ProSpec-TGFB2 Human, HEK

Description

Catalogue number

CYT-112

Synonyms

Transforming growth factor, beta 2, cetermin, Glioblastoma-derived T-cell suppressor factor, polyergin, G-TSF, TGF-beta2, TGF-beta-2, transforming growth factor beta-2, BSC-1 cell growth inhibitor, TGFB-2.

Introduction

TGFB2 is a 27.08 kDa protein having two identical 118 amino acid peptide chains linked by a single disulfide bond. TGFB2 is part of a family of five related cytokines that have an extensive variation of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-beta signal through the same receptor and stimulate similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing.

Description

TGF-b 2 Human Recombinant produced in HEK cells is a non-glycosylated homodimer, having a total molecular weight of 25kDa.
The TGF-b 2 is purified by proprietary chromatographic techniques.

Source

HEK.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

TGF-b 2 was lyophilized from a 0.2µm filtered solution containing 50mM sodium acetate pH 4.5.

Solubility

It is recommended to reconstitute the lyophilized TGF-b 2 in sterile solution containing 20% ethanol, 50mM sodium acetate and 75mM acetic acid.

Stability

Lyophilized TGF-b 2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGF-b 2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 95% as obsereved by -PAGE.

Biological Activity

The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells , the ED₅₀ is 0.16ng/ml.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TRAIL Mouse/CYT-806

ProSpec-TRAIL Mouse,ProSpec CYT-806,在大肠杆菌中产生的 TRAIL 重组小鼠是一条单一的、非糖基化的多肽链，含有 175 个氨基酸，分子量为 20.2kDa。

TRAIL Mouse

TNF-Related Apoptosis Inducing Ligand/Apo2L Mouse Recombinant
CYT-806
ProSpec-TRAIL Mouse/CYT-806 10µg
ProSpec-TRAIL Mouse/CYT-806 50µg
ProSpec-TRAIL Mouse/CYT-806 1mg

Catalogue number

CYT-806

Synonyms

Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, Apo-2 ligand, Apo-2L, CD253 antigen, TL2, APO2L, TNFSF10.

Introduction

TNF-related apoptosis-inducing ligand is a ligand molecule which induces apoptosis. It is a type II transmembrane protein with homology to other members of the tumor necrosis factor family.

In humans, the gene that encodes for TRAIL is located at chromosome 3q26. TRAIL binds to the death receptors, DR4 and DR5. The process of apoptosis is caspase-8-dependent. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues.

TNF相关的凋亡诱导配体是诱导细胞凋亡的配体分子。它是一种 II 型跨膜蛋白，与肿瘤坏死因子家族的其他成员具有同源性。
在人类中，编码 TRAIL 的基因位于染色体 3q26。 TRAIL 与死亡受体 DR4 和 DR5 结合。细胞凋亡过程是 caspase-8 依赖性的。这种蛋白质优先诱导转化细胞和肿瘤细胞凋亡，但似乎不会杀死正常细胞，尽管它在大多数正常组织中以显着水平表达。

Description

TRAIL Recombinant Mouse produced in E.coli is a single, non-glycosylated polypeptide chain containing 175 amino acids and having a molecular mass of 20.2kDa.

在大肠杆菌中产生的 TRAIL 重组小鼠是一条单一的、非糖基化的多肽链，含有 175 个氨基酸，分子量为 20.2kDa。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized containing PBS, pH 7.4, and 3mM DTT.

Solubility

It is recommended to reconstitute the lyophilized TRAIL Mouse Recombinant in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100 µg/ml 的无菌 18MΩ-cm H2O 中重新配制冻干的 TRAIL 小鼠重组体，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TRAIL although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TRAIL recombinant should be stored at 4C between 2-7 days and for future use below -18C.

For long term storage it is recommended to add a carrier protein .

Please prevent freeze-thaw cycles.

冻干的 TRAIL 虽然在室温下可稳定保存 3 周，但应在 -18C 以下干燥储存。重组后，TRAIL 重组体应在 4C 下储存 2-7 天，以备将来在 -18C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:

Analysis by RP-HPLC.

Analysis by -PAGE.

Amino acid sequence

MPRGGRPQKV AAHITGITRR SNSALIPISK DGKTLGQKIE SWESSRKGHS FLNHVLFRNG ELVIEQEGLY YIYSQTYFRF QEAEDASKMV SKDKVRTKQL VQYIYKYTSY PDPIVLMKSA RNSCWSRDAE YGLYSIYQGG LFELKKNDRI FVSVTNEHLM DLDQEASFFG AFLIN

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2,000,000 IU/mg in the presence of actinomycin D.

与标准相比具有完全的生物活性。使用鼠 L929 细胞通过细胞毒性试验确定的 ED50 小于 0.5 ng/ml，对应于在放线菌素 D 存在下 > 2,000,000 IU/mg 的比活性。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TGFB3 (207 a.a.) Human

Description

Catalogue number

CYT-319

Synonyms

Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.

Introduction

Transforming growth factor betas mediate many cell-cell interactions that occur during embryonic development. Three TGF Betas have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.

Description

TGFB3 Human Recombinant protein encoding amino acids 644-850 and total molecular mass of 50 kDa .

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

100µl purified human TGF Beta 3protein at 100µg/ml in 50mM Tris-Acetate, pH-7.5, 1mM EDTA and 20% Glycerol.

Stability

TGF-beta 3 Human Recombinant although stable at 4°C for 1 week, should be stored at -20°C. For long term storage it is recommended to add a carrier protein .
Please avoid freeze-thaw cycles.

Applications

ELISA.
Western Blotting.
Inhibition Assays.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-SCGB1A1 Human/CYT-743

ProSpec-SCGB1A1 Human,ProSpec CYT-743,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,子宫珠蛋白属于分泌珠蛋白超家族，是一种多功能蛋白，通过结合磷脂和前列腺素等疏水小分子发挥抗炎和抗肿瘤作用。子宫珠蛋白参与多种功能，包括抗炎、抑制磷脂酶 A2 和螯合疏水配体。

SCGB1A1 Human

Uteroglobin Human Recombinant
CYT-743
ProSpec-SCGB1A1 Human/CYT-743 10µg
ProSpec-SCGB1A1 Human/CYT-743 50µg
ProSpec-SCGB1A1 Human/CYT-743 1mg

Catalogue number

CYT-743

Synonyms

Uteroglobin, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, Secretoglobin family 1A member 1, Urinary protein 1, UP-1, UP1, Urine protein 1, SCGB1A1, CCSP, UGB, CC16.

Introduction

Uteroglobin which belongs to the Secretoglobin superfamily, is a multifunctional protein that exerts anti-inflammatory and anti-tumorigenic effects by binding small hydrophobic molecules such as phospholipids and prostaglandins. Uteroglobin is involved in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. SCGB1A1 is expressed by Clara cells, the non-ciliated, non-mucous secretory cells predominant in lung bronchioles, and by other epithelia which communicate with the external environment. On top of sequestering pro-inflammatory mediators and carcinogens, Uteroglobin is implicated in the inhibition of cell migration and invasion, platelet aggregation, and T cell differentiation. SCGB1A1 gene defects are associated with a susceptibility to asthma.

子宫珠蛋白属于分泌珠蛋白超家族，是一种多功能蛋白，通过结合磷脂和前列腺素等疏水小分子发挥抗炎和抗肿瘤作用。子宫珠蛋白参与多种功能，包括抗炎、抑制磷脂酶 A2 和螯合疏水配体。 SCGB1A1 由 Clara 细胞（主要存在于肺细支气管中的非纤毛、非粘液分泌细胞）和与外部环境通信的其他上皮细胞表达。除了隔离促炎介质和致癌物外，子宫珠蛋白还参与抑制细胞迁移和侵袭、血小板聚集和 T 细胞分化。 SCGB1A1 基因缺陷与哮喘易感性有关。

Description

Uteroglobin Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 15.8kDa.
The SCGB1A1 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The Uteroglobin protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized SCGB1A1 in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Uteroglobin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCGB1A1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干子宫珠蛋白虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。重组后，SCGB1A1 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

EICPSFQRVI ETLLMDTPSS YEAAMELFSP DQDMREAGAQ LKKLVDTLPQ KPRESIIKLM EKIAQSSLCN.

Biological Activity

The ED₅₀ as determined by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0µg/ml, corresponding to a specific activity of > 200 IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TGFB3 (24-412 a.a.) Human

Description

Catalogue number

CYT-886

Synonyms

Transforming Growth Factor, Beta 3, Prepro-Transforming Growth Factor Beta-3, TGF-Beta-3, ARVD1, RNHF, Arrhythmogenic Right Ventricular Dysplasia 1, Transforming Growth Factor Beta-3, TGF-Beta3, ARVD, Transforming growth factor beta-3, TGF-beta-3.

Introduction

Description

TGFB3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 412 amino acids and having a molecular mass of 47.2kDa.

TGFB3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TGFB3 protein solution containing 20mM Tris-HCl and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSLSTCTTL DFGHIKKKRV EAIRGQILSK LRLTSPPEPT VMTHVPYQVL ALYNSTRELL EEMHGEREEG CTQENTESEY YAKEIHKFDM IQGLAEHNEL AVCPKGITSK VFRFNVSSVE KNRTNLFRAE FRVLRVPNPS SKRNEQRIEL FQILRPDEHI AKQRYIGGKN LPTRGTAEWL SFDVTDTVRE WLLRRESNLG LEISIHCPCH TFQPNGDILE NIHEVMEIKF KGVDNEDDHG RGDLGRLKKQ KDHHNPHLIL MMIPPHRLDN PGQGGQRKKR ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-SCGB1A1 Human, His/CYT-752

ProSpec-SCGB1A1 Human His,ProSpec CYT-752,建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 SCGB1A1 不是无菌的！在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

SCGB1A1 Human, His

Uteroglobin Human Recombinant, His Tag
CYT-752
ProSpec-SCGB1A1 Human, His/CYT-752 2µg
ProSpec-SCGB1A1 Human, His/CYT-752 10µg
ProSpec-SCGB1A1 Human, His/CYT-752 1mg

Catalogue number

CYT-752

Synonyms

Introduction

Description

Uteroglobin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and including a 10 a.a N-terminal His tag. The total molecular mass is 9.2kDa .

在大肠杆菌中产生的 Uteroglobin Human Recombinant 是一种单一的、非糖基化的多肽链，含有 80 个氨基酸并包括一个 10 个氨基酸的 N 端 His 标签。总分子量为9.2kDa。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. SCGB1A1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 SCGB1A1 不是无菌的！在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

将冻干蛋白储存在 -20°C。重组后分装产品以避免重复的冷冻/解冻循环。重组蛋白可在 4°C 下保存一段时间；在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

MKHHHHHHASEICPSFQRVI ETLLMDTPSS YEAAMELFSP DQDMREAGAQ LKKLVDTLPQ KPRESIIKLM EKIAQSSLCN.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TGFB3 Human

Description

Catalogue number

CYT-368

Synonyms

Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.

