Hampton蛋白结晶试剂盒Seeding Tool

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Products > Tools & Seeding > Seeding Tool > Seeding Tool

Seeding Tool

应用

Streak Seeding

特征

Natural fiber 天然纤维
Built-in handle/cover 内置把手/盖子

描述

During streak seeding, one touches the Seeding Tool to crystalline material to dislodge, remove and transfer small crystals (seeds) to a drop that will support the growth of potentially larger and more perfect crystals.

A seed can provide a template on which additional macromolecules can assemble and under the proper conditions, grow to form a large single crystal. Using seeding can avoid problems associated with growing crystals from spontaneous nucleation. Seeds can grow into larger crystals in the metastable region of the solubility curve, which is a region of lower, relative supersaturation. One can also streak seed from phase separation or amorphous material as a diagnostic to confirm whether the material is crystalline.

The Seeding Tool is a 1 cm natural fiber attached to a stainless steel pin and plastic handle. The Seeding Tool is supplied with a cover to protect the fiber and when the cover is placed on the back side of the Seeding Tool, it makes an excellent handle

在条纹播种过程中，将播种工具接触到结晶材料以去除、移除小晶体（种子）并将其转移到一滴中，这将支持可能更大、更完美的晶体的生长。

种子可以提供模板，额外的大分子可以在其上组装，并在适当的条件下生长形成大的单晶。使用晶种可以避免与自发成核生长晶体相关的问题。晶种可以在溶解度曲线的亚稳态区域中生长成较大的晶体，该区域是较低的相对过饱和区域。人们还可以从相分离或无定形材料中划出种子作为诊断，以确认材料是否为结晶。

播种工具是一根 1 厘米长的天然纤维，附在不锈钢针和塑料手柄上。播种工具配有保护纤维的盖子，当盖子放在播种工具的背面时，它是一个出色的手柄

CAT NO

HR8-133

NAME

Seeding Tool

DESCRIPTION

5 pack

PRICE

$39.00

参考

1. Seeds to crystals. Terese Bergfors. Journal of Structrual Biology 142 (2003) 66-76.

2. Applications of the streak seeding technique in protein crystallization. Stura and Wilson. Journal of Crystal Growth, 110 (1991) 270-282. 110 (1991) 270-282.

3. Applications of the streak seeding technique in protein crystallization. Stura and Wilson. Journal of CrystaL Growth, 110 (1991) 270-282.

4. Crystallization of pig mitochondrial aspartate aminotransferase by seeding with crystals of the chicken mitochondrial isoenzyme. Eichele G, Ford GC, Jansonius JN. J Mol Biol. 1979 Dec 5;135(2):513-6. PubMed PMID: 537086.

1. 种子到晶体。特蕾西·伯格福斯。结构生物学杂志 142 (2003) 66-76。

2.划线接种技术在蛋白质结晶中的应用。斯图拉和威尔逊。晶体生长杂志，110 (1991) 270-282。 110 (1991) 270-282。

3. 划线接种技术在蛋白质结晶中的应用。斯图拉和威尔逊。晶体生长杂志，110 (1991) 270-282。

4.通过接种鸡线粒体同工酶晶体来结晶猪线粒体天冬氨酸氨基转移酶。 Eichele G，福特 GC，Jansonius JN。 J摩尔生物学。 1979 年 12 月 5 日；135(2):513-6。 PubMed PMID：537086。

Hampton蛋白结晶试剂盒Sodium acetate trihydrate Buffer

Products > Optimize Reagents > Optimize – Buffers > Sodium acetate trihydrate Buffer

Sodium acetate trihydrate Buffer

应用

Crystallization grade Sodium acetate trihydrate buffer for formulating screens or for optimization
结晶级三水乙酸钠缓冲液，用于配制筛选或优化

特征

Sterile filtered 无菌过滤
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

Formulated from Sodium acetate trihydrate

由醋酸钠三水合物配制而成

Synonyms: Acetic acid sodium salt trihydrate

别名：醋酸钠盐三水合物
C₂H₃NaO₂ • 3H₂O
CH₃COONa • 3H₂O
M_r 136.08
CAS Number [6131-90-4]
EC Number 204-823-8
Merck 14,8571
Beilstein Registry Number 3732037
RTECS AJ4580000
MDL Number MFCD00071557
PubChem Substance ID 24886015
Purity ≥ 99.5%
pKa = 4.8

The useful pH range of a Sodium acetate trihydrate buffer is pH 3.6 – 5.6.

Titrate HR2-569 to the desired pH using HCl
HR2-569 Measured pH Range: 8.7 – 9.3 at 25°C
HR2-569 Measured Conductivity Range: 48.0 – 51.7 mS/cm at 25°C
HR2-569 Measured Refractive Index Range: 1.34395 – 1.34415 at 20°C

HR2-789 has been titrated to pH 4.5 using HCl
HR2-789 Measured Conductivity Range: 60.8 – 67.7 mS/cm at 25°C
HR2-789 Measured Refractive Index Range: 1.34530 – 1.34579 at 20°C

HR2-731 has been titrated to pH 4.6 using HCl
HR2-731 Measured Conductivity Range: 59.3 – 64.5 mS/cm at 25°C
HR2-731 Measured Refractive Index Range: 1.34535 – 1.345745 at 20°C

Al ≤0.0005%
As ≤0.00001%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Ni ≤0.0005%
Pb ≤0.0005%
PO₄ ≤0.0005%
SO₄ ≤0.002%
Sr ≤0.0005%
Zn ≤0.0005%

Sodium acetate trihydrate

CAT NO

HR2-569

NAME

1.0 M Sodium acetate trihydrate

DESCRIPTION

100 mL

PRICE

$41.00

CAT NO

HR2-789

NAME

1.0 M Sodium acetate trihydrate pH 4.5

DESCRIPTION

100 mL

PRICE

$41.00

CAT NO

HR2-731

NAME

1.0 M Sodium acetate trihydrate pH 4.6

DESCRIPTION

100 mL

PRICE

$41.00

参考

1. Crystal structure of thioltransferase at 2.2 A resolution. Katti, SK; Robbins, AH; Yang, Y; Wells, WW. Protein Sci 4, 1998- 2005, 1995.

Hampton蛋白结晶试剂盒StockOptions Sodium Acetate Buffer Kit

Products > Optimization Screens > StockOptions Buffer Kits > StockOptions Sodium Acetate Buffer Kit

StockOptions Sodium Acetate Buffer Kit

应用

Focused, titrated buffer stocks for crystal screening, optimization, & production
用于晶体筛选、优化和生产的集中滴定缓冲液

特征

0.1 unit increments for fine tuning pH
pH 3.6 – 5.6
1.0 M concentrated stocks
pKa = 4.8

描述

StockOptions Buffer kits are reagent toolboxes for the macromolecular crystal grower. Stock-Options reagent kits offer precisely formulated, high quality crystallization grade reagent stocks in convenient, cost-effective kits. The chemicals utilized in StockOptions kits are the same crystallization grade, ultra-high purity chemicals utilized in the Hampton Research kits. StockOptions reagents are carefully formulated under strict quality standards to ensure reliable performance and lot-to-lot consistency.

Preformulated stocks remove calculation, measurement, and formulation errors. No more second guessing how the reagent was formulated, what specific chemical was used, when it was made, or how to precisely reproduce that reagent when it is gone and more reagent is required for additional setups.

Each HR2-233 contains 21 unique reagents covering the pH range of 3.6 to 5.6 in 0.1 pH increments. Each reagent is supplied in a 10 milliliter volume and is titrated using HCl.

StockOptions 缓冲液试剂盒是大分子晶体生长器的试剂工具箱。 Stock-Options 试剂盒以方便、经济高效的试剂盒形式提供精确配制的高质量结晶级试剂库存。 StockOptions 套件中使用的化学品与 Hampton Research 套件中使用的结晶级超高纯度化学品相同。 StockOptions 试剂按照严格的质量标准精心配制，以确保可靠的性能和批次间的一致性。

预先配制的库存消除了计算、测量和配方错误。无需再猜测试剂是如何配制的、使用了何种特定化学品、何时制造，或者在试剂消失后如何精确复制该试剂，并且需要更多试剂来进行额外设置。

每个 HR2-233 包含 21 种独特的试剂，涵盖 3.6 至 5.6 的 pH 范围，以 0.1 pH 为增量。每种试剂的容量为 10 毫升，并使用 HCl 进行滴定。

Sodium acetate trihydrate
Synonym: Acetic acid sodium salt trihydrate
C₂H₃NaO₂·3H₂O
CH₃COONa·3H₂O
M_r 136.08
CAS Number [6131-90-4]
EC Number 204-823-8
Merck 14,8571
Beilstein Registry Number 3732037
RTECS AJ4580000
MDL Number MFCD00071557
PubChem Substance ID 24886015
Purity >99.5%
pKa = 4.8

Assay
Al <0.0005%
As <0.00001%
Bi <0.0005%
Ca <0.001%
Cd <0.0005%
Cl <0.0005%
Co <0.0005%
Cr <0.0005%
Cu <0.0005%
Fe <0.0005%
K <0.005%
Li <0.0005%
Mg <0.0005%
Mn <0.0005%
Mo <0.0005%
Ni <0.0005%
Pb <0.0005%
PO₄ <0.0005%
SO₄ <0.002%
Sr <0.0005%
Zn <0.0005%

HR2-233 StockOptions Sodium Acetate Buffer Kit 10 mL tube format

CAT NO

HR2-233

NAME

StockOptions Sodium Acetate Kit

DESCRIPTION

pH 3.6 to 5.6 in 0.1 pH unit increments

PRICE

$265.00

参考

1. Microscale Fluorescent Thermal Stability Assay for Membrane Proteins Structure, Volume 16, Issue 3, 11 March 2008, Pages 351-359 Alexander I. Alexandrov, Mauro Mileni, Ellen Y.T. Chien, Michael A. Hanson, Raymond C. Stevens

2. Crystal structures of reduced, oxidized, and mutated human thioredoxins: evidence for a regulatory homodimer. Weichsel, A; Gasdaska, JR; Powis, G; Montfort, WR. Structure 4, 735- 751, 1996.

3. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.

1. 膜蛋白结构的微尺度荧光热稳定性分析，第 16 卷，第 3 期，2008 年 3 月 11 日，第 351-359 页 Alexander I. Alexandrov、Mauro Mileni、Ellen Y.T. Chien、迈克尔 A. 汉森、雷蒙德 C. 史蒂文斯

2. 还原、氧化和突变的人硫氧还蛋白的晶体结构：调节同源二聚体的证据。魏克塞尔，一个；加斯达斯卡，JR；鲍伊斯，G；蒙福特，WR。结构 4, 735-751, 1996。

3. 通过对 pH 限值进行精细采样来增加微晶的尺寸。 A.麦克弗森。 J.应用。水晶。（1995 年）。 28, 362-365。

Hampton蛋白结晶试剂盒Grid Screen Sodium Chloride

Products > Crystallization Screens > Grid Screens > Grid Screen Sodium Chloride

Grid Screen Sodium Chloride

应用

Primary or secondary, Sodium chloride versus pH grid crystallization screen for biological macromolecules
用于生物大分子的初级或次级氯化钠与 pH 网格结晶筛选

特征

A systematic grid screen varying Sodium chloride concentration versus pH
Samples pH 4 to 9
Samples four concentrations of Sodium chloride
Preciase pH versus Sodium chloride screen
- pH adjusted after salt addition; 25°Celsius
Combine reagents between rows or columns to create expanded grid screens
改变氯化钠浓度与 pH 值的系统网格筛选
样品 pH 值 4 至 9
采样四种浓度的氯化钠
精确 pH 值与氯化钠筛选
加盐后调节pH； 25°摄氏度
在行或列之间组合试剂以创建扩展的网格屏幕

描述

Grid Screen™ Sodium Chloride is a preformulated reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules.

The screen is simple and practical for finding initial crystallization conditions as well as determining the solubility of a macromolecule in Sodium chloride between pH 4.0 and 9.0.

Grid Screen Sodium Chloride evaluates Sodium chloride (HR2-219) at four concentrations (1.0, 2.0, 3.0, 4.0 M) versus six pH levels (4, 5, 6, 7, 8, 9).

Grid Screen Sodium Chloride consists of 24 unique reagents, each supplied as 10 milliliter in sterile, screw cap tubes. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salt and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Grid Screen™ 氯化钠是一种预先配制的试剂盒，旨在为生物大分子的结晶提供快速筛选方法。

该屏幕简单实用，可用于寻找初始结晶条件以及确定大分子在 pH 4.0 和 9.0 之间的氯化钠中的溶解度。

Grid Screen Sodium Chloride 评估四种浓度（1.0、2.0、3.0、4.0 M）的氯化钠 (HR2-219) 与六种 pH 水平（4、5、6、7、8、9）的对比。

Grid Screen 氯化钠由 24 种独特的试剂组成，每种试剂以 10 毫升无菌螺旋盖管形式提供。即用型试剂经过无菌过滤和超纯 1 型水配制，使用最高纯度的盐和缓冲液。个别试剂可通过 Hampton Research Custom Shop 购买。

CAT NO

HR2-219

NAME

Grid Screen Sodium Chloride

DESCRIPTION

10 ml, tube format

PRICE

$138.00

参考

1. Advance in Protein Chemistry Volume 41. Pages 1-33 (Patricia C. Weber). Academic Press, 1991.

2. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.

3. Current approaches to macromolecular crystallization. McPherson, A. Eur. J. Biochem. 189, 1-23, 1990.

4. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.

5. A protein crystallization strategy using automated grid searches on successively finer grids. Patricia C. Weber. Methods: A Companion to Methods in Enzymology Vol. 1, No. 1, August, pp. 31-37, 1990.

1. 蛋白质化学进展第 41 卷。第 1-33 页（Patricia C. Weber）。学术出版社，1991。

2. 核酸和蛋白质的结晶，A. Ducruix 和 R. Giege 编辑，实用方法系列，牛津大学。出版社，1992 年。

3. 大分子结晶的当前方法。麦克弗森，A. Eur。 J.生化。 189, 1-23, 1990。

4. 蛋白质和核酸结晶。方法，酶学方法指南，学术出版社，第 1 卷，第 1 期，1990 年 8 月。

5. 在连续更细的网格上使用自动网格搜索的蛋白质结晶策略。帕特里夏 C. 韦伯。方法：酶学方法的伴侣卷。 1，第 1 期，8 月，第 31-37 页，1990 年。

Hampton蛋白结晶试剂盒ADA Buffer

Products > Optimize Reagents > Optimize – Buffers > ADA Buffer

ADA Buffer

应用

Crystallization grade ADA buffer for formulating screens or for optimization
用于配制筛选或优化的结晶级 ADA 缓冲液

特征

Sterile filtered 无菌过滤
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

ADA (Buffer)

Synonyms: N-(2-Acetamido)iminodiacetic acid;
N-(Carbamoylmethyl)iminodiacetic acid
C₆H₁₀N₂O₅
H₂NCOCH₂N(CH₂CO₂H)₂
M_r 190.16
CAS Number [26239-55-4]
EC Number 247-530-0
Beilstein Registry Number 1787181
Merck 14,147
Purity ≥ 99.0%
MDL Number MFCD00008031
PubChem Substance ID 24845005
pKa = 6.6 at 25°Celsius

Useful pH range of ADA is 5.6 – 7.5

Titrate HR2-507 to the desired pH using NaOH
HR2-817 is titrated to pH 6.5 using NaOH

HR2-507 measured Conductivity range: 23.6 – 28.6 mS/cm at 25°C
HR2-507 measured Refractive Index range: 1.35009 – 1.35044 at 20°C

HR2-817 measured Conductivity range: 42.2 – 50.4 mS/cm at 25°C
HR2-817 measured Refractive Index range: 1.36787 – 1.36913 at 20°C

ADA buffer is used in the Hampton Research MembFac crystallization kit.ADA 缓冲液用于 Hampton Research MembFac 结晶试剂盒。

Total Impurities Insoluble matter, passes filter test总杂质不溶物，通过过滤测试
Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.1%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.05%
Ni ≤0.0005%
Pb ≤0.0005%
SO₄ ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%

CAT NO

HR2-507

NAME

0.5 M ADA

DESCRIPTION

100 mL

PRICE

$123.00

CAT NO

HR2-817

NAME

1.0 M ADA pH 6.5

DESCRIPTION

100 mL

PRICE

$132.00

SDS

参考

1. Structures of the noncovalent complexes of human and bovine prothrombin fragment 2 with human PPACK-thrombin. Arni, RK; Padmanabhan, K; Padmanabhan, KP; Wu, TP; Tulinsky, A. Biochemistry 32, 4727- 4737, 1993.

2. Structures of Three Crystals Forms of the Sweet Protein Thaumatin. Ko, TP; Day, J; Greenwood, A; McPherson, A. Acta Crystallogr D 50, 813- 825, 1994.

1.人和牛凝血酶原片段2与人PPACK-凝血酶的非共价复合物的结构。阿尼，RK；帕德马纳班，K；帕德马纳班，KP；吴，TP； Tulinsky, A. Biochemistry 32, 4727-4737, 1993。

2. 甜蛋白奇异果甜蛋白的三种晶型结构。高，TP；日，J；格林伍德，一个； McPherson, A. 晶体学学报 D 50, 813-825, 1994。

Hampton蛋白结晶试剂盒Acetic acid

Products > Optimize Reagents > Optimize – Buffers > Acetic acid

Acetic acid

应用

Crystallization grade Acetic acid for altering drop and reservoir pH
结晶级乙酸，用于改变液滴和储液器的 pH 值

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Acetic acid

Synonyms: Glacial acetic acid
CAS Number [64-19-7]
CH₃CO₂H
M_r 60.05
Beilstein Registry Number 506007
EC Number 200-580-7
MDL number MFCD00036152
PubChem Substance ID 24859247

See the pdf Using Volatile Buffers for a novel crystallization methodology using acetic acid and ammonium hydroxide to adjust drop pH and induce crystallization.

