分类： ProSpec

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, LT, TNFB, TNFSF1.

Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.

Lymphotoxin alpha 是肿瘤坏死因子家族的成员，是一种由淋巴细胞产生的细胞因子。 LTA 具有高度诱导性、分泌性，并以同源三聚体分子的形式存在。 LTA 与淋巴毒素-β 形成异源三聚体，将淋巴毒素-α 锚定到细胞表面。 LTA 介导多种炎症、免疫刺激和抗病毒反应。 LTA 还参与发育过程中次级淋巴器官的形成，并在细胞凋亡中发挥作用。

Tumor Necrosis Factor-b Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 18645 Dalton.
The TNF-b is purified by standard chromatographic techniques.

在大肠杆菌中产生的肿瘤坏死因子-b 人重组体是一种单一的、非糖基化的多肽链，含有 172 个氨基酸，分子量为 18645 道尔顿。
TNF-b通过标准色谱技术纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized protein with no additives.

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-beta in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized Tumor Necrosis Factor-b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的肿瘤坏死因子-b 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后 TNF-b 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAG.

MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05ng/ml, corresponding to a Specific Activity of 20,000,000IU/mg.

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.082 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a calibrated solution of TNF-b as a Reference Standard.

蛋白质定量通过两种独立的方法进行：
1. 280 nm 的紫外光谱，使用 1.082 的吸光度值作为 0.1% 溶液的消光系数。 该值是由 PC GENE 计算机蛋白质序列分析程序计算得出的。
2. 通过 RP-HPLC 分析，使用 TNF-b 的校准溶液作为参考标准。

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, LT, TNFB, TNFSF1.

TNF-b is a potent multifunctional cytokine produced mainly by activated T and B lymphocytes. The protein has been shown to have a specific potent cytotoxic activity on tumor cells and a variety of other target cells as well as being a mediator of inflammation and immune function.

TNF-b 是一种有效的多功能细胞因子，主要由活化的 T 和 B 淋巴细胞产生。 该蛋白质已被证明对肿瘤细胞和多种其他靶细胞具有特定的有效细胞毒活性，并且是炎症和免疫功能的介质。

TNF-b Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 192 amino acids and having a molecular mass of 20.9kDa.
TNF-b is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNF-b Human Recombinant 是一种单一的、非糖基化的多肽链，含有 192 个氨基酸，分子量为 20.9kDa。
TNF-b 在 N 端与 21 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered clear solution.

TNF-b protein solution contains 20mM Tris-HCl buffer , 30% glycerol, 0.1M NaCl and 1mM DTT.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Greater than 90% as determined by -PAGE.

MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is <0.3 ng/ml.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, LT, TNFB, TNFSF1.

Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.

Lymphotoxin alpha 是肿瘤坏死因子家族的成员，是一种由淋巴细胞产生的细胞因子。 LTA 具有高度诱导性、分泌性，并以同源三聚体分子的形式存在。 LTA 与淋巴毒素-β 形成异源三聚体，将淋巴毒素-α 锚定到细胞表面。 LTA 介导多种炎症、免疫刺激和抗病毒反应。 LTA 还参与发育过程中次级淋巴器官的形成，并在细胞凋亡中发挥作用。

Tumor Necrosis Factor-beta Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 180 amino acids and having a molecular mass of 19.7kDa .
TNFB is fused with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.

Sf9杆状病毒细胞产生的Tumor Necrosis Factor-beta Human Recombinant是一条单一的糖基化多肽链，包含180个氨基酸，分子量为19.7kDa。
TNFB 在 C 末端与 6 个氨基酸 His 标签融合，并通过专有色谱技术进行纯化。

Sf9, Baculovirus cells.

Sterile filtered colorless solution.

TNFB protein solution contains Phosphate Buffered Saline and 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Greater than 90.0% as determined by -PAGE.

Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is ≤ 1ng/ml.

ADPLPGVGLT PSAAQTARQH PKMHLAHSTL KPAAHLIGDP SKQNSLLWRA NTDRAFLQDG FSLSNNSLLV PTSGIYFVYS QVVFSGKAYS PKATSSPLYL AHEVQLFSSQ YPFHVPLLSS QKMVYPGLQE PWLHSMYHGA AFQLTQGDQL STHTDGIPHL VLSPSTVFFG AFALHHHHHH.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Prospec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Tumor necrosis factor is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is involved in systemic inflammationand secreted mainly by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

肿瘤坏死因子是所有刺激急性期反应的一组细胞因子中的一员。 TNF参与全身炎症反应，主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

TNFA Bovine produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 17.5kDa.

TNFA is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFA 牛是一条单一的、非糖基化的多肽链，包含 158 个氨基酸，分子量为 17.5kDa。

TNFA 是通过专有的色谱技术纯化的。

Escherichia Coli.

Sterile filtered colorless solution.

The TNFA contains Phosphate buffer saline and 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Greater than 90.0% as determined by -PAGE.

The ED50 Is < 15 ng/ml and is measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

MLRSSSQASS NKPVAHVVAD INSPGQLRWW DSYANALMAN GVKLEDNQLV VPADGLYLIY SQVLFRGQGC PSTPLFLTHT ISRIAVSYQT KVNILSAIKS PCHRETPEWA EAKPWYEPIY QGGVFQLEKG DRLSAEINLP DYLDYAESGQ VYFGIIAL. 