Introduction

Description

TGF-β 3 Human Recombinant produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing two 113 amino acid chains and having a total molecular mass of 25.8kDa.
The TGF-β 3 is purified by standard chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The protein solution contains 20% Ethanol and 10mM Acetic acid .

Stability

TGF-beta 3 although stable at room temperature for 1 week, should be stored at 4°C.

For long term storage it is recommended to add a carrier protein .

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS.

Biological Activity

The activity is determined by the ability to induce chondrogenic differentiation.

Protein content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a calibrated solution of TGF-b 3 as a Reference Standard.

References

1.Title: Altering the Architecture of Tissue Engineered Hypertrophic Cartilaginous Grafts Facilitates Vascularisation and Accelerates Mineralisation
Publication: DOI: 10.1371/journal.pone.0090716, March 04, 2014
Link: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0090716

2. Title: Oxygen tension differentially regulates the functional properties of cartilaginous tissues engineered from infrapatellar fat pad derived MSCs and articular chondrocytes
Publication: Osteoarthritis and Cartilage 18.10 : 1345-1354.
Link: http://www.sciencedirect.com/science/article/pii/S1063458410002293

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-SCGB1A1 Mouse/CYT-746

ProSpec-SCGB1A1 Mouse,ProSpec CYT-746,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP.

SCGB1A1 Mouse

Uteroglobin Mouse Recombinant
CYT-746
ProSpec-SCGB1A1 Mouse/CYT-746 10µg
ProSpec-SCGB1A1 Mouse/CYT-746 50µg
ProSpec-SCGB1A1 Mouse/CYT-746 1mg

Catalogue number

CYT-746

Synonyms

Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP.

Introduction

Description

Uteroglobin Mouse Recombinant produced in E.coli is a non-glycosylated disulfide-linked homodimeric protein containing 2×75 amino acids chains and having a molecular mass of 16.7kDa.
The SCGB1A1 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Uteroglobin Mouse Recombinant 是一种非糖基化的二硫键连接的同型二聚体蛋白，含有 2×75 个氨基酸链，分子量为 16.7kDa。
SCGB1A1 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The Uteroglobin protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized SCGB1A1 in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

DICPGFLQVL EALLMESESG YVASLKPFNP GSDLQNAGTQ LKRLVDTLPQ ETRINIMKLT EKILTSPLCK QDLRF.

Biological Activity

由固定化蛋白质支持 A549 人肺癌细胞粘附的能力确定的 ED50 小于 5.0μg/ml，对应于 > 200 IU/mg 的比活性。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TGFB3 Human, HEK

Description

Catalogue number

CYT-113

Synonyms

Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.

Introduction

Description

TGF-b 3 Human Recombinant produced in HEK cells is a non-glycosylated homodimer, having a total molecular weight of 25kDa.
The TGF-b 3 is purified by proprietary chromatographic techniques.

Source

HEK.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The TGF-b 3 was lyophilized from 1mg/ml in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized TGF-b 3 in sterile 4mM HCl containing 0.1% endotoxin-free recombinant HSA.

Stability

Lyophilized TGF-b 3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGF-b 3 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 95% as obsereved by -PAGE.

Biological Activity

The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells and is typically 0.1-0.5ng/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-SCGB1A1 Rat/CYT-820

ProSpec-SCGB1A1 Rat,ProSpec CYT-820,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP.

SCGB1A1 Rat

Uteroglobin Rat Recombinant
CYT-820
ProSpec-SCGB1A1 Rat/CYT-820 10µg
ProSpec-SCGB1A1 Rat/CYT-820 50µg
ProSpec-SCGB1A1 Rat/CYT-820 1mg

Catalogue number

CYT-820

Synonyms

Introduction

Description

SCGB1A1 Rat Recombinant produced in E.Coli is a homodimeric non-glycosylated polypeptide chains consisting of two 77 amino acids and having a molecular mass of 17.0kDa.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

SCGB1A1 protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized SCGB1A1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 SCGB1A1，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized SCGB1A1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCGB1A1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .

Please prevent freeze-thaw cycles.

冻干的 SCGB1A1 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。重组后，SCGB1A1 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

SSDICPGFLQ VLEALLLGSE SNYEAALKPF NPASDLQNAG TQLKRLVDTL PQETRINIVK LTEKILTSPL CEQDLRV

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0 ?g/ml, corresponding to a specific activity of >200IU/mg.

与标准相比具有完全的生物活性。由固定化蛋白质支持 A549 人肺癌细胞粘附的能力所确定的 ED50 小于 5.0 μg/ml，对应于 >200IU/mg 的比活性。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TGFB3 Human, Plant

Description

Catalogue number

CYT-588

Synonyms

Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.

Introduction

Description

TGFB3 Human Recombinant produced in plant is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 118 amino acids and having a molecular mass of 27.2kDa.
The TGFB3 is fused to 6xHis tag at N-terminus and purified by standard chromatographic techniques.

Source

Nicotiana benthamiana.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a concentrated solution containing 50mM Tris-HCl pH-7.4.

Solubility

It is recommended to reconstitute the lyophilized TGFB3 in sterile 5mM HCl & 50ug/ml BSA at a concentration of 0.05mg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TGFB3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB3 Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein . Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

HHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKG
YYANFCSGPCPYLRSADTTHSTVLGLY
NTLNPEASASP
CCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS.

Biological Activity

The biological activity of TGFB3 is measured in culture by its ability to inhibit the mink lung epithelial cells proliferation. ED50 ? 40ng/ml corresponding to a specific activity of 25,000 Units/mg.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-Visfatin Human/CYT-318

ProSpec-Visfatin Human,ProSpec CYT-318,The activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from human PBMCs at 100ng/ml.

Visfatin Human

Visfatin Human Recombinant
CYT-318
ProSpec-Visfatin Human/CYT-318 5µg
ProSpec-Visfatin Human/CYT-318 25µg
ProSpec-Visfatin Human/CYT-318 1mg

Catalogue number

CYT-318

Synonyms

PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.

Introduction

Excess adiposity is the most important risk in the development of type 2 diabetes mellitus . Adipose tissue produces several proteins such as leptin, adiponectin, resistin, tumor necrosis factor-a, and IL-6, that modulate sensitivity and appear to play an important role in the pathogenesis, diabetes, dyslipidemia, inflammation, and atherosclerosis. Visfatin, also known as pre-B cell colony-enhancing factor , is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL-7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, including humans and the mouse. It has been postulated to play a role in innate immunity.
Visfatin exerts mimetic effects that are dose-dependent and quantitatively similar to stimulating muscle and adipocyte glucose transport, and in inhibiting hepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose-dependent fashion. In keeping with its mimetic effects, visfatin was as effective in reducing hyperglycemia in deficient diabetic mice. Visfatin was also found to be bound to and activate receptor, causing receptor phosphorylation and the activation of downstream signaling molecules. However, visfatin did not compete for binding to the receptor, indicating that the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.

过度肥胖是 2 型糖尿病发展中最重要的风险。脂肪组织产生几种蛋白质，如瘦素、脂联素、抵抗素、肿瘤坏死因子-a 和 IL-6，它们调节敏感性并似乎在发病机制、糖尿病、血脂异常、炎症和动脉粥样硬化中起重要作用。 Visfatin，又称pre-B细胞集落增强因子，是一种在内脏脂肪中高度表达的细胞因子，最初作为一种分泌因子被分离出来，与IL-7和干细胞因子协同促进B细胞前体的生长.已在鲤鱼、无脊椎动物软体动物和细菌以及脊椎动物（包括人类和小鼠）中鉴定出 Visfatin 同源物。它被假定在先天免疫中发挥作用。
Visfatin 发挥剂量依赖性和数量上类似于刺激肌肉和脂肪细胞葡萄糖转运以及抑制肝细胞葡萄糖产生的模拟效应。在小鼠中静脉注射重组内脂素以剂量依赖性方式降低血浆葡萄糖。与其模拟效果一致，visfatin 在降低缺陷型糖尿病小鼠的高血糖方面同样有效。还发现 Visfatin 与受体结合并激活受体，导致受体磷酸化和下游信号分子的激活。然而，内脂素没有竞争与受体结合，表明这两种蛋白质被受体的不同区域识别。因此，内脂素可能在葡萄糖稳态和生物合成或信号转导中的失调中发挥作用，并可能有助于糖尿病的发病机制。

Description

Visfatin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 466 amino acids. The total molecular mass is 52.6kDa . The Visfatin is purified by Flag-affinity chromatography.

在大肠杆菌中产生的 Visfatin Human Recombinant 是单一的、非糖基化的多肽链，含有 466 个氨基酸。总分子量为52.6kDa。 Visfatin 通过Flag 亲和层析纯化。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Visfatin was lyophilized with no additives.

Solubility

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 20 mM HCl at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Wait several minutes for full reconstitution and solubility.

打开前离心小瓶。重新配制产品时，轻轻吸管并冲洗小瓶侧面，以确保蛋白质完全恢复到溶液中。建议用浓度为 0.1 mg/mL 的 20 mM HCl 复溶冻干产品，可进一步稀释成其他水溶液。等待几分钟完全重组和溶解。

Stability

Lyophilized Visfatin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Visfatin should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 Visfatin 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。复溶后，Visfatin 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH.

Biological Activity

The activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from human PBMCs at 100ng/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TGFB3 Mouse

Description

Catalogue number

CYT-143

Synonyms

Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.

Introduction

Description

TGF-β 3 Mouse Recombinant produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing two 113 amino acid chains and having a total molecular mass of 25.7kDa.

The TGF-β 3 is purified by standard chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The Mouse TGFB3 protein solution contains 20% Ethanol and 10mM Acetic acid.

Stability

Mouse TGF-beta 3 although stable at room temperature for 3 weeks, should be stored at 4°C.
For long term storage it is recommended to add a carrier protein .

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS.

Biological Activity

The activity is determined by the ability to induce chondrogenic differentiation.

Protein content

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-Visfatin Human, His/CYT-563

ProSpec-Visfatin Human His,ProSpec CYT-563,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,过度肥胖是发生胰岛素抵抗和 2 型糖尿病的最重要风险。脂肪组织产生几种蛋白质，如瘦素、脂联素、抵抗素、肿瘤坏死因子-α和 IL-6，它们调节胰岛素敏感性，似乎在胰岛素抵抗、糖尿病、血脂异常、炎症和动脉粥样硬化的发病机制中起重要作用.然而，脂肪组织诱导胰岛素抵抗的机制以及脂肪细胞因子在 T2DM 发病机制中的作用尚未完全确定。

Visfatin Human, His

Visfatin Recombinant Human, His Tag
CYT-563
ProSpec-Visfatin Human, His/CYT-563 5µg
ProSpec-Visfatin Human, His/CYT-563 25µg
ProSpec-Visfatin Human, His/CYT-563 1mg

Catalogue number

CYT-563

Synonyms

PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.

