DiO, also called DiOC18(3), is a green fluorescent, lipophilic carbocyanine dye. DiO’s excitation max is 484 nm, and emission max is 501 nm.
DiO is widely used as an anterograde and retrograde neuronal tracer in living and fixed tissues and cells. DiO uniformly labels neurons via diffusion in the plasma membrane. In intact tissue, the dye does not transfer from labeled to unlabeled cells but some transfer may occur when the membrane is disrupted, for example after sectioning.
DiO and DiI are often used together in dual-color studies. But DiO has a slower lateral diffusion rate on the membranes than that of DiI.
>Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.
AF4568 is a bright, photostable, and hydrophilic fluorophore that emits in the orange channel. The absorption maximum is 572 nm. The emission maximum is 598 nm.
AF568 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells and whole organisms without the negative effect of copper ions on them, and inanimate samples.
Cyanine3 dibenzocyclooctyne (DBCO, or ADIBO) is a cycloalkyne dye derivative for copper free Click chemistry.
BDP 581/591 DBCO
BDP 581/591 is a borondipyrromethene dye that can be used not only as a fluorescent dye, but also as a ROS probe. It can be conjugated with azides using copper free Click chemistry.
Cyanine5.5 DBCO
Cyanine5.5 is a far-red / NIR emitting dye that is useful for bioimaging studies. Cyanine5.5 DBCO is a derivative of the dye with a cycloalkyne moiety for Copper free Click chemistry.
Cleavage of the N-glycosyl bond between a base and its 2-deoxyribose moiety in DNA generates an apurinic/apyrimidinic (so-called abasic) site. This phenomenon, which is referred to as depurination or depyrimidination occurs spontaneously under physiological conditions.
Phosphoramidites are modified nucleosides that are used in DNA and oligonucleotide synthesis. Abasic phosphoramidite replicates the loss of base pairing ability by a nucleotide. This modification can be useful when examining DNA damage and repair.
DiA is a dialkylaminostyryl dye that fluoresces when inserted into membranes or diluted into organic solvents. DiA’s excitation max is 460 nm, and emission max is 580 nm. Due to its broad emission spectrum, DiA can be detected in the green, orange, and red channels depending on the filter used.
DiA is widely used as an anterograde and retrograde neuronal tracer in living and fixed tissues and cells. DiA uniformly labels neurons via diffusion in the plasma membrane. In intact tissue, the dye does not transfer from labeled to unlabeled cells but some transfer may occur when the membrane is disrupted, for example after sectioning.
DiA can be used as a second-color neuronal tracer in conjunction with other neuronal tracers, for example, DiI.
Bicyclononyne (BCN) is a stable and one of the most reactive cyclooctynes for Copper-free click chemistry. Unlike dibenzocyclooctyne (DBCO), BCN is reactive both to azides (strain-promoted azyde-alkyne cycloaddition, SPAAC) and tetrazines (inverse electron demand Diels-Alder reaction, IEDDA). Being an endo-stereoisomer, Bicyclononine in endo-BCN CE-phosphoramidite provides a higher rate of cycloaddition compared to its exo-conformer.
BCN-labeled oligonucleotides may be used for the conjugation to azide- or tetrazine-containing solid surfaces, polymers, and large proteins.
Notes:
Coupling time is standard, like for amidites of natural nucleosides. Exclude the dimethoxytrityl (DMT) removal step and use the Dmt-ON protocol after amidite coupling and oxidation.
Use standard conditions for deprotection and ammonia solution, or AMA mixture (ammonium hydroxide / 40% methylamine, 1:1).
Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.
AF488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel. The absorption maximum is 495 nm. The emission maximum is 519 nm.
AF488 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.
Cyclooctyne derivative of fluorescein (FAM) for copper free Click chemistry reaction with azides. Contains pure 6-isomer of fluorescein.
3-Azidopropylamine
Bifunctional short crosslinker with an aminogroup and azide group.
BDP FL DBCO
BDP FL DBCO is a dye cyclooctyne for copper free Click chemistry reaction with azides. Absorption and emission wavelengths of BDP FL correspond to those of fluorescein (FAM), but BDP FL is significantly more photostable.
Alkyne derivative of AF488 dye for copper-catalyzed cycloaddition to azides (CuAAC). AF488 alkyne is useful for fluorescent labeling of azidated biomolecules, polymers, and surfaces. AF488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel (absorption max. is 495 nm, emission max. is 519 nm).
Lumizol reagent includes phenol, guanidine isothiocyanate and other components which makes the high-quality RNA extraction possible. Such components as phenol and guanidine isothiocyanate provide cell lysis and inhibit ribonucleases effectively. Thus the RNA is preserved intact.
