分类： 趋化因子Chemokines

ProSpec,Rantes Rhesus Macaque,Rantes恒河猴重组体CHM-033

ProSpec,Rantes Rhesus Macaque,Rantes恒河猴重组体CHM-033,冻干的 Rantes 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CCL5 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

  • Rantes Rhesus Macaque

  • Rantes Rhesus Macaque Recombinant
  • CHM-033

Catalogue number

CHM-033

Synonyms

C-C motif chemokine 5, Small-inducible cytokine A5, T-cell-specific protein RANTES, CCL5.

Introduction

Regulated upon Activation, Normal T-cell Expressed, and Secreted or RANTES is an 8 kDa protein classified as a chemotactic cytokine or chemokine. It has recently been renamed CCL5. RANTES is chemotactic for T cells, eosinophils and basophils and plays an active role in recruiting leukocytes into inflammatory sites. With the help of particular cytokines that are released by T cells, RANTES also induces the proliferation and activation of certain natural killer cells to form CHAK cells. It is also a HIV-suppressive factor released from CD8+ T cells. This chemokine has been localized to chromosome 17 in humans.
受激活、正常 T 细胞表达和分泌或 RANTES 的调节是一种 8 kDa 蛋白质，归类为趋化细胞因子或趋化因子。 它最近已更名为 CCL5。 RANTES 对 T 细胞、嗜酸性粒细胞和嗜碱性粒细胞具有趋化作用，并在将白细胞募集到炎症部位方面发挥积极作用。 在 T 细胞释放的特定细胞因子的帮助下，RANTES 还诱导某些自然杀伤细胞的增殖和活化，形成 CHAK 细胞。 它也是一种从 CD8+ T 细胞释放的 HIV 抑制因子。 这种趋化因子已定位于人类的第 17 号染色体。

Description

Rantes Rhesus Macaque Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 68 amino acids and having a molecular mass of 7.8kDa.
The CCL5 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Rantes protein was lyophilized from a 0.2µm filtered concentrated solution in 20mM PB pH 6.0 and 500mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized Rantes in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Rantes although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL5 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 Rantes 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CCL5 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

SPHASDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS.

Biological Activity

The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prospec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec,SDF 1a Human,基质细胞衍生因子 1 Alpha 人类重组CHM-262

CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。ProSpec,SDF 1a Human,基质细胞衍生因子 1 Alpha 人类重组CHM-262

  • SDF 1a Human

  • Stromal Cell-Derived Factor-1 Alpha Human Recombinant
  • CHM-262

Catalogue number

CHM-262

Synonyms

SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a.

Introduction

SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。
CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。

Description

Stromal Cell-Derived Factor-1 alpha Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 8004 Dalton.
The SDF-1a is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a concentrated sterile solution containing no additives.

Solubility

It is recommended to reconstitute the lyophilized Stromal Cell-Derived Factor-1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized SDF-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Biological Activity

The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80 ng/ml corresponding to a Specific Activity of 12,500-50,000IU/mg.

References

1.Title: Stromal Cell-Derived Factor-1a Promotes Neuroprotection, Angiogenesis, and Mobilization/Homing of Bone Marrow- Derived Cells in Stroke Rats.

Publication: THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS Copyright © 2008 by The American Society for Pharmacology and Experimental Therapeutics JPET 324:834–849, 2008. Vol. 324, No. 2 127746/3301181 ed in U.S.A.
Link: http://jpet.aspetjournals.org/content/324/2/834.full.pdf

 

Application: Intracerebral administration of SDF-1 resulted in neuroprotection against neurotoxic insult, and it induced increased BM-derived cell targeting to the ischemic brain, thereby reducing the volume of cerebral infarction and improving neural plasticity.

2.Title:MECHANISMS OF BIOMATERIAL MEDIATED FIBROTIC RESPONSES AND STRATEGIES TO
IMPROVE TISSUE REACTIONS TO BIOMATERIAL IMPLANTS.
Publication:THE UNIVERSITY OF TEXAS AT ARLINGTON
May 2010
Link:http://dspace.uta.edu/bitstream/handle/10106/4918/Thevenot_uta_2502D_10646.pdf?sequence=1

 

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-SDF 1a Human His,基质细胞衍生因子 1 α 人类重组CHM-241

这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。ProSpec-SDF 1a Human His,基质细胞衍生因子 1 α 人类重组CHM-241

  • SDF 1a Human, His

  • Stromal Cell-Derived Factor-1 alpha Human Recombinant, His Tag
  • CHM-241

Catalogue number

CHM-241

Synonyms

SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a.

Introduction

SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。
CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。

Description

Stromal Cell-Derived Factor-1 alpha Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 78 amino acids, having a molecular mass of 9.2 kDa.
The SDF-1a is fused to 10 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

White lyophilized powder.

Formulation

The protein was filtered and lyophilized from a concentrated solution containing 20mM Tris buffer pH-7.5 and 20mM sodium chloride.

Solubility

It is recommended to add deionized water to a working concentration approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.

Stability

Lyophilized SDF1A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined -PAGE.

Amino acid sequence

MKHHHHHHAS KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec,SDF 1a Mouse,基质细胞衍生因子 1 α 小鼠重组CHM-324

ProSpec,SDF 1a Mouse,基质细胞衍生因子 1 α 小鼠重组CHM-324,CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。

  • SDF 1a Mouse

  • Stromal Cell-Derived Factor-1 Alpha Mouse Recombinant
  • CHM-324

Catalogue number

CHM-324

Synonyms

SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell-stimulating factor, TLSF.

Introduction

SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。
CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。

Description

Stromal Cell-Derived Factor-1 alpha Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 8 kDa.

The SDF-1a is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid .

Solubility

It is recommended to reconstitute the lyophilized Stromal Cell-Derived Factor-1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized SDF-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 SDF-1a 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CXCL12 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu.

Biological Activity

The specific activity as determined by its ability to chemoattract human peripheral blood monocytes at 50-100 ng/ml corresponding to a Specific Activity of 10,000-20,000IU/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-SDF 1a Mouse His,基质细胞衍生因子-1α,小鼠重组CHM-323

ProSpec-SDF 1a Mouse His,基质细胞衍生因子-1α,小鼠重组CHM-323,这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。

  • SDF 1a Mouse, His

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  • CHM-323

Catalogue number

CHM-323

Synonyms

SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a, 12-O tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell stimulating factor, TLSF.

Introduction

SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。
CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。

Description

SDF 1a Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 91 amino acids and having a molecular mass of 10.4kDa. SDF 1a is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

SDF 1a protein solution containing Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSKPVSLSY RCPCRFFESH IARANVKHLK ILNTPNCALQ IVARLKNNNR QVCIDPKLKW IQEYLEKALN K.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec,SDF 1a Rat,基质细胞衍生因子 1 α 大鼠重组CHM-354

SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b,ProSpec,SDF 1a Rat,基质细胞衍生因子 1 α 大鼠重组CHM-354

  • SDF 1a Rat

  • Stromal Cell-Derived Factor-1 alpha Rat Recombinant
  • CHM-354

Catalogue number

CHM-354

Synonyms

SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell-stimulating factor, TLSF.