Introduction

Excess adiposity is the most important risk in the development of insulin resistance and type 2 diabetes mellitus . Adipose tissue produces several proteins such as leptin, adiponectin, resistin, tumor necrosis factor-?, and IL-6, that modulate insulin sensitivity and appear to play an important role in the pathogenesis of insulin resistance, diabetes, dyslipidemia, inflammation, and atherosclerosis. However, the mechanisms by which fat tissue induces insulin resistance and the role of adipocytokines in the pathogenesis of T2DM have not been well established.
Visfatin, also known as pre-B cell colony-enhancing factor , is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL-7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, including humans and the mouse. It has been postulated to play a role in innate immunity.
Visfatin exerts insulin-mimetic effects that are dose-dependent and quantitatively similar to those of insulin in stimulating muscle and adipocyte glucose transport, and in inhibiting hepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose-dependent fashion. In keeping with its insulin-mimetic effects, visfatin was as effective as insulin in reducing hyperglycemia in insulin-deficient diabetic mice. Visfatin was also found to be bound to and activate insulin receptor, causing receptor phosphorylation and the activation of downstream signaling molecules. However, visfatin and insulin did not compete for binding to the insulin receptor, indicating that the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.

过度肥胖是发生胰岛素抵抗和 2 型糖尿病的最重要风险。脂肪组织产生几种蛋白质，如瘦素、脂联素、抵抗素、肿瘤坏死因子-α和 IL-6，它们调节胰岛素敏感性，似乎在胰岛素抵抗、糖尿病、血脂异常、炎症和动脉粥样硬化的发病机制中起重要作用.然而，脂肪组织诱导胰岛素抵抗的机制以及脂肪细胞因子在 T2DM 发病机制中的作用尚未完全确定。
Visfatin，又称pre-B细胞集落增强因子，是一种在内脏脂肪中高度表达的细胞因子，最初作为一种分泌因子被分离出来，与IL-7和干细胞因子协同促进B细胞前体的生长.已在鲤鱼、无脊椎动物软体动物和细菌以及脊椎动物（包括人类和小鼠）中鉴定出 Visfatin 同源物。它被假定在先天免疫中发挥作用。
Visfatin 在刺激肌肉和脂肪细胞葡萄糖转运以及抑制肝细胞葡萄糖产生方面发挥剂量依赖性和数量上与胰岛素相似的胰岛素模拟作用。在小鼠中静脉注射重组内脂素以剂量依赖性方式降低血浆葡萄糖。与其类似胰岛素的作用相一致，visfatin 在降低胰岛素缺陷型糖尿病小鼠的高血糖方面与胰岛素一样有效。还发现 Visfatin 与胰岛素受体结合并激活胰岛素受体，导致受体磷酸化和下游信号分子的激活。然而，内脂素和胰岛素没有竞争与胰岛素受体的结合，表明这两种蛋白质被受体的不同区域识别。因此，内脂素可能在葡萄糖稳态和生物合成或信号转导中的失调中发挥作用，并可能有助于糖尿病的发病机制。

Description

Visfatin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 511 amino acids and having a molecular mass of 57 kDa.
The recombinant human Visfatin is fused to His tag at N-Terminus.

在大肠杆菌中产生的 Visfatin Human Recombinant 是一种单一的、非糖基化的多肽链，含有 511 个氨基酸，分子量为 57 kDa。
重组人 Visfatin 在 N 末端与 His 标签融合。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered solution at a concentration of 1mg/ml.

Formulation

Visfatin His tag protein contains 20mM Tris pH-8, 0.1mM DTT & 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
请避免冻融循环。

Purity

Greater than 95.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MNPAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKLR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKDVYKEH FQDDVFNEKG WNYILEKYDG HLPIEIKAVP EGFVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGLALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVV YDIYNACEKI WGEDLRHLIV SRSTQAPLII RPDSGNPLDT VLKVLEILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KMWSIENIAF GSGGGLLQKL TRDLLNCSFK CSYVVTNGLG INVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGQDLLHT VFKNGKVTKS YSFDEIRKNA QLNIELEAAH H.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-EG VEGF Human

Description

Catalogue number

CYT-338

Synonyms

PK1, PRK1, Prokineticin 1, EG-VEGF.

Introduction

Endocrine gland-derived vascular endothelial growth factor induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. Its expression is induced by hypoxia and is restricted to the steroidogenic glands . Its expression is often complementary to the expression of VEGF , suggesting that these molecules function in a coordinated manner. EG-VEGF potently contracts gastrointestinal smooth muscle. Induces proliferation, migration and fenestration in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types.

Description

EG-VEGF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids and having a molecular mass of 9.7kDa. The EG-VEGF is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a concentrated solution containing 0.1% Trifluoroacetic Acid .

Solubility

It is recommended to reconstitute the lyophilized Endocrine Gland Vascular Endothelial Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized EG-VEGF Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EG-VEGF should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Biological Activity

The activity as determined by the dose-dependent proliferation of MIA PaCa-2 cells is typically 1-4 μg/ml.

Amino acid sequence

AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-Visfatin Mouse/CYT-447

ProSpec-Visfatin Mouse,ProSpec CYT-447,如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。

Visfatin Mouse

Visfatin Mouse Recombinant
CYT-447
ProSpec-Visfatin Mouse/CYT-447 5µg
ProSpec-Visfatin Mouse/CYT-447 25µg
ProSpec-Visfatin Mouse/CYT-447 1mg

Catalogue number

CYT-447

Synonyms

PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.

Introduction

Excess adiposity is the most important risk in the development of insulin resistance and type 2 diabetes mellitus . Adipose tissue produces several proteins such as leptin, adiponectin, resistin, tumor necrosis factor-?, and IL-6, that modulate insulin sensitivity and appear to play an important role in the pathogenesis of insulin resistance, diabetes, dyslipidemia, inflammation, and atherosclerosis. However, the mechanisms by which fat tissue induces insulin resistance and the role of adipocytokines in the pathogenesis of T2DM have not been well established. Visfatin, also known as pre-B cell colony-enhancing factor , is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL-7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, including humans and the mouse. It has been postulated to play a role in innate immunity.
Visfatin exerts insulin-mimetic effects that are dose-dependent and quantitatively similar to those of insulin in stimulating muscle and adipocyte glucose transport, and in inhibiting hepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose-dependent fashion. In keeping with its insulin-mimetic effects, visfatin was as effective as insulin in reducing hyperglycemia in insulin-deficient diabetic mice. Visfatin was also found to be bound to and activate insulin receptor, causing receptor phosphorylation and the activation of downstream signaling molecules. However, visfatin and insulin did not compete for binding to the insulin receptor, indicating that the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.

过度肥胖是发生胰岛素抵抗和 2 型糖尿病的最重要风险。脂肪组织产生几种蛋白质，如瘦素、脂联素、抵抗素、肿瘤坏死因子-α和 IL-6，它们调节胰岛素敏感性，似乎在胰岛素抵抗、糖尿病、血脂异常、炎症和动脉粥样硬化的发病机制中起重要作用.然而，脂肪组织诱导胰岛素抵抗的机制以及脂肪细胞因子在 T2DM 发病机制中的作用尚未完全确定。 Visfatin，又称pre-B细胞集落增强因子，是一种在内脏脂肪中高度表达的细胞因子，最初作为一种分泌因子被分离出来，与IL-7和干细胞因子协同促进B细胞前体的生长.已在鲤鱼、无脊椎动物软体动物和细菌以及脊椎动物（包括人类和小鼠）中鉴定出 Visfatin 同源物。它被假定在先天免疫中发挥作用。
Visfatin 在刺激肌肉和脂肪细胞葡萄糖转运以及抑制肝细胞葡萄糖产生方面发挥剂量依赖性和数量上与胰岛素相似的胰岛素模拟作用。在小鼠中静脉注射重组内脂素以剂量依赖性方式降低血浆葡萄糖。与其类似胰岛素的作用相一致，visfatin 在降低胰岛素缺陷型糖尿病小鼠的高血糖方面与胰岛素一样有效。还发现 Visfatin 与胰岛素受体结合并激活胰岛素受体，导致受体磷酸化和下游信号分子的激活。然而，内脂素和胰岛素没有竞争与胰岛素受体的结合，表明这两种蛋白质被受体的不同区域识别。因此，内脂素可能在葡萄糖稳态和生物合成或信号转导中的失调中发挥作用，并可能有助于糖尿病的发病机制。

Description

Visfatin Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing a 20 aa His tag and having 511 amino acids. The total molecular mass is 57kDa. The Visfatin is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Visfatin Mouse Recombinant 是一条单一的、非糖基化的多肽链，含有 20 个氨基酸的 His 标签并具有 511 个氨基酸。总分子量为 57kDa。 Visfatin 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless 1mg/ml solution.

Formulation

The protein solution contains 1x PBS pH-7.4.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Please avoid freeze thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
请避免冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECREKKTENSKVR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKEVYREH FQDDVFNERGWNYILEKYDG HLPIEVKAVP EGSVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPITVATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVV YDIYNACEKI WGEDLRHLIV SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KKWSIENVSF GSGGALLQKL TRDLLNCSFK CSYVVTNGLG VNVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGHDLLHTVFKNGKVTKS YSFDEVRKNA QLNIEQDVAP H.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-EG VEGF Mouse

Description

Catalogue number

CYT-825

Synonyms

PK1, Prokineticin 1, EG-VEGF, Prok1, Endocrine-gland-derived vascular endothelial growth factor.

Introduction

Description

EG-VEGF Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids and having a molecular mass of 9.6kDa.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS pH7.4 and 3% Trehalose.

Solubility

Stability

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

AVITGACERD IQCGAGTCCA ISLWLRGLRL CTPLGREGEE CHPGSHKIPF LRKRQHHTCP CSPSLLCSRF PDGRYRCFRD LKNANF.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-WISP1 Human/CYT-1084

ProSpec-WISP1 Human,ProSpec CYT-1084,WNT1 Inducible Signaling Pathway Protein 1, CCN Family Member 4, CCN4, Wnt-1 Inducible, Signaling Pathway Protein 1, WNT1 Induced Secreted Protein 1, Wnt-1-Induced Secreted Protein, CTC-458A3.8, WISP1tc, WISP1c, WISP1i, WISP-1, WISP1.

WISP1 Human
WNT1 Inducible Signaling Pathway Protein 1 Human Recombinant
CYT-1084
2µg
10µg
1mg

Catalogue number

CYT-1084

Synonyms

WNT1 Inducible Signaling Pathway Protein 1, CCN Family Member 4, CCN4, Wnt-1 Inducible, Signaling Pathway Protein 1, WNT1 Induced Secreted Protein 1, Wnt-1-Induced Secreted Protein, CTC-458A3.8, WISP1tc, WISP1c, WISP1i, WISP-1, WISP1.

Introduction

WISP1 is a part of a family of cysteine-rich, glycosylated signaling proteins that mediate varied developmental processes. WISP1 is a Downstream regulator in the Wnt/Frizzled-signaling pathway and is linked with cell survival.WISP1weakens p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X protein.

