ProSpec-sRANKL Human, GST

Description

Catalogue number

CYT-631

Synonyms

Soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf, hRANKL2.

Introduction

RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. By injecting soluble RANKL, novel osteopenia model mice were established in only 50 hours. Degree of bone loss can be controlled by changing doses of sRANKL. RANKL can be used in establishing osteopenia model for in vivo screening of drugs for osteoporosis, determination of effect and mechanism, evaluation of bone anabolic drugs.

Description

RANKL Human Recombinant fused to GST tag produced in E.Coli is a single, non-glycosylated polypeptide having a molecular mass of 47 kDa.
RANKL is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorelss clear solution.

Formulation

The soluble RANKL protein solution contains 1mM EDTA and PBS pH-7.4.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF14 Human/CYT-821

ProSpec-TNFRSF14 Human,ProSpec CYT-821,TNFRSF14 是 TNF 受体超家族的成员，是一种 I 型跨膜蛋白。 TNFRSF14 在外周血 T 细胞、B 细胞、单核细胞和富含淋巴细胞的各种组织中表达。 TNFRSF14 作为激活淋巴样细胞的共刺激因子和对疱疹病毒感染的威慑物起作用。此外，TNFRSF14 促进 T 细胞的增殖，并引发各种肿瘤细胞的凋亡。

TNFRSF14 Human

HVEM-Fc Human Recombinant
CYT-821
ProSpec-TNFRSF14 Human/CYT-821 20µg
ProSpec-TNFRSF14 Human/CYT-821 100µg
ProSpec-TNFRSF14 Human/CYT-821 1mg

Catalogue number

CYT-821

Synonyms

Tumor Necrosis Factor Receptor Superfamily Member 14, HVEM, TR2, Herpes Virus Entry Mediator A, Tumor Necrosis Factor Receptor-Like 2, Herpesvirus Entry Mediator, HVEA, ATAR, CD270, LIGHTR, CD40-Like Protein, Tumor Necrosis Factor Receptor-Like Gene2.

Introduction

TNFRSF14, a member of the TNF receptor superfamily, is a type I transmembrane protein. TNFRSF14 is expressed in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. TNFRSF14 operates as a co-stimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. Additionally, TNFRSF14 encourages the proliferation of T cells, and triggers apoptosis of various tumor cells.

TNFRSF14 是 TNF 受体超家族的成员，是一种 I 型跨膜蛋白。 TNFRSF14 在外周血 T 细胞、B 细胞、单核细胞和富含淋巴细胞的各种组织中表达。 TNFRSF14 作为激活淋巴样细胞的共刺激因子和对疱疹病毒感染的威慑物起作用。此外，TNFRSF14 促进 T 细胞的增殖，并引发各种肿瘤细胞的凋亡。

Description

TNFRSF14 Human Recombinant produced in Pichia Pastoris is a single, glycosylated, polypeptide chain containing 396 amino acids and having a molecular mass of 58.0kDa.

However, TNFRSF14 migrates with an apparent molecular mass of 70 kDa in -PAGE under reducing conditions.

Pichia Pastoris 生产的 TNFRSF14 人重组体是单条糖基化多肽链，包含 396 个氨基酸，分子量为 58.0kDa。
然而，在还原条件下，TNFRSF14 在 -PAGE 中以 70 kDa 的表观分子量迁移。

Source

Pichia Pastoris.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

TNFRSF14 protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4, with 3 % Trehalose.

Solubility

It is recommended to reconstitute the lyophilized TNFRSF14 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 TNFRSF14，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNFRSF14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFRSF14 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .

Please prevent freeze-thaw cycles.

冻干的 TNFRSF14 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。重组后，TNFRSF14 应在 4°C 下储存 2-7 天，以备将来在低于 -18°C 的温度下使用。对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:

Analysis by RP-HPLC.

Analysis by -PAGE.

Amino acid sequence

LPSCKEDEYP VGSECCPKCS PGYRVKEACG ELTGTVCEPC PPGTYIAHLN GLSKCLQCQM CDPAMGLRAS RNCSRTENAV CGCSPGHFCI VQDGDHCAAC RAYATSSPGQ RVQKGGTESQ DTLCQNCPPG TFSPNGTLEE CQHQTKCSWL VTKAGAGTSS SHWVEPKSSD KTHTCPPCPA PEFEGAPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPTP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK

Biological Activity

Fully biologically active when compared to standard. The biologically active as determined by its ability to inhibit TNF-beta -mediated cytotoxicity using murine L929 cells.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF14 Human, His/CYT-800

ProSpec-TNFRSF14 Human His,ProSpec CYT-800,TNFRSF14 是 TNF 受体超家族的成员，是一种 I 型跨膜蛋白。 TNFRSF14 在外周血 T 细胞、B 细胞、单核细胞和富含淋巴细胞的各种组织中表达。 TNFRSF14 作为激活淋巴样细胞的共刺激因子和对疱疹病毒感染的威慑物起作用。此外，TNFRSF14 促进 T 细胞的增殖，并引发各种肿瘤细胞的凋亡。

TNFRSF14 Human, His

HVEM Human Recombinant, His Tag
CYT-800
ProSpec-TNFRSF14 Human, His/CYT-800 5µg
ProSpec-TNFRSF14 Human, His/CYT-800 25µg
ProSpec-TNFRSF14 Human, His/CYT-800 1mg

Catalogue number

CYT-800

Synonyms

Introduction

Description

TNFRSF14 Human Recombinant produced in E. Coli is a single, glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 19.7kDa.

TNFRSF14 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFRSF14 Human Recombinant 是一条单一的糖基化多肽链，包含 187 个氨基酸，分子量为 19.7kDa。
TNFRSF14 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

E.coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

The TNFRSF14 solution containing 20mM Tris-HCl , 0.1M NaCl and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.

Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 85% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSLPSCKED EYPVGSECCP KCSPGYRVKE ACGELTGTVC EPCPPGTYIA HLNGLSKCLQ CQMCDPAMGL RASRNCSRTE NAVCGCSPGH FCIVQDGDHC AACRAYATSS PGQRVQKGGT ESQDTLCQNC PPGTFSPNGT LEECQHQTKC SWLVTKAGAG TSSSHWV.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-sRANKL Human, His

Description

Catalogue number

CYT-692

Synonyms

Introduction

Description

sRANKL Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 22.3kDa.
sRANKL is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

sRANKL protein solution containing 20mM Tris-HCl buffer , 20% glycerol, 0.1M NaCl and 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 80.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MIRAEKAMVD GSWLDLAKRS KLEAQPFAHL TINATDIPSG SHKVSLSSWY HDRGWAKISN MTFSNGKLIV NQDGFYYLYA NICFRHHETS GDLATEYLQL MVYVTKTSIK IPSSHTLMKG GSTKYWSGNS EFHFYSINVG GFFKLRSGEE ISIEVSNPSL LDPDQDATYF GAFKVRDID.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF14 Human, Sf9/CYT-981

ProSpec-TNFRSF14 Human Sf9,ProSpec CYT-981,Tumor necrosis factor receptor superfamily member 14 isoform 1, TNFRSF14, ATAR, CD270, HVEA, HVEM, LIGHTR, TR2, HVEM-Fc, Sf9, Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, Herpesvirus entry mediator A, HveA.

TNFRSF14 Human, Sf9

HVEM-Fc Human Recombinant, Sf9
CYT-981
ProSpec-TNFRSF14 Human, Sf9/CYT-981 1µg
ProSpec-TNFRSF14 Human, Sf9/CYT-981 5µg
ProSpec-TNFRSF14 Human, Sf9/CYT-981 50µg

Catalogue number

CYT-981

Synonyms

Tumor necrosis factor receptor superfamily member 14 isoform 1, TNFRSF14, ATAR, CD270, HVEA, HVEM, LIGHTR, TR2, HVEM-Fc, Sf9, Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, Herpesvirus entry mediator A, HveA.

Introduction

Description

TNFRSF14 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 406 amino acids and having a molecular mass of 46.6kDa .
TNFRSF14 is fused to a 239 amino acid IgG His-Tag at C-terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFRSF14 Human Recombinant 是单条糖基化多肽链，包含 406 个氨基酸，分子量为 46.6kDa。
TNFRSF14 在 C 端与 239 个氨基酸的 IgG His-Tag 融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNFRSF14 protein solution containing Phosphate Buffered Saline and 10% glycerol.

Stability

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by analysis by -PAGE.

Amino acid sequence

ADPLPSCKED EYPVGSECCP KCSPGYRVKE ACGELTGTVC EPCPPGTYIA HLNGLSKCLQ CQMCDPAMGL RASRNCSRTE NAVCGCSPGH FCIVQDGDHC AACRAYATSS PGQRVQKGGT ESQDTLCQNC PPGTFSPNGT LEECQHQTKC SWLVTKAGAG TSSSHWVLEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-sRANKL Mouse

Description

Catalogue number

CYT-320

Synonyms

Introduction

RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Description

sRANKL Mouse Recombinant produced in E.coli is single, non-glycosylated, polypeptide chain containing 174 amino acids and having a total molecular mass of 19.9kDa. CD254 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized with 10mM Na₂PO₄, pH 7.5 & 50mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein . Please prevent freeze-thaw cycles.

Amino acid sequence

PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID.

Purity

Greater than 95.0% as determined by -PAGE.

Biological Activity

Measured by its ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 50ng/ml shown in “Corning® Osteo Assay Surface 24 Well Plates with Transwell® Permeable Supports- A Useful Tool for Co-Culture Studies” by Rebecca M. Wood and Mark Rothenber, corresponding to a specific activity of 20,000Units/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF14 Mouse/CYT-1145

ProSpec-TNFRSF14 Mouse,ProSpec CYT-1145,疱疹病毒进入介质或 HVEM 或肿瘤坏死因子受体超家族成员 14 或 TNFRSF14，是位于细胞表面的受体 TNF 受体家族的一部分。该受体的细胞质区域可以结合各种TNF受体相关因子蛋白。这些蛋白质可以介导激活免疫反应的途径。 MITF 正在调节 TNFRSF14 基因的表达。

TNFRSF14 Mouse

HVEM Mouse Recombinant
CYT-1145
ProSpec-TNFRSF14 Mouse/CYT-1145 2µg
ProSpec-TNFRSF14 Mouse/CYT-1145 10µg
ProSpec-TNFRSF14 Mouse/CYT-1145 100µg

Catalogue number

CYT-1145

Synonyms

Introduction

Herpesvirus entry mediator or HVEM or tumour necrosis factor receptor superfamily member 14 or TNFRSF14, is part of the TNF receptors family that is a receptor located on the cell surface. The cytoplasmic area of this receptor can bind all sorts of TNF receptor associated factor protein. Those proteins can mediate pathways activating an immune response. The MITF is regulating TNFRSF14 gene expression.