Introduction

SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。
CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。

Description

Stromal Cell-Derived Factor-1 alpha Rat Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 7.9 kDa.
The SDF-1a is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The CXCL12 protein was lyophilized from a concentrated sterile solution containing 20mM Phosphate buffer pH-7.4 and 150mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized SDF-1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized SDF-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK.

Biological Activity

The specific activity as determined by its ability to chemoattract human peripheral blood monocytes at 50-100ng/ml corresponding to a Specific Activity of 10,000-20,000IU/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • SDF 1a Mouse
  • SDF 1b Mouse
  • SDF 1a Human
  • SDF 1a Human, His
  • SDF 1a Mouse, His
  • SDF 1b Human, His
  • SDF 1b Human
  • SDF 1b Rat
  • SDF 1b Feline

ProSpec,SDF 1b Feline,基质细胞衍生因子 1 β 猫重组CHM-247

ProSpec,SDF 1b Feline,基质细胞衍生因子 1 β 猫重组CHM-247,冻干的 SDF-1b 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CXCL12 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

  • SDF 1b Feline

  • Stromal Cell-Derived Factor-1 beta Feline Recombinant
  • CHM-247

Catalogue number

CHM-247

Synonyms

SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine ligand 12, SDF1, SDF1B, TPAR1, SCYB12, SDF-1b, TLSF-b, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell-stimulating factor, TLSF.

Introduction

SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。
CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。

Description

Stromal Cell-Derived Factor-1 beta Feline Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8526 Dalton.
The SDF-1b is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的基质细胞衍生因子 1 β 猫重组物是一种非糖基化的多肽链，含有 72 个氨基酸，分子量为 8526 道尔顿。
SDF-1b 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a concentrated sterile solution containing no additives.

Solubility

It is recommended to reconstitute the lyophilized SDF-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized SDF-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 SDF-1b 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CXCL12 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRF KM.

Biological Activity

The specific activity as determined by its ability to chemoattract human peripheral T cells stimulated with IL-2 and PHA in a concentration range from 10-75 ng/ml corresponding to a Specific Activity of 13,334-100,000IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • SDF 1a Mouse
  • SDF 1a Human
  • SDF 1b Mouse
  • SDF 1b Human, His
  • SDF 1b Rat
  • SDF 1a Human, His
  • SDF 1a Rat
  • SDF 1b Human

ProSpec,SDF 1b Human,基质细胞衍生因子 1 Beta 人类重组CHM-325

ProSpec,SDF 1b Human,基质细胞衍生因子 1 Beta 人类重组CHM-325,SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。

  • SDF 1b Human

  • Stromal Cell Derived Factor-1 Beta Human Recombinant
  • CHM-325

Catalogue number

CHM-325

Synonyms

SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine ligand 12, SDF1, SDF1B, TPAR1, SCYB12, SDF-1b, TLSF-b.

Introduction

SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。
CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。

Description

Stromal Cell-Derived Factor-1 beta Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8508 Dalton.
The SDF-1b is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The CXCL12 protein was lyophilized from a concentrated sterile solution containing no additives.

Solubility

It is recommended to reconstitute the lyophilized Stromal Cell-Derived Factor-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized SDF-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 SDF-1b 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CXCL12 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu.

Biological Activity

The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80ng/ml corresponding to a Specific Activity of 12,500-50,000IU/mg.

Protein content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a standard solution of SDF-1b as a Reference Standard.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • SDF 1b Human, His
  • SDF 1a Rat
  • SDF 1b Mouse
  • SDF 1b Rat
  • SDF 1b Feline
  • SDF 1a Human, His
  • SDF 1a Mouse
  • SDF 1a Human

ProSpec-SDF 1b Human His,基质细胞衍生因子 1 β 人类重组CHM-249

ProSpec-SDF 1b Human His,基质细胞衍生因子 1 β 人类重组CHM-249,CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。

  • SDF 1b Human, His

  • Stromal Cell-Derived Factor-1 beta Human Recombinant , His Tag
  • CHM-249

Catalogue number

CHM-249

Synonyms

SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine ligand 12, SDF1, SDF1B, TPAR1, SCYB12, SDF-1b, TLSF-b, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell-stimulating factor, TLSF.

Introduction

SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor. The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。 CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。

Description

SDF-1 beta Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 93 amino acids and having a molecular mass of 10.8 KDa.
The SDF-1b is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

The SDF-1 beta His Tag protein contains 20mM Tris-HCl buffer pH-8 and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90% as determined by Analysis by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKR FKM.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • SDF 1b Rat
  • SDF 1a Mouse
  • SDF 1b Mouse
  • SDF 1a Human
  • SDF 1a Human, His
  • SDF 1b Feline
  • SDF 1a Rat
  • SDF 1b Human

ProSpec,SDF 1b Mouse,基质细胞衍生因子-1 β 小鼠重组CHM-326

ProSpec,SDF 1b Mouse,基质细胞衍生因子-1 β 小鼠重组CHM-326,CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。

  • SDF 1b Mouse

  • Stromal Cell Derived Factor-1 Beta Mouse Recombinant
  • CHM-326

Catalogue number

CHM-326

Synonyms

SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine ligand 12, SDF1, SDF1B, TPAR1, SCYB12, SDF-1b, TLSF-b, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell-stimulating factor, TLSF.

Introduction

SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。
CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。

Description

Stromal Cell-Derived Factor-1 beta Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8513 Dalton.
The SDF-1b is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的基质细胞衍生因子-1 β 小鼠重组体是一种非糖基化的多肽链，含有 72 个氨基酸，分子量为 8513 道尔顿。
SDF-1b 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The CXCL12 protein was lyophilized from a concentrated sterile solution containing no additives.

Solubility

It is recommended to reconstitute the lyophilized Stromal Cell-Derived Factor-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized SDF-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu.

Biological Activity

The specific activity as determined by its ability to chemoattract human monocytes at 50-100ng/ml corresponding to a Specific Activity of 10,000-20,000IU/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • SDF 1a Human
  • SDF 1b Human
  • SDF 1a Rat
  • SDF 1b Human, His
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  • SDF 1a Human, His
  • SDF 1b Rat
  • SDF 1a Mouse

ProSpec,SDF 1b Rat,基质细胞衍生因子 1 β 大鼠重组CHM-248

ProSpec,SDF 1b Rat,基质细胞衍生因子 1 β 大鼠重组CHM-248,这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。

  • SDF 1b Rat

  • Stromal Cell-Derived Factor-1 beta Rat Recombinant
  • CHM-248

Catalogue number

CHM-248

Synonyms

SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine ligand 12, SDF1, SDF1B, TPAR1, SCYB12, SDF-1b, TLSF-b, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell-stimulating factor, TLSF.