WISP1 是富含半胱氨酸的糖基化信号蛋白家族的一部分，可介导不同的发育过程。 WISP1 是 Wnt/Frizzled 信号通路的下游调节因子，与细胞存活有关。WISP1 通过激活 AKT 激酶减弱 p53 介导的细胞凋亡以响应 DNA 损伤，并上调抗凋亡 Bcl-X 蛋白。

Description

WISP1 Human Recombinant produced in Mouse myeloma cell line is a single, glycosylated polypeptide chain containing 355 amino acids , having a molecular mass of 39.3kDa and fused to a 10 aa His Tag.

The WISP1 is purified by proprietary chromatographic techniques.

在小鼠骨髓瘤细胞系中产生的 WISP1 Human Recombinant 是一条含有 355 个氨基酸的糖基化多肽链，分子量为 39.3kDa，并与 10 个氨基酸的 His 标签融合。

WISP1 通过专有的色谱技术进行纯化。

Source

HEK293 Cells.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

WISP1 protein was lyophilized from a 0.2µm filtered solution in PBS.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

TALSPAPTTMDFTPAPLEDTSSRPQFCKWPCECPPSPPRCPLGVSLITDGCECCKMCAQQLGDNCTEAAICDPHRG

LYCDYSGDRPRYAIGVCAQVVGVGCVLDGVRYNNGQSFQPNCKYNCTCIDGAVGCTPLCLRVRPPRLWCPHPRR

VSIPGHCCEQWVCEDDAKRPRKTAPRDTGAFDAVGEVEAWHRNCIAYTSPWSPCSTSCGLGVSTRISNVNAQCW

PEQESRLCNLRPCDVDIHTLIKAGKKCLAVYQPEASMNFTLAGCISTRSYQPKYCGVCMDNRCCIPYKSKTIDVSFQC

PDGLGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFSEIANHHHHHHHHHH.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Prospec 的产品仅供实验室研究使用。它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-VEGF (121 a.a.) Human

Description

Catalogue number

CYT-343

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types .VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesisand cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Description

Vascular Endothelial Growth Factor-121 Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide double chain containing 2×121 amino acids and having a molecular mass of 28.4kDa. VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a concentrated solution with no additives.

Solubility

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor -121 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Vascular Endothelial Growth Factor 121 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-121 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R

Biological Activity

VEGF-121 has full biological activity when compared to standards. The activity is determined by the dose-dependent proliferation of HUVECs and is typically 1-6ng/ml corresponding to a specific activity of 166,667-1,000,000U/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-WISP2 Human/CYT-970

ProSpec-WISP2 Human,ProSpec CYT-970,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,WNT1 Inducible Signaling Pathway Protein 2, Connective Tissue Growth Factor-Related Protein 58, Connective Tissue Growth Factor-Like Protein, CCN Family Member 5, CTGF-L, CT58, CCN5, WISP-2, CTGFL, WISP2.

WISP2 Human

WNT1 Inducible Signaling Pathway Protein 2 Human Recombinant
CYT-970
ProSpec-WISP2 Human/CYT-970 5µg
ProSpec-WISP2 Human/CYT-970 20µg
ProSpec-WISP2 Human/CYT-970 1mg

Catalogue number

CYT-970

Synonyms

WNT1 Inducible Signaling Pathway Protein 2, Connective Tissue Growth Factor-Related Protein 58, Connective Tissue Growth Factor-Like Protein, CCN Family Member 5, CTGF-L, CT58, CCN5, WISP-2, CTGFL, WISP2.

Introduction

WNT1-inducible-signaling pathway protein 2 belongs to the WNT1 inducible signaling pathway protein subfamily, which belongs to the connective tissue growth factor family. The CTGF family members are characterized by 4 conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. WISP2 protein lacks the CT domain which is implicated in dimerization binding. WISP2 is possibly involved in bone remodeling. WISP2 is expressed in primary osteoblasts and fibroblasts. WISP2 stimulates osteoblast adhesion and inhibits osteocalcin production. WISP2 expression in colon tumors is reduced while the other 2 WISP members are overexpressed in colon tumors. WISP2 may play an imperative role in modulating bone turnover.

WNT1诱导信号通路蛋白2属于WNT1诱导信号通路蛋白亚家族，属于结缔组织生长因子家族。 CTGF 家族成员的特征在于 4 个保守的富含半胱氨酸结构域：胰岛素样生长因子结合结构域、von Willebrand 因子 C 型模块、血小板反应蛋白结构域和 C 末端胱氨酸结样结构域。 WISP2 蛋白缺乏与二聚化结合有关的 CT 结构域。 WISP2 可能参与骨重塑。 WISP2 在原代成骨细胞和成纤维细胞中表达。 WISP2 刺激成骨细胞粘附并抑制骨钙素的产生。 WISP2 在结肠肿瘤中的表达减少，而其他 2 个 WISP 成员在结肠肿瘤中过度表达。 WISP2 可能在调节骨转换中发挥重要作用。

Description

WISP2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing a total of 228 amino acids and having a molecular mass of 24.4kDa.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid .

Solubility

It is recommended to reconstitute the lyophilized WISP-2 in sterile 10mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized WISP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution WISP-2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 WISP2 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。重构后，WISP-2 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF (121 a.a.) Human, Sf9

Description

Catalogue number

CYT-200

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction

Description

Vascular Endothelial Growth Factor-121 Human Recombinant produced in insect cells as an 18kDa homodimer, is a glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of approximately 36kDa.
VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.
The VEGF-121 is purified by proprietary chromatographic techniques.

Source

Sf9, Insect Cells.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a solution containing 50mM acetic acid.

Solubility

The lyophilized VEGF121 should be reconstituted in 50mM acetic acid to a concentration not lower than 50µg/ml.

Stability

Purity

Greater than 95.0% as determined by -PAGE.

Biological Activity

Measured in a cell proliferation assay using primary HUVECs. The ED₅₀ for this effect is typically 2-10ng/ml.

Amino acid sequence

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPS CVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHN KCECRPKKDRARQEKCDKPRR.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-WNT3A Human/CYT-861

ProSpec-WNT3A Human,ProSpec CYT-861,在大肠杆菌中产生的 WNT3A 人重组体是一条单一的、非糖基化的多肽链，含有 335 个氨基酸，分子量为 37.5kDa。 WNT3A Human 通过专有的色谱技术进行纯化。

WNT3A Human

Wingless-Type MMTV Integration Site Family, Member 3A Human Recombinant
CYT-861
ProSpec-WNT3A Human/CYT-861 5µg
ProSpec-WNT3A Human/CYT-861 20µg
ProSpec-WNT3A Human/CYT-861 1mg

Catalogue number

CYT-861

Synonyms

Protein Wnt-3a, MGC119418, MGC119419, MGC119420, WNT3A, wingless-type MMTV integration site family member 3A.

Introduction

The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins are implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT3A is a member of the WNT gene family. WNT3A is a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. The WNT3A gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region.

WNT 基因家族由编码分泌信号蛋白的结构相关基因组成。这些蛋白质与肿瘤发生和几个发育过程有关，包括在胚胎发生过程中调节细胞命运和模式。 WNT3A 是 WNT 基因家族的成员。 WNT3A 是一种蛋白质，其与小鼠 Wnt3A 蛋白具有 96% 的氨基酸同一性，与人类 WNT3 蛋白（另一种 WNT 基因产物）具有 84% 的氨基酸同一性。 WNT3A基因与另一个家族成员WNT14基因聚集在染色体1q42区域。

Description

WNT3A Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 335 amino acids and having a molecular mass of 37.5kDa. The WNT3A Human is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 WNT3A 人重组体是一条单一的、非糖基化的多肽链，含有 335 个氨基酸，分子量为 37.5kDa。 WNT3A Human 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The WNT3A Human protein solution contains 20mM Tris-HCl buffer and 10% glycerol.

Stability

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

MSYPIWWSLA VGPQYSSLGS QPILCASIPG LVPKQLRFCR NYVEIMPSVA EGIKIGIQEC QHQFRGRRWN CTTVHDSLAI FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTAAICG CSSRHQGSPG KGWKWGGCSE DIEFGGMVSR EFADARENRP DARSAMNRHN NEAGRQAIAS HMHLKCKCHG LSGSCEVKTC WWSQPDFRAI GDFLKDKYDS ASEMVVEKHR ESRGWVETLR PRYTYFKVPT ERDLVYYEAS PNFCEPNPET GSFGTRDRTC NVSSHGIDGC DLLCCGRGHN ARAERRREKC RCVFHWCCYV SCQECTRVYD VHTCK.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF (121 a.a.) Mouse

Description

Catalogue number

CYT-574

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types .VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Description

Vascular Endothelial Growth Factor-121 Mouse Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 28.4 kDa.
Recombinant Mouse VEGF-121 is a truncated version of Murine VEGF-165.
The VEGF-121 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a concentrated solution with no additives.

Solubility

It is recommended to reconstitute the lyophilized VEGF-121 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized VEGF-121 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-121 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKRPR R.

References

Title:VEGF signaling mediates bladder neuroplasticity and inflammation in response to BCG.
Publication:BMC Physiology 2011, 11:16 doi:10.1186/1472-6793-11-16
Link:http://www.biomedcentral.com/1472-6793/11/16/

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-WNT7A Human/CYT-795

ProSpec-WNT7A Human/CYT-795,在大肠杆菌中产生的 WNT7A 人重组体是一条单一的、非糖基化的多肽链，包含 341 个氨基酸，分子量为 38kDa。WNT7A 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

WNT7A Human

Wingless-Type MMTV Integration Site Family, Member 7A Human Recombinant
CYT-795
ProSpec-WNT7A Human/CYT-795 5µg
ProSpec-WNT7A Human/CYT-795 20µg
ProSpec-WNT7A Human/CYT-795 1mg

Catalogue number

CYT-795

Synonyms

Wingless-Type MMTV Integration Site Family, Member 7A, Proto-Oncogene Wnt7a Protein, Protein Wnt-7a.

Introduction

Wingless-Type MMTV Integration Site Family, Member 7A also known as WNT7A belongs to the WNT gene family, this family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT7A is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays an essential role in uterine smooth muscle pattering and maintenance of adult uterine function. In addition, Mutations in WNT7A have been associated with Fuhrmann and Al-Awadi Raas-Rothschild Schinzel phocomelia syndromes.

Wingless-Type MMTV 整合位点家族，成员 7A 也称为 WNT7A，属于 WNT 基因家族，该家族由编码分泌信号蛋白的结构相关基因组成。这些蛋白质与肿瘤发生和几个发育过程有关，包括在胚胎发生过程中调节细胞命运和模式。 WNT7A 参与女性生殖道前后轴的发育，在子宫平滑肌模式和成人子宫功能的维持中也发挥着重要作用。此外，WNT7A 的突变与 Fuhrmann 和 Al-Awadi Raas-Rothschild Schinzel phocomelia 综合征有关。

Description

WNT7A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 341 amino acids and having a molecular mass of 38kDa.
WNT7A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 WNT7A 人重组体是一条单一的、非糖基化的多肽链，包含 341 个氨基酸，分子量为 38kDa。
WNT7A 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

WNT7A protein solution containing 20mM Tris-HCl buffer , 10% glycerol and 0.4M Urea.