疱疹病毒进入介质或 HVEM 或肿瘤坏死因子受体超家族成员 14 或 TNFRSF14，是位于细胞表面的受体 TNF 受体家族的一部分。该受体的细胞质区域可以结合各种TNF受体相关因子蛋白。这些蛋白质可以介导激活免疫反应的途径。 MITF 正在调节 TNFRSF14 基因的表达。

Description

TNFRSF14 Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 407 amino acids and having a molecular mass of 45.3kDa.
TNFRSF14 is fused to a 239 amino acid hIgG-His-Tag at C-terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFRSF14 小鼠是单条糖基化多肽链，包含 407 个氨基酸，分子量为 45.3kDa。
TNFRSF14 在 C 末端与 239 个氨基酸的 hIgG-His-Tag 融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The TNFRSF14 solution contains 10% glycerol and Phosphate-Buffered Saline .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

QPSCRQEEFL VGDECCPMCN PGYHVKQVCS EHTGTVCAPC PPQTYTAHAN GLSKCLPCGV
CDPDMGLLTW QECSSWKDTV CRCIPGYFCE NQDGSHCSTC LQHTTCPPGQ RVEKRGTHDQ
DTVCADCLTG TFSLGGTQEE CLPWTNCSAF QQEVRRGTNS TDTTCSSQLE PKSCDKTHTC
PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN
AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP
QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL
YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-RBP Human, Native

Description

Catalogue number

CYT-1203

Introduction

Human Retinol Binding Protein, also known as RBP, is responsible for transporting and binding vitamin A. Human RBP has a binding site for 1 molecule of retinol and circulates in the plasma together with prealbumin as a protein complex. The prealbumin binding prevents greater glomerular losses of the human RBP. Only the retinol-free form of the RBP, which has no affinity for prealbumin, undergoes glomerular filtration unhindered as a result of its low Mw. Human RBP is re-absorbed by the tubular cells and catabolized there.

Description

Human Retinol Binding Protein Native produced in urine from the patients with renal tubular proteinuria having a molecular mass of approximately 21kD.

Source

Urine from the patients with renal tubular proteinuria.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from 20mM NH₄HCO₃.

Solubility

It is recommended to reconstitute the lyophilized RBP Human in phosphate buffer containing 0.15M NaCl.

Stability

RBP Human although stable at room temperature for 3 weeks, should be stored between 2-8°C.

Purity

Greater than 96.0%.

Human Virus Test

Starting material donor has been tested and certified negative for antibodies to HIV-1, HIV-2, HCV, HBSAG and Syphilis.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-RBP1 Human

Description

Catalogue number

CYT-122

Synonyms

Retinol binding protein 1 cellular, CRBP, CRBP1, CRABP-I, RBPC.

Introduction

RBP1 is a member of the calycin superfamily and fatty-acid binding protein family. RBP1 is the carrier protein which takes part in the transport of retinol from the liver storage site to peripheral tissue. Additionally, RBP1 performs as a bridging molecule to recruit histone deacetylases which is a forceful regulator of gene expression. RBP1 is found in almost all the tissues with higher expression in pancreas, adrenal gland and pituitary gland, fetal liver and adult ovary.

Description

RBP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 220 amino acids and having a molecular mass of 24.7kDa.
RBP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

E.coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

RBP1 protein solution containing 20mM Tris-HCl buffer , 200mM NaCl, 2mM DTT and 20% glycerol.

Stability

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMDPPAGF VRAGNPAVAA PQSPLSPEGA HFRAAHHPRS TGSRCPGSLQ PSRPLVANWL QSLPEMPVDF TGYWKMLVNE NFEEYLRALD VNVALRKIAN LLKPDKEIVQ DGDHMIIRTL STFRNYIMDF QVGKEFEEDL TGIDDRKCMT TVSWDGDKLQ CVQKGEKEGR GWTQWIEGDE LHLEMRVEGV VCKQVFKKVQ

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF17 Human/CYT-598

ProSpec-TNFRSF17 Human,ProSpec CYT-598,TNFRSF17 是 tnfsf13b/blys/baff 和 tnfsf13/april 的受体。 TNFRSF17 促进 b 细胞存活并在调节体液免疫中发挥作用。 TNFRSF17 激活 nf-kappa-b 和 jnk。 TNFRSF17 是 TNF 受体超家族的成员。

TNFRSF17 Human

B-Cell Maturation Antigen Human Recombinant
CYT-598
ProSpec-TNFRSF17 Human/CYT-598 5µg
ProSpec-TNFRSF17 Human/CYT-598 20µg
ProSpec-TNFRSF17 Human/CYT-598 1mg

Catalogue number

CYT-598

Synonyms

BCMA, CD269, Tumor Necrosis Factor Receptor Superfamily Member 17, BCM, TNFRSF17, B-cell maturation protein, CD269 antigen.

Introduction

TNFRSF17 is a receptor for tnfsf13b/blys/baff and tnfsf13/april. TNFRSF17 promotes b-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 activates nf-kappa-b and jnk. TNFRSF17 is a member of the TNF-receptor superfamily. TNFRSF17 is expressed in mature B lymphocytes, and is invloved in B cell development and autoimmune response. TNFRSF17 specifically binds to the tumor TNFSF13B/TALL-1/BAFF, which causes NF-kappaB and MAPK8/JNK activation. TNFRSF17 binds to a variety of TRAF family members, and therefore transduces signals for cell survival and proliferation. TNFRSF17 is a type III membrane protein having 1 extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs. TNFRSF17 appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells.

TNFRSF17 是 tnfsf13b/blys/baff 和 tnfsf13/april 的受体。 TNFRSF17 促进 b 细胞存活并在调节体液免疫中发挥作用。 TNFRSF17 激活 nf-kappa-b 和 jnk。 TNFRSF17 是 TNF 受体超家族的成员。 TNFRSF17 在成熟 B 淋巴细胞中表达，并参与 B 细胞发育和自身免疫反应。 TNFRSF17 特异性结合肿瘤 TNFSF13B/TALL-1/BAFF，导致 NF-kappaB 和 MAPK8/JNK 激活。 TNFRSF17 与多种 TRAF 家族成员结合，因此可转导细胞存活和增殖的信号。 TNFRSF17 是一种 III 型膜蛋白，具有 1 个胞外富含半胱氨酸的结构域。在 TNFRSF 中，它与 TACI 具有最高的同源性。 BCMA 和 TACI 均已显示与 TNF 配体超家族成员 APRIL 和 BAFF 结合。已在免疫器官中发现 BCMA 表达。 TNFRSF17 似乎定位于高尔基体隔室。已显示 BCMA 与 APRIL 或 BAFF 的结合可刺激外周血 B 细胞中 IgM 的产生并增加培养的 B 细胞的存活率。

Description

TNFRSF17 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 5.3 kDa.
The TNFRSF17 is purified by standard chromatographic techniques.

在大肠杆菌中产生的 TNFRSF17 Human Recombinant 是一种单一的、非糖基化的多肽链，含有 50 个氨基酸，分子量为 5.3 kDa。
TNFRSF17 通过标准色谱技术纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

1mg of TNFRSF17 Human contain 20mM sodium phosphate buffer, pH-7.4, and 130mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized TNFRSF17 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中重新配制冻干的 TNFRSF17，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNFRSF17 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFRSF17 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFRSF17 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。重组后 TNFRSF17 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

AGQCSQNEYF DSLLHACIPC QLRCSSNTPP LTCQRYCNAS VTNSVKGTNA.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF17 Human, His/CYT-190

ProSpec-TNFRSF17 Human, His,ProSpec CYT-190,TNFRSF17 是 tnfsf13b/blys/baff 和 tnfsf13/april 的受体。 TNFRSF17 促进 b 细胞存活并在调节体液免疫中发挥作用。 TNFRSF17 激活 nf-kappa-b 和 jnk。 TNFRSF17 是 TNF 受体超家族的成员。

TNFRSF17 Human, His

B-Cell Maturation Antigen Human Recombinant, His Tag
CYT-190
5µg
20µg
1mg

Catalogue number

CYT-190

Synonyms

BCMA, CD269, Tumor Necrosis Factor Receptor Superfamily Member 17, BCM, TNFRSF17, B-cell maturation protein, CD269 antigen.

Introduction

Description

TNFRSF17 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 14.1kDa.
TNFRSF17 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFRSF17 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 130 个氨基酸，分子量为 14.1kDa。
TNFRSF17 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless liquid.

Formulation

TNFRSF17 protein solution containing 20mM Tris-HCl buffer , 0.15M NaCl, 10% glycerol and 1mM DTT.

Stability

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSRKINSEP LKDEFKNTGS GLLGMANIDL EKSRTGDEII LPRGLEYTVE ECTCEDCIKS KPKVDSDHCF PLPAMEEGAT ILVTTKTNDY CKSLPAALSA TEIEKSISAR.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-RBP2 Human

Description

Catalogue number

CYT-751

Synonyms

CRABP-II, CRBP2, CRBPII, RBPC2, Retinol-binding protein 2, Cellular retinol-binding protein II, RBP2.

Introduction

Retinol Binding Protein-2 which is present in the small intestinal epithelium takes part in the uptake and intracellular metabolism of vitamin A. Vitamin A is a fat-soluble vitamin essential for growth, reproduction, differentiation of epithelial tissues, and vision. RBP2 moderates the supply of retinoic acid to the nuclei of endometrial cells throughout the menstrual cycle.

Description

RBP2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18kDa. RBP2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

E.coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

RBP2 protein solution contains 20mM Tris-HCl buffer , 0.1M NaCl and 10% glycerol.

Stability

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMTRDQN GTWEMESNEN FEGYMKALDI DFATRKIAVR LTQTKVIDQD GDNFKTKTTS TFRNYDVDFT VGVEFDEYTK SLDNRHVKAL VTWEGDVLVC VQKGEKENRG WKQWIEGDKL YLELTCGDQV CRQVFKKK.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF17 Human, Sf9/CYT-1148

ProSpec-TNFRSF17 Human Sf9,ProSpec CYT-1148,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,B 细胞成熟抗原或 TNFRSF17 或肿瘤坏死因子受体超家族成员 17，是 TNF 受体蛋白家族的一部分。这种蛋白质是 TNFSF13B/BLyS/BAFF、TNFSF13/APRIL 和促进 B 细胞存活受体。

TNFRSF17 Human, Sf9

B-Cell Maturation Antigen, Sf9 Human Recombinant
CYT-1148
ProSpec-TNFRSF17 Human, Sf9/CYT-1148 2µg
ProSpec-TNFRSF17 Human, Sf9/CYT-1148 10µg
ProSpec-TNFRSF17 Human, Sf9/CYT-1148 1mg

Catalogue number

CYT-1148

Synonyms

BCMA, CD269, Tumor Necrosis Factor Receptor Superfamily Member 17, BCM, TNFRSF17, B-cell maturation protein, CD269 antigen

Introduction

B-Cell Maturation Antigen or TNFRSF17 or tumor necrosis factor receptor superfamily member 17, is part of the TNF receptor protein family. This protein is a TNFSF13B/BLyS/BAFF, TNFSF13/APRIL & promotes B-cell survival receptor. TNFRSF17 has a crucial part in the humoral immunity regulation. This protein is responsible for the activation of NF-kappa-B & JNK.

B 细胞成熟抗原或 TNFRSF17 或肿瘤坏死因子受体超家族成员 17，是 TNF 受体蛋白家族的一部分。这种蛋白质是 TNFSF13B/BLyS/BAFF、TNFSF13/APRIL 和促进 B 细胞存活受体。 TNFRSF17 在体液免疫调节中起着至关重要的作用。这种蛋白质负责激活 NF-kappa-B 和 JNK。

Description

TNFRSF17 Human Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 296 amino acids and having a molecular mass of 33.1Da.
TNFRSF17 is fused to a 242 amino acid hIgG-His-Tag at C-terminus and purified by proprietary chromatographic techniques.