Introduction

SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。
CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。

Description

SDF-1 beta Rat Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids and having a molecular mass of 8.4 kDa.
The Rat SDF-1b is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The Rat SDF1B protein was lyophilized from a concentrated sterile solution containing 20mM Phsophate buffer pH-7.4 and 0.15M NaCl.

Solubility

It is recommended to reconstitute the lyophilized SDF-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized SDF-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 SDF-1b 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CXCL12 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNKRL KM.

Biological Activity

The specific activity as determined by its ability to chemoattract human peripheral blood monocytes at 100-150ng/ml corresponding to a Specific Activity of 6,667-10,000IU/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • SDF 1a Mouse
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  • SDF 1b Human
  • SDF 1b Feline

ProSpec,SDF 1g Human,基质细胞衍生因子 1 γ 人重组CHM-020

ProSpec,SDF 1g Human,基质细胞衍生因子 1 γ 人重组CHM-020,CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。

  • SDF 1g Human

  • Stromal Cell-Derived Factor-1 gamma Human Recombinant
  • CHM-020

Catalogue number

CHM-020

Synonyms

Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, hIRH, Pre-B cell growth-stimulating factor, PBSF, CXCL12, SDF1, hSDF-1gamma, SDF-1g, TLSF, TPAR1, SCYB12.

Introduction

SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1a/CXCL12a and SDF-1b/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过相同基因的交替剪接以两种形式产生，SDF-1a/CXCL12a 和 SDF-1b/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。
CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。

Description

SDF-1 gamma Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 99 amino acids and having a molecular mass of 11.6kDa.
The SDF-1g is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

SDF-1g protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized SDF-1g in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized SDF-1g although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SDF-1 gamma should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 SDF-1g 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，SDF-1 gamma 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 96.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKG RREEKVGKKE KIGKKKRQKK RKAAQKRKN.

Biological Activity

The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 30-100 ng/ml.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-SDF2 Human,基质细胞衍生因子 2 人重组CHM-028

ProSpec-SDF2 Human,基质细胞衍生因子 2 人重组CHM-028,SDF2蛋白的表达无处不在，该基因在哺乳动物中相当保守。 SDF2 是一种蛋白质编码基因，其可变剪接导致编码和非编码变体。

  • SDF2 Human

  • Stromal Cell-Derived Factor 2 Human Recombinant
  • CHM-028

Catalogue number

CHM-028

Synonyms

Stromal cell-derived factor 2, SDF-2.

Introduction

Stromal Cell-Derived Factor 2 is a secretory protein which is partly similar to the hydrophilic segments of yeast mannosyltransferases. SDF2 protein’s expression is ubiquitous and the gene is rather conserved among mammals. SDF2is a protein-coding gene whose alternative splicing results in coding and non-coding variants.
基质细胞衍生因子 2 是一种分泌蛋白，部分类似于酵母甘露糖基转移酶的亲水片段。 SDF2蛋白的表达无处不在，该基因在哺乳动物中相当保守。 SDF2 是一种蛋白质编码基因，其可变剪接导致编码和非编码变体。

Description

SDF2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 216 amino acids and having a molecular mass of 23.7kDa.
SDF2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 SDF2 Human Recombinant 是单条非糖基化多肽链，包含 216 个氨基酸，分子量为 23.7kDa。
SDF2 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

SDF2 protein solution containing 20mM Tris-HCl buffer , 10% glycerol and 0.4M Urea.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSSSLGVVT CGSVVKLLNT RHNVRLHSHD VRYGSGSGQQ SVTGVTSVDD SNSYWRIRGK SATVCERGTP IKCGQPIRLT HVNTGRNLHS HHFTSPLSGN QEVSAFGEEG EGDYLDDWTV LCNGPYWVRD GEVRFKHSST EVLLSVTGEQ YGRPISGQKE VHGMAQPSQN NYWKAMEGIF MKPSELLKAE AHHAEL.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • SDF2 Human, sf9

ProSpec,SDF2 Human sf9,基质细胞衍生因子2 Sf9 人重组CHM-034

ProSpec,SDF2 Human sf9,基质细胞衍生因子2 Sf9 人重组CHM-034,SDF2 是一种蛋白质编码基因，其可变剪接导致编码和非编码变体。

  • SDF2 Human, sf9

  • Stromal Cell-Derived Factor 2, Sf9 Human Recombinant
  • CHM-034

Catalogue number

CHM-034

Synonyms

Stromal Cell Derived Factor 2, Stromal Cell-Derived Factor 2, SDF-2

Introduction

Stromal Cell-Derived Factor 2 is a secretory protein which is partly similar to the hydrophilic segments of yeast mannosyltransferases. SDF2 protein’s expression is ubiquitous and the gene is rather conserved among mammals. SDF2 is a protein-coding gene whose alternative splicing results in coding and non-coding variants.

基质细胞衍生因子 2 是一种分泌蛋白，部分类似于酵母甘露糖基转移酶的亲水片段。 SDF2蛋白的表达无处不在，该基因在哺乳动物中相当保守。 SDF2 是一种蛋白质编码基因，其可变剪接导致编码和非编码变体。

Description

SDF2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 202 amino acids and having a molecular mass of 22.3kDa. . SDF2 is expressed with an 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 SDF2 是单条糖基化多肽链，包含 202 个氨基酸，分子量为 22.3kDa。 . SDF2 在 C 末端用 9 个氨基酸 His 标签表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

SDF2 protein solution contains 50mM Tris-HCl , 10% glycerol, 0.1M NaCl 0.1mM PMSF and 0.5mM EDTA.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

ADPSSLGVVT CGSVVKLLNT RHNVRLHSHD VRYGSGSGQQ SVTGVTSVDD SNSYWRIRGK SATVCERGTP IKCGQPIRLT HVNTGRNLHS HHFTSPLSGN QEVSAFGEEG EGDYLDDWTV LCNGPYWVRD GEVRFKHSST EVLLSVTGEQ YGRPISGQKE VHGMAQPSQN NYWKAMEGIF MKPSELLKAE AHHAELHHHH HH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • SDF2 Human

ProSpec,TARC Human,胸腺和活化调节的趋化因子人重组CHM-240

ProSpec,TARC Human,胸腺和活化调节的趋化因子人重组CHM-240,TARC cDNA 编码一个 94 个氨基酸的前体蛋白和一个 23 个氨基酸残基的信号肽，该信号肽被切割掉以产生 71 个氨基酸残基的成熟分泌蛋白。

  • TARC Human

  • Thymus & Activation Regulated Chemokine Human Recombinant
  • CHM-240

Catalogue number

CHM-240

Synonyms

C-C motif chemokine 17, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, CC chemokine TARC, ABCD-2, CCL17, CCL-17, SCYA17, TARC, A-152E5.3, MGC138271, MGC138273.