Stability

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSLGASIIC NKIPGLAPRQ RAICQSRPDA IIVIGEGSQM GLDECQFQFR NGRWNCSALG ERTVFGKELK VGSREAAFTY AIIAAGVAHA ITAACTQGNL SDCGCDKEKQ GQYHRDEGWK WGGCSADIRY GIGFAKVFVD AREIKQNART LMNLHNNEAG RKILEENMKL ECKCHGVSGS CTTKTCWTTL PQFRELGYVL KDKYNEAVHV EPVRASRNKR PTFLKIKKPL SYRKPMDTDL VYIEKSPNYC EEDPVTGSVG TQGRACNKTA PQASGCDLMC CGRGYNTHQY ARVWQCNCKF HWCCYVKCNT CSERTEMYTC K.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-VEGF (121a.a.) Human, HEK

Description

Catalogue number

CYT-116

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction

Description

Recombinant Human VEGF 121 amino acids, produced in HEK cells is a glycosylated 37kDa homodimer and 50kDa homotrimer.
The VEGF is purified by proprietary chromatographic techniques.

Source

HEK.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

VEGF was lyophilized from a 0.2µm filtered solution containing 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized VEGF in sterile PBS containing 0.1% endotoxin-free recombinant HSA.

Stability

Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 95% as obsereved by -PAGE.

Biological Activity

The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells , the ED₅₀ is 3ng/ml.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-AIMP1 Human/CYT-021

ProSpec-AIMP1 Human,ProSpec CYT-021,Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43, AIMP1, EMAP2, SCYE1, p43, EMAPII.

AIMP1 Human
Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1 Human Recombinant
CYT-021
ProSpec-AIMP1 Human/CYT-021 1µg
ProSpec-AIMP1 Human/CYT-021 5µg
ProSpec-AIMP1 Human/CYT-021 50µg

Catalogue number

CYT-021

Synonyms

Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43, AIMP1, EMAP2, SCYE1, p43, EMAPII.

Introduction

AIMP1 is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of the AIMP1 cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. Furthermore, AIMP1 is involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells.

AIMP1 是一种由细胞凋亡特异性诱导的细胞因子，它参与控制血管生成、炎症和伤口愈合。 AIMP1 细胞因子的释放使肿瘤相关脉管系统对肿瘤坏死因子敏感。此外，AIMP1 参与在肿瘤细胞中蛋白水解切割后刺激炎症反应。

Description

AIMP1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 356 amino acids and having a molecular mass of 39.2kDa . The AIMP1 is purified by proprietary chromatographic techniques.

AIMP1 Human Recombinant 在 N 端融合了 20 个氨基酸的 His 标签，在大肠杆菌中产生，是一个单一的、非糖基化的多肽链，包含 356 个氨基酸，分子量为 39.2kDa。 AIMP1 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The AIMP1 solution 20mM Tris-HCl buffer , 0.2M NaCl, 2mM DTT and 10% glycerol.

Stability

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
请避免冻融循环。

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MLPAVAVSEP VVLRFMIFCR LLAKMANNDA VLKRLEQKGA EADQIIEYLK QQVSLLKEKA ILQATLREEK KLRVENAKLK KEIEELKQEL IQAEIQNGVK QIPFPSGTPL HANSMVSENV IQSTAVTTVS SGTKEQIKGG TGDEKKAKEK IEKKGEKKEK KQQSIAGSAD SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF C Human

Description

Catalogue number

CYT-527

Synonyms

VEGF-C, Vascular endothelial growth factor C, VRP, Flt4 ligand, Flt4-L, Vascular endothelial growth factor-related protein, VEGFC.

Introduction

VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein , is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. Human VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant human VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant human VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A.

Description

VEGF-C Human Recombinant- contains 121 amino acids residues including 6 amino acid His-tag fused at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in -PAGE under reducing conditions.

Source

Sf9, Insect Cells.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Each mg of VEGF-C Human contains 50mg BSA and 1xPBS as buffer.

Solubility

It is recommended to reconstitute the lyophilized VEGF-C in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized VEGF-C although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-C should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Biological Activity

Measured by its ability to induce to the VEGFR-3/FLT-4 receptor phosphorylation inPAEC/VEGFR3 cells and VEGFC induced proliferation of primary HDLEC cells.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-CLCF1 Human/CYT-071

ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,ProSpec-CLCF1 Human,ProSpec CYT-071,Cardiotrophin-like cytokine factor 1, B-cell-stimulating factor 3, BSF-3, Novel neurotrophin-1, NNT-1, CLCF1, BSF3, CLC, NNT1, NR6, CISS2.

CLCF1 Human

Cardiotrophin-Like Cytokine Factor 1 Human Recombinant
CYT-071
ProSpec-CLCF1 Human/CYT-071 5µg
ProSpec-CLCF1 Human/CYT-071 20µg
ProSpec-CLCF1 Human/CYT-071 1mg

Catalogue number

CYT-071

Synonyms

Cardiotrophin-like cytokine factor 1, B-cell-stimulating factor 3, BSF-3, Novel neurotrophin-1, NNT-1, CLCF1, BSF3, CLC, NNT1, NR6, CISS2.

Introduction

Cardiotrophin-like cytokine factor 1 is a member of the interleukin 6 family of cytokines, which are involved in cell signaling via phosphorylation of gp130. CLCF1 has a sequence of 225 amino acids with a 27 aa signal peptide, having a molecular mass of 22kDa in the mature form, and the maximum homology to cardiotrophin-1 and ciliary neurotrophic factor. CLCF1 is actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. Defects in the CLCF1 gene cause cold-induced sweating syndrome 2 . The CISS2 syndrome is typified by profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine.

心肌营养素样细胞因子因子 1 是白细胞介素 6 细胞因子家族的成员，其通过 gp130 的磷酸化参与细胞信号传导。 CLCF1具有225个氨基酸的序列，带有27个氨基酸的信号肽，成熟形式的分子量为22kDa，与cardiotrophin-1和睫状神经营养因子同源性最大。 CLCF1 通过与可溶性 I 型 CRLF1 或可溶性 CNTFR 形成复合物从细胞中主动分泌。 CLCF1 基因的缺陷会导致感冒出汗综合征 2 。 CISS2综合征的典型特征是暴露于寒冷后大量出汗以及头部和脊柱的先天性身体异常。

Description

CLCF1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids and having a molecular mass of 24.6kDa.
CLCF1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 CLCF1 Human Recombinant 是单条非糖基化多肽链，含有 219 个氨基酸，分子量为 24.6kDa。
CLCF1 在 N 端与 21 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

CLCF1 protein solution containing 20mM sodium citrate , 0.4M Urea and 10% glycerol.

Stability

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF C Rat

Description

Catalogue number

CYT-262

Synonyms

VEGF-C, Vascular endothelial growth factor C, VRP, Flt4 ligand, Flt4-L.

Introduction

VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein , is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGFC cDNA encodes a pre-pro-protein of 416 amino acids residues.
It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT -4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A.

Description

Vascular Endothelial Growth Factor C Rat Recombinant contains 127 amino acids residues and was fused to a His- tag at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 15-20 kDa protein in -PAGE under reducing conditions.

Source

Sf9, Insect Cells.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Each mg of VEGF-C Rat contains 50mg BSA and PBS as buffer.

Solubility

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor C in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Vascular Endothelial Growth Factor-C although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-C should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

DTVKLAAAHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGAATNTFFKP PCVSVYRCGGCCNSEGLQCMNTSTGYLSKTLFEITVPLSQGPKPVTISFA NHTSCRCMSKLDVYRQVHSIIHHHHHH.

Biological Activity

Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells. The ED50 for this effect is typically 200-300ng/ml corresponding to a Specific Activity of 3,334-5,000IU/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-CYTL1 Human/CYT-775

ProSpec-CYTL1 Human,ProSpec CYT-775,细胞因子样蛋白 1 是一种分泌蛋白。 CYTL1 在作为造血干细胞/祖细胞的骨髓和脐带血的 CD34+ 细胞群中表达。然而，CYTL1 不在 CD34- 细胞中表达。对敲除小鼠的实验表明，CYTL1 对维持软骨稳态而不是软骨和骨骼发育至关重要，并且 CYTL1 功能的丧失与小鼠实验性骨关节炎软骨破坏有关。

CYTL1 Human

Cytokine-Like 1 Human Recombinant
CYT-775
ProSpec-CYTL1 Human/CYT-775 2µg
ProSpec-CYTL1 Human/CYT-775 10µg
ProSpec-CYTL1 Human/CYT-775 1mg

Catalogue number

CYT-775

Synonyms

Cytokine-like protein 1, Protein C17, CYTL1, C4orf4, C17.

Introduction

Cytokine-like protein 1 is a secreted protein. CYTL1 is expressed in CD34+ cell populations of bone marrow and cord blood which function as hematopoietic stem/progenitor cells. However, CYTL1 is not expressed in CD34- cells. Experiments with knock-out mice propose that CYTL1 is essential for the maintenance of cartilage homeostasis rather than cartilage and bone development, and loss of CYTL1 function is linked with experimental osteoarthritic cartilage destruction in mice.

细胞因子样蛋白 1 是一种分泌蛋白。 CYTL1 在作为造血干细胞/祖细胞的骨髓和脐带血的 CD34+ 细胞群中表达。然而，CYTL1 不在 CD34- 细胞中表达。对敲除小鼠的实验表明，CYTL1 对维持软骨稳态而不是软骨和骨骼发育至关重要，并且 CYTL1 功能的丧失与小鼠实验性骨关节炎软骨破坏有关。

Description

CYTL1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 124 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 14.6kDa .

在大肠杆菌中产生的 CYTL1 Human Recombinant 是一个单一的、非糖基化的多肽链，包含 124 个氨基酸，包括一个 10 个氨基酸的 N 端 His 标签。总分子量为14.6kDa。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

CYTL1 was filtered and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl, pH 7.5.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. CYTL1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Stability

将冻干蛋白储存在 -20°C。重组后分装产品以避免重复的冷冻/解冻循环。重组蛋白可在 4°C 下保存一段时间；在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MKHHHHHHASTPPTCYSRMR ALSQEITRDF NLLQVSEPSE PCVRYLPRLY LDIHNYCVLD KLRDFVASPP CWKVAQVDSL KDKARKLYTI MNSFCRRDLV FLLDDCNALE YPIPVTTVLP DRQR.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF C Rat (152 a.a.)

Description

Catalogue number

CYT-284

Synonyms

VEGF-C152, Vascular endothelial growth factor C 152, VRP, Flt4 ligand, Flt4-L.