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Tnfa, Tnfsf2, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2.

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Sf9, Baculovirus cells.

Sterile filtered colorless solution.

TNFA protein solution contains Phosphate buffered saline .

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Greater than 90.0% as determined by -PAGE.

LRSSSQNSSD KPVAHVVANH QVEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLVYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV NLLSAVKSPC PKDTPEGAEL KPWYEPIYLG GVFQLEKGDQ LSAEVNLPKY LDFAESGQVY FGVIALHHHH HH

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Tumor Necrosis Factor-alpha, TNF a His,  Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, N-terminal fragment, NTF, Intracellular domain 1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnfa, Tnfsf2, RATTNF, Tnfa.

肿瘤坏死因子-α、TNF a His、Cachectin、TNF-α、肿瘤坏死因子配体超家族成员 2、TNF-a、N 端片段、NTF、细胞内结构域 1、细胞内结构域 2、ICD2、C 结构域 1、 C 域 2，肿瘤坏死因子，可溶性形式，Tnfa，Tnfsf2，RATTNF，Tnfa。

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

TNFA Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 181 amino acids and having a molecular mass of 19.9kDa.
TNFA Rat is expressed with an 25 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFA Rat Recombinant 是一条单一的、非糖基化的多肽链，含有 181 个氨基酸，分子量为 19.9kDa。
TNFA Rat 在 C 末端用 25 个氨基酸的 His 标签表达，并通过专有色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered colorless liquid.

TNFA Rat protein solution contains Phosphate Buffered Saline , 10% glycerol and 1mM DTT.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Greater than 90.0% as determined by -PAGE.

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is ≤ to 0.2 ng/ml.

MGSSHHHHHH SSGLVPRGSH MGSHMLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

GG2-1; MDC-3.13, SCC-S2, SCCS2, Tumor necrosis factor alpha-induced protein 8, TNF alpha-induced protein 8, Head and neck tumor and metastasis-related protein, NF-kappa-B-inducible DED-containing protein, NDED, TNF-induced protein GG2-1, TNFAIP8.

TNFAIP8 which is a part of the TNFAIP8 family acts as a negative mediator of apoptosis and takes part in tumor progression. TNFAIP8 suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, resulting in inhibition of BID cleavage and activation of caspase-3.

TNFAIP8 是 TNFAIP8 家族的一部分，作为细胞凋亡的负介质并参与肿瘤进展。 TNFAIP8 通过抑制 caspase-8 活性而不是 procaspase-8 的加工来抑制 TNF 介导的细胞凋亡，从而抑制 BID 切割和 caspase-3 的活化。

TNFAIP8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 221 amino acids and having a molecular mass of 25kDa. TNFAIP8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFAIP8 Human Recombinant 是一个单一的、非糖基化的多肽链，含有 221 个氨基酸，分子量为 25kDa。 TNFAIP8 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Escherichia Coli.

Sterile filtered colorless solution.

TNFAIP8 protein solution contains 20mM Tris-HCl buffer , 0.15M NaCl, 10% glycerol and 1mM DTT.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Greater than 90% as determined by -PAGE.

MGSSHHHHHH SSGLVPRGSH MGSMHSEAEE SKEVATDVFN SKNLAVQAQK KILGKMVSKS IATTLIDDTS SEVLDELYRV TREYTQNKKE AEKIIKNLIK TVIKLAILYR NNQFNQDELA LMEKFKKKVH QLAMTVVSFH QVDYTFDRNV LSRLLNECRE MLHQIIQRHL TAKSHGRVNN VFDHFSDCEF LAALYNPFGN FKPHLQKLCD GINKMLDEEN I.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

CD120a, FPF, MS5, p55, p55-R, p60, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR1-d2,TNFR55, TNFR60, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor superfamily member 1A.

TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Human TNFR1 has a major region which controls cell surface expression. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women.
Germline mutations of the extracellular domains of TNFR1 are linked to the autosomal dominant periodic fever syndrome. The impaired receptor clearance is believed to be a mechanism of the disease. Familial hibernian fever is caused by defects in TNFRSF1A gene.

TNFR1 属于 TNF 受体超家族。 TNFR1 是 TNFSF2/TNF-α 和同源三聚体 TNFSF1/淋巴毒素-α 的受体。
有 2 种类型的可溶性 TNF 受体：sTNFR-I 和 sTNFR-II，它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。
TNFR1 能够发出细胞存活和细胞凋亡的信号。 TNFR1 诱导的细胞凋亡需要 2 个连续的信号复合物。 TNFR1 能够激活 NF-kappaB，介导细胞凋亡，并起到炎症调节剂的作用。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNFR1 有助于诱导非杀细胞性 TNF 效应，包括抗病毒状态和酸性鞘磷脂酶的激活。人 TNFR1 具有控制细胞表面表达的主要区域。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。
TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

TNFR Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids and having a molecular mass of 23.6kDa.
TNFR is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFR Human Recombinant 是一个单一的、非糖基化的多肽链，包含 213 个氨基酸，分子量为 23.6kDa。
TNFR 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Escherichia Coli.