Introduction

TARC cDNA encodes a 94 amino acid precursor protein with a 23 amino acid residue signal peptide that is cleaved off to generate the 71 amino acid residue mature secreted protein. Along with CC chemokine family members, CCL-17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1a, MIP-1b, MCP-1, MCP-2, MCP-3 and I-309. TARC is expressed in thymus, and at a lower level in the lung, colon, and small intestine. TARC is in addition transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant TARC has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL-17 was recently identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells. CCL17 is one of quite a few Cys-Cys cytokine genes clustered on the q arm of chromosome 16. CCL17 shows chemotactic activity for T lymphocytes, but not monocytes or granulocytes. CCL17 binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells.
TARC cDNA 编码一个 94 个氨基酸的前体蛋白和一个 23 个氨基酸残基的信号肽，该信号肽被切割掉以产生 71 个氨基酸残基的成熟分泌蛋白。与 CC 趋化因子家族成员一起，CCL-17 与 RANTES、MIP-1a、MIP-1b、MCP-1、MCP-2、MCP-3 和 I-309 具有大约 24-29% 的氨基酸序列同一性。 TARC 在胸腺中表达，在肺、结肠和小肠中表达水平较低。此外，TARC 在受刺激的外周血单核细胞中瞬时表达。重组 TARC 已被证明对 T 细胞系具有趋化性，但对单核细胞或中性粒细胞没有。 CCL-17 最近被确定为 CCR4 的特异性功能配体，CCR4 是一种选择性表达于 T 细胞上的受体。 CCL17 是聚集在 16 号染色体 q 臂上的大量 Cys-Cys 细胞因子基因之一。CCL17 对 T 淋巴细胞显示趋化活性，但对单核细胞或粒细胞没有趋化活性。 CCL17 与趋化因子受体 CCR4 和 CCR8 结合。这种趋化因子在胸腺 T 细胞发育以及成熟 T 细胞的运输和激活中起重要作用。

Description

CCL17 Human Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 8 kDa.
The TARC is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a concentrated solution containing 20mM PBS & 150mM NaCl pH-7.4.

Solubility

It is recommended to reconstitute the lyophilized CCL17 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TARC although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TARC should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Biological Activity

Determined by its ability to chemoattract human T-Lymphocytes using a concentration range of 1.0-10.0 ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

ARGTNVGRECCLEYFKGAIPLRKLKTWYQTSEDCSRD
AIVFVTVQGRAICSDPNNK RVKNAVKYLQSLERS.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TARC Mouse
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  • TARC Human, His
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ProSpec-TARC Human His,胸腺和活化调节的趋化因子人重组CHM-359

CCL-17 最近被确定为 CCR4 的特异性功能配体，CCR4 是一种选择性表达于 T 细胞上的受体。 CCL17 是聚集在 16 号染色体 q 臂上的大量 Cys-Cys 细胞因子基因之一。ProSpec-TARC Human His,胸腺和活化调节的趋化因子人重组CHM-359

  • TARC Human, His

  • Thymus and Activation Regulated Chemokine Human Recombinant , His Tag
  • CHM-359

Catalogue number

CHM-359

Synonyms

C-C motif chemokine 17, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, CC chemokine TARC, ABCD-2, CCL17, CCL-17, SCYA17, TARC, A-152E5.3, MGC138271, MGC138273.

Introduction

TARC cDNA encodes a 94 amino acid precursor protein with a 23 amino acid residue signal peptide that is cleaved off to generate the 71 amino acid residue mature secreted protein. Along with CC chemokine family members, CCL-17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1a, MIP-1b, MCP-1, MCP-2, MCP-3 and I-309. TARC is expressed in thymus, and at a lower level in the lung, colon, and small intestine. TARC is in addition transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant TARC has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL-17 was recently identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells. CCL17 is one of quite a few Cys-Cys cytokine genes clustered on the q arm of chromosome 16. CCL17 shows chemotactic activity for T lymphocytes, but not monocytes or granulocytes. CCL17 binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells.
TARC cDNA 编码一个 94 个氨基酸的前体蛋白和一个 23 个氨基酸残基的信号肽，该信号肽被切割掉以产生 71 个氨基酸残基的成熟分泌蛋白。与 CC 趋化因子家族成员一起，CCL-17 与 RANTES、MIP-1a、MIP-1b、MCP-1、MCP-2、MCP-3 和 I-309 具有大约 24-29% 的氨基酸序列同一性。 TARC 在胸腺中表达，在肺、结肠和小肠中表达水平较低。此外，TARC 在受刺激的外周血单核细胞中瞬时表达。重组 TARC 已被证明对 T 细胞系具有趋化性，但对单核细胞或中性粒细胞没有。 CCL-17 最近被确定为 CCR4 的特异性功能配体，CCR4 是一种选择性表达于 T 细胞上的受体。 CCL17 是聚集在 16 号染色体 q 臂上的大量 Cys-Cys 细胞因子基因之一。CCL17 对 T 淋巴细胞显示趋化活性，但对单核细胞或粒细胞没有趋化活性。 CCL17 与趋化因子受体 CCR4 和 CCR8 结合。这种趋化因子在胸腺 T 细胞发育以及成熟 T 细胞的运输和激活中起重要作用。

Description

TARC Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 92 amino acids and having a molecular mass of 10.3 kDa.
The TARC is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TARC 人重组体是一种非糖基化的多肽链，含有 92 个氨基酸，分子量为 10.3 kDa。
TARC 在 N 末端与 20 个氨基酸 His-Tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

The TARC protein contains 1xPBS and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95% as determined by Analysis by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MARGTNVGRE CCLEYFKGAI PLRKLKTWYQ TSEDCSRDAI VFVTVQGRAI CSDPNNKRVK NAVKYLQSLE RS.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TARC Mouse
  • TARC Rat
  • TARC Human
  • TARC Macaque

ProSpec,TARC Macaque,胸腺和活化调节的趋化因子恒河猴重组CHM-036

TARC 在胸腺中表达，在肺、结肠和小肠中表达水平较低。此外，TARC 在受刺激的外周血单核细胞中瞬时表达。重组 TARC 已被证明对 T 细胞系具有趋化性，但对单核细胞或中性粒细胞没有。

  • TARC Macaque

  • Thymus and Activation Regulated Chemokine Rhesus Macaque Recombinant
  • CHM-036

Catalogue number

CHM-036

Synonyms

C-C motif chemokine 17, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, CC chemokine TARC, ABCD-2, CCL17, CCL-17, SCYA17, TARC, A-152E5.3, MGC138271, MGC138273.

Introduction

TARC cDNA encodes a 94 amino acid precursor protein with a 23 amino acid residue signal peptide that is cleaved off to generate the 71 amino acid residue mature secreted protein. Along with CC chemokine family members, CCL-17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1a, MIP-1b, MCP-1, MCP-2, MCP-3 and I-309. TARC is expressed in thymus, and at a lower level in the lung, colon, and small intestine. TARC is in addition transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant TARC has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL-17 is a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells. CCL17 is one of a few Cys-Cys cytokine genes clustered on the q arm of chromosome 16. CCL17 shows chemotactic activity for T lymphocytes, but not monocytes or granulocytes. CCL17 binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells.