After samples homogenization followed by the addition of chloroform the phase separation occurs: an upper, aqueous phase, the middle interphase and lower organic phase. RNA in the upper aqueous phase is then collected by precipitation with isopropanol, while DNA and proteins might be collected from the interphase and organic phase. DNA isolation from interphase and lower organic phase can be performed using ethanol solution and for proteins by precipitation with isopropanol from supernatant.
Indocyanine green (aka ICG, IC Green, Foxgreen) is used for reliable imaging and flow cytometry. This dye is water soluble and pH-insensitive. Fluorescence of ICG is not visible to the human eye but is readily detected by most imaging systems.
General properties
Appearance:
dark green powder
Molecular weight:
774,96
CAS number:
3599-32-4
Molecular formula:
C43H47N2NaO6S2
Quality control:
NMR 1H and 31P, HPLC-MS (95%)
Storage conditions:
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Texas Red (TR) is a sulfo-analog of ROX dye. It has similar spectral properties, and emits in red region of the spectrum. TR X is a dye with aminohexanoic acid linker, that provides spacing between the fluorophore and the biomolecule, to prevent undesired interactions.
NHS ester is reactive towards amine groups that are ubiquitous in proteins, peptides and other labeling targets.
The Universal CPG type II, 1000A is one of universal supports used to immobilize nucleosides for synthesizing oligonucleotides and to increase rate of dephosphorylation of the 3′ end oligonucleotide during deblocking.
For the cleavage from the support and oligonucleotide deprotection anhydrous ammonia gas-phase, ammonium hydroxide/methylamine mixture and other basic reagents can be used in a short time. The Universal CPG type II, 1000A is suitable for use in harsh conditions and makes cleavage and deprotection faster compared to universal supports. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides up to 120 bases.
Usage
Coupling: Standard conditions for universal CPG.
Deprotection: 2 hours at 80 °C or 8 hours at 55 °C using concentrated ammonia; 15 minutes at 65 °C using AMA mixture, ammonium hydroxide – 40% methylamine (1:1).
Controlled pore glass (CPG) 1000 modified support for the synthesis of oligonucleotides with DusQ2 quencher at the 3’ end. Compatible with standard deblocking conditions.
DusQ1 CPG 1000
Controlled pore glass (CPG) 1000 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.
Amine-PEG3-carboxylic acid is a bifunctional PEG3-derivative containing terminal carboxyl and amino groups. Hydrophilic PEG3 spacer is freely soluble in water.
The terminal amino group can react with activated esters, carbonyl compounds, and carbonic acids, e.g. in reductive amination reactions.
The carbonyl group in amine-PEG3-carboxylic acid can react with primary amino groups in the presence of activators (carbodiimides or HATU) resulting in a stable amide bond.
VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.
This phosphoramidite reagent allows to introduce the label onto 5′-terminus of the oligonucleotide.
DusQ21, identical to QSY21, is a quencher for the dyes in far red region of the spectrum. The dye is a xanthene derivative, unlike DusQ1 or DusQ2, that are azo dyes. It has a high extinction coefficient, and provides an outstanding quenching efficiency.
The controlled pore glass solid support allows to synthesize oligonucleotides carrying the dye moiety on 3′-terminus.
General properties
Appearance:
dark blue beads
Quality control:
1H NMR of bound reagent, coupling testing with HPLC-MS
Storage conditions:
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
DusQ3 is a dark quencher for far red and near infrared fluorophores, especially suitable for Cyanine5.5.
The dye can be attached to 3′-terminus of oligonucleotides by means of automated solid phase synthesis using this support. The dye is sensitive to nucleophilic reagents, and therefore must be carefully deprotected. We recommend the use of ultramild, phenoxyacetyl protected monomers. As an alternative, post-synthetic labeling using NHS ester of the quencher can be used.
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DusQ1 CPG 500
Controlled pore glass (CPG) 500 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.
DusQ2 CPG 500
Controlled pore glass (CPG) 500 modified support for the synthesis of oligonucleotides with DusQ2 quencher at the 3’ end. Compatible with standard deblocking conditions.
Appearance:
dark blue beads
Quality control:
1H NMR of bound reagent, coupling testing with HPLC-MS
Storage conditions:
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS:
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Product specifications
Oligo synthesis details
Pore size, Å:
500
Typical loading, umol/g:
50-80
Coupling conditions:
standard, ultramild phosphoramidites
Cleavage conditions:
2 h / rt, 30% aq. ammonia (ultramild protective groups)
The Universal CPG type I, 500A is one of universal supports used to immobilize nucleosides for high-throughput synthesizing oligonucleotides.