杆状病毒产生的 TNFRSF17 Human Recombinant 是单条糖基化多肽链，包含 296 个氨基酸，分子量为 33.1Da。
TNFRSF17 在 C 端与 242 个氨基酸的 hIgG-His-Tag 融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TNFRSF17 protein contains 10% glycerol and Phosphate-Buffered Saline .

Stability

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

ADPMLQMAGQ CSQNEYFDSL LHACIPCQLR CSSNTPPLTC QRYCNASVTN SVKGTNALEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-RBP3 Human

Description

Catalogue number

CYT-1175

Synonyms

RBP3, IRBP, Retinol-binding protein 3, Interphotoreceptor retinoid-binding protein, Interstitial retinol-binding protein.

Introduction

Retinol-binding proteins are a family of proteins with various functions. Retinol and retinoic acid play crucial roles in the modulation of gene expression and overall development of an embryo. However, deficit or excess of either one of these substances can cause early embryo mortality or developmental malformations. Regulation of transport and metabolism of retinol necessary for a successful pregnancy is accomplished via RBP. Retinol binding proteins have been identified within the uterus, embryo, and extraembryonic tissue of the bovine, ovine, and porcine, therefore RBP takes part in proper retinol exposure to the embryo and successful transport at the maternal-fetal interface.

Description

RBP3 Human Recombinant produced in E.Coli is a single, non-glycosylated Polypeptide chain. RBP3 is fused to a 6 a.a His tag at N-terminal and is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

RBP3 was lyophilized from a concentrated 0.2µm solution in PBS pH, 7.4.

Solubility

It is recommended to reconstitute the lyophilized RBP3 in sterile distilled H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized RBP3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution. Retinol Binding Protein-3 should be stored at 4°C between 2-7 days and for future use below -18°C.

Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-RBP4 Human

Description

Catalogue number

CYT-535

Synonyms

Retinol Binding Protein 4, RBP-4, RBP4, Plasma retinol-binding protein, PRBP, RBP.

Introduction

Retinol binding protein 4 belongs to the lipocalin family and is the specific carrier for retinol in the blood. This protein was found to be expressed and secreted by adipose tissue, and was strongly associated with insulin resistance. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli.
RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the rbp-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.

Description

RBP-4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 184 amino acids and having a molecular mass of 21 kDa.
The Retinol Binding Protein-4 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

1 mg/ml in PBS pH-7.4.

Stability

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MERDCRVSSF RVKENFDKAR FSGTWYAMAK KDPEGLFLQD NIVAEFSVDE TGQMSATAKGRVRLLNNWDV CADMVGTFTD TEDPAKFKMK YWGVASFLQK GNDDHWIVDT DYDTYAVQYS CRLLNLDGTC ADSYSFVFSR DPNGLPPEAQ KIVRQRQEEL CLARQYRLIV HNGYCDGRSE RNLL.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF21 Human/CYT-1073

ProSpec-TNFRSF21 Human,ProSpec CYT-1073,肿瘤坏死因子受体超家族成员 21 或 TNFRSF21，是一种位于 TNF 受体超家族细胞膜中的蛋白质。通过激活 NF-kappaB 通路，TNFRSF21 促进细胞凋亡。由激活 caspase 3 和 caspase 6 引起的细胞变性，TNFRSF21 与神经元细胞体和轴突中的 N 端 APP 结合，导致细胞凋亡。 TNFRSF21 参与由 T 细胞受体刺激激活的信号级联。

TNFRSF21 Human

TNF Ligand Receptor Superfamily Member 21 Human Recombinant
CYT-1073
ProSpec-TNFRSF21 Human/CYT-1073 2µg
ProSpec-TNFRSF21 Human/CYT-1073 10µg
ProSpec-TNFRSF21 Human/CYT-1073 1mg

Catalogue number

CYT-1073

Synonyms

Tumor necrosis factor receptor superfamily member 21, BM-018, CD358, DR6, Death receptor 6.

Introduction

Tumor necrosis factor receptor superfamily member 21 or TNFRSF21, is a protein that located in the cell membrane from the TNF receptor superfamily. By activating the NF-kappaB pathway, TNFRSF21 promotes cell apoptosis. The degeneration of cells caused by activating caspase 3 and caspase 6, the TNFRSF21 binds to the N-terminal APP in neuronal cell bodies and axons which leads to apoptosis. TNFRSF21 takes part in signaling cascades activated by stimulation of T-cell receptor.

肿瘤坏死因子受体超家族成员 21 或 TNFRSF21，是一种位于 TNF 受体超家族细胞膜中的蛋白质。通过激活 NF-kappaB 通路，TNFRSF21 促进细胞凋亡。由激活 caspase 3 和 caspase 6 引起的细胞变性，TNFRSF21 与神经元细胞体和轴突中的 N 端 APP 结合，导致细胞凋亡。 TNFRSF21 参与由 T 细胞受体刺激激活的信号级联。

Description

TNFRSF21 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 547 amino acids and having a molecular mass of 60.4kDa. .
TNFRSF21 is expressed with a 239 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFRSF21 是单条糖基化多肽链，含有 547 个氨基酸，分子量为 60.4kDa。 .
TNFRSF21 在 C 末端用 239 个氨基酸 His 标签表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

TNFRSF21 protein solution contains phosphate buffered saline ,10% glycerol and 0.1 mM PMSF.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.

Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。

储存，在 -20°C 下冷冻更长时间。

对于长期储存，建议添加载体蛋白。

Avoid multiple freeze-thaw cycles.

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

QPEQKASNLI GTYRHVDRAT GQVLTCDKCP AGTYVSEHCT NTSLRVCSSC PVGTFTRHEN GIEKCHDCSQ PCPWPMIEKL PCAALTDREC TCPPGMFQSN ATCAPHTVCP VGWGVRKKGT ETEDVRCKQC ARGTFSDVPS SVMKCKAYTD CLSQNLVVIK PGTKETDNVC GTLPSFSSST SPSPGTAIFP RPEHMETHEV PSSTYVPKGM NSTESNSSAS VRPKVLSSIQ EGTVPDNTSS ARGKEDVNKT LPNLQVVNHQ QGPHHRHILK LLPSMEATGG EKSSTPIKGP KRGHPRQNLH KHFDINEHLE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-RBP4 Human, His

Description

Catalogue number

CYT-555

Synonyms

Retinol Binding Protein 4 plasma, RBP-4, RBP4, Plasma retinol-binding protein, PRBP, RBP.

Introduction

Description

RBP-4 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 183 amino acids fragment corresponding to the mature Retinol Binding Protein, having a total molecular mass of 25.57kDa and fused with a 4.5kDa amino-terminal hexahistidine tag.
The RBP4 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

RBP4 protein solution is supplied in 10mM Tris, 50mM NaCl, 1mM EDTA and 50% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF25 Human/CYT-980

ProSpec-TNFRSF25 Human,ProSpec CYT-980,Tumor necrosis factor receptor superfamily member 25, TNFRSF25, TNF Ligand Receptor Superfamily Member 25, APO-3, DDR3, DR3, LARD, TNFRSF12, TR3, TRAMP, WSL-1, WSL-LR, Apo-3, Apoptosis-inducing receptor AIR, Protein WSL, Apoptosis-mediating receptor DR3, Apoptosis-mediating receptor TRAMP, Death receptor 3, Lymphocyte-associated receptor of death.

TNFRSF25 Human

TNF Ligand Receptor Superfamily Member 25 Recombinant Human
CYT-980
ProSpec-TNFRSF25 Human/CYT-980 2µg
ProSpec-TNFRSF25 Human/CYT-980 10µg
ProSpec-TNFRSF25 Human/CYT-980 1mg

Catalogue number

CYT-980

Synonyms

Tumor necrosis factor receptor superfamily member 25, TNFRSF25, TNF Ligand Receptor Superfamily Member 25, APO-3, DDR3, DR3, LARD, TNFRSF12, TR3, TRAMP, WSL-1, WSL-LR, Apo-3, Apoptosis-inducing receptor AIR, Protein WSL, Apoptosis-mediating receptor DR3, Apoptosis-mediating receptor TRAMP, Death receptor 3, Lymphocyte-associated receptor of death.

Introduction

TNF Ligand Receptor Superfamily Member 25 belongs to the TNF receptor superfamily that binds to the TNF-like protein TL1A. TNFRSF25 interacts directly with the adapter TRADD and regulates lymphocyte homeostasis. TNFRSF25 is also mediates activation of NF-kappa-B and induces apoptosis. TNFRSF25 signals are vital to exert T helper cell 2 effector activity in Th2-polarized CD4 cells and co-stimulate interleukin-13 production by glycosphingolipid-activated NKT cells.

TNF 配体受体超家族成员 25 属于与 TNF 样蛋白 TL1A 结合的 TNF 受体超家族。 TNFRSF25 直接与接头 TRADD 相互作用并调节淋巴细胞稳态。 TNFRSF25 还介导 NF-kappa-B 的激活并诱导细胞凋亡。 TNFRSF25 信号对于在 Th2 极化 CD4 细胞中发挥 T 辅助细胞 2 效应子活性和共同刺激鞘糖脂激活的 NKT 细胞产生白细胞介素 13 至关重要。

Description

TNFRSF25 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 417 amino acids and having a molecular mass of 46.1kDa . TNFRSF25 is fused to a 242 amino acid IgG His-Tag at C-terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFRSF25 Human Recombinant 是单条糖基化多肽链，含有 417 个氨基酸，分子量为 46.1kDa。 TNFRSF25 在 C 末端与 242 个氨基酸的 IgG His-Tag 融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNFRSF25 protein solution containing Phosphate Buffered Saline and 10% glycerol.

Stability

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95.0% as determined by analysis by -PAGE.

Biological Activity

Determined by the binding ability in a functional ELISA with Human VEGI . The ED50 range ≤ 5ug/ml.

Amino acid sequence

ADPQGGTRSP RCDCAGDFHK KIGLFCCRGC PAGHYLKAPC TEPCGNSTCL VCPQDTFLAW ENHHNSECAR CQACDEQASQ VALENCSAVA DTRCGCKPGW FVECQVSQCV SSSPFYCQPC LDCGALHRHT RLLCSRRDTD CGTCLPGFYE HGDGCVSCPT STLGSCPERC AAVCGWRQLE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-RBP4 Mouse

Description

Catalogue number

CYT-1158

Synonyms

Retinol Binding Protein 4, RBP-4, RBP4, Plasma retinol-binding protein, PRBP, RBP.

Introduction

Retinol Binding Protein-4 or RBP4, is the retinol currier protein in the plasma. RBP4 takes part on the conversion of retinol to the stable and soluble retinol complex in the plasma. RBP4 carries retinol from the liver tissue where its store to peripheral tissues. RBP-retinol complex has interactions with transthyretin in the plasma, thus prevents its loss by filtration in the kidney.

Description

RBP4 Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 22.4kDa. RBP4 is fused to an 8 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The RBP4 solution contains 10% Glycerol and Phosphate-Buffered Saline .