别名：冰醋酸
CAS 编号 [64-19-7]
CH3CO2H
60.05 先生
贝尔斯坦注册号 506007
欧盟编号 200-580-7
MDL 编号 MFCD00036152
PubChem 物质 ID 24859247

有关使用乙酸和氢氧化铵调节液滴 pH 值并诱导结晶的新型结晶方法，请参阅 pdf 使用挥发性缓冲液。

Acetic acid

CAT NO

HR2-853

NAME

5.2 M Acetic acid

DESCRIPTION

15 mL

PRICE

$24.00

参考

1. Preparation and analysis of protein crystals. Alexander McPherson, 1982, John Wiley and Sons, Inc., printed by Krieger Publishing. Chapter 4, Crystallization.

2. Current approaches to macromolecular crystallization. Alexander McPherson, Eur. J. Biochem. 189, 1-23 (1990).

3. Crystallization and preliminary X-ray diffraction studies of the engrailed homeodomain and of an engrailed homeodomain/DNA complex. Liu et al, Biochemical and Biophysical Research Communications, Vol. 171, No. 1, 1990, pages 257-259.

4. Changes of pH during Biomacromolecule Crystallization by Vapor Diffusion using Ammonium Sulfate as the Precipitant. Mikol et al, J. Appl. Cryst. (1989). 22, 155-161.

5. A Double Cell for Controlling Nucleation and Growth of Protein Crystals. Maria Przybylska, J. Appl. Cryst. (1989). 22, 115-118

6. Crystallization of butyrate kinase 2 from Thermotoga maritima mediated by vapor diffusion of acetic acid. Diao et al, Acta Cryst. (2003). D59, 1100±1102.

7. The preparation and crystallization of Fab fragments of a family of mouse esterolytic catalytic antibodies and their complexes with a transition-state analogue. Muranova et al, Acta Cryst. (2001). D57, 1192±1195

1. 蛋白质晶体的制备和分析。 Alexander McPherson，1982，John Wiley and Sons, Inc.，由 Krieger Publishing 印刷。第 4 章，结晶。

2. 大分子结晶的当前方法。亚历山大麦克弗森，欧元。 J.生化。 189, 1-23 (1990)。

3. engrailed homeodomain 和 engrailed homeodomain/DNA 复合物的结晶和初步 X 射线衍射研究。刘等人，生化和生物物理研究通讯，卷。 171，第 1 期，1990 年，第 257-259 页。

4. 使用硫酸铵作为沉淀剂通过蒸汽扩散进行生物大分子结晶过程中 pH 值的变化。 Mikol 等人，J. Appl。水晶。（1989 年）。 22, 155-161。

5. 用于控制蛋白质晶体成核和生长的双细胞。 Maria Przybylska, J. Appl.水晶。（1989 年）。 22、115-118

6. 乙酸蒸汽扩散介导的来自海栖热袍菌的丁酸激酶 2 的结晶。刁等人，Acta Cryst。（2003 年）。 D59，1100±1102。

7.小鼠酯水解催化抗体家族的Fab片段及其与过渡态类似物的复合物的制备和结晶。 Muranova 等人，Acta Cryst。（2001 年）。 D57, 1192±1195

Hampton蛋白结晶试剂盒Ammonium hydroxide

Products > Optimize Reagents > Optimize – Buffers > Ammonium hydroxide

Ammonium hydroxide

应用

Crystallization grade Ammonium hydroxide for altering drop and reservoir pH
用于改变液滴和油藏 pH 值的结晶级氢氧化铵

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Ammonium hydroxide

Synonyms: Ammonia aqueous, Ammonia water
CAS Number [1336-21-6]
NH₄OH
M_r 35.05
Beilstein Registry Number 3587154
EC Number 215-647-6
MDL Number MFCD00066650

See the pdf Using Volatile Buffers for a novel crystallization methodology using acetic acid and ammonium hydroxide to adjust drop pH and induce crystallization.

同义词：氨水、氨水
CAS 编号 [1336-21-6]
NH4OH
35.05 先生
贝尔斯坦注册号 3587154
欧盟编号 215-647-6
MDL 编号 MFCD00066650

有关使用乙酸和氢氧化铵调节液滴 pH 值并诱导结晶的新型结晶方法，请参阅 pdf 使用挥发性缓冲液。

Ammonium hydroxide

CAT NO

HR2-855

NAME

5.2 M Ammonium hydroxide

DESCRIPTION

10 mL

PRICE

$24.00

参考

1. Preparation and analysis of protein crystals. Alexander McPherson, 1982, John Wiley and Sons, Inc., printed by Krieger Publishing. Chapter 4, Crystallization.

2. Current approaches to macromolecular crystallization. Alexander McPherson, Eur. J. Biochem. 189, 1-23 (1990).

4. Changes of pH during Biomacromolecule Crystallization by Vapor Diffusion using Ammonium Sulfate as the Precipitant. Mikol et al, J. Appl. Cryst. (1989). 22, 155-161.

5. A Double Cell for Controlling Nucleation and Growth of Protein Crystals. Maria Przybylska, J. Appl. Cryst. (1989). 22, 115-118.

6. Crystallization of butyrate kinase 2 from Thermotoga maritima mediated by vapor diffusion of acetic acid. Diao et al, Acta Cryst. (2003). D59, 1100±1102.

1. 蛋白质晶体的制备和分析。 Alexander McPherson，1982，John Wiley and Sons, Inc.，由 Krieger Publishing 印刷。第 4 章，结晶。

2. 大分子结晶的当前方法。亚历山大麦克弗森，欧元。 J.生化。 189, 1-23 (1990)。

4. 使用硫酸铵作为沉淀剂通过蒸汽扩散进行生物大分子结晶过程中 pH 值的变化。 Mikol 等人，J. Appl。水晶。（1989 年）。 22, 155-161。

5. 用于控制蛋白质晶体成核和生长的双细胞。 Maria Przybylska, J. Appl.水晶。（1989 年）。 22, 115-118。

6. 乙酸蒸汽扩散介导的来自海栖热袍菌的丁酸激酶 2 的结晶。刁等人，Acta Cryst。（2003 年）。 D59，1100±1102。

7.小鼠酯水解催化抗体家族的Fab片段及其与过渡态类似物的复合物的制备和结晶。 Muranova 等人，Acta Cryst。（2001 年）。 D57，1192±1195。

Hampton蛋白结晶试剂盒BICINE Buffer

Products > Optimize Reagents > Optimize – Buffers > BICINE Buffer

BICINE Buffer

应用

Crystallization grade BICINE buffer for formulating screens or for optimization
用于配制屏幕或优化的结晶级 BICINE 缓冲液

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BICINE

Synonyms: N,N-Bis(2-hydroxyethyl)glycine
C₆H₁₃NO₄
M_r 163.17
CAS Number [150-25-4]
EC Number 205-755-1
Beilstein Registry Number 1769362
Merck 14,1201
RTECS MB9700000
MDL Number MFCD00004295
PubChem Substance ID 24891738
Purity ≥ 99.0%
pKa 8.3 at 20°C, 8.3 at 25°C

Useful pH Range 7.4-9.3
Titrate HR2-509 to useful pH range using NaOH
HR2-509 pH Range: 5.0 – 5.3 at 25°C
HR2-509 Measured Conductivity Range: 100.7 – 167.2 µS/cm at 25°C
HR2-509 Measured Refractive Index Range: 1.35769 – 1.35821 at 20°C

HR2-723 has been titrated to pH 9.0 using NaOH
HR2-723 Measured Conductivity Range: 23.9 – 25.0 mS/cm at 25°C
HR2-723 Measured Refractive Index Range: 1.36054 – 1.36122 at 20°C

同义词： N,N-双（2-羟乙基）甘氨酸
C6H13NO4
163.17先生
CAS 编号 [150-25-4]
欧盟编号 205-755-1
贝尔斯坦注册号 1769362
默克 14,1201
RTECS MB9700000
MDL 编号 MFCD00004295
PubChem 物质 ID 24891738
纯度≥99.0%
pKa 在 20°C 时为 8.3，在 25°C 时为 8.3

有用的 pH 范围 7.4-9.3
使用 NaOH 将 HR2-509 滴定至有用的 pH 范围
HR2-509 pH 范围：25°C 时为 5.0 – 5.3
HR2-509 测量电导率范围：25°C 时为 100.7 – 167.2 µS/cm
HR2-509 测量的折射率范围：20°C 时为 1.35769 – 1.35821

HR2-723 已使用 NaOH 滴定至 pH 9.0
HR2-723 测得的电导率范围：23.9 – 25.0 mS/cm，25°C
HR2-723 测量的折射率范围：20°C 时为 1.36054 – 1.36122

BICINE

CAT NO

HR2-509

NAME

1.0 M BICINE

DESCRIPTION

100 mL

PRICE

$104.00

CAT NO

HR2-723

NAME

1.0 M BICINE pH 9.0

DESCRIPTION

100 mL

PRICE

$104.00

参考

1. Crystallization and preliminary X-ray diffraction studies of a fungal hydrolase from Ophiostoma novo-ulmi. M. N. Isupov, A. A. Brindley, E. J. Hollingsworth, G. N. Murshudov, A. A. Vagin and J. A. Littlechild. Acta Cryst. (2004). D60, 1879-1882.