Introduction

VEGF-C152S is a point mutant generated by the replacement of the second conserved Cys residue of the recombinant processed VEGF-C by a Ser residue. VEGF-C 152S is analog to the human VEGF-C 156S mutant and only active toward VEGFR-3/FLT-4 but, unlike wild type VEGF-C, is unable to bind to and to activate signaling through VEGFR-2/KDR. VEGF-C152S was inactive in the vascular permeability assay and did not increase migration of the capillary endothelial cells, indicating that these VEGF-like effects of VEGF-C require VEGFR-2 binding. VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein , is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT -4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A.

Description

Vascular Endothelial Growth Factor -C 152 Rat Recombinant contains 152 amino acids residues and was fused to a His-tag at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in -PAGE under reducing conditions.

Source

Sf9, Insect Cells.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a concentrated solution with BSA.

Solubility

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor C 152 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Vascular Endothelial Growth Factor-C152 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-C 152 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 90.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Biological Activity

Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells . The ED50 for this effect is typically 150-300 ng/ml, corresponding to a specific activity of 3.3-6.6KUnits/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-EMAP II Human/CYT-607

ProSpec-EMAP II Human,ProSpec CYT-607,EMAP-II 也称为 SCYE1 是一种源自肿瘤的细胞因子，它在内皮细胞、单核细胞和中性粒细胞的多种活动中发挥作用。 EMAP-II 抑制内皮细胞增殖、血管生成、新血管形成，并可诱导细胞凋亡。它还对嗜中性粒细胞和单核细胞具有趋化性，并诱导嗜中性粒细胞的髓过氧化物酶活性。

EMAP II Human

Endothelial-Monocyte Activating Polypeptide II Human Recombinant
CYT-607
ProSpec-EMAP II Human/CYT-607 5µg
ProSpec-EMAP II Human/CYT-607 20µg
ProSpec-EMAP II Human/CYT-607 1mg

Catalogue number

CYT-607

Synonyms

AIMP1, EMAP2, EMAP-2, EMAPII, SCYE1, Multisynthetase complex auxiliary component p43, Endothelial monocyte-activating polypeptide 2, EMAP-II, p43.

Introduction

EMAP-II also called SCYE1 is a tumor derived cytokine that plays a role in a wide variety of activities on endothelial cells, monocytes and neutrophils. EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel formation, and can induce apoptosis. It is also chemotactic towards neutrophils and monocytes and induces myeloperoxidase activity from neutrophils. EMAP-II clinical value is inhibiting angiogenesis of vascular beds and suppressing the growth of primary and secondary tumors with no affect to normal tissues. SCYE1is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this SCYE1 renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. EMAP-2 plays a role in in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells.

EMAP-II 也称为 SCYE1 是一种源自肿瘤的细胞因子，它在内皮细胞、单核细胞和中性粒细胞的多种活动中发挥作用。 EMAP-II 抑制内皮细胞增殖、血管生成、新血管形成，并可诱导细胞凋亡。它还对嗜中性粒细胞和单核细胞具有趋化性，并诱导嗜中性粒细胞的髓过氧化物酶活性。 EMAP-II的临床价值是抑制血管床的血管生成，抑制原发性和继发性肿瘤的生长，对正常组织无影响。 SCYE1 由细胞凋亡特异性诱导，参与控制血管生成、炎症和伤口愈合。这种 SCYE1 的释放使肿瘤相关脉管系统对肿瘤坏死因子敏感。前体蛋白与 p43 亚基相同，与多 tRNA 合成酶复合物相关，它调节正常细胞中 tRNA 合成酶的氨酰化活性。 EMAP-2 在肿瘤细胞蛋白水解切割后刺激炎症反应中起作用。

Description

EMAP-II Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 18.3 kDa.
The EMAP-II is purified by proprietary chromatographic techniques.

EMAP-II Recombinant Human 在大肠杆菌中产生，是一条单一的、非糖基化的多肽链，含有 166 个氨基酸，分子量为 18.3 kDa。
EMAP-II 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a concentrated solution in water containing 20mM sodium Phosphate buffer pH=7.5 and 130mM sodium chloride.

Solubility

It is recommended to reconstitute the lyophilized EMAP-II in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 EMAP-II，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized EMAP-II although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EMAP-II should be stored at 4°C between 2-7 days and for future use below
-18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQM QNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK.

Biological Activity

Determined by the apoptotic effect on MCF-7 cells using a concentration of 20-30 ng/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-VEGF D Human

Description

Catalogue number

CYT-045

Synonyms

c-fos induced growth factor , FIGF, VEGFD.

Introduction

VEGF-D belongs to the VEGF/PDGF family of proteins. VEGF-D promotes lymphangiogesis, endothelial cell growth, and regulates vascular permeability. In addition, VEGF-D has an important part in the creation of the venous and lymphatic vascular systems and in the growth and maintenance of differentiated lymphatic endothelium Mature VEGF-D forms a noncovalently linked homodimer, and binds to and activate both VEGFR-2 and VEGFR-3 .

Description

VEGFD Human Recombinant produced in HEK-293 cells is a secreted protein fused to a polyhistidine tag at the C-terminus.

Source

HEK293.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The recombinant VEGF-D was lyophilized after extensive dialysis against PBS.

Solubility

It is recommended to reconstitute the Vascular Endothelial Growth Factor D in sterile 18M-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized VEGF-D although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-D should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Biological Activity

The ED50 of 3-4ng/ml is measured by its ability to stimulate the proliferation of human microvascular endothelial cells .

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF E (Orf Virus)

Description

Catalogue number

CYT-263

Introduction

Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus . Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins . Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E can not bind to VEGF receptor-1 . Furthermore VEGF-E can also not bind to VEGF receptor-3 . Therefore VEGF-E is a potent angiog

Description

A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a concentrated solution containing PBS.

Solubility

The lyophilized oVEGF-E Orf Virus should be reconstituted in water or medium to a concentration not lower than 50µg/ml. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin.

Stability

Lyophilized Vascular Endothelial Growth Factor-E Orf Virus although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF E -OV should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Biological Activity

The biological activity was determined by the ability to induce VEGFR-2/KDR receptor phosphorylation in PAE/KDR cells and in a cell proliferation assay using primary HUVECs. The ED₅₀ for this effect is typically 1-5ng/ml.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH DSTKTWSEVF ENSGCKPRPM VFRVHDEHPE LTSQRFNPPC VTLMRCGGCC NDESLECVPT EEANVTMQLM GASVSGGNGM QHLSFVEHKK CDCKPPLTTT PPTTTRPPRR RR

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF Equine

Description

Catalogue number

CYT-1200

Synonyms

VEGF-A, VPF, glioma-derived endothelial cell mitogen.

Introduction

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types .VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Description

VEGF Equine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 2 x 165 amino acids and having a total molecular mass of 38.6 kDa.
The VEGF is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a sterile filtered aqueous solution containing 10 mM Sodium Phosphate, pH 7.5.

Solubility

It is recommended to reconstitute the lyophilized VEGF in sterile water at a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF Human should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MAPMAEGEHK THEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TAEFNITMQI MRIKPHQSQH IGEMSFLQHS KCECRPKKDK ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Biological Activity

The ED₅₀ as determined by HUVEC Proliferation is ≤ 10 ng/mL, corresponding to a specific activity of ≥ 1.0 x 10^5 units/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF Human

Description

Catalogue number

CYT-241

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction

Vascular endothelial growth factor is an important signaling protein involved in vessel formation As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types . VEGF mediates increased vascular permeability, induces vasculogenesis and endothelial cell production, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. VEGF is located in normal cartilage though only osteoarthritic cartilage expresses the VEGF receptors, NP1, VEGFR1 and VEGFR2. The VEGF level in the culture media from OA chondrocytes was more than 3 folds higher than in media from normal chondrocytes

Description

Vascular Endothelial Growth Factor Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 38.2kDa.
The VEGF is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The VEGF protein was lyophilized from a concentrated solution with no additives.

Solubility

It is recommended to reconstitute the lyophilized VEGF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Biological Activity

Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells using a concentration range of 3.7-5.6 ng/ml, corresponding to a Specific Activity of 178,570-270,270IU/mg.

Protein content

VEGF protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

References

1. Title:Expression of VEGF and Its Receptors in the Bovine Endometrium Throughout the Estrous Cycle: Effects of VEGF on Prostaglandin Production in Endometrial Cells.
Publication:Journal of Reproduction and Development
Vol. 56 No. 2 April P 223-229
Link:https://www.jstage.jst.go.jp/article/jrd/56/2/56_09-139S/_article

2. Title: Cardio-specific long-term gene expression in a porcine model after selective pressure-regulated retroinfusion of adeno-associated viral vectors
Publications: Gene therapy 15.1 : 12-17.
Link: http://www.nature.com/gt/journal/v15/n1/full/3303035a.html

3. Title: SU11248, A selective tyrosine kinases inhibitor suppresses breast tumor angiogenesis and growth via targeting both tumor vasculature and breast cancer cells
Publication: Cancer biology & therapy 10.7 : 703-711.
Link: http://www.tandfonline.com/doi/full/10.4161/cbt.10.7.12904

ProSpec-VEGF Human (121 a.a.), His

Description

Catalogue number

CYT-619

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types .VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Description

Vascular Endothelial Growth Factor-121 Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain containing a total of 142 amino acids and having a molecular mass of 16.3 kDa.
The VEGF-121 is fused to a 20 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

VEGF-121 His Tag in 20mM Tris pH-8 and 10% glycerol.

Stability

Purity

Greater than 90.0% as determined by -PAGE.

Biological Activity

The ED50 for this effect is <4.2 ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell, corresponding to a specific activity of less than 238,095.23units/mg.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF Human, Baculovirus

Description

Catalogue number

CYT-849

Synonyms

Vascular Endothelial Growth Factor A, VEGF, Vascular Permeability Factor, MVCD1, VPF, Vascular Endothelial Growth Factor, VEGF-A, Vascular endothelial growth factor A.

Introduction

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types .VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesisand cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Description

VEGF produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 197 amino acids and having a molecular mass of 23.1 kDa.

VEGF is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

VEGF protein solution contains Phosphate buffered saline , 10% glycerol, 1mM DTT and 0.1mM PMSF.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.

Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

MNFLLSWVHW SLALLLYLHH AKWSQAAPMA EGGGQNHHEV VKFMDVYQRS YCHPIETLVD IFQEYPDEIE YIFKPSCVPL MRCGGCCNDE GLECVPTEES NITMQIMRIK PHQGQHIGEM SFLQHNKCEC RPKKDRARQE NPCGPCSERR KHLFVQDPQT CKCSCKNTDS RCKARQLELN ERTCRCDKPR RHHHHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF Human, CHO

Description

Catalogue number

CYT-260

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types . VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesisand cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Description

Vascular Endothelial Growth Factor Human Recombinant produced in CHO cells is a double, glycosylated, polypeptide chain containing 165 amino acids and migrates as 44 kDa in -PAGE under non-reducing conditions.
The VEGF is purified by proprietary chromatographic techniques.