Stability

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

ERDCRVSSFR VKENFDKARF SGLWYAIAKK DPEGLFLQDN IIAEFSVDEK GHMSATAKGR VRLLSNWEVC ADMVGTFTDT EDPAKFKMKY WGVASFLQRG NDDHWIIDTD YDTFALQYSC RLQNLDGTCA DSYSFVFSRD PNGLSPETRR LVRQRQEELC LERQYRWIEH NGYCQSRPSR NSLLEHHHHH H.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF4 Human/CYT-1039

ProSpec-TNFRSF4 Human,ProSpec CYT-1039,TNFRSF4, TNF Receptor Superfamily Member 4, TAX Transcriptionally-Activated Glycoprotein 1 Receptor, OX40L Receptor, ACT35 Antigen, CD134 Antigen, TXGP1L, Tax-Transcriptionally Activated Glycoprotein 1 Receptor, Tumor Necrosis Factor Receptor Superfamily, Member 4, Tumor Necrosis Factor Receptor Superfamily Member 4 , Lymphoid Activation Antigene ACT35, OX40 Cell Surface Antigen, OX40 Homologue , ATC35 Antigen, OX40 Antigen , ACT35, CD134, IMD16, OX40.

TNFRSF4 Human

TNF Receptor Superfamily Member 4 Human Recombinant
CYT-1039
ProSpec-TNFRSF4 Human/CYT-1039 2µg
ProSpec-TNFRSF4 Human/CYT-1039 10µg
ProSpec-TNFRSF4 Human/CYT-1039 1mg

Catalogue number

CYT-1039

Synonyms

TNFRSF4, TNF Receptor Superfamily Member 4, TAX Transcriptionally-Activated Glycoprotein 1 Receptor, OX40L Receptor, ACT35 Antigen, CD134 Antigen, TXGP1L, Tax-Transcriptionally Activated Glycoprotein 1 Receptor, Tumor Necrosis Factor Receptor Superfamily, Member 4, Tumor Necrosis Factor Receptor Superfamily Member 4 , Lymphoid Activation Antigene ACT35, OX40 Cell Surface Antigen, OX40 Homologue , ATC35 Antigen, OX40 Antigen , ACT35, CD134, IMD16, OX40.

Introduction

TNFRSF4, also known as TNF Receptor Superfamily Member 4, is a T cell co-stimulatory molecule which belongs to the TNF receptor superfamily. TNFRSF4 coordinates with other co-stimulatory substances such as CD28, CD40, CD30, CD27 and 4-1BB to control the activation of the immune response. TNFRSF4 takes a vital part in antigen-specific T cell expansion as well as survival. TNFRSF4 is up-regulated on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7. In addition, TNFRSF4 regulates cytokine production from T cells, antigen presenting cells, natural killer cells and natural killer cells. TNFRSF4 regulates cytokine receptor signaling.

TNFRSF4，也称为TNF受体超家族成员4，是属于TNF受体超家族的T细胞共刺激分子。 TNFRSF4 与其他共刺激物质如 CD28、CD40、CD30、CD27 和 4-1BB 协调以控制免疫反应的激活。 TNFRSF4 在抗原特异性 T 细胞扩增和存活中起着至关重要的作用。在抗原呈递细胞与 TCR 结合以及 CD40-CD40 配体和 CD28-B7 共同刺激后，TNFRSF4 在 CD4+ 和 CD8+ T 细胞上上调。此外，TNFRSF4 调节 T 细胞、抗原呈递细胞、自然杀伤细胞和自然杀伤细胞的细胞因子产生。 TNFRSF4 调节细胞因子受体信号。

Description

TNFRSF4 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 425 amino acids and having a molecular mass of 46.9Da. . TNFRSF4 is expressed with a 239 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFRSF4 是单条糖基化多肽链，含有 425 个氨基酸，分子量为 46.9Da。 . TNFRSF4 在 C 末端以 239 个氨基酸 His 标签表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TNFRSF4 protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。

对于长期储存，建议添加载体蛋白。

避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Biological Activity

Measured by its binding ability in a functional ELISA with mouse OX40 Ligand/TNFSF4 The ED50 range ≤ 0.15 ug/ml.

Amino acid sequence

LHCVGDTYPS NDRCCHECRP GNGMVSRCSR SQNTVCRPCG PGFYNDVVSS KPCKPCTWCN LRSGSERKQL CTATQDTVCR CRAGTQPLDS YKPGVDCAPC PPGHFSPGDN QACKPWTNCT LAGKHTLQPA SNSSDAICED RDPPATQPQE TQGPPARPIT VQPTEAWPRT SQGPSTRPVE VPGGRALEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-RBP5 Human

Description

Catalogue number

CYT-650

Synonyms

Retinol-binding protein 5, Cellular retinol-binding protein III, CRBP-III, HRBPiso, RBP5, CRBP3, CRBPIII.

Introduction

RBP5 is a member of the Retinol-binding proteins family. Estimation of retinol-binding protein is used to determine visceral protein mass in nutritional studies related to health. RBP5 has a higher expression in the adult kidney and liver and to a lesser extent in the adult and fetal spleen, adult lymph nodes and appendix, and fetal liver and kidney. RBP5 expression is strongly decreased in hepatocellular carcinoma tissues.

Description

RBP5 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 18.1kDa. The RBP5 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The RBP5 solution contains 20mM Tris-HCl buffer , 10% glycerol, 2mM DTT and 100mM NaCl.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MPPNLTGYYR FVSQKNMEDY LQALNISLAV RKIALLLKPD KEIEHQGNHM TVRTLSTFRN YTVQFDVGVE FEEDLRSVDG RKCQTIVTWE EEHLVCVQKG EVPNRGWRHW LEGEMLYLEL TARDAVCEQV FRKVR.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF4 Mouse/CYT-1179

ProSpec-TNFRSF4 Mouse,ProSpec CYT-1179,Tumor necrosis factor receptor superfamily member 4, OX40 antigen, OX40L receptor, Txgp1, Tnfrsf4, tax-transcriptionally activated glycoprotein 1 receptor, TXGP1L, ACT35, Txgp, Ly-70, ACT3, OX4, CD134.

TNFRSF4 Mouse

TNF Receptor Superfamily Member 4 Mouse Recombinant
CYT-1179
ProSpec-TNFRSF4 Mouse/CYT-1179 2µg
ProSpec-TNFRSF4 Mouse/CYT-1179 10µg
ProSpec-TNFRSF4 Mouse/CYT-1179 1mg

Catalogue number

CYT-1179

Synonyms

Tumor necrosis factor receptor superfamily member 4, OX40 antigen, OX40L receptor, Txgp1, Tnfrsf4, tax-transcriptionally activated glycoprotein 1 receptor, TXGP1L, ACT35, Txgp, Ly-70, ACT3, OX4, CD134.

Introduction

Description

TNFRSF4 Mouse Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain containing 435 amino acids and having a molecular mass of 48.6kDa.
TNFRSF4 is fused to a 243 amino acid hIgG-His tag at C-terminus and purified by proprietary chromatographic techniques.

在 HEK293 细胞中产生的 TNFRSF4 Mouse Recombinant 是一条糖基化多肽链，包含 435 个氨基酸，分子量为 48.6kDa。
TNFRSF4 在 C 末端与 243 个氨基酸的 hIgG-His 标签融合，并通过专有色谱技术进行纯化。

Source

HEK293 cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TNFRSF4 protein contains Phosphate-Buffered Saline and 10% glycerol.

Stability

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

DGSMVTARRL NCVKHTYPSG HKCCRECQPG HGMVSRCDHT RDTLCHPCET GFYNEAVNYD TCKQCTQCNH RSGSELKQNC TPTQDTVCRC RPGTQPRQDS GYKLGVDCVP CPPGHFSPGN NQACKPWTNC TLSGKQTRHP ALDAVCE DRSLLATLLW ETQRPTFRPT TVQSTTVWPR TSELPSPPTL VTPEGPLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-RBP7 Human

Description

Catalogue number

CYT-023

Synonyms

Retinoid-binding protein 7, Cellular retinoic acid-binding protein 4, CRABP4, CRBP4, Cellular retinoic acid-binding protein IV, CRABP-IV, RBP7, MGC70641.

Introduction

RBP7 is a member of a superfamily of small cytoplasmic proteins which interact with hydrophobic ligands. RBP7 is cytoplasmic protein which, like CRBP I and CRBP II, forms ?-barrel structures and participates in the intracellular transport of retinol. RBP7 is a newly identified cellular retinol carrier, which is expressed in the kidney, heart and transverse colon in humans.

Description

RBP7 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 154 amino acids and having a molecular mass of 17.6kDa. The RBP7 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The RBP7 solution contains 20mM Tris-HCl buffer , 0.2M NaCl, 2mM DTT and 20% glycerol.

Stability

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MPADLSGTWT LLSSDNFEGY MLALGIDFAT RKIAKLLKPQ KVIEQNGDSF TIHTNSSLRN YFVKFKVGEE FDEDNRGLDN RKCKSLVIWD NDRLTCIQKG EKKNRGWTHW IEGDKLHLEM FCEGQVCKQT FQRA.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF6B Human/CYT-799

ProSpec-TNFRSF6B Human,ProSpec CYT-799,TNFRSF6B 是肿瘤坏死因子受体超家族的成员。 TNFRSF6B 作为诱饵受体与死亡受体竞争配体结合。除了通过中和 TL1A 诱导血管生成外，TNFRSF6B 在抑制 FasL 和 LIGHT 介导的细胞死亡和 T 细胞活化方面具有调节作用。

TNFRSF6B Human

Tumor Necrosis Factor Receptor Superfamily, Member 6b Human Recombinant
CYT-799
ProSpec-TNFRSF6B Human/CYT-799 5µg
ProSpec-TNFRSF6B Human/CYT-799 20µg
ProSpec-TNFRSF6B Human/CYT-799 1mg

Catalogue number

CYT-799

Synonyms

Tumor Necrosis Factor Receptor Superfamily, Member 6b Decoy, Decoy Receptor For Fas Ligand, Decoy Receptor 3, DCR3, M68E, M68, TR6, DJ583P15.1.1, DcR3.

Introduction

TNFRSF6B is a member of the Tumor necrosis factor receptor superfamily. TNFRSF6B performs as a decoy receptor which races against death receptors for ligand binding. TNFRSF6B has a regulatory role in suppressing FasL- and LIGHT-mediated cell death and T cell activation in addition to inducing angiogenesis via neutralization of TL1A.

TNFRSF6B 是肿瘤坏死因子受体超家族的成员。 TNFRSF6B 作为诱饵受体与死亡受体竞争配体结合。除了通过中和 TL1A 诱导血管生成外，TNFRSF6B 在抑制 FasL 和 LIGHT 介导的细胞死亡和 T 细胞活化方面具有调节作用。

Description

TNFRSF6B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 294 amino acids and having a molecular mass of 32.1kDa.