1. 来自 Ophiostoma novo-ulmi 的真菌水解酶的结晶和初步 X 射线衍射研究。 M. N. Isupov、A. A. Brindley、E. J. Hollingsworth、G. N. Murshudov、A. A. Vagin 和 J. A. Littlechild。晶体学报。（2004 年）。 D60，1879-1882。

Hampton蛋白结晶试剂盒BIS-TRIS Buffer

Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS Buffer

BIS-TRIS Buffer

应用

Crystallization grade BIS-TRIS buffer for formulating screens or for optimization
用于配制筛选或优化的结晶级 BIS-TRIS 缓冲液

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BIS-TRIS

Synonyms: 2,2-Bis(hydroxymethyl)-2,2′,2″”-nitrilotriethanol, or 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, or Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.
C₈H₁₉NO₅
M_r 209.24
CAS Number [6976-37-0]
EC Number 230-237-7
Beilstein Registry Number 2205275
MDL Number MFCD00002853
Purity ≥ 98.0%
pKa = 6.4 at 20°C
pKa = 6.5 at 25°C

HR2-781 is titrated to pH 5.5 using HCl
Measured Conductivity Range of HR2-781: 38.4 – 43.1 mS/cm at 25°C
Measured Refractive Index of HR2-781: 1.37069 – 1.37150 at 20°C

HR2-783 is titrated to pH 6.5 using HCl
Measured Conductivity Range of HR2-783: 22.8 – 28.7 mS/cm at 25°C
Measured Refractive Index of HR2-783: 1.36817 – 1.36926 at 20°C

同义词：2,2-双（羟甲基）-2,2′,2″”-次氮基三乙醇，或 2-双（2-羟乙基）氨基-2-（羟甲基）-1,3-丙二醇，或双（2- 羟乙基）氨基-三（羟甲基）甲烷。
C8H19NO5
209.24 先生
CAS 编号 [6976-37-0]
欧盟编号 230-237-7
贝尔斯坦注册号 2205275
MDL 编号 MFCD00002853
纯度≥98.0%
pKa = 6.4 在 20°C
pKa = 6.5 在 25°C

使用 HCl 将 HR2-781 滴定至 pH 5.5
HR2-781 的测量电导率范围：25°C 时为 38.4 – 43.1 mS/cm
HR2-781 的实测折射率：20°C 时为 1.37069 – 1.37150

HR2-783 使用 HCl 滴定至 pH 6.5
HR2-783 的测量电导率范围：22.8 – 28.7 mS/cm 在 25°C
HR2-783 的实测折射率：20°C 时为 1.36817 – 1.36926

Al ≤0.0005%
Ca ≤0.0005%
Cl ≤0.05%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Mg ≤0.0005%
NH₄ ≤0.05%
Na ≤0.005%
Pb ≤0.001%
SO₄ ≤0.05%
Zn ≤0.0005%

BIS-TRIS

CAT NO

HR2-781

NAME

1.0 M BIS-TRIS pH 5.5

DESCRIPTION

100 mL

PRICE

$169.00

CAT NO

HR2-783

NAME

1.0 M BIS-TRIS pH 6.5

DESCRIPTION

100 mL

PRICE

$169.00

参考

1. Atomic structure of the degraded procapsid particle of the bacteriophage G4: induced structural changes in the presence of calcium ions and functional implications. McKenna, R; Bowman, BR; Ilag, LL; Rossmann, MG; Fane, BA. J Mol Biol 256, 736- 750, 1996.

2. Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli. Montoya, G; Svensson, C; Luirink, J; Sinning, I. Proteins 28, 285- 288, 1997.

1. 噬菌体 G4 降解前衣壳颗粒的原子结构：在钙离子存在下诱导结构变化和功能影响。麦肯纳，R；鲍曼，BR；伊拉格，LL；罗斯曼，MG；法恩，文学士。分子生物学杂志 256, 736-750, 1996。

2.大肠杆菌信号识别颗粒对接蛋白FtsY的表达、结晶和初步X射线衍射研究。蒙托亚，G；斯文森，C；卢林克，J； Sinning, I. 蛋白质 28, 285-288, 1997。

Hampton蛋白结晶试剂盒Parabar 10312 (previously known as Paratone)

Products > Optimize Reagents > Optimize – Cryoprotectants > Parabar 10312 (previously known as Paratone)

Parabar 10312 (previously known as Paratone)

应用

Oil based cryoprotectant

特征

Synonyms used in the crystallographic literature: Paratone^® N, Paratone^® 8277, Infineum V8512
Oil based cryoprotectant

描述

Sorry, Parabar 10312 is no longer available. We’ve left this page up for reference only. See HR2-861 Santovac Cryo Oil as a possible substitute.

抱歉，Parabar 10312 不再可用。我们保留此页面仅供参考。请参阅 HR2-861 Santovac Cryo Oil 作为可能的替代品。

Parabar 10312 is a viscous cryoprotectant for both small and large molecule crystallography.¹

Mildly air-unstable, small molecule compounds can be coated with Parabar under an inert atmosphere. The Parabar protected crystal sample can be cryocooled in a chilled nitrogen gas stream. Parabar has also been used successfully as a cryoprotectant for biological macromolecule crystals. Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with Parabar can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

HR2-643 Parabar 10312 is no longer available and has been discontinued effective May 11, 2021.

HR2-862 Parabar 10312 is supplied as 5 ml in an amber glass vial with glass applicator rod in screw cap lid.

The viscosity of Parabar can be reduced to improve handling with fragile needle and blade crystals by mixing 50:50 with Paraffin Oil.

Parabar is a blend of polyisobutylenes with a Staudinger molecular weight of about 20,000 and contains about 20% polymer.

Refractive index 1.48487 at 20°C.

Kinematic viscosity 641 cSt at 100°C. Kinematic viscosity ratio 2.05 at 100°C.

Clear to slightly hazy is appearance.

Synonyms used in the crystallographic literature: Paratone^® N, Paratone^® 8277, Infineum V8512.

Paratone^® is a registered trademark of Chevron Corporation.

Parabar 10312 是一种粘性冷冻保护剂，适用于小分子和大分子晶体学。 1

可以在惰性气氛下用 Parabar 包覆对空气轻微不稳定的小分子化合物。 Parabar 保护的晶体样品可以在冷冻氮气流中进行低温冷却。 Parabar 也已成功用作生物大分子晶体的冷冻保护剂。在晶体安装在低温回路中后，在低温晶体学期间用于置换和减少晶体上的水（母液、试剂）量。用 Parabar 涂覆晶体可以最大限度地减少晶体的蒸发，并减少空气（氧气）向晶体的暴露和缓慢扩散。

HR2-643 Parabar 10312 不再可用，自 2021 年 5 月 11 日起停产。

HR2-862 Parabar 10312 以 5 ml 装在琥珀色玻璃小瓶中，螺旋盖盖中带有玻璃涂抹棒。

通过将 50:50 与石蜡油混合，可以降低 Parabar 的粘度，以改善易碎针状和刀片状晶体的处理。

Parabar 是聚异丁烯的混合物，Staudinger 分子量约为 20,000，含有约 20% 的聚合物。

20°C 时的折射率为 1.48487。

100°C 时运动粘度为 641 cSt。 100°C 时运动粘度比为 2.05。

清晰到略微朦胧的是外观。

晶体学文献中使用的同义词：Paratone® N、Paratone® 8277、润英联 V8512。

Paratone® 是雪佛龙公司的注册商标。

Parabar 10312

参考

1. H. Hope, Cryocrystallography of biological macromolecules: a generally applicable method. Acta Cryst. (1988) B44, 22-26.

2. Structure of the ligand-binding domain (LBD) of human androgen receptor in complex with a selective modulator LGD2226. F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi and T. Jiang. Acta Cryst. (2006). F62, 1067-1071.

3. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126.

4. S. Parkin and H. Hope, J. Appl. Cryst. (1998) pages 945-953.

1. H. Hope，生物大分子的低温晶体学：一种普遍适用的方法。晶体学报。 (1988) B44，22-26。

2. 与选择性调节剂 LGD2226 复合的人雄激素受体的配体结合域 (LBD) 的结构。 F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi 和 T. Jiang。晶体学报。（2006 年）。 F62, 1067-1071。

3. H.霍普，安努。牧师生物物理学。化学。 1990 年 19：107-126。

4. S. Parkin 和 H. Hope，J. Appl。水晶。 (1998) 第 945-953 页。

Hampton蛋白结晶试剂盒BIS-TRIS propane Buffer

Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS propane Buffer

BIS-TRIS propane Buffer

应用

Crystallization grade BIS-TRIS propane pH 7.0 for formulating screens or for optimization
结晶级 BIS-TRIS 丙烷 pH 7.0，用于配制筛选或优化

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BIS-TRIS propane

Synonyms: 1,3-Bis[tris(hydroxymethyl)methylamino]propane
C₁₁H₂₆N₂O₆
CH₂[CH₂NHC(CH₂OH)₃]₂
M_r 282.34
CAS Number [64431-96-5]
EC Number 264-899-3
Beilstein Registry Number 1786109
MDL Number MFCD00004689
PubChem Substance ID 24891934
Purity ≥ 99.0%
pKa1 = 6.8 at 25°C
pKa2 = 9.0 at 25°C

HR2-795 is titrated to pH 7.0 using HCl
Measured Conductivity Range: 41.9 – 43.5 mS/cm at 25°C
Measured Refractive Index Range: 1.39110 – 1.39222 at 20°C

别名：1,3-双[三(羟甲基)甲氨基]丙烷
C11H26N2O6
CH2[CH2NHC(CH2OH)3]2
282.34 先生
CAS 编号 [64431-96-5]
欧盟编号 264-899-3
贝尔斯坦注册号 1786109
MDL 编号 MFCD00004689
PubChem 物质 ID 24891934
纯度≥99.0%
pKa1 = 6.8 在 25°C
pKa2 = 9.0 在 25°C

HR2-795 使用 HCl 滴定至 pH 7.0
测得的电导率范围：25°C 时 41.9 – 43.5 mS/cm
测得的折射率范围：20°C 时为 1.39110 – 1.39222

BIS-TRIS propane

CAT NO

HR2-795

NAME

1.0 M BIS-TRIS propane pH 7.0

DESCRIPTION

100 mL

PRICE

$108.00

参考

1. Two-fold symmetry of crystalline DNA-EcoRI endonuclease recognition complexes. Grable, J; Frederick, CA; Samudzi, C; Jen-Jacobson, L; Lesser, D; Greene, P; Boyer, HW; Itakura, K; Rosenberg, JM. J Biomol Struct Dyn 1, 1149- 1160, 1984.