Source

Chinese Hamster Ovarian Cell.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a Phosphate- Buffered Saline, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor Human in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Vascular Endothelial Growth Factor Human although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF Human Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 97.0% as determined by -PAGE.

Biological Activity

The protein was tested in HUVEC cells, the ED50 for this effect was found to be 2-6ng/ml.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-IL6ST Human/CYT-1156

ProSpec-IL6ST Human,ProSpec CYT-1156,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,在 Sf9 杆状病毒细胞中产生的 IL6ST Human 是单条糖基化多肽链，含有 605 个氨基酸，分子量为 68.9kDa。IL6ST 在 C 末端与 8 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

IL6ST Human
Interleukin-6 Signal Transducer Human Recombinant
CYT-1156
ProSpec-IL6ST Human/CYT-1156 1µg
ProSpec-IL6ST Human/CYT-1156 5µg
ProSpec-IL6ST Human/CYT-1156 50µg

Catalogue number

CYT-1156

Synonyms

Interleukin 6 signal transducer, oncostatin M receptor, IL6ST, CD130, CDw130, GP130, GP130-RAPS, IL6R-beta

Introduction

Interleukin-6 Signal Transducer or IL6ST is a receptor, part of the family of class 1 cytokine receptor. IL6ST binds to IL-6 through membrane-anchored or soluble IL-6R starts a connection between another complex of IL6ST and IL-6 , thus forming a homo-dimer and a signal transduction occurs.

Interleukin-6 Signal Transducer 或 IL6ST 是一种受体，是 1 类细胞因子受体家族的一部分。 IL6ST通过膜锚定或可溶性IL-6R与IL-6结合，启动IL6ST和IL-6的另一个复合物之间的连接，从而形成同源二聚体并发生信号转导。

Description

IL6ST Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 605 amino acids and having a molecular mass of 68.9kDa.
IL6ST is fused to an 8 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 IL6ST Human 是单条糖基化多肽链，含有 605 个氨基酸，分子量为 68.9kDa。
IL6ST 在 C 末端与 8 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The IL6ST solution contains 10% Glycerol and Phosphate-Buffered Saline .

Stability

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

ELLDPCGYIS PESPVVQLHS NFTAVCVLKE KCMDYFHVNA NYIVWKTNHF TIPKEQYTII NRTASSVTFT DIASLNIQLT CNILTFGQLE QNVYGITIIS GLPPEKPKNL SCIVNEGKKM RCEWDRGRET HLETNFTLKS EWATHKFADC KAKRDTPTSC TVDYSTVYFV NIEVWVEAEN ALGKVTSDHI NFDPVYKVKP NPPHNLSVIN SEELSSILKL TWTNPSIKSV IILKYNIQYR TKDASTWSQI PPEDTASTRS SFTVQDLKPF TEYVFRIRCM KEDGKGYWSD WSEEASGITY EDRPSKAPSF WYKIDPSHTQ GYRTVQLVWK TLPPFEANGK ILDYEVTLTR WKSHLQNYTV NATKLTVNLT NDRYVATLTV RNLVGKSDAA VLTIPACDFQ ATHPVMDLKA FPKDNMLWVE WTTPRESVKK YILEWCVLSD KAPCITDWQQ EDGTVHRTYL RGNLAESKCY LITVTPVYAD GPGSPESIKA YLKQAPPSKG PTVRTKKVGK NEAVLEWDQL PVDVQNGFIR NYTIFYRTII GNETAVNVDS SHTEYTLSSL TSDTLYMVRM AAYTDEGGKD GPEFTFTTPK FAQGEIELEH HHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-VEGF Human, HEK

Description

Catalogue number

CYT-225

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction

Description

Vascular Endothelial Growth Factor Human Recombinant produced in HEK293 cells is a double, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 40 kDa. The VEGF is purified by proprietary chromatographic techniques.

Source

HEK293 .

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a 0.2µM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Solubility

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor-HEK in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Vascular Endothelial Growth Factor HEK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF HEK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein . Please prevent freeze-thaw cycles.

Amino acid sequence

APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Purity

Greater than 95.0% as determined by -PAGE.

Biological Activity

Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells using a concentration range of 4.0ng/ml, corresponding to a specific activity of 2.5 x 10⁵ Units/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-NTRK2 Human/CYT-1159

ProSpec-NTRK2 Human,ProSpec CYT-1159,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines.神经营养受体酪氨酸激酶 2，也称为 NTRK2，是一种酪氨酸蛋白激酶受体，通过调节神经元存活、迁移、增殖、分化和突触形成，参与中枢和外周神经系统的发育和成熟。可塑性。 NTRK2 通过调节短期突触功能和长期增强作用在学习和记忆中。 TRK 家族受体的已知底物是：SHC1、PI-3 激酶和 PLC-γ-1。

NTRK2 Human
Neurotrophic Receptor Tyrosine Kinase 2 Human Recombinant
CYT-1159
ProSpec-NTRK2 Human/CYT-1159 2µg
ProSpec-NTRK2 Human/CYT-1159 10µg
ProSpec-NTRK2 Human/CYT-1159 1mg

Catalogue number

CYT-1159

Synonyms

GP145-TrkB, trk-B, TRKB, NTRK2

Introduction

Neurotrophic Receptor Tyrosine Kinase 2, also referred to NTRK2, is a tyrosine-protein kinase receptor which takes part in the development & the maturation of the central and the peripheral nervous systems through regulation of neuron survival, migration, proliferation, differentiation and synapse formation & plasticity. NTRK2 acts in learning and memory by regulating both short term synaptic function and long-term potentiation. The substrates that are known for the TRK family receptors are: SHC1, PI-3 kinase and PLC-gamma-1.

神经营养受体酪氨酸激酶 2，也称为 NTRK2，是一种酪氨酸蛋白激酶受体，通过调节神经元存活、迁移、增殖、分化和突触形成，参与中枢和外周神经系统的发育和成熟。可塑性。 NTRK2 通过调节短期突触功能和长期增强作用在学习和记忆中。 TRK 家族受体的已知底物是：SHC1、PI-3 激酶和 PLC-γ-1。

Description

NTRK2 Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 407 amino acids and having a molecular mass of 45.2kDa.
NTRK2 is fused to an 8 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 NTRK2 Human 是一条糖基化多肽链，包含 407 个氨基酸，分子量为 45.2kDa。
NTRK2 在 C 末端与 8 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The NTRK2 solution contains 10% Glycerol and Phosphate-Buffered Saline .

Stability

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

CPTSCKCSAS RIWCSDPSPG IVAFPRLEPN SVDPENITEI FIANQKRLEI INEDDVEAYV GLRNLTIVDS GLKFVAHKAF LKNSNLQHIN FTRNKLTSLS RKHFRHLDLS ELILVGNPFT CSCDIMWIKT LQEAKSSPDT QDLYCLNESS KNIPLANLQI PNCGLPSANL AAPNLTVEEG KSITLSCSVA GDPVPNMYWD VGNLVSKHMN ETSHTQGSLR ITNISSDDSG KQISCVAENL VGEDQDSVNL TVHFAPTITF LESPTSDHHW CIPFTVKGNP KPALQWFYNG AILNESKYIC TKIHVTNHTE YHGCLQLDNP THMNNGDYTL IAKNEYGKDE KQISAHFMGW PGIDDGANPN YPDVIYEDYG TAANDIGDTT NRSNEIPSTD VTDKTGREHL EHHHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF Human, His

Description

Catalogue number

CYT-496

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction

Description

Vascular Endothelial Growth Factor Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing a total of 185 amino acids and having a molecular mass of 21.3 kDa .
The VEGF is fused to a 20 a.a His-tag at N-terminus and is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

VEGF His is supplied in 20mM Tris-HCl pH-8.5, 50% glycerol, 5mM DTT, 200mM NaCl & 2mM EDTA.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-PECAM1 Human/CYT-924

ProSpec-PECAM1 Human,ProSpec CYT-924,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,在 Sf9 杆状病毒中产生的 PECAM1 Human Recombinant 是一个单一的、非糖基化的多肽链，含有 582 个氨基酸，分子量为 65.5kDa。PECAM1 在 C 端与 8 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

PECAM1 Human

Platelet Endothelial Cell Adhesion Molecule 1 Human Recombinant
CYT-924
ProSpec-PECAM1 Human/CYT-924 2µg
ProSpec-PECAM1 Human/CYT-924 10µg
ProSpec-PECAM1 Human/CYT-924 1mg

Catalogue number

CYT-924

Synonyms

Platelet endothelial cell adhesion molecule, PECAM1, CD31, CD31/EndoCAM, endoCAM, GPIIA’, PECA1, PECAM-1, Platelet Endothelial Cell Adhesion Molecule 1.

血小板内皮细胞粘附分子、PECAM1、CD31、CD31/EndoCAM、endoCAM、GPIIA’、PECA1、PECAM-1、血小板内皮细胞粘附分子 1。

Introduction

Platelet endothelial cell adhesion molecule induces susceptibility to atherosclerosis. PECAM1 averts phagocyte ingestion of closely apposed viable cells by transmitting detachment signals, and transforms function upon apoptosis, thus promoting tethering of dying cells to phagocytes. The encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces as a result causing the viable cell’s active repulsion from the phagocyte.

血小板内皮细胞粘附分子诱导动脉粥样硬化易感性。 PECAM1 通过传递分离信号来避免吞噬细胞摄入紧密并列的活细胞，并在细胞凋亡时转变功能，从而促进垂死细胞与吞噬细胞的结合。通过 PECAM1 在两个细胞表面上的同源性相互作用，活细胞与吞噬细胞相遇，结果导致活细胞主动排斥吞噬细胞。

Description

PECAM1 Human Recombinant produced in Sf9 Baculovirus is a single, non-glycosylated polypeptide chain containing 582 amino acids and having a molecular mass of 65.5kDa .
PECAM1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒中产生的 PECAM1 Human Recombinant 是一个单一的、非糖基化的多肽链，含有 582 个氨基酸，分子量为 65.5kDa。
PECAM1 在 C 端与 8 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

PECAM1 protein solution containing Phosphate Buffered Saline and 10% glycerol.

Stability

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

QENSFTINSV DMKSLPDWTV QNGKNLTLQC FADVSTTSHV KPQHQMLFYK DDVLFYNISS MKSTESYFIP EVRIYDSGTY KCTVIVNNKE KTTAEYQVLV EGVPSPRVTL DKKEAIQGGI VRVNCSVPEE KAPIHFTIEK LELNEKMVKL KREKNSRDQN FVILEFPVEE QDRVLSFRCQ ARIISGIHMQ TSESTKSELV TVTESFSTPK FHISPTGMIM EGAQLHIKCT IQVTHLAQEF PEIIIQKDKA IVAHNRHGNK AVYSVMAMVE HSGNYTCKVE SSRISKVSSI VVNITELFSK PELESSFTHL DQGERLNLSC SIPGAPPANF TIQKEDTIVS QTQDFTKIAS KGTYICT AGIDKVVKKS NTVQIVVCEM LSQPRISYDA QFEVIKGQTI EVRCESISGT LPISYQLLKT SKVLENSTKN SNDPAVFKDN PTEDVEYQCV ADNCHSHAKM LSEVLRVKVI APVDEVQISI LSSKVVESGE DIVLQCAVNE GSGPITYKFY REKEGKPFYQ MTSNATQAFW TKQKASKEQE GEYYCTAFNR ANHASSVPRS KILTVRVILA PWKKVEHHHH HH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF Human, Yeast

Description

Catalogue number

CYT-577

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types . VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Description

Vascular Endothelial Growth Factor Human Recombinant produced in Yeast is a double, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 42 kDa.