TARC cDNA 编码一个 94 个氨基酸的前体蛋白和一个 23 个氨基酸残基的信号肽，该信号肽被切割掉以产生 71 个氨基酸残基的成熟分泌蛋白。与 CC 趋化因子家族成员一起，CCL-17 与 RANTES、MIP-1a、MIP-1b、MCP-1、MCP-2、MCP-3 和 I-309 具有大约 24-29% 的氨基酸序列同一性。 TARC 在胸腺中表达，在肺、结肠和小肠中表达水平较低。此外，TARC 在受刺激的外周血单核细胞中瞬时表达。重组 TARC 已被证明对 T 细胞系具有趋化性，但对单核细胞或中性粒细胞没有。 CCL-17 是 CCR4 的特异性功能配体，CCR4 是一种在 T 细胞上选择性表达的受体。 CCL17 是聚集在 16 号染色体 q 臂上的少数 Cys-Cys 细胞因子基因之一。CCL17 对 T 淋巴细胞显示趋化活性，但对单核细胞或粒细胞不显示。 CCL17 与趋化因子受体 CCR4 和 CCR8 结合。这种趋化因子在胸腺 T 细胞发育以及成熟 T 细胞的运输和激活中起重要作用。

Description

Thymus and Activation Regulated Chemokine Rhesus Macaque Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 71 amino acid and having a molecular mass of approximately 8.1kDa.
TRAC is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的胸腺和活化调节的趋化因子恒河猴重组体是一种非糖基化多肽链，含有 71 个氨基酸，分子量约为 8.1kDa。
TRAC 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, 0.02 % Tween-20 and 300mM NaCl, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized Thymus and Activation Regulated Chemokine Rhesus Macaque in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TRAC although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Thymus and Activation Regulated Chemokine Rhesus Macaque should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

ARGTNVGREC CLKYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQNKAIC SDPNDKKVKK ALKYLQSLER S.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TARC Human
  • TARC Human, His
  • TARC Mouse
  • TARC Rat

ProSpec,TARC Mouse,胸腺和活化调节的趋化因子小鼠重组CHM-270

ProSpec,TARC Mouse,胸腺和活化调节的趋化因子小鼠重组CHM-270,TARC cDNA 编码一个 94 个氨基酸的前体蛋白和一个 23 个氨基酸残基的信号肽，该信号肽被切割掉以产生 71 个氨基酸残基的成熟分泌蛋白。

  • TARC Mouse

  • Thymus and Activation Regulated Chemokine Mouse Recombinant
  • CHM-270

Catalogue number

CHM-270

Synonyms

C-C motif chemokine 17, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, CC chemokine TARC, ABCD-2, CCL17, CCL-17, SCYA17, TARC, A-152E5.3, MGC138271, MGC138273.

Introduction

TARC cDNA encodes a 94 amino acid precursor protein with a 23 amino acid residue signal peptide that is cleaved off to generate the 71 amino acid residue mature secreted protein. Along with CC chemokine family members, CCL-17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1a, MIP-1b, MCP-1, MCP-2, MCP-3 and I-309. TARC is expressed in thymus, and at a lower level in the lung, colon, and small intestine. TARC is in addition transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant TARC has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL-17 was recently identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells. CCL17 is one of quite a few Cys-Cys cytokine genes clustered on the q arm of chromosome 16. CCL17 shows chemotactic activity for T lymphocytes, but not monocytes or granulocytes. CCL17 binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells.
TARC cDNA 编码一个 94 个氨基酸的前体蛋白和一个 23 个氨基酸残基的信号肽，该信号肽被切割掉以产生 71 个氨基酸残基的成熟分泌蛋白。与 CC 趋化因子家族成员一起，CCL-17 与 RANTES、MIP-1a、MIP-1b、MCP-1、MCP-2、MCP-3 和 I-309 具有大约 24-29% 的氨基酸序列同一性。 TARC 在胸腺中表达，在肺、结肠和小肠中表达水平较低。此外，TARC 在受刺激的外周血单核细胞中瞬时表达。重组 TARC 已被证明对 T 细胞系具有趋化性，但对单核细胞或中性粒细胞没有。 CCL-17 最近被确定为 CCR4 的特异性功能配体，CCR4 是一种选择性表达于 T 细胞上的受体。 CCL17 是聚集在 16 号染色体 q 臂上的大量 Cys-Cys 细胞因子基因之一。CCL17 对 T 淋巴细胞显示趋化活性，但对单核细胞或粒细胞没有趋化活性。 CCL17 与趋化因子受体 CCR4 和 CCR8 结合。这种趋化因子在胸腺 T 细胞发育以及成熟 T 细胞的运输和激活中起重要作用。

Description

TARC Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 70 amino acids and having a molecular mass of 7.9kDa.
The TARC Mouse is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH7.4.

Solubility

It is recommended to reconstitute the lyophilized TARC in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中重新配制冻干 TARC，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TARC although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TARC should be stored at 4C between 2-7 days and for future use below -18C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TARC 虽然在室温下可稳定保存 3 周，但应在 -18C 以下干燥储存。 重新配制后，TARC 应在 4C 下储存 2-7 天，以备将来在 -18C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

ARATNVGREC CLDYFKGAIP IRKLVSWYKT SVECSRDAIV FLTVQGKLIC ADPKDKHVKK AIRLVKNPRP.

Biological Activity

The ED50 as determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR4 is typically 2-10 ng/ml.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TARC Rat
  • TARC Human
  • TARC Human, His
  • TARC Macaque

ProSpec,ARC Rat,胸腺和活化调节的趋化因子大鼠重组CHM-012

ProSpec,ARC Rat,胸腺和活化调节的趋化因子大鼠重组CHM-012,CCL17 是聚集在 16 号染色体 q 臂上的大量 Cys-Cys 细胞因子基因之一。CCL17 对 T 淋巴细胞显示趋化活性，但对单核细胞或粒细胞没有趋化活性。

  • TARC Rat

  • Thymus and Activation Regulated Chemokine Rat Recombinant
  • CHM-012

Catalogue number

CHM-012

Synonyms

Thymus and activation-regulated chemokine, CCL17, SCYA17, TARC.