2. Non-native Local Interactions in Protein Folding and Stability: Introducing a Helical Tendency in the AII beta-Sheet alpha-Spectrin SH3 Domain. Prieto, J; Wilmans, M; Jimenez, MA; Rico, M; Serrano, L. J Mol Biol 268, 760- 778, 1997.

1. 结晶 DNA-EcoRI 核酸内切酶识别复合物的双重对称性。格拉布尔，J；加利福尼亚州弗雷德里克；萨穆兹，C；仁-雅各布森，L；较小，D；格林，P；博耶，HW；板仓，K；罗森伯格，JM。 J Biomol Struct Dyn 1, 1149-1160, 1984。

2. 蛋白质折叠和稳定性中的非天然局部相互作用：在 AII β-片 α-Spectrin SH3 域中引入螺旋趋势。普列托，J；威尔曼斯，M；马萨诸塞州希门尼斯；里科，M； Serrano, L. J Mol Biol 268, 760-778, 1997。

Hampton蛋白结晶试剂盒Citric acid Buffer

Products > Optimize Reagents > Optimize – Buffers > Citric acid Buffer

Citric acid Buffer

应用

Crystallization grade Citric acid buffer for formulating screens or for optimization
用于配制筛选或优化的结晶级柠檬酸缓冲液

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 Citric acid

Synonyms: Citric acid anhydrous
C₆H₈O₇
HOC(COOH)(CH₂COOH)₂
M_r 192.13
CAS Number [77-92-9]
EC Number 201-069-1
Beilstein Registry Number 782061
Merck 14,2326
RTECS GE7350000
MDL Number MFCD00011669
PubChem Substance ID 24856611
Purity ≥ 99.5%
Useful pH range of citric acid as a buffer 2.2 – 6.5
pKa1 = 3.13
pKa2 = 4.76
pKa3 = 6.4

HR2-757 is titrated to pH 3.5 at 25 degrees Celsius using NaOH
Measured Conductivity Range: 28.7 – 33.3 mS/cm at 25°C
Measured Refractive Index Range: 1.36236 – 1.36329 at 20°C

别名：无水柠檬酸
C6H8O7
HOC(COOH)(CH2COOH)2
192.13 先生
CAS 编号 [77-92-9]
欧盟编号 201-069-1
贝尔斯坦注册号 782061
默克 14,2326
RTECS GE7350000
MDL 编号 MFCD00011669
PubChem 物质 ID 24856611
纯度≥99.5%
柠檬酸作为缓冲液的有用 pH 值范围 2.2 – 6.5
pKa1 = 3.13
pKa2 = 4.76
pKa3 = 6.4

HR2-757 在 25 摄氏度下使用 NaOH 滴定至 pH 3.5
测得的电导率范围：25°C 时 28.7 – 33.3 mS/cm
测得的折射率范围：20°C 时为 1.36236 – 1.36329

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni ≤0.0005%
Oxalate (C₂O₄) ≤0.05%
Pb ≤0.0005%
PO₄ ≤0.0005%
SO₄ ≤0.002%
Sr ≤0.0005%
Tartrate (C₄H₄O₆) ≤0.2%
Zn ≤0.0005%

Citric acid

CAT NO

HR2-757

NAME

1.0 M Citric acid pH 3.5

DESCRIPTION

100 mL

PRICE

$44.00

参考

1. Crystallization and preliminary X-ray structure solution of Rhizomucor miehei aspartic proteinase. Jia, Z; Vandonselaar, M; Schneider, P; Quail, JW. Acta Crystallogr D 51, 243- 244, 1995.

1.米黑根毛霉天冬氨酸蛋白酶的结晶和初步X射线结构解。贾，Z；范登塞拉，M；施耐德，P；鹌鹑，JW。晶体学学报 D 51, 243-244, 1995。

Hampton蛋白结晶试剂盒Citric acid BIS-TRIS propane Buffer

Products > Optimize Reagents > Optimize – Buffers > Citric acid BIS-TRIS propane Buffer

Citric acid BIS-TRIS propane Buffer

应用

Crystallization grade CBTP buffer for formulating screens or for optimization
用于配制筛选或优化的结晶级 CBTP 缓冲液

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

Citrate BIS-TRIS propane (CBTP) is a buffer system useful across pH 2.5 to 9.5.

Using CBTP one can use a single buffer system to screen pH 2.5 to 9.5 by varying the ratio of Citric acid to BIS-TRIS propane. Although the two buffer reagents are made available separately for convenient, individual replacement, they are designed to be used together, with the supplied CBTP buffer titration table to create a crystallization buffer system covering pH 2.5 to 9.5.

HR2-831 Measured pH range: 1.2 – 1.5 at 25°C
HR2-831 Measured Conductivity Range: 7.7 – 8.7 mS/cm at 25°C

HR2-833 Measured pH range: 11.2 – 11.5 at 25°C
HR2-833 Measured Conductivity Range: 298 – 317 µS/cm at 25°C
HR2-833 Measured Refractive Index Range: 1.38000 – 1.38076 at 20°C

柠檬酸盐 BIS-TRIS 丙烷 (CBTP) 是一种适用于 pH 2.5 至 9.5 的缓冲系统。

使用 CBTP，可以使用单一缓冲系统通过改变柠檬酸与 BIS-TRIS 丙烷的比例来筛选 pH 2.5 至 9.5。虽然这两种缓冲试剂是分开提供的，以便于单独更换，但它们被设计为一起使用，与提供的 CBTP 缓冲液滴定表一起创建一个涵盖 pH 2.5 至 9.5 的结晶缓冲系统。

HR2-831 测得的 pH 值范围：25°C 时为 1.2 – 1.5
HR2-831 测量电导率范围：25°C 时 7.7 – 8.7 mS/cm

HR2-833 测得的 pH 范围：25°C 时为 11.2 – 11.5
HR2-833 测量的电导率范围：298 – 317 µS/cm，25°C
HR2-833 测量的折射率范围：20°C 时为 1.38000 – 1.38076

Citric acid

CAT NO

HR2-831

NAME

1.0 M Citric acid

DESCRIPTION

100 mL

PRICE

$45.00

CAT NO

HR2-833

NAME

1.0 M BIS-TRIS propane

DESCRIPTION

100 mL

PRICE

$162.00

Hampton蛋白结晶试剂盒HEPES Buffer

Products > Optimize Reagents > Optimize – Buffers > HEPES Buffer

HEPES Buffer

应用

Crystallization grade HEPES buffer for formulating screens or for optimization
用于配制筛选或优化的结晶级 HEPES 缓冲液

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 HEPES

Synonyms: 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid or 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
C₈H₁₈N₂O₄S
M_r 238.31
CAS Number [7365-45-9]
EC Number 230-907-9
Beilstein Registry Number 883043
Merck 14,4654
RTECS TL6809000
MDL Number MFCD00006158
PubChem Substance ID 24895616
Purity ≥ 99.5%
pKa 7.5 at 25°C

Useful pH range of HEPES buffer 6.8 – 8.2

别名：2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid or 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-Hydroxyethyl)piperazine-N’-(2-ethanesulfonic 酸）
C8H18N2O4S
238.31 先生
CAS 编号 [7365-45-9]
欧盟编号 230-907-9
贝尔斯坦注册号 883043
默克 14,4654
RTECS TL6809000
MDL 编号 MFCD00006158
PubChem 物质 ID 24895616
纯度≥99.5%
pKa 7.5 在 25°C

HEPES 缓冲液的有用 pH 范围 6.8 – 8.2

Titrate HR2-585 to the desired pH between 6.8 – 8.2 using NaOH
HR2-585 Measured pH Range: 5.4 – 5.5 at 25°C
HR2-585 Measured Conductivity Range: 160.1 – 236.9 μS/cm at 25°C
HR2-585 Measured Refractive Index Range: 1.37093 – 1.37131 at 20°C

HR2-785 is titrated to pH 7.0 using NaOH
HR2-785 Measured Conductivity Range: 5.8 – 7.0 mS/cm at 25°C
HR2-785 Measured Refractive Index Range: 1.37133 – 1.37176 at 20°C

HR2-729 is titrated to pH 7.5 using NaOH
HR2-729 Measured Conductivity Range: 11.6 – 13.6 mS/cm at 25°C
HR2-729 Measured Refractive Index Range: 1.37177 – 1.37290 at 20°C

Insoluble Matter 0.01% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 1 ppm max
As 5 ppm max
Cu 5 ppm max
Fe 5 ppm max
Pb 5 ppm max
SO₄ 0.005% max

CAT NO

HR2-585

NAME

1.0 M HEPES

DESCRIPTION

100 mL

PRICE

$113.00

CAT NO

HR2-785

NAME

1.0 M HEPES pH 7.0

DESCRIPTION

100 mL

PRICE

$113.00

CAT NO

HR2-729

NAME

1.0 M HEPES pH 7.5

DESCRIPTION

100 mL

PRICE

$113.00

参考

1. Crystallization and preliminary X-ray crystallographic studies of native elastase from North Atlantic salmon (Salmo salar). Berglund, GI; Snalas, AO; Hansen, LK. Acta Crystallogr D 51, 393- 394, 1995.