Source

Pichia Pastoris.

Physical Appearance

Sterile Filtered colorless liquid formulation.

Formulation

The protein contains 20mM Phosphate- Buffered Saline, pH 7.4.

Stability

Vascular Endothelial Growth Factor Human although stable at 15°C for 2 weeks, should be stored below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Biological Activity

Determined by its ability to stimulate 3H-Thymidine incorporation in human umbilical vein endothelial cells, the ED50 for this effect was found to be 2-6ng/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TAFA2 Human/CYT-118

ProSpec-TAFA2 Human,ProSpec CYT-118,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Family with sequence similarity 19 -like) member A2, Chemokine-like protein TAFA-2, protein FAM19A2.

TAFA2 Human

Family with Sequence Similarity 19 Member A2 Human Recombinant
CYT-118
ProSpec-TAFA2 Human/CYT-118 5µg
ProSpec-TAFA2 Human/CYT-118 20µg
ProSpec-TAFA2 Human/CYT-118 1mg

Catalogue number

CYT-118

Synonyms

Family with sequence similarity 19 -like) member A2, Chemokine-like protein TAFA-2, protein FAM19A2.

Introduction

TAFA-2 is a 11 kDa secreted protein that belongs to the FAM19/TAFA family of chemokine-like proteins. Similar to other FAM19/TAFA family members, mature TAFA-1 contains 10 regularly spaced cysteine residues with the same pattern: CX7CCX13CXCX14CX11CX4CX5CX10C . Human TAFA-2 is 97% aa identical to mouse TAFA-2 and is expressed in the central nervous system , colon, heart, lung, spleen, kidney, and thymus, however its expression in the CNS is 50 to 1000 fold higher than in other tissues. The biological roles of TAFA family members have not yet been determined.

TAFA-2 是一种 11 kDa 的分泌蛋白，属于趋化因子样蛋白的 FAM19/TAFA 家族。与其他 FAM19/TAFA 家族成员类似，成熟的 TAFA-1 包含 10 个具有相同模式的规则间隔的半胱氨酸残基：CX7CCX13CXCX14CX11CX4CX5CX10C。人 TAFA-2 与小鼠 TAFA-2 有 97% 的氨基酸相同，并在中枢神经系统、结肠、心脏、肺、脾、肾和胸腺中表达，但其在中枢神经系统中的表达比在中枢神经系统中高 50 至 1000 倍其他组织。 TAFA 家族成员的生物学作用尚未确定。

Description

TAFA2 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 101 amino acids and having a molecular mass of 11.2kDa.
The TAFA2 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TAFA2 Human Recombinant 是一种非糖基化的多肽链，含有 101 个氨基酸，分子量为 11.2kDa。
TAFA2 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH7.4.

Solubility

It is recommended to reconstitute the lyophilized TAFA2 in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的 TAFA2 溶解在不低于 100µg/ml 的无菌 18M-cm H2O 中，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TAFA2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TAFA2 should be stored at 4C between 2-7 days and for future use below -18C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TAFA2 虽然在室温下可稳定保存 3 周，但应在 -18C 以下干燥储存。重组后，TAFA2 应在 4C 下储存 2-7 天，以备将来在 -18C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

ANHHKAHHVK TGTCEVVALH RCCNKNKIEE RSQTVKCSCF PGQVAGTTRA APSCVDASIV EQKWWCHMQP CLEGEECKVL PDRKGWSCSS GNKVKTTRVT H

Biological Activity

Fully biologically active when compared to standard. Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-VEGF Mouse

Description

Catalogue number

CYT-336

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types . VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesisand cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Description

Vascular Endothelial Growth Factor Mouse Recombinant produced in E.Coli is a disulfide-linked homodimeric, double polypeptide chains containing 165 amino acids and having a molecular mass of 38.8kDa.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a 0.2µm filtered solution with PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Vascular Endothelial Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Biological Activity

The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells using a concentration range of 5.0 ng/ml corresponding to a specific activity of 200,000IU/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

References

ProSpec-Vaspin Human/CYT-1132

ProSpec-Vaspin Human,ProSpec CYT-1132,冻干的 Vaspin 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。复溶后，Vaspin 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

Vaspin Human

Vaspin Recombinant Human
CYT-1132
ProSpec-Vaspin Human/CYT-1132 5µg
ProSpec-Vaspin Human/CYT-1132 25µg
ProSpec-Vaspin Human/CYT-1132 1mg

Catalogue number

CYT-1132

Synonyms

Serpin A12 precursor, Visceral adipose-specific serpin, Visceral adipose tissue- derived serine protease inhibitor, Vaspin, OL-64, SERPINA12, Serine proteinase inhibitor, clade A, antitrypsin, alpha-1 antiproteinase.

Serpin A12 前体、内脏脂肪特异性 serpin、内脏脂肪组织来源的丝氨酸蛋白酶抑制剂、Vaspin、OL-64、SERPINA12、丝氨酸蛋白酶抑制剂、进化枝 A、抗胰蛋白酶、α-1 抗蛋白酶。

Introduction

Vaspin is a newly identified adipokine, which is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism.

Vaspin 是一种新发现的脂肪因子，是丝氨酸蛋白酶抑制剂家族的成员。 Vaspin 也是一种独特的胰岛素增敏脂肪细胞因子。最近的一份出版物表明，脂肪组织中人类 vaspin mRNA 表达的诱导以脂肪库特异性方式进行调节，并且可能与肥胖、胰岛素抵抗和葡萄糖代谢的参数有关。

Description

Vaspin Human Recombinant produced in E.Coli is a single, non- glycosylated polypeptide chain containing 394 amino acids and having a molecular mass of 45.1kDa.
Vaspin is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Vaspin Human Recombinant 是单条非糖基化多肽链，含有 394 个氨基酸，分子量为 45.1kDa。
Vaspin 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris-HCl, pH 8.0, 150mM NaCl and 0.02 % Tween-20.

从 20mM Tris-HCl、pH 8.0、150mM NaCl 和 0.02% Tween-20 中的 0.2μm 过滤浓缩溶液冻干。

Solubility

It is recommended to reconstitute the lyophilized Vaspin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 Vaspin，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Vaspin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vaspin should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

冻干的 Vaspin 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。复溶后，Vaspin 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
请防止冻融循环。

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

LKPSFSPRNY KALSEVQGWK QRMAAKELAR QNMDLGFKLL KKLAFYNPGR NIFLSPLSIS TAFSMLCLGA QDSTLDEIKQ GFNFRKMPEK DLHEGFHYII HELTQKTQDL KLSIGNTLFI DQRLQPQRKF LEDAKNFYSA ETILTNFQNL EMAQKQINDF ISQKTHGKIN NLIENIDPGT VMLLANYIFF RARWKHEFDP NVTKEEDFFL EKNSSVKVPM MFRSGIYQVG YDDKLSCTIL EIPYQKNITA IFILPDEGKL KHLEKGLQVD TFSRWKTLLS RRVVDVSVPR LHMTGTFDLK KTLSYIGVSK IFEEHGDLTK IAPHRSLKVG EAVHKAELKM DERGTEGAAG TGAQTLPMET PLVVKIDKPY LLLIYSEKIP SVLFLGKIVN PIGK.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-VEGF Mouse (121 a.a.), Yeast

Description

Catalogue number

CYT-1125

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Introduction

Vascular endothelial growth factor is an important signaling protein involved in both angiogenesis and vasculogenesis.VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types .VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Elevated levels of VEGF is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in VEGF have been associated with proliferative and nonproliferative diabetic retinopathy.

Description

Vascular Endothelial Growth Factor Mouse Recombinant produced in yeast is a disulfide-linked homodimer consisting of 2×121 amino acid polypeptide chains, having a molecular mass of approximately 20.7kDa each.
VEGF is purified by proprietary chromatographic techniques.

Source

Saccharomyces cerevisiae

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vascular Endothelial Growth Factor should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R.

Biological Activity

The activity was measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells and was found to be 1-4 ng/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-Vaspin Human, His/CYT-459

ProSpec-Vaspin Human His,ProSpec CYT-459,在大肠杆菌中产生的 Vaspin Human Recombinant 是一种单一的、非糖基化的 His 标签多肽链，含有 415 个氨基酸，分子量为 47 kDa。
Vaspin 通过专有的色谱技术进行纯化。

Vaspin Human, His

Vaspin Human Recombinant, His Tag
CYT-459
ProSpec-Vaspin Human, His/CYT-459 5µg
ProSpec-Vaspin Human, His/CYT-459 25µg
ProSpec-Vaspin Human, His/CYT-459 1mg

Catalogue number

CYT-459

Synonyms

Introduction

Vaspin , a newly identified adipokine, which is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism.

Description

Vaspin Human Recombinant produced in E.Coli is a single, non-glycosylated, His Tag, polypeptide chain containing 415 amino acids and having a molecular mass of 47 kDa.
The Vaspin is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Vaspin Human Recombinant 是一种单一的、非糖基化的 His 标签多肽链，含有 415 个氨基酸，分子量为 47 kDa。
Vaspin 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

Vaspin in 20mM Tris pH-8, 0.2mM PMSF and 10% glycerol.

Stability

Liquid Vaspin although stable at 10°C for 1 week, should be stored below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

液体 Vaspin 虽然在 10°C 下稳定 1 周，但应储存在 -18°C 以下。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MLKPSFSPRN YKALSEVQGW KQRMAAKELA RQNMDLGFKL LKKLAFYNPG RNIFLSPLSI STAFSMLCLG AQDSTLDEIK QGFNFRKMPE KDLHEGFHYI IHELTQKTQD LKLSIGNTLF IDQRLQPQRK FLEDAKNFYS AETILTNFQN LEMAQKQIND FISQKTHGKI NNLIENIDPG TVMLLANYIF FRARWKHEFD PNVTKEEDFF LEKNSSVKVP MMFRSGIYQV GYDDKLSCTI LEIPYQKNIT AIFILPDEGK LKHLEKGLQV DTFSRWKTLL SRRVVDVSVP RLHMTGTFDL KKTLSYIGVS KIFEEHGDLT KIAPHRSLKV GEAVHKAELK MDERGTEGAA GTGAQTLPME TPLVVKIDKP YLLLIYSEKI PSVLFLGKIV NPIGK.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

TGFB2 Human, HEK

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Introduction

Description

Source

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Formulation

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Purity

Biological Activity

Usage

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