Sterile filtered colorless solution.

TNFR protein solution containing 20mM Tirs-HCl and 10% glycerol.

Greater than 85% as determined by -PAGE.

MGSSHHHHHH SSGLVPRGSH MGSIYPSGVI GLVPHLGDRE KRDSVCPQGK YIHPQNNSIC CTKCHKGTYL YNDCPGPGQD TDCRECESGS FTASENHLRH CLSCSKCRKE MGQVEISSCT VDRDTVCGCR KNQYRHYWSE NLFQCFNCSL CLNGTVHLSC QEKQNTVCTC HAGFFLRENE CVSCSNCKKS LECTKLCLPQ IENVKGTEDS GTT.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, TNF-R1, TNF-RI, TNFR-I, p60, p55, CD120a, TNFRSF1A, TNFAR, TNFR1, FPF, TBP1, TNF-R, p55-R, TNFR55, TNFR60, TNF-R-I, TNF-R55, MGC19588.

TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Human TNFR1 has a major region which controls cell surface expression. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women.
Germline mutations of the extracellular domains of TNFR1 are linked to the autosomal dominant periodic fever syndrome. The impaired receptor clearance is believed to be a mechanism of the disease. Familial hibernian fever is caused by defects in TNFRSF1A gene.

TNFR1 属于 TNF 受体超家族。 TNFR1 是 TNFSF2/TNF-α 和同源三聚体 TNFSF1/淋巴毒素-α 的受体。
有 2 种类型的可溶性 TNF 受体：sTNFR-I 和 sTNFR-II，它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。
TNFR1 能够发出细胞存活和细胞凋亡的信号。 TNFR1 诱导的细胞凋亡需要 2 个连续的信号复合物。 TNFR1 能够激活 NF-kappaB，介导细胞凋亡，并起到炎症调节剂的作用。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNFR1 有助于诱导非杀细胞性 TNF 效应，包括抗病毒状态和酸性鞘磷脂酶的激活。人 TNFR1 具有控制细胞表面表达的主要区域。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。
TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

TNFR Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 162 amino acids and having a total molecular mass of 18.2 kDa. TNFR Human Recombinant is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFR Human Recombinant 是单一的、非糖基化的多肽链，包含 162 个氨基酸，总分子量为 18.2 kDa。 TNFR Human Recombinant 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The TNFR protein was lyophilized from 10mM sodium phosphate buffer pH-7.5.

It is recommended to reconstitute the lyophilized TNFR in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TNFR，然后可以进一步稀释成其他水溶液。

Lyophilized TNFR although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFR 虽然在室温下可稳定保存 3 周，但应在 -18C 以下干燥储存。 重组后，TNFR 应在 4C 下储存 2-7 天，以备将来在 -18C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESSGSF TASENHLRHC LSCSKCRKEM GQVEKSSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, TNF-R1, TNF-RI, TNFR-I, p60, p55, CD120a, TNFRSF1A, TNFAR, TNFR1, FPF, TBP1, TNF-R, p55-R, TNFR55, TNFR60, TNF-R-I, TNF-R55, MGC19588.

TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Human TNFR1 has a major region which controls cell surface expression. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women.
Germline mutations of the extracellular domains of TNFR1 are linked to the autosomal dominant periodic fever syndrome. The impaired receptor clearance is believed to be a mechanism of the disease. Familial hibernian fever is caused by defects in TNFRSF1A gene.

TNFR1 属于 TNF 受体超家族。 TNFR1 是 TNFSF2/TNF-α 和同源三聚体 TNFSF1/淋巴毒素-α 的受体。
有 2 种类型的可溶性 TNF 受体：sTNFR-I 和 sTNFR-II，它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。
TNFR1 能够发出细胞存活和细胞凋亡的信号。 TNFR1 诱导的细胞凋亡需要 2 个连续的信号复合物。 TNFR1 能够激活 NF-kappaB，介导细胞凋亡，并起到炎症调节剂的作用。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNFR1 有助于诱导非杀细胞性 TNF 效应，包括抗病毒状态和酸性鞘磷脂酶的激活。人 TNFR1 具有控制细胞表面表达的主要区域。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。
TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

TNFR Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 161 amino acids fragment having a molecular weight of 22.68kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. The TNFR His Tag is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFR Human Recombinant 是一个单一的、非糖基化的多肽链，包含 161 个氨基酸片段，分子量为 22.68kDa，并与 4.5kDa 氨基末端的六组氨酸标签融合。 TNFR His Tag 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered clear solution.

TNFR His Tag protein is supplied in 1xPBS, 50% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.

Greater than 95.0% as determined by -PAGE.

DSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQ
DTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVE
ISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCL
NGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKK
SLECTKLCLPQIEN.

Title: β-Catenin Signaling Pathway Is Crucial for Bone Morphogenetic Protein 2 to Induce New Bone Formation^*

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Tumor Necrosis Factor Receptor Superfamily Member 9, Tumor Necrosis Factor Receptor Superfamily, Member 9, T-Cell Antigen 4-1BB Homolog, 4-1BB Ligand Receptor, T-Cell Antigen ILA, CD137 Antigen, CDw137, CD137, ILA, Interleukin-Activated Receptor, Homolog Of Mouse Ly63,Induced By Lymphocyte Activation , Homolog Of Mouse 4-1BB, Receptor Protein 4-1BB, T Cell Antigen ILA, 4-1BB.

TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Human TNFR1 has a major region which controls cell surface expression. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women.
Germline mutations of the extracellular domains of TNFR1 are linked to the autosomal dominant periodic fever syndrome. The impaired receptor clearance is believed to be a mechanism of the disease. Familial hibernian fever is caused by defects in TNFRSF1A gene.

TNFR1 属于 TNF 受体超家族。 TNFR1 是 TNFSF2/TNF-α 和同源三聚体 TNFSF1/淋巴毒素-α 的受体。
有 2 种类型的可溶性 TNF 受体：sTNFR-I 和 sTNFR-II，它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。
TNFR1 能够发出细胞存活和细胞凋亡的信号。 TNFR1 诱导的细胞凋亡需要 2 个连续的信号复合物。 TNFR1 能够激活 NF-kappaB，介导细胞凋亡，并起到炎症调节剂的作用。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNFR1 有助于诱导非杀细胞性 TNF 效应，包括抗病毒状态和酸性鞘磷脂酶的激活。人 TNFR1 具有控制细胞表面表达的主要区域。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。
TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

Sf9, Baculovirus cells.

Sterile Filtered colorless solution.

TNFR protein solution contains Phosphate Buffered Saline and 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.

Greater than 90.0% as determined by -PAGE.

ADLFERTRSL QDPCSNCPAG TFCDNNRNQI CSPCPPNSFS SAGGQRTCDI CRQCKGVFRT RKECSSTSNA ECDCTPGFHC LGAGCSMCEQ DCKQGQELTK KGCKDCCFGT FNDQKRGICR PWTNCSLDGK SVLVNGTKER DVVCGPSPAD LSPGASSVTP PAPAREPGHS PQLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, TNF-R1, Tumor necrosis factor receptor type I, TNF-RI, TNFR-I, p55, p60, CD120a, Tnfrsf1a, Tnfr-1, Tnfr1, FPF, TNF-R, TNFAR, TNFRI, p55-R, TNFR60, Tnfr-2, TNF-R-I, TNF-R55, TNFRp55, TNF-alphaR1, TNFalpha-R1.

TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Human TNFR1 has a major region which controls cell surface expression. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women.
Germline mutations of the extracellular domains of TNFR1 are linked to the autosomal dominant periodic fever syndrome. The impaired receptor clearance is believed to be a mechanism of the disease. Familial hibernian fever is caused by defects in TNFRSF1A gene.

TNFR1 属于 TNF 受体超家族。 TNFR1 是 TNFSF2/TNF-α 和同源三聚体 TNFSF1/淋巴毒素-α 的受体。
有 2 种类型的可溶性 TNF 受体：sTNFR-I 和 sTNFR-II，它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。
TNFR1 能够发出细胞存活和细胞凋亡的信号。 TNFR1 诱导的细胞凋亡需要 2 个连续的信号复合物。 TNFR1 能够激活 NF-kappaB，介导细胞凋亡，并起到炎症调节剂的作用。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNFR1 有助于诱导非杀细胞性 TNF 效应，包括抗病毒状态和酸性鞘磷脂酶的激活。人 TNFR1 具有控制细胞表面表达的主要区域。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。
TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

TNFR Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 21.1kDa.
The TNFR is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFR Mouse Recombinant 是一条单一的、非糖基化的多肽链，含有 191 个氨基酸，分子量为 21.1kDa。
TNFR 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

TNFR protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

It is recommended to reconstitute the lyophilized TNFR in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TNFR，然后可以进一步稀释成其他水溶液。

Lyophilized TNFR although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFR 虽然在室温下可稳定保存 3 周，但应在 -18C 以下干燥储存。 重组后，TNFR 应在 4C 下储存 2-7 天，以备将来在 -18C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by -PAGE.

IHPSGVTGLV PSLGDREKRD SLCPQGKYVH SKNNSICCTK CHKGTYLVSD CPSPGRDTVC RECEKGTFTA SQNYLRQCLS CKTCRKEMSQ VEISPCQADK DTVCGCKENQ FQRYLSETHF QCVDCSPCFN GTVTIPCKET QNTVCNCHAG FFLRESECVP CSHCKKNEEC MKLCLPPPLA NVTNPQDSGT A.

The ED₅₀ as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 1.0 µg/ml, corresponding to a specific activity of > 1000 IU/mg in the presence of 0.1 ng/mL of rMuTNF-a.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, TNFRSF1B, TNFBR, TNFR2.

TNFR2 belongs to the TNF-receptor superfamily. TNFR2 is receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. TNFR2 mediates the majority of the metabolic effects of TNF-alpha. In addition, knockout studies in mice propose a role for TNFR2 in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2 expression might have a significant role in the angiogenesis, tumor cell proliferation and metastasis of Invasive micropapillary carcinoma of the breast.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women. TNFR2 and TNFR1 form a heterocomplex which mediates the recruitment of 2 anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. IAPs’ function in TNF-receptor signaling is unknown; nevertheless, c-IAP1 is believed to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.