TNFRSF6B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFRSF6B Human Recombinant 是一条单一的、非糖基化的多肽链，包含 294 个氨基酸，分子量为 32.1kDa。
TNFRSF6B 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNFRSF6B protein solution containing 20mM Tris-HCl buffer , 0.4M Urea and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Please avoid freeze thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
请避免冻融循环。

Purity

Greater than 85% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSVAETPTY PWRDAETGER LVCAQCPPGT FVQRPCRRDS PTTCGPCPPR HYTQFWNYLE RCRYCNVLCG EREEEARACH ATHNRACRCR TGFFAHAGFC LEHASCPPGA GVIAPGTPSQ NTQCQPCPPG TFSASSSSSE QCQPHRNCTA LGLALNVPGS SSHDTLCTSC TGFPLSTRVP GAEECERAVI DFVAFQDISI KRLQRLLQAL EAPEGWGPTP RAGRAALQLK LRRRLTELLG AQDGALLVRL LQALRVARMP GLERSVRERF LPVH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-SCF Canine

Description

Catalogue number

CYT-194

Synonyms

Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.

Introduction

Stem cell factor / KIT ligand is a cytokine which binds CD117. SCF is also known as “steel factor” or “c-kit ligand”. SCF exists in two forms, cell surface bound SCF and soluble SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E .

Description

Stem Cell Factor Canine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18.4kDa Dalton.
The SCF is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized SCF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 97.0% as determined by HPLC and -PAGE.

Amino acid sequence

KGICGKRVTD DVKDVTKLVA NLPKDYKIAL KYVPGMDVLP SHCWISVMVE QLSVSLTDLL DKFSNISEGL SNYSIIDKLV KIVDDLVECT EGYSFENVKK APKSPELRLF TPEEFFRIFN RSIDAFKDLE TVASKSSECV VSSTLSPDKD SRVSVTKPFM LPPVA

Biological Activity

Fully biologically active when compared to standard. The ED50 as calculated by the dose-dependent stimulation of the proliferation of human TF-1 cells is less than 2.0ng/ml, corresponding to a specific activity of 5,000,000IU/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF8 Human/CYT-927

ProSpec-TNFRSF8 Human,ProSpec CYT-927,肿瘤坏死因子受体超家族成员 8 是 TNFSF8/CD30L 的受体。 TNFRSF8 在调节活化淋巴母细胞的细胞生长和转化中发挥作用。此外，TNFRSF8 蛋白通过激活 NF-kappa-B 来调节基因表达。 TNFRSF8 是细胞凋亡的调节剂，根据细胞类型诱导细胞死亡或增殖。

TNFRSF8 Human

CD30 Ligand Receptor Human Recombinant
CYT-927
ProSpec-TNFRSF8 Human/CYT-927 2µg
ProSpec-TNFRSF8 Human/CYT-927 10µg
ProSpec-TNFRSF8 Human/CYT-927 1mg

Catalogue number

CYT-927

Synonyms

Tumor Necrosis Factor Receptor Superfamily, Member 8, Lymphocyte Activation Antigen CD30, CD30L Receptor, Ki-1 Antigen, D1S166E, CD30, Cytokine Receptor CD30, CD30 Antigen, Ki-1.

Introduction

Tumor necrosis factor receptor superfamily member 8 is a receptor for TNFSF8/CD30L. TNFRSF8 has a role in the regulation of cellular growth and transformation of activated lymphoblasts. In addition, the TNFRSF8 protein regulates gene expression via activation of NF-kappa-B. TNFRSF8 being a regulator of apoptosis, induces cell death or proliferation, depending on the cell type.

肿瘤坏死因子受体超家族成员 8 是 TNFSF8/CD30L 的受体。 TNFRSF8 在调节活化淋巴母细胞的细胞生长和转化中发挥作用。此外，TNFRSF8 蛋白通过激活 NF-kappa-B 来调节基因表达。 TNFRSF8 是细胞凋亡的调节剂，根据细胞类型诱导细胞死亡或增殖。

Description

TNFRSF8 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 370 amino acids and having a molecular mass of 39.5kDa .

TNFRSF8 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFRSF8 是单条糖基化多肽链，包含 370 个氨基酸，分子量为 39.5kDa。
TNFRSF8 在 C 末端带有 9 个氨基酸 His 标签，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TNFRSF8 protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。

对于长期储存，建议添加载体蛋白。

避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-SCF Human

Description

Catalogue number

CYT-255

Synonyms

Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.

Introduction

Description

Stem Cell Factor Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.
The SCF is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a concentrated solution in water containing 0.02% NaHCO₃.

Solubility

It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by:
Analysis by SEC-HPLC.
Analysis by -PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Gly-Ile-Cys.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 500,000 IU/mg.

Protein content

Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard.

References

1.Title:Direct binding of Cbl to Y568 and Y936 of the stem cell factor receptor/c-Kit is required for ligand-induced ubiquitination, internalization and degradation.
Publication:Biochemical Journal Immediate Publication. Published on 19 Jun 2006 as manuscript BJ20060464.
Link:http://www.biochemj.org/bj/imps_x/pdf/BJ20060464.pdf

2.Title:The D816V Mutation of c-Kit Circumvents a Requirement for Src Family Kinases in c-Kit Signal Transduction.
Publication: March 5, 2009, doi: 10.1074/jbc.M808058200 April 24, 2009 The Journal of Biological Chemistry, 284, 11039-11047.
Link:http://www.jbc.org/content/284/17/11039.full

3.Title:Structural Basis for c-KIT Inhibition by the Suppressor of Cytokine Signaling 6 Ubiquitin Ligase.
Publication:First Published on October 28, 2010, doi: 10.1074/jbc.M110.173526 January 7, 2011 The Journal of Biological Chemistry, 286, 480-490.
Link:http://www.jbc.org/content/286/1/480.full

4. Title: Gab2 is involved in differential phosphoinositide 3-kinase signaling by two splice forms of c-Kit
Publications: Journal of Biological Chemistry 283.41 : 27444-27451.
Link: http://www.jbc.org/content/283/41/27444.full

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFRSF8 igG-His Human/CYT-983

ProSpec-TNFRSF8 igG-His Human,ProSpec CYT-983,肿瘤坏死因子受体超家族成员 8 是 TNFSF8/CD30L 的受体。 TNFRSF8 在调节活化淋巴母细胞的细胞生长和转化中发挥作用。此外，TNFRSF8 蛋白通过激活 NF-kappa-B 来调节基因表达。

TNFRSF8 igG-His Human

CD30 Ligand Receptor, IgG-His Tag Human Recombinant
CYT-983
ProSpec-TNFRSF8 igG-His Human/CYT-983 2µg
ProSpec-TNFRSF8 igG-His Human/CYT-983 10µg
ProSpec-TNFRSF8 igG-His Human/CYT-983 1mg

Catalogue number

CYT-983

Synonyms

Tumor Necrosis Factor Receptor Superfamily Member 8, Tumor Necrosis Factor Receptor Superfamily, Member 8, Lymphocyte Activation Antigen CD30, CD30L Receptor, Ki-1 Antigen, D1S166E, CD30, Cytokine Receptor CD30, CD30 Antigen, Ki-1.

Introduction

Description

TNFRSF8 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 613 amino acids and having a molecular mass of 66.7kDa .

TNFRSF8 is expressed with a 252 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFRSF8 是单条糖基化多肽链，含有 613 个氨基酸，分子量为 66.7kDa。

TNFRSF8 在 C 末端用 252 个氨基酸的 hIgG-His-tag 表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TNFRSF8 protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。

避免多次冻融循环。

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

ADPFPQDRPF EDTCHGNPSH YYDKAVRRCC YRCPMGLFPT QQCPQRPTDC RKQCEPDYYL DEADRCTACV TCSRDDLVEK TPCAWNSSRV CECRPGMFCS TSAVNSCARC FFHSVCPAGM IVKFPGTAQK NTVCEPASPG VSPACASPEN CKEPSSGTIP QAKPTPVSPA TSSASTMPVR GGTRLAQEAA SKLTRAPDSP SSVGRPSSDP GLSPTQPCPE GSGDCRKQCE PDYYLDEAGR CTACVSCSRD DLVEKTPCAW NSSRTCECRP GMICATSATN SCARCVPYPI CAAETVTKPQ DMAEKDTTFE APPLGTQPDC NPTPENGEAP ASTSPTQSLL VDSQASKTLP IPTSAPVALS STGKAAAFES RACSLEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-TNFRSF8 Mouse/CYT-1165

ProSpec-TNFRSF8 Mouse,ProSpec CYT-1165,肿瘤坏死因子受体超家族成员 8 是 TNFSF8/CD30L 的受体。 TNFRSF8 在调节活化淋巴母细胞的细胞生长和转化中发挥作用。此外，TNFRSF8 蛋白通过激活 NF-kappa-B 来调节基因表达。 TNFRSF8 是细胞凋亡的调节剂，根据细胞类型诱导细胞死亡或增殖。

TNFRSF8 Mouse

CD30 Ligand Receptor Mouse Recombinant
CYT-1165
ProSpec-TNFRSF8 Mouse/CYT-1165 5µg
ProSpec-TNFRSF8 Mouse/CYT-1165 20µg
ProSpec-TNFRSF8 Mouse/CYT-1165 1mg

Catalogue number

CYT-1165

Synonyms

Tumor Necrosis Factor Receptor Superfamily, Member 8, Lymphocyte Activation Antigen CD30, CD30L Receptor, Ki-1 Antigen, D1S166E, CD30, Cytokine Receptor CD30, CD30 Antigen, Ki-1

Introduction

Description

TNFRSF8 Mouse Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 479 amino acids and having a molecular mass of 52.2kDa.
TNFRSF8 is fused to a 239 amino acid hIgG-His tag at C-terminus and purified by proprietary chromatographic techniques.

杆状病毒产生的TNFRSF8小鼠重组体是一条糖基化多肽链，含有479个氨基酸，分子量为52.2kDa。
TNFRSF8 在 C 端与 239 个氨基酸的 hIgG-His 标签融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TNFRSF8 protein contains 10% glycerol and Phosphate-Buffered Saline .

Stability

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

FPTDRPLKTT CAGDLSHYPG EAARNCCYQC PSGLSPTQPC PRGPAHCRKQ CAPDYYVNED GKCTACVTCL PGLVEKAPCS GNSPRICECQ PGMHCCTPAV NSCARCKLHC SGEEVVKSPG TAKKDTICEL PSSGSGPNCS NPGDRKTLTS HATPQAMPTL ESPANDSARS LLPMRVTNLV QEDATELVKV PESSSSKARE PSPDPGNAEK NMTLELPSPG TLPDISTSEN SKEPASTAST LEPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGKHHHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-SCF Human, HEK

Description

Catalogue number

CYT-111

Synonyms

Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.

Introduction

Description

SCF Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 35-45kDa due to glycosylation.
The SCF is purified by proprietary chromatographic techniques.

Source

HEK.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

SCF was lyophilized from a 0.2µm filtered solution containing 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized SCF in sterile PBS containing 0.1% endotoxin-free recombinant HSA.

Stability

Purity

Greater than 95% as obsereved by -PAGE.

Biological Activity

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells .
The EC₅₀ is 15.25ng/ml.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFSF12 Human/CYT-699

ProSpec-TNFSF12 Human,ProSpec CYT-699,冻干的 TNFSF12 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。重组后，TWEAK 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

TNFSF12 Human

TNF Ligand Superfamily Member 12 Human Recombinant
CYT-699
ProSpec-TNFSF12 Human/CYT-699 5µg
ProSpec-TNFSF12 Human/CYT-699 25µg
ProSpec-TNFSF12 Human/CYT-699 1mg

Catalogue number

CYT-699

Synonyms

TWEAK, TNF-related weak inducer of apoptosis, TNFSF12, DR3LG, Apo3-Ligand, APO3L, TNFRSF12A, Tumor necrosis factor ligand superfamily member 12, MGC20669, MGC129581.