1. 来自北大西洋鲑鱼 (Salmo salar) 的天然弹性蛋白酶的结晶和初步 X 射线晶体学研究。伯格伦德，GI；斯纳拉斯，AO；汉森，LK。晶体学学报 D 51, 393-394, 1995。

Hampton蛋白结晶试剂盒HEPES sodium Buffer

Products > Optimize Reagents > Optimize – Buffers > HEPES sodium Buffer

HEPES sodium Buffer

应用

Crystallization grade HEPES sodium buffer for formulating screens or for optimization
用于配制筛选或优化的结晶级 HEPES 钠缓冲液

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述HEPES sodium

Synonyms: HEPES sodium salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, or N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
C₈H₁₇N₂NaO₄S
M_r 260.29
CAS [75277-39-3]
EC Number 278-169-7
MDL Number MFCD00036463
PubChem Substance ID 24895780
pKa 7.5 at 25°C
Useful pH Range of HEPES sodium: 6.6 – 8.2

别名：HEPES钠盐，或4-（2-羟乙基）哌嗪-1-乙磺酸钠盐，或N-（2-羟乙基）哌嗪-N’-（2-乙磺酸）钠盐
C8H17N2NaO4S
260.29 先生
CAS [75277-39-3]
欧盟编号 278-169-7
MDL 编号 MFCD00036463
PubChem 物质 ID 24895780
pKa 7.5 在 25°C
HEPES钠的有用pH范围：6.6 – 8.2

HR2-733 is titrated to pH 7.5 using HCl
HR2-733 measured Conductivity range: 41.7 – 43.4 mS/cm at 25°C
HR2-733 measured Refractive Index range: 1.37577 – 1.37766 at 20°C

HR2-577 is the free base of HEPES sodium and is to be titrated to the desired pH using HCl
HR2-577 measured pH Range: 10.2 – 12.1 at 25°C
HR2-577 measured Conductivity range: 25.9 – 27.2 mS/cm at 25°C
HR2-577 measured Refractive Index range: 1.37097 – 1.37298 at 20°C

HR2-931-01 – 1.0 M HEPES sodium pH 6.8 is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.

使用 HCl 将 HR2-733 滴定至 pH 7.5
HR2-733 测量的电导率范围：25°C 时 41.7 – 43.4 mS/cm
HR2-733 测量的折射率范围：20°C 时为 1.37577 – 1.37766

HR2-577 是 HEPES 钠的游离碱，使用 HCl 滴定至所需的 pH 值
HR2-577 测量的 pH 范围：25°C 时为 10.2 – 12.1
HR2-577 测量的电导率范围：25.9 – 27.2 mS/cm 在 25°C
HR2-577 测量的折射率范围：20°C 时为 1.37097 – 1.37298

HR2-931-01 – 1.0 M HEPES 钠 pH 6.8 是 Custom Shop 试剂，用于复制和优化与 Silver Bullets 试剂盒配合使用的结晶试剂。

CAT NO

HR2-577

NAME

1.0 M HEPES sodium

DESCRIPTION

100 mL

PRICE

$133.00

CAT NO

HR2-733

NAME

1.0 M HEPES sodium pH 7.5

DESCRIPTION

100 mL

PRICE

$133.00

参考

1. Crystallization and preliminary X-ray characterization of GM2-activator protein. C. S. Wright and S.-C. Li. Acta Cryst. (1997). D53, 211-212.

1. GM2 激活蛋白的结晶和初步 X 射线表征。 C. S. 赖特和 S.-C。李。晶体学报。 (1997)。 D53，211-212。

Hampton蛋白结晶试剂盒Imidazole Buffer/HR2-573/HR2-819

Products > Optimize Reagents > Optimize – Buffers > Imidazole Buffer

Imidazole Buffer

应用

Crystallization grade Imidazole for formulating screens or for optimization
用于配制筛选或优化的结晶级咪唑

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 Imidazole

Synonyms: 1,3-Diaza-2,4-cyclopentadiene or Glyoxaline
C₃H₄N₂
M_r 68.08
CAS Number [288-32-4]
EC Number 206-019-2
Beilstein Registry Number 103853
Merck 14,4912
RTECS NI3325000
MDL Number MFCD00005183
PubChem Substance ID 24880372
Purity ≥ 99.5%
UN Number 2923 8/PG 3

pKa = 6.95
The useful pH range of an Imidazole can be 6.2 – 7.8

HR2-819 is titrated to pH 7.0 using HCl
HR2-819 Measured Conductivity Range: 45.4 – 50.4 mS/cm at 25°C
HR2-819 Measured Refractive Index Range: 1.34836 – 1.34904 at 20°C

Titrate HR2-573 to the desired pH using HCl
HR2-573 Measured pH Range: 10.5 – 10.7 at 25°C
HR2-573 Measured Conductivity Range: 71.1 – 101.5 µS/cm at 25°C
HR2-573 Measured Refractive Index Range: 1.34420 – 1.34440 at 20°C

同义词：1,3-Diaza-2,4-cyclopentadiene 或 Glyoxaline
C3H4N2
68.08 先生
CAS 编号 [288-32-4]
欧盟编号 206-019-2
贝尔斯坦注册号 103853
默克 14,4912
RTECS NI3325000
MDL 编号 MFCD00005183
PubChem 物质 ID 24880372
纯度≥99.5%
联合国编号 2923 8/PG 3

pKa = 6.95
咪唑的有用 pH 范围可以是 6.2 – 7.8

HR2-819 使用 HCl 滴定至 pH 7.0
HR2-819 测得的电导率范围：25°C 时为 45.4 – 50.4 mS/cm
HR2-819 测量的折射率范围：20°C 时为 1.34836 – 1.34904

使用 HCl 将 HR2-573 滴定至所需的 pH 值
HR2-573 测得的 pH 范围：25°C 时 10.5 – 10.7
HR2-573 测得的电导率范围：25°C 时为 71.1 – 101.5 µS/cm
HR2-573 测量的折射率范围：20°C 时为 1.34420 – 1.34440

Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Mg ≤0.0005%
Na ≤0.005%
Ni ≤0.0005%
Pb ≤0.0005%
SO₄ ≤0.005%
Zn ≤0.0005%

CAT NO

HR2-573

NAME

1.0 M Imidazole

DESCRIPTION

100 mL

PRICE

$93.00

CAT NO

HR2-819

NAME

1.0 M Imidazole pH 7.0

DESCRIPTION

100 mL

PRICE

$111.00

参考

1. Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions. X. Qiu and C. A. Janson. Acta Cryst. (2004). D60, 1545-1554 [doi:10.1107/S0907444904015422].

2. Protein crystallization by surface entropy reduction: optimization of the SER strategy. D. R. Cooper, T. Boczek, K. Grelewska, M. Pinkowska, M. Sikorska, M. Zawadzki and Z. Derewenda. Acta Cryst. (2007). D63, 636-645 [doi:10.1107/S0907444907010931].

1. apo 酰基载体蛋白的结构和通过金属离子设计蛋白质结晶的建议。 X. 邱和 C. A. Janson。晶体学报。（2004 年）。 D60, 1545-1554 [doi:10.1107/S0907444904015422]。

2. 表面熵减少的蛋白质结晶：SER 策略的优化。 D. R. Cooper、T. Boczek、K. Grelewska、M. Pinkowska、M. Sikorska、M. Zawadzki 和 Z. Derewenda。晶体学报。（2007 年）。 D63, 636-645 [doi:10.1107/S0907444907010931]。

MES monohydrate Buffer/HR2-587 0.5 M MES monohydrate/HR2-787 1.0 M MES monohydrate pH 6.5

Products > Optimize Reagents > Optimize – Buffers > MES monohydrate Buffer

MES monohydrate Buffer

CAT NO

HR2-587

NAME

0.5 M MES monohydrate

DESCRIPTION

100 mL

PRICE

$141.00

CAT NO

HR2-787

NAME

1.0 M MES monohydrate pH 6.5

DESCRIPTION

100 mL

PRICE

$141.00

应用

Crystallization grade MES monohydrate for formulating screens or for optimization
用于配制筛选或优化的结晶级 MES 一水合物

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 MES monohydrate

Synonyms: MES or 2-(N-morpholino)ethanesulfonic acid or 4-morpholineethanesulfonic acid monohydrate
C₆H₁₃NO₄S • H₂O
M_r 213.25
CAS Number [145224-94-8]
EC Number 224-632-3
Beilstein Registry Number 329824236
RTECS QE3479500
MDL Number MFCD00149409
PubChem Substance ID 24885996
Purity ≥ 99.0%
pKa 6.1 at 25°C

Solutions are stable at 2-8°C for months. Autoclaving is not recommended for any sulfonic acid buffer. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown. When MES solutions are stored at room temperature, they can turn yellow within six months.