TNFR2 属于 TNF 受体超家族。 TNFR2 是对 TNFSF2/TNF-α 具有高亲和力的受体，对同源三聚体 TNFSF1/淋巴毒素-α 的亲和力低约 5 倍。 TNFR2 介导 TNF-α 的大部分代谢作用。此外，在小鼠中进行的敲除研究提出了 TNFR2 通过刺激抗氧化途径保护神经元免于凋亡的作用。 TNFR2的表达可能在乳腺浸润性微乳头状癌的血管生成、肿瘤细胞增殖和转移中发挥重要作用。
有 2 种类型的可溶性 TNF 受体：sTNFR-I 和 sTNFR-II，它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。 TNFR2 和 TNFR1 形成一个异源复合物，介导 2 种抗凋亡蛋白 c-IAP1 和 c-IAP2 的募集，它们具有 E3 泛素连接酶活性。 IAP 在 TNF 受体信号传导中的功能未知；然而，据信 c-IAP1 通过介导抗凋亡信号的 TNF 受体相关因子 2 的泛素化和降解来增强 TNF 诱导的细胞凋亡。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。

TNFR2 Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 20kDa. The TNFR2 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFR2 Human 是一条单一的、非糖基化的多肽链，含有 184 个氨基酸，分子量为 20kDa。 TNFR2 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

TNFR2 was Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.4.

It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TNFR2，然后可以进一步稀释成其他水溶液。

Lyophilized TNFR2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFR2 虽然在室温下可稳定保存 3 周，但应在 -18C 以下干燥储存。 重组后 TNFR2 应在 4C 下储存 2-7 天，以备将来在 -18C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by -PAGE.

MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT.

The ED50 as determined by its ability to inhibit the TNF-a mediated cytotoxicity in the L-929 cells is less than 0.2µg/ml, corresponding to a specific activity of > 5000IU/mg in the presence of 0.25ng/mL of rHuTNF-a.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Tumor necrosis factor receptor superfamily member 1B,Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, CD120b antigen, Etanercept, TBPII, TNFBR, TNFR80, TNF-R75, p75TNFR, TNF-R-II.

TNFR binds specifically to tumor necrosis factor and blocks its interaction with cell surface TNF receptors. TNF is a naturally occurring cytokine that is involved in normal inflammatory and immune responses. It plays an important role in the inflammatory processes of rheumatoid arthritis , polyarticular-course juvenile rheumatoid arthritis , and ankylosing spondylitis and the resulting joint pathology. In addition, TNF plays a role in the inflammatory process of plaque psoriasis. Elevated levels of TNF are found in involved tissues and fluids of patients with RA, psoriatic arthritis, ankylosing spondylitis , and plaque psoriasis. Two distinct receptors for TNF , a 55 kilodalton protein and a 75 kilodalton protein , exist naturally as monomeric molecules on cell surfaces and in soluble forms. Biological activity of TNF is dependent upon binding to either cell surface TNFR. Recombinant Human TNFR is a dimeric soluble form of the p75 TNF receptor that can bind to two TNF molecules.
It inhibits the activity of TNF in vitro and has been shown to affect several animal models of inflammation, including murine collagen-induced arthritis. TNFR inhibits binding of both TNF? and TNF? to cell surface TNFRs, rendering TNF biologically inactive. Cells expressing transmembrane TNF that bind to TNFR are not lysed in vitro in the presence or absence of complement.
TNFR can also modulate biological responses that are induced or regulated by TNF, including expression of adhesion molecules responsible for leukocyte migration , serum levels of cytokines , and serum levels of matrix metalloproteinase-3 .

TNFR 特异性结合肿瘤坏死因子并阻断其与细胞表面 TNF 受体的相互作用。 TNF 是一种天然存在的细胞因子，参与正常的炎症和免疫反应。它在类风湿性关节炎、多关节病程幼年类风湿性关节炎和强直性脊柱炎的炎症过程和由此产生的关节病理中起重要作用。此外，TNF 在斑块型银屑病的炎症过程中发挥作用。在 RA、银屑病关节炎、强直性脊柱炎和斑块型银屑病患者的受累组织和体液中发现 TNF 水平升高。两种不同的 TNF 受体，一种 55 千道尔顿的蛋白质和一种 75 千道尔顿的蛋白质，以单体分子的形式自然存在于细胞表面并以可溶形式存在。 TNF 的生物学活性取决于与任一细胞表面 TNFR 的结合。重组人 TNFR 是 p75 TNF 受体的二聚体可溶形式，可与两个 TNF 分子结合。
它在体外抑制 TNF 的活性，并已显示影响多种炎症动物模型，包括小鼠胶原蛋白诱导的关节炎。 TNFR 抑制两种 TNF 的结合？和肿瘤坏死因子？到细胞表面的 TNFR，使 TNF 失去生物活性。在存在或不存在补体的情况下，表达与 TNFR 结合的跨膜 TNF 的细胞不会在体外裂解。
TNFR 还可以调节由 TNF 诱导或调节的生物反应，包括负责白细胞迁移的粘附分子的表达、细胞因子的血清水平和基质金属蛋白酶-3 的血清水平。

Recombinant Human Tumor Necrosis Factor Receptor 2 Fusion Protein produced in CHO is a dimeric, glycosylated, polypeptide chain consisting of the extracellular ligand-binding portion of the human 75 kilo Dalton tumor necrosis factor receptor 2 linked to the Fc portion of human IgG1. The Fc component of TNFR2 contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It consists of 934 amino acids and has an apparent molecular weight of approximately 150 kilo Daltons.
The TNFR2 is purified by standard chromatographic techniques.