Introduction

TNFSF12 is a cytokine that is part of the TNF ligand family. TNFSF12 is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TNFSF12 induces apoptosis through multiple pathways of cell death in a cell type-specific manner. TNFSF12 promotes proliferation and migration of endothelial cells, and therefore acts as a regulator of angiogenesis. TNFSF12 is expressed in adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK h induces NFkB and chemokine secretion. TNFSF12 exerts an apoptotic activity in HT-29 human adenocarcinoma cells whilst cultured in the presence of IFN-?. TNFSF12 promotes proliferation and migration of endothelial cells.

TNFSF12 是一种细胞因子，是 TNF 配体家族的一部分。 TNFSF12 是 FN14/TWEAKR 受体的配体。 TNFSF12 具有与 TNF 重叠的信号功能，但显示出更广泛的组织分布。 TNFSF12 通过多种细胞死亡途径以细胞类型特异性方式诱导细胞凋亡。 TNFSF12 促进内皮细胞的增殖和迁移，因此充当血管生成的调节剂。 TNFSF12 在成人心脏、胰腺、骨骼肌、小肠、脾脏和外周血淋巴细胞中表达。 TWEAK h 诱导 NFkB 和趋化因子分泌。在存在 IFN-α 的情况下培养时，TNFSF12 在 HT-29 人腺癌细胞中发挥凋亡活性。 TNFSF12 促进内皮细胞的增殖和迁移。

Description

TNFSF12 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 156 amino acids and having a total molecular mass of 18kDa.

The TNFSF12 is fused with an 8 amino acids his tag at N-terminal and purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFSF12 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 156 个氨基酸，总分子量为 18kDa。

TNFSF12 在 N 端融合了 8 个氨基酸的 his 标签，并通过专有色谱技术进行纯化。

Source

Escherichia coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2μm filtered solution in PBS.

Solubility

It is recommended to reconstitute the lyophilized TNFSF12 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 TNFSF12，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNFSF12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TWEAK should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein .

冻干的 TNFSF12 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。重组后，TWEAK 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

对于长期储存，建议添加载体蛋白。

Please prevent freeze-thaw cycles.

Amino acid sequence

MHHHHHHRSA PKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH.

Purity

Greater than 95% as determined by -PAGE.

Biological Activity

The ED₅₀, as measured in a proliferation assay using HUVECs, is less than 8ng/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-SCF Human, Sf9

Description

Catalogue number

CYT-421

Synonyms

Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.

Introduction

Stem cell factor / KIT ligand is a cytokine which binds CD117. SCF is also known as “steel factor” or “c-kit ligand”. SCF exists in two forms, cell surface bound SCF and soluble SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.
SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E .

Description

Stem Cell Factor Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.
The SCF is fused to a C-terminal His-tag and purified by proprietary chromatographic techniques.

Source

Sf9, Insect Cells.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein is supplied in 1xPBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized Stem Cell Factor in 10mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by analysis by -PAGE.

Biological Activity

The ED₅₀ as determined by the dose-dependent stimulation of Human TF-1 cells is typically 1-5 ng/ml.

Protein content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-SCF Mouse

Description

Catalogue number

CYT-275

Synonyms

Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250, Hematopoietic growth factor KL, Steel factor.

Introduction

Stem cell factor / KIT ligand is a cytokine which binds CD117. SCF is also known as “steel factor” or “c-kit ligand”. SCF exists in two forms, cell surface bound SCF and soluble SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.
SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E .

Description

Stem Cell Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18309 Dalton.
The SCF is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a concentrated solution in water containing 0.02% NaHCO3.

Solubility

It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Purity

Greater than 98.0% as determined by:
Analysis by SEC-HPLC.
Analysis by -PAGE.

Amino acid sequence

MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of Human TF-1 cell line is < 10 ng/ml, corresponding to a Specific Activity of 1×10⁵IU/mg.

Protein content

References

1.Title:enhanced self -renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene sall4.
Publication:
Link:http://www.biomedcentral.com/content/pdf/1756-8722-4-38.pdf

2.Title:Contribution of an Aged Microenvironment to Aging-Associated Myeloproliferative Disease
Publication:Vas V, Wandhoff C, D?rr K, Niebel A, Geiger H Contribution of an Aged Microenvironment to Aging-Associated Myeloproliferative Disease. PLoS ONE 7: e31523. doi:10.1371/journal.pone.0031523
Link:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0031523

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFSF14 Human/CYT-1202

ProSpec-TNFSF14 Human,ProSpec CYT-1202,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,TNFRSF14 是 TNF 受体超家族的成员，是一种 I 型跨膜蛋白。 TNFRSF14 在外周血 T 细胞、B 细胞、单核细胞和富含淋巴细胞的各种组织中表达。

ProSpec-TNFSF14 Human/CYT-1202

Catalogue number

CYT-1202

Synonyms

Tumor necrosis factor ligand superfamily member 14, CD258, Tnfsf14, Light

Introduction

Description

Recombinant Human LIGHT having a Mw of 43kDa was purified from E. coli.
The Recombinant Human LIGHT is fused to GST tag at its N terminal and purified by proprietary chromatographic technique.

从大肠杆菌中纯化具有 43kDa 分子量的重组人 LIGHT。
Recombinant Human LIGHT 在其 N 末端与 GST 标签融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNFSF14 solution contains 25mM Tris-Base/ 25mM K₂CO_3.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Amino acid sequence

DGPAGSWEQL IQERRSHEVN PAAHLTGANS SLTGSGGPLL WETQLGLAFL RGLSYHDGAL VVTKAGYYYI YSKVQLGGVG CPLGLASTIT HGLYKRTPRY PEELELLVSQ QSPCGRATSS SRVWWDSSFL GGVVHLEAGE KVVVRVLDER LVRLRDGTRS YFGAFMV

Purity

Protein is >95% pure as determined by 10% PAGE .

Applications

Immunoassay.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFSF14 Mouse/CYT-826

ProSpec-TNFSF14 Mouse,ProSpec CYT-826,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,TNFRSF14 是 TNF 受体超家族的成员，是一种 I 型跨膜蛋白。 TNFRSF14 在外周血 T 细胞、B 细胞、单核细胞和富含淋巴细胞的各种组织中表达。

TNFSF14 Mouse

LIGHT Mouse Recombinant
CYT-826
ProSpec-TNFSF14 Mouse/CYT-826 5µg
ProSpec-TNFSF14 Mouse/CYT-826 20µg
ProSpec-TNFSF14 Mouse/CYT-826 1mg

Catalogue number

CYT-826

Synonyms

Tumor necrosis factor ligand superfamily member 14, CD258, Tnfsf14, Light.

Introduction

Description

TNFSF14 Mouse Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 168 amino acids and having a molecular mass of 18.4kDa.

在大肠杆菌中产生的 TNFSF14 Mouse Recombinant 是一种单一的、非糖基化的多肽链，含有 168 个氨基酸，分子量为 18.4kDa。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

TNFSF14 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS pH7.4.

Solubility

It is recommended to reconstitute the lyophilized TNFSF14 in sterile 100mM HAc not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的 TNFSF14 溶解在不低于 100µg/ml 的无菌 100mM HAc 中，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNFSF14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFSF14 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFSF14 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。重组后 TNFSF14 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 96.0% as determined by:

Analysis by RP-HPLC.

Analysis by -PAGE.

Amino acid sequence

DGGKGSWEKL IQDQRSHQAN PAAHLTGANA SLIGIGGPLL WETRLGLAFL RGLTYHDGAL VTMEPGYYYV YSKVQLSGVG CPQGLANGLP ITHGLYKRTS RYPKELELLV SRRSPCGRAN SSRVWWDSSF LGGVVHLEAG EEVVVRVPGN RLVRPRDGTR SYFGAFMV.

Biological Activity

The ED₅₀ as determined by a cytotoxicity assay using human HT-29 cells is less than 2µg/ml, corresponding to a specific activity of > 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and rHuIFN-g.

通过使用人 HT-29 细胞的细胞毒性测定法确定的 ED50 小于 2μg/ml，对应于在鼠抗多组氨酸单克隆抗体和 rHuIFN-g 存在下 > 500 IU/mg 的比活性。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-SCF Rat

Description

Catalogue number

CYT-323

Synonyms

Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250, Hematopoietic growth factor KL.

Introduction

Stem cell factor / KIT ligand is a cytokine which binds CD117 . SCF is also known as “steel factor” or “c-kit ligand”. SCF exists in two forms, cell surface bound SCF and soluble SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.
SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E .

Description

Stem cell factor Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 164 amino acids and having a molecular mass of 18.4 kDa.
The Rat SCF is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a concentrated solution in water containing 0.02% NaHCO3.

Solubility

It is recommended to reconstitute the lyophilized SCF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized rat SCF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by:
Analysis by SEC-HPLC.
Analysis by -PAGE.

Amino acid sequence

MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.

Biological Activity

The ED50 is determined by the dose-dependant stimulation of the proliferation of human TF-1 cells which is < 10 ng/ml, corresponding to a specific activity of 100,000units/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TGFA Human

Description

Catalogue number

CYT-871

Synonyms

Transforming Growth Factor Alpha, Protransforming Growth Factor Alpha, TGF-Alpha, TGFA.

Introduction

Transforming Growth Factor-Alpha belongs to the EGF family of cytokines. TGFA soluble form is discharged from the membrane by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and has a role in cell-cell adhesion or in the stimulation of adjacent cells. TGFA expression is common in transformed cells. Additionally, TGFA is expressed in normal tissues during embryogenesis and in adult cells/tissues, including the pituitary, keratinocytes, and macrophages.

Description

TGFA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 5.6kDa. The TGFA is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered solution in 0.1% TFA.

Solubility

It is recommended to reconstitute the lyophilized TGFA in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TGFA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFA should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA.

Biological Activity

The ED₅₀, as measured in a proliferation assay using mouse BALB/c 3T3 cells, is 0.395ng/ml.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFSF7 Human/CYT-880

ProSpec-TNFSF7 Human,ProSpec CYT-880,CD70 也称为 TNFSF7，是一种与 CD27 结合的细胞因子。 TNFSF7 参与 T 细胞活化并诱导共刺激 T 细胞增殖。此外，TNFSF7 增强了溶细胞性 T 细胞的产生。在与 TNFSF7 相关的疾病中：包括急性心肌炎和心肌炎。

TNFSF7 Human

CD70 Human Recombinant
CYT-880
ProSpec-TNFSF7 Human/CYT-880 5µg
ProSpec-TNFSF7 Human/CYT-880 20µg
ProSpec-TNFSF7 Human/CYT-880 1mg

Catalogue number

CYT-880

Synonyms

CD70 Molecule, TNFSF7, CD27L, Tumor Necrosis Factor Superfamily, Member 7, Tumor Necrosis Factor Ligand Superfamily Member 7, CD70 Antigen, CD27 Ligand, CD27LG, CD27-L, Surface Antigen CD70, Ki-24 Antigen, CD70 antigen.