The cleavage from the support is followed by deprotection using anhydrous methylamine gas, ammonium hydroxide/methylamine mixture and other basic reagents. Universal support CPG type I is suitable for relatively aggressive environment treatment. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases.
Usage
Coupling: Standard conditions for universal CPG.
Deprotection: 8 hours at 80 °C using ammonium hydroxide or 17 hours at 55 °C; 3 hours at 80 °C using AMA mixture, ammonium hydroxide – 40% methylamine (1:1) or 8 hours at 55 °C using 40% methylamine or 30 minutes at 80 °C; 3 hours at 55 °C; 17 hours at room temperature using 0.4M NaOH in methanol – water (4:1).
Controlled pore glass (CPG) 500 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.
This fluorescent conjugate is useful to construct streptavidin-based fluorescent assays and visualize binding of biomolecules. This is especially useful because streptavidin has four binding centers for biotin – this gives opportunity to build different kinds of assays. For example, target molecule having affinity to surface can be conjugated with biotin and be immobilized on solid surface. After it, streptavidin can be bound to the biotinylated surface and washed. Then, bound streptavidin on the surface can be visualized with biotin-dye conjugate.
The long and hydrophilic PEG3 linker facilitates binding and decreases nonspecific interactions.
Sulfo-Cyanine7.5 is an amine derivative of water soluble, hydrophilic sulfonated Cyanine7.5 dye.
The dye is useful as a label for in vivo NIR imaging. The primary amine group can be conjugated with various electrophilic reagents, like activated carboxylic acid derivatives (for example, NHS, PFP esters), epoxides, and others.
Azidopropylamine is a short bifunctional molecule with an azide and amine group. Primary amine group possesses high reactivity towards activated carboxylic derivatives like NHS esters.
Azide group can be coupled with alkynes in CuAAc reaction. Both reactions are orthogonal, and can be carried out independently.
The reagent is useful for the azidation of peptide C-terminus or side chains of aspartic acid and glutamic acid after their activation.
Azidopropanol is a bifunctional synthone carrying azide group and hydroxy group. It can be used for the Click chemistry or Staudinger ligation involving its azide group.
Hydroxy group can be functionalized in non-aqueous environment by means of reaction with CDI and subsequent coupling with amines producing carbamates.
TAMRA (tetramethylrhodamine) is a xanthene dye with a fluorescence maximum at 567 nm.
This product is the 6-TAMRA N-Hydroxysuccinimide (NHS)-ester. TAMRA NHS-ester readily reacts with various amines and is used to generate fluorescently labeled proteins, peptides, antibodies and other biomolecules.
Storage: 12 months after receipt at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Rhodamine 6G (R6G) is a xanthene dye of rhodamine series that has been used for the labeling of oligonucleotides and DNA for quite a long time. Just like other xanthenes, R6G comes as two isomers, 5- and 6-isomer. Even though their absorption and emission spectra are virtually indistinguishable, the isomers need to be separated to avoid separation of the labeled molecules during their purification, i.e. double HPLC peaks and double spots on electrophoresis.
This is an amine reactive NHS ester derived from a pure 5-isomer of R6G. Rhodamine 6G is very bright (even used as a quantum yield standard, yield 0.95). The dye is soluble in organic solvents, such as DMF, or DMSO. They can be used as co-solvents for the labeling reactions.
Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides.
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ROX NHS ester, 5-isomer
Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
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ROX NHS ester, 6- isomer
Pure isomer 6-ROX NHS ester for amine labeling.
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General properties
Appearance:
dark colored solid
Molecular weight:
555.58
Molecular formula:
C31H29N3O7
Solubility:
good in DMF, DMSO
Quality control:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
lumiprobe -Cyanine3B carboxylic acid,2321-1mg,Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Products
Reactive dyes
Carboxylic acids
Cyanine3B carboxylic acid
Cyanine3B carboxylic acid
Cyanine3, Carboxylic acids, sulfo-Cyanine3,Cy3
Cat. #
Quantity
Price
Lead time
2321-1mg
1 mg
110.00$
8 days
2321-5mg
5 mg
149.00$
8 days
2321-25mg
25 mg
690.00$
8 days
2321-50mg
50 mg
1290.00$
8 days
2321-100mg
100 mg
2450.00$
8 days
General properties
Appearance:
dark red powder
Molecular weight:
560.66
Molecular formula:
C31H32N2O6S
Solubility:
good in DMF, DMSO
Quality control:
NMR 1H, HPLC-MS (95%)
Storage conditions:
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
lumiprobe,AF647 carboxylic acid,Reactive dyes,Carboxylic acids, good in DMSO, DMF, water,Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.