在 CHO 中产生的重组人肿瘤坏死因子受体 2 融合蛋白是一种二聚体、糖基化的多肽链，由与人 IgG1 的 Fc 部分连接的人 75 千道尔顿肿瘤坏死因子受体 2 的细胞外配体结合部分组成。 TNFR2 的 Fc 成分包含 CH2 结构域、CH3 结构域和铰链区，但不包含 IgG1 的 CH1 结构域。 它由 934 个氨基酸组成，表观分子量约为 150 千道尔顿。
TNFR2通过标准色谱技术纯化。

Chinese Hamster Ovarian Cells .

Sterile Filtered White lyophilized powder.

Each mg contains 1.6mg mannitol, 0.4 mg sucrose and 48 µg tromethamine.

It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized Tumor Necrosis Factor Receptor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFR2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的肿瘤坏死因子受体虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TNFR2 应在 4°C 下储存 2-7 天，以备将来在低于 -18°C 的温度下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Potency is determined by its ability to neutralize TNF-alpha mediated growth inhibition of A375 cells, corresponding to a Specific Activity of 17,000,000 IU/mg.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, CD120b, Etanercept, TNF-R2, TNF-RII, TNFR-II, TNFRSF1B, TNFBR, TNFR2, TBPII, TNFR2, TNFR1B, TNFR80, TNF-R75, p75TNFR, TNF-R-II.

TNFR2 belongs to the TNF-receptor superfamily. TNFR2 is receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. TNFR2 mediates the majority of the metabolic effects of TNF-alpha. In addition, knockout studies in mice propose a role for TNFR2 in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2 expression might have a significant role in the angiogenesis, tumor cell proliferation and metastasis of Invasive micropapillary carcinoma of the breast.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women. TNFR2 and TNFR1 form a heterocomplex which mediates the recruitment of 2 anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. IAPs’ function in TNF-receptor signaling is unknown; nevertheless, c-IAP1 is believed to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.

TNFR2 属于 TNF 受体超家族。 TNFR2 是对 TNFSF2/TNF-α 具有高亲和力的受体，对同源三聚体 TNFSF1/淋巴毒素-α 的亲和力低约 5 倍。 TNFR2 介导 TNF-α 的大部分代谢作用。此外，在小鼠中进行的敲除研究提出了 TNFR2 通过刺激抗氧化途径保护神经元免于凋亡的作用。 TNFR2的表达可能在乳腺浸润性微乳头状癌的血管生成、肿瘤细胞增殖和转移中发挥重要作用。
有 2 种类型的可溶性 TNF 受体：sTNFR-I 和 sTNFR-II，它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。 TNFR2 和 TNFR1 形成一个异源复合物，介导 2 种抗凋亡蛋白 c-IAP1 和 c-IAP2 的募集，它们具有 E3 泛素连接酶活性。 IAP 在 TNF 受体信号传导中的功能未知；然而，据信 c-IAP1 通过介导抗凋亡信号的 TNF 受体相关因子 2 的泛素化和降解来增强 TNF 诱导的细胞凋亡。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。

TNFR2 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 184 amino acids fragment having a molecular weight of 24.45kDa and fused with a 4.5kDa amino-terminal hexahistidine tag.
The TNFR2 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFR2 Human Recombinant 是一个单一的、非糖基化的多肽链，包含 184 个氨基酸片段，分子量为 24.45kDa，并与 4.5kDa 氨基末端的六组氨酸标签融合。
TNFR2 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered clear solution.

TNFR2 protein is supplied in 20mM Tris HCl pH-8, 5mM EDTA and 50% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
请避免冻融循环。

Greater than 95.0% as determined by -PAGE.

LPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWV
PECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSD
VVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPT.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Tumor Necrosis Factor Receptor Superfamily, Member 1B, TNFR2, TNFBR, Tumor Necrosis Factor Receptor Type II, Tumor Necrosis Factor Receptor 2, P80 TNF-Alpha Receptor, TNF-RII, TNF-R2, P75, Tumor Necrosis Factor Receptor Superfamily Member 1B, Tumor Necrosis Factor Binding Protein 2, Tumor Necrosis Factor Beta Receptor, Soluble TNFR1B Variant 1, P75 TNF Receptor, CD120b Antigen, Etanercept, TNF-R-II, TNF-R75, P75TNFR, TNFR-II, CD120b, TNFR1B, TNFR80, TBPII.

Sf9, Baculovirus cells.

Sterile filtered colorless solution.

TNFR2 protein solution contains Phosphate buffered saline and 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Greater than 95.0% as determined by -PAGE.

LPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPTRSMAPG AVHLPQPVST RSQHTQPTPE PSTAPSTSFL LPMGPSPPAE GSTGDHHHHH H

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, CD120b, Tnfrsf1b, Tnfr-2, Tnfr2, TNFBR, TNFR80, TNFRII, TNF-R75, TNF-R-II, TNF-alphaR2, TNFalpha-R2.

