标签： 单核细胞趋化蛋白-1小鼠重组CHM-313

Small inducible cytokine A2, CCL2, Monocyte chemotactic protein 1, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte secretory protein JE, HC11, chemokine ligand 2, MCP1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434, GDCF-2 HC11, Platelet-derived growth factor-inducible protein JE.

Chemokine ligand 2 is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 . It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor ?B . CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acidsand a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weight of approximately 13kDa. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5.

趋化因子配体 2 是属于 CC 趋化因子家族的小细胞因子，也称为单核细胞趋化蛋白 1。 它存在于牙齿萌出和骨骼退化的部位。 在骨骼中，CCL2 由成熟的破骨细胞和成骨细胞表达，并受核因子 βB 的控制。 CCL2 将免疫细胞（例如单核细胞）募集到组织损伤和感染部位。 该趋化因子作为蛋白质前体产生，含有23个氨基酸的信号肽和76个氨基酸的成熟肽。 它是一种单体多肽，分子量约为 13kDa。 与许多其他 CC 趋化因子一样，CCL2 位于人类的第 17 号染色体上。 结合 CCL2 的细胞表面受体是 CCR2 和 CCR5。

Monocyte Chemotactic Protein-1 Mouse Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14 kDa.
The MCP-1 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的单核细胞趋化蛋白 1 小鼠重组体是一种单一的、非糖基化的多肽链，含有 125 个氨基酸，分子量为 14 kDa。
MCP-1 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The protein was lyophilized with no additives.

It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized MCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 MCP-1 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CCL2 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 90.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Val.

The biological activity was determined by calculating its ability to chemoattract Balb/C mouse spleen MNCs at 1.0-20.0 ng/ml.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.