Introduction

CD70 also known as TNFSF7 is a cytokine which binds to CD27. TNFSF7 takes part in T-cell activation as well as induces the proliferation of costimulated T-cells. Moreover, TNFSF7 enhances the generation of cytolytic T-cells. Among the diseases which are associated with TNFSF7: Include acute myocarditis & Myocarditis.

CD70 也称为 TNFSF7，是一种与 CD27 结合的细胞因子。 TNFSF7 参与 T 细胞活化并诱导共刺激 T 细胞增殖。此外，TNFSF7 增强了溶细胞性 T 细胞的产生。在与 TNFSF7 相关的疾病中：包括急性心肌炎和心肌炎。

Description

TNFSF7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 178 amino acids and having a molecular mass of 19.5kDa.

TNFSF7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFSF7 Human Recombinant 是单条非糖基化多肽链，包含 178 个氨基酸，分子量为 19.5kDa。

TNFSF7 在 N 端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

TNFSF7 protein solution containing 20mM Tris-HCl buffer and 10% glycerol.

Stability

Purity

Greater than 80% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSQRFAQAQ QQLPLESLGW DVAELQLNHT GPQQDPRLYW QGGPALGRSF LHGPELDKGQ LRIHRDGIYM VHIQVTLAIC SSTTASRHHP TTLAVGICSP ASRSISLLRL SFHQGCTIAS QRLTPLARGD TLCTNLTGTL LPSRNTDETF FGVQWVRP.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TGFB1 (113 a.a.) Human

Description

Catalogue number

CYT-679

Synonyms

Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1.

Introduction

Description

TGF-b 1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 113 amino acids and having a total molecular mass of 12.9 kDa.
TGF-b 1 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The TGF-b 1 solution contains 10mM Sodium Citrate and 10% glycerol.

Stability

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MALDTNYCFS STEKNCCVRQ LYIDFRKDLG WKWIHEPKGY HANFCLGPCP YIWSLDTQYS KVLALYNQHN PGASAAPCCV PQALEPLPIVYYVGRKPKVE QLSNMIVRSC KCS.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFSF7 Human, sf9/CYT-953

TNFSF7 Human, sf9
CD70 Human Recombinant, sf9
CYT-953
ProSpec-TNFSF7 Human, sf9/CYT-953 2µg
ProSpec-TNFSF7 Human, sf9/CYT-953 10µg
ProSpec-TNFSF7 Human, sf9/CYT-953 1mg

Catalogue number

CYT-953

Synonyms

CD70 Molecule, Tumor Necrosis Factor Superfamily, Member 7, Tumor Necrosis Factor Ligand Superfamily Member 7 , CD27 Ligand, CD27LG, TNFSF7, CD27-L, CD27L, Tumor Necrosis Factor Ligand 8A, Surface Antigen CD70, Ki-24 Antigen, CD70 Antigen, TNLG8A.

Introduction

Description

TNFSF7 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 164 amino acids and having a molecular mass of 18.2kDa.

TNFSF7 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFSF7 是单条糖基化多肽链，包含 164 个氨基酸，分子量为 18.2kDa。
TNFSF7 在 C 末端带有 6 个氨基酸的 His 标签，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TNFSF7 protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。

对于长期储存，建议添加载体蛋白。

避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

ADPQRFAQAQ QQLPLESLGW DVAELQLNHT GPQQDPRLYW QGGPALGRSF LHGPELDKGQ LRIHRDGIYM VHIQVTLAIC SSTTASRHHP TTLAVGICSP ASRSISLLRL SFHQGCTIAS QRLTPLARGD TLCTNLTGTL LPSRNTDETF FGVQWVRPHH HHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFSF8 Human/CYT-824

ProSpec-TNFSF8 Human,ProSpec CYT-824,肿瘤坏死因子配体超家族成员 8、CD30 配体、CD30-L、CD153、TNFSF8、CD30L、CD30LG、CD30 抗原配体、CD153 抗原。

TNFSF8 Human
CD30 Ligand Human Recombinant
CYT-824
ProSpec-TNFSF8 Human/CYT-824 5µg
ProSpec-TNFSF8 Human/CYT-824 25µg
ProSpec-TNFSF8 Human/CYT-824 1mg

Catalogue number

CYT-824

Synonyms

Tumor necrosis factor ligand superfamily member 8, CD30 ligand, CD30-L, CD153, TNFSF8, CD30L, CD30LG, CD30 Antigen Ligand, CD153 Antigen.

肿瘤坏死因子配体超家族成员 8、CD30 配体、CD30-L、CD153、TNFSF8、CD30L、CD30LG、CD30 抗原配体、CD153 抗原。

Introduction

CD30 Ligand is a cytokine which is a member of the tumor necrosis factor ligand family. The TNFSF8 cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The employment of the TNFSF8 cytokine expressed on B cell surface has an inhibitory role in modulating Ig class switch. TNFSF8 enhances cell proliferation of some lymphoma cell lines, while inducing cell death and reducing cell proliferation of other lymphoma cell lines. The pleiotropic biological activities of the TNFSF8 cytokine on different CD30+ lymphoma cell lines has a pathophysiologic role in Hodgkin’s and some non-Hodgkin’s lymphomas.

CD30 配体是一种细胞因子，是肿瘤坏死因子配体家族的成员。 TNFSF8 细胞因子是 TNFRSF8/CD30 的配体，TNFRSF8/CD30 是细胞表面抗原，也是霍奇金淋巴瘤和相关血液恶性肿瘤的标志物。 B 细胞表面表达的 TNFSF8 细胞因子的使用在调节 Ig 类转换中具有抑制作用。 TNFSF8增强一些淋巴瘤细胞系的细胞增殖，同时诱导细胞死亡并减少其他淋巴瘤细胞系的细胞增殖。 TNFSF8 细胞因子对不同 CD30+ 淋巴瘤细胞系的多效生物学活性在霍奇金和一些非霍奇金淋巴瘤中具有病理生理作用。

Description

TNFSF8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 195 amino acids and having a molecular mass of 22kDa.
TNFSF8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFSF8 Human Recombinant 是一种单一的、非糖基化的多肽链，含有 195 个氨基酸，分子量为 22kDa。
TNFSF8 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNFSF8 protein solution contains 20mM Tris-HCl buffer , 10% glycerol and 0.4M Urea.

Stability

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
请避免冻融循环。

Purity

Greater than 80% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSQRTDSIP NSPDNVPLKG GNCSEDLLCI LKRAPFKKSW AYLQVAKHLN KTKLSWNKDG ILHGVRYQDG NLVIQFPGLY FIICQLQFLV QCPNNSVDLK LELLINKHIK KQALVTVCES GMQTKHVYQN LSQFLLDYLQ VNTTISVNVD TFQYIDTSTF PLENVLSIFL YSNSD.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-TGFB1 Human

Description

Catalogue number

CYT-561

Synonyms

Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1.

Introduction

Description

Human Transforming Growth Factor-beta 1 purified from Human Platelets having a molecular mass of 25kDa.
The TGF-b 1 is purified by proprietary chromatographic techniques.

Source

Human Platelets.

Physical Appearance

Sterile Filtered lyophilized powder.

Formulation

TGF-Beta1 protein was lyophilized from a solution containing 30% acetonitrile and 0.1% trifluoroacetic acid.

Solubility

It is recommended to reconstitute lyophilized TGF-beta 1 in 0.5% BSA in 0.1N acetic acid, which can then be further diluted to the desired aliquot with 30% acetonitrile and 0.1% trifluoroacetic acid.

Stability

Lyophilized TGF-beta 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGF-beta 1 should be stored at 4°C between 2-7 days and for future use below -18°C.
DO NOT RECONSTITE WITH NEUTRAL BUFFERS.
DO NOT USE GLASS IMPLEMENTS OR EXTENSIVE MANIPULATIONS.
PREVENT FREEZE THAW CYCLES.

Purity

Greater than 98.0% as determined by -PAGE.

Biological Activity

Stimulates the growth of NRK-1 cells in soft agar at concentrations ranging from 0.1 to 5ng/ml corresponding to a specific activity of 200,000-10,000,000IU/mg. Effective concentration ranges must be experimentally determined. Purified EGF and/or TGF- must be present for observation of the biological activity.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TGFB1 Human Recombinant

Description

Catalogue number

CYT-716

Synonyms

Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.

Introduction

Description

TGFB1 Human Recombinant produced in CHO cells is a glycosylated homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25.6kDa. The TGFB1 is purified by proprietary chromatographic techniques.

Source

CHO cells.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a sterile filtered solution containing 0.1 % trifluoroacetic acid And trehalose .

Solubility

It is recommended to reconstitute the lyophilized TGFB1 in sterile 10mM HCl at a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB1 Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein . Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.

Biological Activity

The ED₅₀ as determined by the dose-dependent inhibition of IL-4-induced proliferation of HT-2 cells is 0.0149ng/ml, corresponding to a specific activity of 6.7×10⁷units/mg.

References

1.Title: attenuates skeletal muscle dystrophy in mdx mice .
Publication:Published online before print March 16, 2009, doi: 10.1096/fj.09-129833 August 2009 The FASEB Journal vol. 23 no. 8 2539-2548 .
Link:http://www.fasebj.org/content/23/8/2539.full

2.Title:Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins.
Publication:Kallio H, Tolvanen M, J?nis J, Pan P-w, Laurila E, et al. Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins. PLoS ONE 6: e27152. doi:10.1371/journal.pone.0027152
Link:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027152

3.Title: Cancer-Associated Carbonic Anhydrases IX and XII: Effect of Growth Factors on Gene Expression in Human Cancer Cell Lines.

Publication: Journal of Cancer Molecules 5: 73-78, 2010.

Link: http://mupnet.com/JOCM%205%2073-78.pdf

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFSF8 Human, Sf9/CYT-954

ProSpec-TNFSF8 Human Sf9,ProSpec CYT-954,CD30 配体是一种细胞因子，是肿瘤坏死因子配体家族的成员。 TNFSF8 细胞因子是 TNFRSF8/CD30 的配体，TNFRSF8/CD30 是细胞表面抗原，也是霍奇金淋巴瘤和相关血液恶性肿瘤的标志物。

TNFSF8 Human, Sf9
CD30 Ligand Human Recombinant, Sf9
CYT-954
ProSpec-TNFSF8 Human, Sf9/CYT-954 1µg
ProSpec-TNFSF8 Human, Sf9/CYT-954 5µg
ProSpec-TNFSF8 Human, Sf9/CYT-954 50µg

Catalogue number

CYT-954

Synonyms

Tumor Necrosis Factor Superfamily Member 8, Tumor Necrosis Factor Superfamily, Member 8, CD153 Antigen, CD30 Ligand, CD30LG, CD30-L, CD30L, Tumor Necrosis Factor Superfamily Member 8, Tumor Necrosis Factor Ligand 3A, CD30 Antigen Ligand, TNLG3A, CD153, Tumor necrosis factor ligand superfamily member 8, TNFSF8, CD30 ligand.

Introduction

Description

TNFSF8 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 181 amino acids and having a molecular mass of 20.7kDa .
TNFSF8 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFSF8 是单条糖基化多肽链，含有 181 个氨基酸，分子量为 20.7kDa。
TNFSF8 在 C 末端用 6 个氨基酸 His 标签表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

TNFSF8 protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Purity

Greater than 85% as determined by -PAGE.