TNFR2 belongs to the TNF-receptor superfamily. TNFR2 is receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. TNFR2 mediates the majority of the metabolic effects of TNF-alpha. In addition, knockout studies in mice propose a role for TNFR2 in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2 expression might have a significant role in the angiogenesis, tumor cell proliferation and metastasis of Invasive micropapillary carcinoma of the breast.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women. TNFR2 and TNFR1 form a heterocomplex which mediates the recruitment of 2 anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. IAPs’ function in TNF-receptor signaling is unknown; nevertheless, c-IAP1 is believed to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.

TNFR2 属于 TNF 受体超家族。 TNFR2 是对 TNFSF2/TNF-α 具有高亲和力的受体，对同源三聚体 TNFSF1/淋巴毒素-α 的亲和力低约 5 倍。 TNFR2 介导 TNF-α 的大部分代谢作用。此外，在小鼠中进行的敲除研究提出了 TNFR2 通过刺激抗氧化途径保护神经元免于凋亡的作用。 TNFR2的表达可能在乳腺浸润性微乳头状癌的血管生成、肿瘤细胞增殖和转移中发挥重要作用。
有 2 种类型的可溶性 TNF 受体：sTNFR-I 和 sTNFR-II，它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。 TNFR2 和 TNFR1 形成一个异源复合物，介导 2 种抗凋亡蛋白 c-IAP1 和 c-IAP2 的募集，它们具有 E3 泛素连接酶活性。 IAP 在 TNF 受体信号传导中的功能未知；然而，据信 c-IAP1 通过介导抗凋亡信号的 TNF 受体相关因子 2 的泛素化和降解来增强 TNF 诱导的细胞凋亡。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。

TNFR2 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 236 amino acids and having a molecular mass of 25.3kDa.
The TNFR2 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFR2 Mouse Recombinant 是单条非糖基化多肽链，含有 236 个氨基酸，分子量为 25.3kDa。
TNFR2 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

TNFR2 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TNFR2，然后可以进一步稀释成其他水溶液。

Lyophilized TNFR2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFR2 虽然在室温下可稳定保存 3 周，但应在 -18C 以下干燥储存。 重组后 TNFR2 应在 4C 下储存 2-7 天，以备将来在 -18C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by -PAGE.

VPAQVVLTPY KPEPGYECQI SQEYYDRKAQ MCCAKCPPGQ YVKHFCNKTS DTVCADCEAS MYTQVWNQFR TCLSCSSSCT TDQVEIRACT KQQNRVCACE AGRYCALKTH SGSCRQCMRL SKCGPGFGVA SSRAPNGNVL CKACAPGTFS DTTSSTDVCR PHRICSILAI PGNASTDAVC APESPTLSAI PRTLYVSQPE PTRSQPLDQE PGPSQTPSIL TSLGSTPIIE QSTKGG.

The ED₅₀ as determined by its ability to inhibit the TNF-a mediated cytotoxicity in the L-929 cells is less than 2µg/ml, corresponding to a specific activity of > 500IU/mg in the presence of 0.1ng/mL of rHuTNF-a.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Tumor Necrosis Factor Receptor Superfamily Member 10a, TRAILR1, APO2, DR4, TNF-Related Apoptosis-Inducing Ligand Receptor 1, Death Receptor 4, TRAIL Receptor 1, TRAIL-R1, Tumor Necrosis Factor Receptor Superfamily Member 10a Variant 2, Tumor Necrosis Factor Receptor Superfamily Member 10A, Cytotoxic TRAIL Receptor, CD261 Antigen, TRAILR-1, CD261, TNFRSF10A.

Human TNFRSF10A is a type 1 transmembrane protein in the TNF R superfamily. TNFRSF10A is not expressed in rodents. The trimeric ligand Trail binds TNFRSF10A and induces apoptosis, and recombinant, soluble forms of the receptor inhibit Trail-induced apoptosis. TNFRSF10A is expressed generally in damaged, infected, and malignant cells. TNFRSF10A functions in immune surveillance, inducing apoptosis in cancer cells but not normal cells.

人 TNFRSF10A 是 TNF R 超家族中的 1 型跨膜蛋白。 TNFRSF10A 在啮齿动物中不表达。 三聚体配体 Trail 结合 TNFRSF10A 并诱导细胞凋亡，而重组的可溶性受体形式可抑制 Trail 诱导的细胞凋亡。 TNFRSF10A 通常在受损、感染和恶性细胞中表达。 TNFRSF10A 在免疫监视中起作用，诱导癌细胞而非正常细胞的凋亡。

TRAIL R1 Fc Human Recombinant produced in E.Coli is a non-glycosylated, disulfide-linked homodimer containing two polypeptide chains of 217 amino acids each and having a molecular mass of 41kDa [including tag] each chain.
TRAILR1 is fused to a 236 amino acid Fc tag at C-terminal and purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TRAIL R1 Fc 人重组体是一种非糖基化、二硫键连接的同源二聚体，含有两条多肽链，每条 217 个氨基酸，每条链的分子量为 41kDa [包括标签]。
TRAILR1 在 C 末端与 236 个氨基酸的 Fc 标签融合，并通过专有色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a 0.2µm filtered solution in PBS.