Useful pH range of MES 5.2 – 7.1

Titrate HR2-587 to the desired pH using NaOH
HR2-587 Measured pH Range: 3.2 – 3.4 at 25°C
HR2-587 Measured Conductivity Range: 166.4 – 2440 µS/cm at 25°C
HR2-587 Measured Refractive Index Range: 1.34655 – 1.34731 at 20°C

HR2-787 has been titrated to pH 6.5 using NaOH
HR2-787 Measured Conductivity Range: 19.2 – 25.6 mS/cm at 25°C
HR2-787 Measured Refractive Index Range: 1.36113 – 1.36251 at 20°C

HR2-943-07 – MES monohydrate pH 5.8 – 1.0 M solution is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.

同义词：MES或2-(N-吗啉代)乙磺酸或4-吗啉乙磺酸一水合物
C6H13NO4S • H2O
213.25 先生
CAS 编号 [145224-94-8]
欧盟编号 224-632-3
贝尔斯坦注册号 329824236
RTECS QE3479500
MDL 编号 MFCD00149409
PubChem 物质 ID 24885996
纯度≥99.0%
pKa 6.1 在 25°C

溶液在 2-8°C 下可稳定数月。不建议对任何磺酸缓冲液进行高压灭菌。当 MES 溶液经过高压灭菌时，它们会变黄（尽管 pH 值不会发生可测量的变化）。黄色分解产物的身份不明。当 MES 溶液在室温下储存时，它们会在六个月内变黄。

MES 5.2 – 7.1 的有用 pH 范围

使用 NaOH 将 HR2-587 滴定至所需的 pH 值
HR2-587 测得的 pH 范围：25°C 时为 3.2 – 3.4
HR2-587 测得的电导率范围：25°C 时为 166.4 – 2440 µS/cm
HR2-587 测量的折射率范围：20°C 时为 1.34655 – 1.34731

HR2-787 已使用 NaOH 滴定至 pH 6.5
HR2-787 测量的电导率范围：25°C 时为 19.2 – 25.6 mS/cm
HR2-787 测量的折射率范围：20°C 时为 1.36113 – 1.36251

HR2-943-07 – MES 一水合物 pH 5.8 – 1.0 M 溶液是 Custom Shop 试剂，用于重现和优化用于 Silver Bullets 试剂盒的结晶试剂。

Impurities:
Endotoxin ≤ 50 EU/g
Yeast and Mold ≤ 100 CFU/g
Bioburden ≤ 100 CFU/g
Al ≤ 5.0 ppm
As ≤ 3.0 ppm
Ba ≤ 5.0 ppm
Bi ≤ 5.0 ppm
Ca ≤ 20.0 ppm
Cd ≤ 5.0 ppm
Co ≤ 5.0 ppm
Cr ≤ 5.0 ppm
Cu ≤ 5.0 ppm
Fe ≤ 5.0 ppm
Li ≤ 5.0 ppm
Mg ≤ 5.0 ppm
Mn ≤ 5.0 ppm
Mo ≤ 5.0 ppm
Na ≤ 20.0 ppm
Ni ≤ 5.0 ppm
Pb ≤ 5.0 ppm
Sb ≤ 5.0 ppm
Zn ≤ 5.0 ppm

参考

1. Crystal structures of mutant Pseudomonas aeruginosa p-hydroxybenzoate hydroxylases: the Tyr201Phe, Tyr385Phe, and Asn300Asp variants. Lah, MS; Palfey, BA; Schreuder, HA; Ludwig, ML. Biochemistry 33, 1555- 1564, 1994.

1. 突变铜绿假单胞菌对羟基苯甲酸羟化酶的晶体结构：Tyr201Phe、Tyr385Phe 和 Asn300Asp 变体。拉，女士；帕尔菲，文学士；施罗德，HA；路德维希，ML。生物化学 33, 1555-1564, 1994。

Hampton蛋白结晶试剂盒Perfluoropolyether Cryo Oil/HR2-814

Products > Optimize Reagents > Optimize – Cryoprotectants > Perfluoropolyether Cryo Oil

Perfluoropolyether Cryo Oil全氟聚醚冷冻油

CAT NO

HR2-814

NAME

Perfluoropolyether Cryo Oil

DESCRIPTION

1 mL

PRICE

$33.00

应用

Oil based cryoprotectant
油基防冻剂

特征

Perfluoropolyether cryo oil
Low viscosity
Low surface tension
Cryoprotectant for soluble and membrane protein crystals
全氟聚醚冷冻油
低粘度
低表面张力
可溶性和膜蛋白晶体的冷冻保护剂

描述

Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with oil can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

Perfluoropolyether Cryo Oil offers low viscosity, extremely low vapor pressure, low surface tension, excellent chemical inertness and excellent thermal stability.

Dip the mounted crystal into the oil to displace surface water. Or add a small amount of Perfluoropolyether to the crystallization drop before mounting the crystal. After adding the Perfluoropolyether, mount the crystal using a Mounted Cryoloop. Withdraw the mounted crystal from the drop and the Perfluoropolyether will coat the mounted crystal. Cryogenically cool the mounted crystal.

在晶体安装在低温回路中后，在低温晶体学期间用于置换和减少晶体上的水（母液、试剂）量。用油涂抹晶体可以最大限度地减少晶体的蒸发并减少空气（氧气）向晶体的暴露和缓慢扩散。

全氟聚醚冷冻油具有低粘度、极低蒸气压、低表面张力、优异的化学惰性和优异的热稳定性。

将安装的晶体浸入油中以置换表面水。或者在安装晶体之前在结晶液滴中加入少量的全氟聚醚。添加全氟聚醚后，使用 Mounted Cryoloop 安装晶体。从液滴中取出已镶嵌的晶体，全氟聚醚将覆盖已镶嵌的晶体。低温冷却安装的晶体。

参考

1. Structure of the ‘open form’ of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 Å resolution. C.E. Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.

2. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126.
1. 构巢曲霉 3-脱氢喹酸合酶“开放形式”的结构，分辨率为 1.7 Å。 C.E.尼科尔斯，A.R. 霍金斯和 D.K. 斯塔默萨。晶体学报 D 部分，第 60 卷，第 5 部分，第 971-973 页，2004 年 5 月。

2. H.希望，安努。牧师生物物理学。化学。 1990 年 19：107-126。

Hampton蛋白结晶试剂盒Sodium cacodylate trihydrate Buffer/HR2-575/HR2-737

Products > Optimize Reagents > Optimize – Buffers > Sodium cacodylate trihydrate Buffer

Sodium cacodylate trihydrate Buffer二甲胂酸钠三水合物

CAT NO

HR2-575

NAME

1.0 M Sodium cacodylate trihydrate

DESCRIPTION

100 mL

PRICE

$244.00

CAT NO

HR2-737

NAME

1.0 M Sodium cacodylate trihydrate pH 6.5

DESCRIPTION

100 mL

PRICE

$244.00

应用

Crystallization grade Sodium cacodylate trihydrate buffer for formulating screens or for optimization
结晶级二甲胂酸钠三水合物缓冲液，用于配制筛选或优化

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Sodium cacodylate trihydrate

Synonyms: Cacodylic acid sodium salt trihydrate or Dimethylarsinic acid sodium salt or Dimethylarsonic acid sodium salt
C₂H₆AsNaO₂• 3H₂O
(CH₃)₂AsO₂Na • 3H₂O
M_r 214.03
CAS Number [6131-99-3]
EC Number 204-708-2
Beilstein Registry Number 3702348
Merck 14,8595
RTECS CH7890000
MDL Number MFCD00149079
PubChem Substance ID 24892242
Purity ≥ 98.0%
UN Number UN 1688 6.1/PG 2
pKa 6.3 at 25°C

Useful pH Range of a Sodium cacodylate trihydrate buffer is 5.0 – 7.4

HR2-737 is titrated to pH 6.5 using HCl
HR2-737 Measured Conductivity Range: 43.4 – 47.1 mS/cm at 25°C
HR2-737 Measured Refractive Index Range: 1.35633 – 1.35711 at 20°C

Titrate HR2-575 to the desired pH using HCl
HR2-575 Measured pH Range: 8.4 – 8.5 at 25°C
HR2-575 Measured Conductivity Range: 33.7 – 36.5 mS/cm at 25°C
HR2-575 Measured Refractive Index Range: 1.35556 – 1.35635 at 20°C

别名：二甲胂酸钠盐三水合物或二甲基胂酸钠盐或二甲基胂酸钠盐
C2H6AsNaO2 • 3H2O
(CH3)2AsO2Na • 3H2O
214.03 先生
CAS 编号 [6131-99-3]
欧盟编号 204-708-2
贝尔斯坦注册号 3702348
默克 14,8595
RTECS CH7890000
MDL 编号 MFCD00149079
PubChem 物质 ID 24892242
纯度≥98.0%
联合国编号 UN 1688 6.1/PG 2
pKa 6.3 在 25°C

二甲胂酸钠三水合物缓冲液的有用 pH 范围为 5.0 – 7.4

使用 HCl 将 HR2-737 滴定至 pH 6.5
HR2-737 测得的电导率范围：25°C 时 43.4 – 47.1 mS/cm
HR2-737 测量的折射率范围：20°C 时为 1.35633 – 1.35711

使用 HCl 将 HR2-575 滴定至所需的 pH 值
HR2-575 测量的 pH 范围：25°C 时 8.4 – 8.5
HR2-575 测得的电导率范围：25°C 时 33.7 – 36.5 mS/cm
HR2-575 测量的折射率范围：20°C 时为 1.35556 – 1.35635

参考

1. The high-resolution crystal structure of a parallel-stranded guanine tetraplex. Laughlan, G; Murchie, AI; Norman, DG; Moore, MH; Moody, PC; Lilley, DM; Luisi, B. Science 265, 520- 524, 1994.

