Monarch™ Plasmid Miniprep Columns
  • Monarch™ DNA Elution Buffer
  • Monarch™ Plasmid Wash Buffer 2
  • Monarch™ Plasmid Resuspension Buffer (B1)
  • Monarch™ Plasmid Lysis Buffer (B2)
  • Monarch™ Plasmid Neutralization Buffer (B3)

NEB代理 , 核酸纯化 , 质粒小提

产品资料 – 核酸纯化 – 质粒小提

Monarch 质粒漂洗缓冲液 2                             

货 号
规 格
价 格(元)

#T1015L
30 ml
499.00

      


Description

Monarch Plasmid Wash Buffer 2 is designed for use with the Monarch Plasmid Miniprep Kit (T1010S/L). This is the second wash buffer used to wash the DNA and remove trace buffer components, including salts.

Learn More About Monarch

  • NEBMonarch.com
  • Optimize your results with our unique column design
  • Enhance your DNA purification experience
  • Feel good about choosing Monarch
  • Choose Monarch kits for pure value

Properties and Usage

Storage Temperature

25°C 

Related Products

Companion Products

  • Monarch™ Plasmid Miniprep Kit 
  • Monarch™ Plasmid Miniprep Columns
  • Monarch™ DNA Elution Buffer
  • Monarch™ Plasmid Wash Buffer 1
  • Monarch™ Plasmid Resuspension Buffer (B1)
  • Monarch™ Plasmid Lysis Buffer (B2)
  • Monarch™ Plasmid Neutralization Buffer (B3)

NEB代理 , 核酸纯化 , 质粒小提

产品资料 – 核酸纯化 – 质粒小提

Monarch 质粒小提离心柱                             

货 号
规 格
价 格(元)

#T1017L
100 columns
1,109.00

      


Description

Monarch Plasmid Miniprep Columns, supplied with the Monarch Plasmid Miniprep Kit, have been custom designed to deliver excellent performance for your plasmid purification. Monarch Columns are designed without a frit (which is commonly used in purification columns to hold the membrane in place), eliminating buffer retention and the risk of carry-over contamination. The tapered design of the Miniprep Column enables elution in as little as 30 μl. Monarch columns use less plastic than conventional columns, reducing their environmental footprint without compromising performance. The columns fit snugly into the collection tubes to enable easy handling. The Monarch Miniprep Columns also feature a convenient tab so that you can label your columns easily without removing them from your tube rack. Monarch columns are suitable for use with centrifugation and vacuum purification protocols. 

Monarch Plasmid Miniprep Column Design

NEB代理 , 核酸纯化 , 质粒小提

Learn More About Monarch

  • NEBMonarch.com
  • Optimize your results with our unique column design
  • Enhance your DNA purification experience
  • Feel good about choosing Monarch
  • Choose Monarch kits for pure value

Highlights

  • No buffer retention/risk of carryover contamination
  • Low elution volume (≥ 30 μl)
  • Convenient tab for easy labeling
  • Snug fit into collection tubes for easy handling
  • Made with less plastic than conventional columns

 

Properties and Usage

Storage Temperature

25°C

Binding Capacity

20 µg