Introduction

TARC cDNA encodes a 94 amino acid precursor protein with a 23 amino acid residue signal peptide that is cleaved off to generate the 71 amino acid residue mature secreted protein. Along with CC chemokine family members, CCL-17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1a, MIP-1b, MCP-1, MCP-2, MCP-3 and I-309. TARC is expressed in thymus, and at a lower level in the lung, colon, and small intestine. TARC is in addition transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant TARC has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL-17 was recently identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells. CCL17 is one of quite a few Cys-Cys cytokine genes clustered on the q arm of chromosome 16. CCL17 shows chemotactic activity for T lymphocytes, but not monocytes or granulocytes. CCL17 binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells.
TARC cDNA 编码一个 94 个氨基酸的前体蛋白和一个 23 个氨基酸残基的信号肽，该信号肽被切割掉以产生 71 个氨基酸残基的成熟分泌蛋白。与 CC 趋化因子家族成员一起，CCL-17 与 RANTES、MIP-1a、MIP-1b、MCP-1、MCP-2、MCP-3 和 I-309 具有大约 24-29% 的氨基酸序列同一性。 TARC 在胸腺中表达，在肺、结肠和小肠中表达水平较低。此外，TARC 在受刺激的外周血单核细胞中瞬时表达。重组 TARC 已被证明对 T 细胞系具有趋化性，但对单核细胞或中性粒细胞没有。 CCL-17 最近被确定为 CCR4 的特异性功能配体，CCR4 是一种选择性表达于 T 细胞上的受体。 CCL17 是聚集在 16 号染色体 q 臂上的大量 Cys-Cys 细胞因子基因之一。CCL17 对 T 淋巴细胞显示趋化活性，但对单核细胞或粒细胞没有趋化活性。 CCL17 与趋化因子受体 CCR4 和 CCR8 结合。这种趋化因子在胸腺 T 细胞发育以及成熟 T 细胞的运输和激活中起重要作用。

Description

TARC Rat Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 8.1kDa.
The TARC Rat is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH7.4.

Solubility

It is recommended to reconstitute the lyophilized TARC in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TARC although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TARC should be stored at 4C between 2-7 days and for future use below -18C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TARC 虽然在室温下可稳定保存 3 周，但应在 -18C 以下干燥储存。 重新配制后，TARC 应在 4C 下储存 2-7 天，以备将来在 -18C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

ARATNVGREC CLDYFKGAIP IRKLVTWFRT SVECPKDAIV FETVQGRLIC TDPKDKHVKK AIRHLKNQRL.

Biological Activity

Determined by its ability to chemoattract human T-Lymphocytes using a concentration range of 1.0-10.0 ng/ml.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TARC Human, His
  • TARC Mouse
  • TARC Human
  • TARC Macaque

ProSpec,TECK Human,胸腺表达趋化因子人重组体CHM-364

CCL25是一种新型CC趋化因子，与其他CC趋化因子关系较远。 小鼠 CCL25 cDNA 也已被克隆并显示编码 144 a.a. 蛋白质，显示 49% a.a. 与人 CCL25 的序列同一性。ProSpec,TECK Human,胸腺表达趋化因子人重组体CHM-364

  • TECK Human

  • Thymus Expressed Chemokine Human Recombinant
  • CHM-364

Catalogue number

CHM-364

Synonyms

C-C motif chemokine 25, Small-inducible cytokine A25, Thymus-expressed chemokine, Chemokine TECK, CCL25, SCYA25, TECK, Ckb15, MGC150327.

Introduction

CCL25 is a novel CC chemokine, which is distantly related to other CC chemokines. The mouse CCL25 cDNA has also been cloned and shown to encode a 144 a.a. protein, which exhibits 49% a.a. sequence identity to the human CCL25. Human and mouse CCL25 expression was shown to be greatly restricted to the thymus and small intestine. While dendritic cells are identified as the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. CCL25 signals through the CCR9 receptor. Teck is possibly involved in T-cell development.
Recombinant human and mouse Teck were shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. The recombinant protein demonstrates chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritic cells but is inactive on peripheral blood lymphocytes and neutrophils.
CCL25是一种新型CC趋化因子，与其他CC趋化因子关系较远。 小鼠 CCL25 cDNA 也已被克隆并显示编码 144 a.a. 蛋白质，显示 49% a.a. 与人 CCL25 的序列同一性。 人和小鼠的 CCL25 表达被证明极大地局限于胸腺和小肠。 虽然树突细胞被确定为胸腺中 CCL25 产生的来源，但源自骨髓的树突细胞不表达 CCL25。 CCL25 通过 CCR9 受体发出信号。 Teck 可能参与了 T 细胞的发育。
重组人和小鼠 Teck 被证明对活化的巨噬细胞、树突细胞和胸腺细胞具有趋化性。 重组蛋白对胸腺细胞、巨噬细胞、THP-1 细胞和树突状细胞具有趋化活性，但对外周血淋巴细胞和中性粒细胞无活性。

Description

TECK Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14.2kDa.
The TECK is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from a concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized TECK in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TECK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TECK should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TECK 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重新配制后，TECK 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSRKNVSLL ISANSGL.

Biological Activity

Determined by its ability to chemoattract human monocytes using a concentration range of 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TECK Human, His
  • TECK Mouse

ProSpec-TECK Human His,胸腺表达趋化因子人重组体CHM-032

CCL25是一种新型CC趋化因子，与其他CC趋化因子关系较远。 小鼠 CCL25 cDNA 也已被克隆并显示编码 144 a.a. 蛋白质，显示 49% a.a. 与人 CCL25 的序列同一性。

  • TECK Human, His

  • Thymus Expressed Chemokine Human Recombinant, His Tag
  • CHM-032

Catalogue number

CHM-032

Synonyms

C-C motif chemokine 25, Small-inducible cytokine A25, Thymus-expressed chemokine, Chemokine TECK, CCL25, SCYA25, TECK, Ckb15, MGC150327.

Introduction

CCL25 is a novel CC chemokine, which is distantly related to other CC chemokines. The mouse CCL25 cDNA has also been cloned and shown to encode a 144 a.a. protein, which exhibits 49% a.a. sequence identity to the human CCL25. Human and mouse CCL25 expression was shown to be greatly restricted to the thymus and small intestine. While dendritic cells are identified as the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. CCL25 signals through the CCR9 receptor. Teck is possibly involved in T-cell development.
Recombinant human and mouse Teck were shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. The recombinant protein demonstrates chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritic cells but is inactive on peripheral blood lymphocytes and neutrophils.
CCL25是一种新型CC趋化因子，与其他CC趋化因子关系较远。 小鼠 CCL25 cDNA 也已被克隆并显示编码 144 a.a. 蛋白质，显示 49% a.a. 与人 CCL25 的序列同一性。 人和小鼠的 CCL25 表达被证明极大地局限于胸腺和小肠。 虽然树突细胞被确定为胸腺中 CCL25 产生的来源，但源自骨髓的树突细胞不表达 CCL25。 CCL25 通过 CCR9 受体发出信号。 Teck 可能参与了 T 细胞的发育。
重组人和小鼠 Teck 被证明对活化的巨噬细胞、树突细胞和胸腺细胞具有趋化性。 重组蛋白对胸腺细胞、巨噬细胞、THP-1 细胞和树突状细胞具有趋化活性，但对外周血淋巴细胞和中性粒细胞无活性。

Description

TECK Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 16.8kDa.
TECK is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Filtered colorless solution.

