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Products > Crystallization Plates, Hardware & Accessories > Dialysis Buttons, Membranes & Applicators > Dialysis Buttons
Dialysis Buttons
应用
- Crystallization by dialysis, protein folding, & small volume sample dialysis
- 通过透析、蛋白质折叠和小体积样品透析进行结晶
特征
- Low volume dialysis
- Fits in 24 well plates
- Use over and over again
- 低容量透析
适合 24 孔板
反复使用
描述
In the dialysis method, the sample in question is separated from the “precipitant” by a semi-permeable membrane which allows small reagent molecules to pass but prevents biological macromolecules from crossing the membrane.1-3
Dialysis Buttons are injection molded from polystyrene, and are the size of a small button. The sample is placed in this chamber so as to create a slight dome of liquid at the top of the button. A dialysis membrane is placed over the top of the button/sample and is held in place with an O-ring. The O-ring is held in place by a groove in the Dialysis Button. Dialysis Buttons are supplied with O-rings. The 5 µl to 100 µl sized dialysis buttons have an approximate overall height of 6 mm, an approximate outside diameter of 10 mm without an O-ring in place, and an approximate diameter of 12 mm with an O-ring in place. The 200 µl and 350 µl sized dialysis buttons have an approximate overall height of 8 mm, an approximate outside diameter of 15 mm without an O-ring in place, and an approximate diameter of 17 mm with an O-ring in place. Each package of dialysis buttons includes a plastic golf tee shaped applicator to assist with applying a dialysis membrane to the button and securing with the supplied o-ring.
在透析方法中，有问题的样品通过半透膜与“沉淀剂”分离，半透膜允许小试剂分子通过，但阻止生物大分子穿过膜。1-3
透析按钮由聚苯乙烯注塑成型，大小与小按钮相当。将样品放置在该腔室中，以便在按钮顶部形成一个轻微的液体圆顶。透析膜放置在按钮/样品的顶部，并用 O 形环固定到位。 O 形环由透析按钮中的凹槽固定到位。透析按钮配有 O 形圈。 5 µl 至 100 µl 大小的透析按钮的总高度约为 6 mm，外径约为 10 mm，未安装 O 形圈，直径约为 12 mm，安装了 O 形圈。 200 µl 和 350 µl 大小的透析按钮总高度约为 8 mm，外径约为 15 mm，不带 O 形圈，直径约为 17 mm，带 O 形圈。每个透析按钮包都包括一个塑料高尔夫球 T 形涂抹器，以帮助将透析膜应用到按钮上并使用随附的 O 形圈固定。
HR3-336 Dialysis Buttons Sampler
CAT NO
HR3-336
NAME
DESCRIPTION
5 of each size listed below
PRICE
$105.00
CAT NO
HR3-314
NAME
DESCRIPTION
50 pack
PRICE
$105.00
CAT NO
HR3-316
NAME
DESCRIPTION
50 pack
PRICE
$105.00
CAT NO
HR3-318
NAME
DESCRIPTION
50 pack
PRICE
$105.00
CAT NO
HR3-320
NAME
DESCRIPTION
50 pack
PRICE
$105.00
CAT NO
HR3-326
NAME
DESCRIPTION
50 pack
PRICE
$105.00
CAT NO
HR3-328
NAME
DESCRIPTION
50 pack
PRICE
$105.00
CAT NO
HR3-330
NAME
DESCRIPTION
50 pack
PRICE
$105.00
CAT NO
HR3-332
NAME
DESCRIPTION
50 pack
PRICE
$105.00
支持材料
Dialysis Button User Guide相关项目
- Dialysis Membrane Discs for Buttons
- 用于按钮的透析膜盘
参考
1. McPherson, A., Preparation and Analysis of Protein Crystals, Krieger Publishing, 88-91 (1992).
2. Durcruix, A, and Giege, R., Crystallization of Nucleic Acids and Proteins, A Practical Approach, Oxford University Press, 78-82 (1992).
3. Zeppenzauer, M., et al., Acta Chem Scan (1967) 21, 1009.
4. Structure of the membrane domain of respiratory complex I. Efremov RG, Sazanov LA. Nature. 2011 Aug 7;476(7361):414-20. doi: 10.1038/nature10330.
1. McPherson, A.，蛋白质晶体的制备和分析，Krieger 出版社，88-91 (1992)。
2. Durcruix, A 和 Giege, R.，核酸和蛋白质的结晶，实用方法，牛津大学出版社，78-82 (1992)。
3. Zeppenzauer, M. 等人，Acta Chem Scan (1967) 21, 1009。
4. 呼吸复合体膜结构域的结构 I. Efremov RG, Sazanov LA。 自然。 2011 年 8 月 7 日；476(7361):414-20。 doi：10.1038/nature10330。
HR3-310 HR3-312 Micro-Bridges User Guide
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