Amino acid sequence

ADPQRTDSIP NSPDNVPLKG GNCSEDLLCI LKRAPFKKSW AYLQVAKHLN KTKLSWNKDG ILHGVRYQDG NLVIQFPGLY FIICQLQFLV QCPNNSVDLK LELLINKHIK KQALVTVCES GMQTKHVYQN LSQFLLDYLQ VNTTISVNVD TFQYIDTSTF PLENVLSIFL YSNSDHHHHH H.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TGFB1 Human, His

Description

Catalogue number

CYT-672

Synonyms

Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, TGFB1.

Introduction

Transforming growth factor betas mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
TGF-b 1 regulates the actions of numerous other growth factors involved in a variety of human diseases including renal disease, hepatic disease, heart failure and cardiomyopathies.

Description

TGF-b 1 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 112 amino acids fragment having a molecular weight of 17.3kDa and fused with a 4.5kDa amino-terminal hexahistidine tag.
The TGF-b 1 His is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TGF-b 1 His-Tag protein is supplied in 25mM NaAcetate pH 4.8 and 50% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TRAIL Human/CYT-443

ProSpec-TRAIL Human,ProSpec CYT-443,冻干的 APO 2 配体虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。重组后，TRAIL 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

TRAIL Human
TRAIL / APO2 Ligand Human Recombinant
CYT-443
ProSpec-TRAIL Human/CYT-443 10µg
ProSpec-TRAIL Human/CYT-443 50µg
ProSpec-TRAIL Human/CYT-443 1mg

Catalogue number

CYT-443

Synonyms

Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, Apo-2 ligand, Apo-2L, CD253 antigen, TL2, APO2L, TNFSF10.

Introduction

TNF-related apoptosis-inducing ligand is a ligand molecule which induces apoptosis. It is a type II transmembrane protein with homology to other members of the tumor necrosis factor family.
In humans, the gene that encodes for TRAIL is located at chromosome 3q26. TRAIL binds to the death receptors, DR4 and DR5. The process of apoptosis is caspase-8-dependent. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues.

TNF相关的凋亡诱导配体是诱导细胞凋亡的配体分子。它是一种 II 型跨膜蛋白，与肿瘤坏死因子家族的其他成员具有同源性。
在人类中，编码 TRAIL 的基因位于染色体 3q26。 TRAIL 与死亡受体 DR4 和 DR5 结合。细胞凋亡过程是 caspase-8 依赖性的。这种蛋白质优先诱导转化细胞和肿瘤细胞凋亡，但似乎不会杀死正常细胞，尽管它在大多数正常组织中以显着水平表达。

Description

TRAIL/APO 2 Ligand Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 168 amino acids and having a molecular mass of ~21kDa. The sTRAIL is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TRAIL/APO 2 Ligand Human Recombinant 是一种单一的、非糖基化的多肽链，含有 168 个氨基酸，分子量约为 21kDa。 sTRAIL 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a filtered solution containing 20mM Tris-HCl pH 8.0 and 150mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized TRAIL in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中重新配制冻干的 TRAIL，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized APO 2 Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TRAIL should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 APO 2 配体虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。重组后，TRAIL 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG.

Biological Activity

The activity is determined by the cytolysis of Murine L929 cells in the presence of Actinomycin D, ED₅₀ for this effect is less than 2ng/ml, corresponding to a specific activity of 5,000,000IU/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TGFB1 Mouse

Description

Catalogue number

CYT-858

Synonyms

Transforming growth factor beta-1, TGF-beta-1, Tgfb, Tgfb-1, TGFbeta1.

Introduction

Transforming growth factor betas mediate many cell-cell interactions which occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins which are very similar in that each is cleaved to yield a 112 amino acid polypeptide which remains associated with the latent portion of the molecule.

Description

TGFB1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15.2kDa.

TGFB1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TGFB1 protein solution containing 20mM Tris-HCl and 10% glycerol.

Stability

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSALDTNYC FSSTEKNCCV RQLYIDFRKD LGWKWIHEPK GYHANFCLGP CPYIWSLDTQ YSKVLALYNQ HNPGASASPC CVPQALEPLP IVYYVGRKPK VEQLSNMIVR SCKCS.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TRAIL Human (114-281 a.a.)/CYT-546

ProSpec-TRAIL Human (114-281 a.a.),ProSpec CYT-546,Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, Apo-2 ligand, Apo-2L, CD253 antigen, TL2, APO2L, TNFSF10.

TRAIL Human

TRAIL/APO 2 Ligand Human Recombinant
CYT-546
ProSpec-TRAIL Human (114-281 a.a.)/CYT-546 10µg
ProSpec-TRAIL Human (114-281 a.a.)/CYT-546 50µg
ProSpec-TRAIL Human (114-281 a.a.)/CYT-546 1mg

Catalogue number

CYT-546

Synonyms

Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, Apo-2 ligand, Apo-2L, CD253 antigen, TL2, APO2L, TNFSF10.

Introduction

TNF-related apoptosis-inducing ligand is a ligand molecule which induces apoptosis. It is a type II transmembrane protein with homology to other members of the tumor necrosis factor family.
In humans, the gene that encodes for TRAIL is located at chromosome 3q26.
TRAIL binds to the death receptors, DR4 and DR5. The process of apoptosis is caspase-8-dependent. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues.

TNF相关的凋亡诱导配体是诱导细胞凋亡的配体分子。它是一种 II 型跨膜蛋白，与肿瘤坏死因子家族的其他成员具有同源性。
在人类中，编码 TRAIL 的基因位于染色体 3q26。
TRAIL 与死亡受体 DR4 和 DR5 结合。细胞凋亡过程是 caspase-8 依赖性的。这种蛋白质优先诱导转化细胞和肿瘤细胞凋亡，但似乎不会杀死正常细胞，尽管它在大多数正常组织中以显着水平表达。

Description

Soluble TNF-related apoptosis-inducing ligand Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 169 amino acids and having a molecular mass of 19.6 kDa. The sTRAIL is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的可溶性 TNF 相关凋亡诱导配体 Human Recombinant 是一种单一的、非糖基化的多肽链，含有 169 个氨基酸，分子量为 19.6 kDa。 sTRAIL 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless liquid.

Formulation

1mg/ml in 20mM Tris-HCl pH-7.5, 300mM NaCl, 0.1mM DTT & 10% glycerol.

Stability

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MVRERGPQRV AAHITGTRGR SNTLSSPNSK NEKALGRKIN SWESSRSGHS FLSNLHLRNGELVIHEKGFY YIYSQTYFRF QEEIKENTKN DKQMVQYIYK YTSYPDPILL MKSARNSCWSKDAEYGLYSI YQGGIFELKE NDRIFVSVTN EHLIDMDHEA SFFGAFLVG.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TGFB2 Human

Description

Catalogue number

CYT-441

Synonyms

Transforming growth factor, beta 2, cetermin, Glioblastoma-derived T-cell suppressor factor, polyergin, G-TSF, TGF-beta2, TGF-beta-2, transforming growth factor beta-2, BSC-1 cell growth inhibitor, TGFB-2.

Introduction

TGFB2 is a 27.08 kDa protein having two identical 118 amino acid peptide chains linked by a single disulfide bond. TGFB2 is part of a family of five related cytokines that have an extensive variation of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-β signal through the same receptor and stimulate similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing.

Description

TGFB2 Human Recombinant produced in plants is a homodimeric polypeptide chain containing 2 x 118 amino acids and having a total molecular mass of 27.08kDa. The TGFB2 is fused to 6xHis Tag at N-terminus and purified by proprietary chromatographic techniques.

Source

Nicotiana benthamiana.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a concentrated solution containing 50mM Tris-HCl pH-7.4.

Solubility

It is recommended to reconstitute the lyophilized TGFB2 in sterile 18M-cm H₂O not less than 1µg/40µl, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TGFB2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB2 Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein . Please prevent freeze-thaw cycles.

Purity

Greater than 97.0% as determined by -PAGE.

Amino acid sequence

HHHHHHALDAAYCFRNVQDNCCLRPLYIDFKRDLGWKWIH
EPKGYNANFCAGACPYLWSSDTQHSRVLSLYNTINPEASAS
PCCVSQDLEPLTI LYYIGKTPKIEQLSNMIVKSCKCS.

Biological Activity

The biological activity of TGFB2 is measured in culture by its ability to inhibit the mink lung epithelial cells proliferation. ED50 < 40ng/ml, corresponding to a specific activity of 25,000 units/mg.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TRAIL Human (114-281 a.a.), Active/CYT-1014

ProSpec-TRAIL Human (114-281 a.a.) Active,ProSpec CYT-1014,TNF相关的凋亡诱导配体是诱导细胞凋亡的配体分子。它是一种 II 型跨膜蛋白，与肿瘤坏死因子家族的其他成员具有同源性。在人类中，编码 TRAIL 的基因位于染色体 3q26。

TRAIL Human , Active

TRAIL/APO 2 Ligand Human Recombinant, Active
CYT-1014
ProSpec-TRAIL Human (114-281 a.a.), Active/CYT-1014 2µg
ProSpec-TRAIL Human (114-281 a.a.), Active/CYT-1014 10µg
ProSpec-TRAIL Human (114-281 a.a.), Active/CYT-1014 100µg

Catalogue number

CYT-1014

Synonyms

Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, Apo-2 ligand, Apo-2L, CD253 antigen, TL2, APO2L, TNFSF10.

Introduction

TNF-related apoptosis-inducing ligand is a ligand molecule which induces apoptosis. It is a type II transmembrane protein with homology to other members of the tumor necrosis factor family.
In humans, the gene that encodes for TRAIL is located at chromosome 3q26.
TRAIL binds to the death receptors, DR4 and DR5. The process of apoptosis is caspase-8-dependent. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues.

TNF相关的凋亡诱导配体是诱导细胞凋亡的配体分子。它是一种 II 型跨膜蛋白，与肿瘤坏死因子家族的其他成员具有同源性。
在人类中，编码 TRAIL 的基因位于染色体 3q26。
TRAIL 与死亡受体 DR4 和 DR5 结合。细胞凋亡过程是 caspase-8 依赖性的。这种蛋白质优先诱导转化细胞和肿瘤细胞凋亡，但似乎不会杀死正常细胞，尽管它在大多数正常组织中以显着水平表达。

Description

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless liquid.

Formulation

TRAIL protein solution containing 20mM Tris-HCl pH-7.5, 300mM NaCl, 0.1mM DTT & 10% glycerol.

Stability

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Biological Activity

Measured in a cell cytotoxicity assay using Jurkat human T lymphocyte. The ED50 for this effect is less or equal to 1 ng/ml.

使用 Jurkat 人 T 淋巴细胞在细胞毒性试验中测量。这种效应的 ED50 小于或等于 1 ng/ml。

Amino acid sequence

MVRERGPQRV AAHITGTRGR SNTLSSPNSK NEKALGRKIN SWESSRSGHS FLSNLHLRNGELVIHEKGFY YIYSQTYFRF QEEIKENTKN DKQMVQYIYK YTSYPDPILL MKSARNSCWSKDAEYGLYSI YQGGIFELKE NDRIFVSVTN EHLIDMDHEA SFFGAFLVG.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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