Formulation

TECK protein solution containing 20mM Tris-HCl buffer and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMQGVFE DCCLAYHYPI GWAVLRRAWT YRIQEVSGSC NLPAAIFYLP KRHRKVCGNP KSREVQRAMK LLDARNKVFA KLHHNTQTFQ AGPHAVKKLS SGNSKLSSSK FSNPISSSKR NVSLLISANS GL.

Purity

Greater than 90.0% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TECK Human
  • TECK Mouse

ProSpec,TECK Mouse,胸腺表达趋化因子小鼠重组CHM-259

ProSpec,TECK Mouse,胸腺表达趋化因子小鼠重组CHM-259,CCL25 通过 CCR9 受体发出信号。 Teck 可能参与了 T 细胞的发育。重组人和小鼠 Teck 被证明对活化的巨噬细胞、树突细胞和胸腺细胞具有趋化性。

  • TECK Mouse

  • Thymus Expressed Chemokine Mouse Recombinant
  • CHM-259

Catalogue number

CHM-259

Synonyms

C-C motif chemokine 25, Small-inducible cytokine A25, Thymus-expressed chemokine, Chemokine TECK, CCL25, SCYA25, TECK, Ckb15, MGC150327.

Introduction

CCL25 is a novel CC chemokine, which is distantly related to other CC chemokines. The mouse CCL25 cDNA has also been cloned and shown to encode a 144 a.a. protein, which exhibits 49% a.a. sequence identity to the human CCL25. Human and mouse CCL25 expression was shown to be greatly restricted to the thymus and small intestine. While dendritic cells are identified as the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. CCL25 signals through the CCR9 receptor. Teck is possibly involved in T-cell development.
Recombinant human and mouse Teck were shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. The recombinant protein demonstrates chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritic cells but is inactive on peripheral blood lymphocytes and neutrophils.
CCL25是一种新型CC趋化因子，与其他CC趋化因子关系较远。 小鼠 CCL25 cDNA 也已被克隆并显示编码 144 a.a. 蛋白质，显示 49% a.a. 与人 CCL25 的序列同一性。 人和小鼠的 CCL25 表达被证明极大地局限于胸腺和小肠。 虽然树突细胞被确定为胸腺中 CCL25 产生的来源，但源自骨髓的树突细胞不表达 CCL25。 CCL25 通过 CCR9 受体发出信号。 Teck 可能参与了 T 细胞的发育。
重组人和小鼠 Teck 被证明对活化的巨噬细胞、树突细胞和胸腺细胞具有趋化性。 重组蛋白对胸腺细胞、巨噬细胞、THP-1 细胞和树突状细胞具有趋化活性，但对外周血淋巴细胞和中性粒细胞无活性。

Description

TECK Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 14.1kDa. The TECK is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TECK 小鼠重组体是一条单一的、非糖基化的多肽链，含有 121 个氨基酸，分子量为 14.1kDa。 TECK 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from a concentrated solution in in 1×PBS, pH7.4.

Solubility

It is recommended to reconstitute the lyophilized TECK in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TECK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TECK should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Purity

Greater than 97.0% as determined by
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

QGAFEDCCLG YQHRIKWNVL RHARNYHQQE VSGSCNLRAV RFYFRQKVVC GNPEDMNVKR AIRILTARKR LVHWKSA QTERKKSNHM KSKVENPNST SVRSATLGHP RMVMMPRKTN N

Biological Activity

Determined by its ability to chemoattract human CCR9 transfected BaF3 mouse pro-B cells using a concentration range of 0.1-0.5 ug/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TECK Human, His
  • TECK Human

ProSpec,CCL16 Human,LEC/NCC-4 人重组CHM-237

ProSpec,CCL16 Human,LEC/NCC-4 人重组CHM-237,CCL16 对淋巴细胞和单核细胞显示趋化活性，而不是对中性粒细胞。 NCC-4 具有有效的骨髓抑制活性，抑制骨髓祖细胞的增殖。

  • CCL16 Human

  • LEC/NCC-4 Human Recombinant
  • CHM-237

Catalogue number

CHM-237

Synonyms

C-C motif chemokine 16, Small-inducible cytokine A16, IL-10-inducible chemokine, Chemokine LEC, Monotactin-1, Chemokine CC-4, Lymphocyte and monocyte chemoattractant, CCL-16, HCC-4, HCC4, NCC4, NCC-4, Liver Expressed Chemokine, LMC, LCC-1, LCC1, MTN-1, MTN1, SCYL4, ckB12, SCYA16, LEC, ILINCK, MGC117051.

Introduction

Human CCL16, also called HCC-4, liver-expressed chemokine , and lymphocyte and monocyte chemoattractant , is a novel CC chemokine recognized by bioinformatics. NCC-4 cDNA encodes a 120 amino acids along with a 23 amino acids signal peptide that is cleaved to generate 97 amino acid protein. HCC4 is vaguely related to other CC chemokines, showing less than 30% sequence identity. Among CC chemokines, CCL-16 has the largest similarity to HCC-1. 2 potential polyadenylation signals are present on the human HCC-4 gene, and as a result, 2 transcripts containing roughly 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, T cells, and some T cell clones. The expression of HCC-4 in monocytes is greatly upregulated in the presence of IL-10.
CCL16 shows chemotactic activity for lymphocytes and monocytes rather than to neutrophils. NCC-4 has potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. CCL16 demonstrates chemotactic activity for monocytes and thp-1 monocytes, rather than for resting lymphocytes and neutrophils. HCC-4 induces a calcium flux in thp-1 cells that desensitized prior to the expression of rantes.
人CCL16，又称HCC-4、肝表达趋化因子、淋巴细胞和单核细胞趋化因子，是生物信息学识别的新型CC趋化因子。 NCC-4 cDNA 编码一个 120 个氨基酸以及一个 23 个氨基酸的信号肽，该信号肽被切割产生 97 个氨基酸的蛋白质。 HCC4 与其他 CC 趋化因子模糊相关，显示小于 30% 的序列同一性。在 CC 趋化因子中，CCL-16 与 HCC-1 的相似性最大。人类 HCC-4 基因上存在 2 个潜在的多聚腺苷酸化信号，因此，已检测到包含大约 1,500 个碱基对和 500 个碱基对的 2 个转录本。 HCC-4 被一些淋巴细胞弱表达，包括 NK 细胞、T 细胞和一些 T 细胞克隆。在 IL-10 的存在下，单核细胞中 HCC-4 的表达大大上调。
CCL16 对淋巴细胞和单核细胞显示趋化活性，而不是对中性粒细胞。 NCC-4 具有有效的骨髓抑制活性，抑制骨髓祖细胞的增殖。 CCL16 对单核细胞和 thp-1 单核细胞表现出趋化活性，而不是对静息淋巴细胞和中性粒细胞。 HCC-4 在 thp-1 细胞中诱导钙通量，该细胞在 rantes 表达之前脱敏。

Description

CCL16 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 97 amino acids and having a molecular mass of 11.2 kDa.
The CCL16 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The CCL16 protein was lyophilized from a concentrated sterile solution containing 20mM PBS pH-7.4 and 0.15M sodium chloride.