It is recommended to reconstitute the lyophilized TNFRSF10A in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized TNFRSF10A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TRAIL-R1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFRSF10A 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TRAIL-R1 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as determined by -PAGE.

ASGTEAAAAT PSKVWGSSAG RIEPRGGGRG ALPTSMGQHG PSARARAGRA PGPRPAREAS PRLRVHKTFK FVVVGVLLQV VPSSAATIKL HDQSIGTQQW EHSPLGELCP PGSHRSERPG ACNRCTEGVG YTNASNNLFA CLPCTACKSD EEERSPCTTT RNTACQCKPG TFRNDNSAEM CRKCSTGCPR GMVKVKDCTP WSDIECVHKE SGNGHNIEGR MDPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK.

The ED₅₀, as measured by its ability to inhibit Trail-mediated cytotoxicity in mouse L-929 cells, is less than 1.25ng/ml.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2, CD262, TNFRSF10B, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9, TRICKB, TRICK2A, TRICK2B, KILLER/DR5.

TRAIL Receptor-1 and TRAIL Receptor-2 are members of the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain”, which is capable of activating the cell’s apoptotic machinery. These receptors are activated by binding to either membrane anchored or soluble TRAIL/Apo2L.

TRAIL Receptor-1 和 TRAIL Receptor-2 是跨膜蛋白 TNFR 超家族的成员，含有细胞质“死亡结构域”，能够激活细胞的凋亡机制。 这些受体通过与膜锚定或可溶性 TRAIL/Apo2L 结合而被激活。

TNFRSF10B Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 132 amino acids and having a molecular mass of 14.8kDa.
The TNFRSF10B is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFRSF10B 人重组体是一种单一的、非糖基化的多肽链，含有 132 个氨基酸，分子量为 14.8kDa。
TNFRSF10B 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.4.

It is recommended to reconstitute the lyophilized TNFRSF10B in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的 TNFRSF10B 溶解在不低于 100µg/ml 的无菌 18M-cm H2O 中，然后可以进一步稀释成其他水溶液。

Lyophilized TNFRSF10B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFRSF10B should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFRSF10B 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TNFRSF10B 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as determined by -PAGE.

ESALITQQD LAPQQRVAPQ QKRSSPSEGL CPPGHHISED GRDCISCKYG QDYSTHWNDL LFCLRCTRCD SGEVELSPCT TTRNTVCQCE EGTFREEDSP EMCRKCRTGC PRGMVKVGDC TPWSDIECVH KES.

The TNFRSF10B reduced the production of LPS-induced TNF by its ability to neutralize endogenous TRAIL in fresh human PBMC. In this assay, endogenous TRAIL is induced during a 24 hour exposure to LPS but in the presence of TNFRSF10B, TRAIL-induced TNF is suppressed.

TNFRSF10B 通过中和新鲜人 PBMC 中内源性 TRAIL 的能力来减少 LPS 诱导的 TNF 的产生。 在该试验中，内源性 TRAIL 在 24 小时暴露于 LPS 期间被诱导，但在 TNFRSF10B 存在下，TRAIL 诱导的 TNF 被抑制。

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

TNFRSF10B, CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2.

TRAIL Receptor-1 and TRAIL Receptor-2 are members of the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain”, which is capable of activating the cell’s apoptotic machinery. These receptors are activated by binding to either membrane anchored or soluble TRAIL/Apo2L.

TRAIL Receptor-1 和 TRAIL Receptor-2 是跨膜蛋白 TNFR 超家族的成员，含有细胞质“死亡结构域”，能够激活细胞的凋亡机制。 这些受体通过与膜锚定或可溶性 TRAIL/Apo2L 结合而被激活。

TNFRSF10B produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 394 amino acids and having a molecular mass of 43.9kDa. .
TNFRSF10B is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFRSF10B 是单条糖基化多肽链，包含 394 个氨基酸，分子量为 43.9kDa。 .
TNFRSF10B 在 C 末端带有 239 个氨基酸的 hIgG-His 标签，并通过专有色谱技术进行纯化。

Sf9, Insect cells.

Sterile Filtered colorless solution.

TNFRSF10B protein solution contains Phosphate Buffered Saline and 10% glycerol.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。 对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Greater than 90% as determined by -PAGE.

Measured by its ability to inhibit cytotoxicity assay using Jurkat human T lymphocyte. The ED50 for this effect ≤ to 5ng/ml with TRAIL

使用 Jurkat 人 T 淋巴细胞通过其抑制细胞毒性测定的能力来测量。 此效果的 ED50 ≤ 至 5ng/ml 与 TRAIL

.

ITQQDLAPQQ RAAPQQKRSS PSEGLCPPGH HISEDGRDCI SCKYGQDYST HWNDLLFCLR CTRCDSGEVE LSPCTTTRNT VCQCEEGTFR EEDSPEMCRK CRTGCPRGMV KVGDCTPWSD IECVHKESGT KHSGEVPAVE ETVTSSPGTP ASPCSLEPKS CDKTHTCPPC PAPELLGGPS
VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ
GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。