Solubility

It is recommended to reconstitute the lyophilized CCL16in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized CCL16 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL16 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 CCL16 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CCL16 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

QPKVPEWVNTPSTCCLKYYEKVLPRRLVVGYRKALNCHLPAIIFVTKRNREVCTNP NDDWVQEYIKDPNLPLLPTRNLSTVKIITAKNGQPQLLNSQ.

Biological Activity

Determined by its ability to chemoattract total human monocytes using a concentration range of 10-100 ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec,FAM19A5 Human,具有序列相似性的家族 19 成员-A5 人类重组CHM-284

ProSpec,FAM19A5 Human,具有序列相似性的家族 19 成员-A5 人类重组CHM-284,在大肠杆菌中产生的 FAM19A5 人重组体是一种单一的、非糖基化的多肽链，包含 110 个氨基酸，包括 N 端的 10 个氨基酸的 His 标签。

  • FAM19A5 Human

  • Family With Sequence Similarity 19 Member-A5 Human Recombinant
  • CHM-284

Catalogue number

CHM-284

Synonyms

Family With Sequence Similarity 19 -Like), Member A5, Chemokine-Like Protein TAFA-5, TAFA5, TAFA Protein 5, QLLK5208, UNQ5208, TAFA-5, FAM19A5.

Introduction

Family With Sequence Similarity 19 Member-A5 belongs to the TAFA family which is comprised of 5 highly homologous genes encoding small secreted proteins. These proteins have conserved cysteine residues at fixed positions, and are remotely related to MIP-1alpha, which is a member of the CC-chemokine family. The TAFA proteins are primarily expressed in specific regions of the brain, and are assumed to serve as brain-specific chemokines or neurokines which act as regulators of immune and nervous cells.
具有序列相似性的家族 19 Member-A5 属于 TAFA 家族，该家族由 5 个高度同源的编码小分泌蛋白的基因组成。 这些蛋白质在固定位置具有保守的半胱氨酸残基，并且与作为 CC 趋化因子家族成员的 MIP-1alpha 有远程关系。 TAFA 蛋白主要在大脑的特定区域表达，并被假定为充当免疫和神经细胞调节剂的大脑特异性趋化因子或神经因子。

Description

FAM19A5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 110 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 12.2kDa.
在大肠杆菌中产生的 FAM19A5 人重组体是一种单一的、非糖基化的多肽链，包含 110 个氨基酸，包括 N 端的 10 个氨基酸的 His 标签。 总计算分子量为 12.2kDa。

Source

Escherichia Coli.

Physical Appearance

Filtered colorless solution.

Formulation

FAM19A5 filtered solution in 20mM Tris buffer, 50mM NaCl, pH 7.5 and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Amino acid sequence

MKHHHHHHASQFLKEGQLAA GTCEIVTLDR DSSQPRRTIA RQTARCACRK GQIAGTTRAR PACVDARIIK TKQWCDMLPC LEGEGCDLLI NRSGWTCTQP GGRIKTTTVS.

Purity

Greater than 95.0% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec,Lungkine Mouse,Lungkine 小鼠重组体CHM-286

小鼠 Lungkine/CXCL15 属于含有 ELR 基序的 CXC 趋化因子。小鼠 Lungkine 基因已被定位到 5 号染色体。ProSpec,Lungkine Mouse,Lungkine 小鼠重组体CHM-286

  • Lungkine Mouse

  • Lungkine Mouse Recombinant
  • CHM-286

Catalogue number

CHM-286

Synonyms

C-X-C motif chemokine 15, Lungkine, Small-inducible cytokine B15, Cxcl15, Scyb15.

Introduction

Mouse Lungkine/CXCL15 belongs to the ELR motif-containing CXC chemokines. The mouse Lungkine gene has been mapped to chromosome 5. The cDNA of mouse Lungkine encodes a 166 amino acids protein with a 25 aa predicted signal peptide and a 141 aa mature protein with an exceptionally long C-terminal tail which extends beyond beyond the chemokine fold. Lungkine protein is secreted into bronchoalveolar space and is involved in lung-specific neutrophils trafficking. Furthermore, studies in Lungkine knockout mice propose that Lungkine is an imperative mediator of neutrophil migration from the lung parenchyma into the airspace. In addition, Lungkine is chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation. By Northern blot analysis and in-situ hybridization, Lungkine transcripts have only been specifically detected in the adult and fetal lung, and its expression is up-regulated under inflammatory conditions. There is a 35% aa sequence similarity between the mouse Lungkine and the human ENA-78 and a 31% similarity with the human IL-8.
小鼠 Lungkine/CXCL15 属于含有 ELR 基序的 CXC 趋化因子。小鼠 Lungkine 基因已被定位到 5 号染色体。小鼠 Lungkine 的 cDNA 编码一个 166 个氨基酸的蛋白质，具有 25 个氨基酸的预测信号肽和一个 141 个氨基酸的成熟蛋白质，其 C 末端尾部非常长，延伸到趋化因子折叠之外. Lungkine 蛋白分泌到支气管肺泡空间并参与肺特异性嗜中性粒细胞运输。此外，对 Lungkine 基因敲除小鼠的研究表明，Lungkine 是中性粒细胞从肺实质迁移到空气空间的必要介质。此外，Lungkine 对骨髓祖细胞具有趋化作用并调节造血细胞分化。通过 Northern 印迹分析和原位杂交，仅在成人和胎儿肺中特异性检测到 Lungkine 转录物，并且其表达在炎症条件下上调。小鼠 Lungkine 与人类 ENA-78 之间的 aa 序列相似性为 35%，与人类 IL-8 的相似性为 31%。

Description

Recombinant Mouse Lungkine produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 142 amino acids and having a molecular mass of 16.4kDa.
The CXCL15 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The Lungkine protein was lyophilized from a 0.2µm filtered concentrated solution in PBS pH 7.4 and 0.02% Tween-20.

Solubility

It is recommended to reconstitute the lyophilized Lungkine in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中重新配制冻干的 Lungkine，然后可以将其进一步稀释成其他水溶液。

Stability

Lyophilized Lungkine although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL15 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 Lungkine 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CXCL15 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

QELRCLCIQE HSEFIPLKLI KNIMVIFETI YCNRKEVIAV PKNGSMICLD PDAPWVKATV GPITNRFLPE DLKQKEFPPA MKLLYSVEHE KPLYLSFGRP ENKRIFPFPI RETSRHFADL AHNSDRNFLR DSSEVSLTGS DA.

Biological Activity

The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 20-100 ng/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.