2. Get phases from arsenic anomalous scattering: de novo SAD phasing of two protein structures crystallized in cacodylate buffer. Liu X, Zhang H, Wang XJ, Li LF, Su XD. PLoS One. 2011;6(9):e24227. Epub 2011 Sep 2.

1. 平行链鸟嘌呤四链体的高分辨率晶体结构。劳克兰，G；默奇，人工智能；诺曼，总干事；摩尔，MH；穆迪，个人电脑；莉莉，DM； Luisi, B. Science 265, 520-524, 1994。

2. 从砷异常散射中获取相位：在二甲胂酸盐缓冲液中结晶的两种蛋白质结构的从头 SAD 相位。刘 X，张 H，王 XJ，李 LF，苏 XD。公共科学图书馆一号。 2011;6(9):e24227。 Epub 2011 年 9 月 2 日。

Hampton蛋白结晶试剂盒Santovac Cryo Oil/HR2-861

Products > Optimize Reagents > Optimize – Cryoprotectants > Santovac Cryo Oil

Santovac Cryo Oil

CAT NO

HR2-861

NAME

Santovac Cryo Oil

DESCRIPTION

1 mL

PRICE

$26.00

应用

Oil based cryoprotectant
油基防冻剂

特征

Low viscosity
Low surface tension
Cryoprotectant for soluble and membrane protein crystals
Not miscible with most solvents
Non toxic, non penetrating cryoprotectant
低粘度
低表面张力
可溶性和膜蛋白晶体的冷冻保护剂
不与大多数溶剂混溶
无毒、非渗透性冷冻保护剂

描述

Santovac Cryo Oil offers low viscosity, incredibly low vapor pressure, low surface tension, excellent chemical inertness and excellent thermal stability.

Dip the mounted crystal into the oil to displace surface water. Or add a small amount of Santovac to the crystallization drop before mounting the crystal. After adding the Santovac, mount the crystal using a Mounted Cryoloop. Withdraw the mounted crystal from the drop and the Santovac will coat the mounted crystal. Cryogenically cool the mounted crystal.

Santovac (Santovac 5) is a five ring polyphenyl ether (PPE) with very low vapor pressure and low reactivity. The oil is highly resistant to oxidation. The only elements in the pure polyphenyl ether are oxygen, hydrogen and carbon.

The fluid is chemically stable, non-corrosive, safe and non-toxic at normal operating temperatures.

Santovac is not miscible with water, paraffin oil, silicon oil, alkanes, or Parabar 10312 (Paratone). Santovac is miscible with acetone. Shelf life 5 years.

Santovac^® is a trademark of Santovac^® Fluids.

Santovac Cryo Oil 具有低粘度、极低的蒸气压、低表面张力、出色的化学惰性和出色的热稳定性。

将安装的晶体浸入油中以置换表面水。或者在安装晶体之前将少量的 Santovac 添加到结晶液滴中。添加 Santovac 后，使用 Mounted Cryoloop 安装晶体。从水滴中取出已镶嵌的水晶，Santovac 将覆盖已镶嵌的水晶。低温冷却安装的晶体。

Santovac (Santovac 5) 是一种五环聚苯醚 (PPE)，具有极低的蒸气压和低反应性。该油具有很强的抗氧化性。纯聚苯醚中唯一的元素是氧、氢和碳。

该流体在正常工作温度下化学性质稳定、无腐蚀性、安全无毒。

Santovac 与水、石蜡油、硅油、烷烃或 Parabar 10312 (Paratone) 不混溶。 Santovac 可与丙酮混溶。保质期5年。

Santovac® 是 Santovac® Fluids 的商标。

Santovac Cryo Oil

支持材料

HR2-861 Santovac Cryo Oil User Guide HR2-861 Santovac Cryo Oil SDS

参考

1. Structure of the ‘open’ form of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 Å resolution from crystal grown following enzyme turnover. C.E. Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.

2. Crystal Structure of the Caspase Activator Human Granzyme B, a Proteinase Highly Specific for an Asp-P1 Residue. E. Estebanez-Perpina et al. Biol. Chem., Vol. 381, pp. 1203-1214, December 2000.

1. 构巢曲霉 3-脱氢喹酸合酶“开放”形式的结构，分辨率为 1.7 Å，来自酶转换后生长的晶体。 C.E.尼科尔斯，A.R. 霍金斯和 D.K. 斯塔默萨。晶体学报 D 部分，第 60 卷，第 5 部分，第 971-973 页，2004 年 5 月。

2. Caspase 激活剂人粒酶 B 的晶体结构，一种对 Asp-P1 残基具有高度特异性的蛋白酶。 E. Estebanez-Perpina 等人。生物学。化学，卷。 381，第 1203-1214 页，2000 年 12 月。

Hampton蛋白结晶试剂盒Sodium citrate tribasic dihydrate Buffer/HR2-571/HR2-735

Products > Optimize Reagents > Optimize – Buffers > Sodium citrate tribasic dihydrate Buffer

Sodium citrate tribasic dihydrate Buffer

CAT NO

HR2-571

NAME

1.0 M Sodium citrate tribasic dihydrate

DESCRIPTION

100 mL

PRICE

$56.00

CAT NO

HR2-735

NAME

1.0 M Sodium citrate tribasic dihydrate pH 5.6

DESCRIPTION

100 mL

PRICE

$56.00

支持材料

HR2-571 Sodium citrate tribasic dihydrate SDS

HR2-735 Sodium citrate tribasic dihydrate pH 5.6 SDS

Titration Table

应用

Crystallization grade Sodium citrate tribasic dihydrate buffer for formulating screens or for optimization
结晶级柠檬酸钠三水合物缓冲液，用于配制筛选或优化

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

Sodium citrate tribasic dihydrate

Synonyms: Citric acid trisodium salt dihydrate or Trisodium citrate dihydrate
C₆H₅Na₃O₇• 2H₂O
HOC(COONa)(CH₂COONa)₂• 2H₂O
M_r 294.10
CAS Number [6132-04-3]
EC Number 200-675-3
Merck 14,8602
Beilstein Registry Number 6104939
MDL Number MFCD00150031
Purity ≥ 99.0%

Useful pH range of a Sodium citrate tribasic dihydrate buffer: 2.2 – 6.5

Titrate HR2-571 to the desired pH using HCl
HR2-571 Measured pH Range: 8.1 – 9.4 at 25°C
HR2-571 Measured Conductivity Range: 60.0 – 69.8 mS/cm at 25°C
HR2-571 Measured Refractive Index Range: 1.37345 – 1.37370 at 20°C

HR2-735 is titrated to pH 5.6 using HCl
HR2-735 Measured Conductivity Range: 67.4 – 78.5 mS/cm at 25°C
HR2-735 Measured Refractive Index Range: 1.37400 – 1.37502 at 20°C

别名：柠檬酸三钠盐二水合物或柠檬酸三钠二水合物
C6H5Na3O7 • 2H2O
HOC(COONa)(CH2COONa)2 • 2H2O
294.10 先生
CAS 编号 [6132-04-3]
欧盟编号 200-675-3
默克 14,8602
贝尔斯坦注册号 6104939
MDL 编号 MFCD00150031
纯度≥99.0%

柠檬酸钠三水合物缓冲液的有用 pH 范围：2.2 – 6.5

使用 HCl 将 HR2-571 滴定至所需的 pH 值
HR2-571 测量的 pH 范围：25°C 时 8.1 – 9.4
HR2-571 测量的电导率范围：25°C 时为 60.0 – 69.8 mS/cm
HR2-571 测量的折射率范围：20°C 时为 1.37345 – 1.37370

使用 HCl 将 HR2-735 滴定至 pH 5.6
HR2-735 测得的电导率范围：25°C 时为 67.4 – 78.5 mS/cm
HR2-735 测量的折射率范围：20°C 时为 1.37400 – 1.37502

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.005%
Cd ≤0.0005%
Cl ≤0.001%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.01%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
NH₄⁺ ≤0.001%
Ni ≤0.0005%
Pb ≤0.0005%
SO₄ ≤0.005%
Zn ≤0.0005%

Sodium citrate tribasic dihydrate

参考

1. Crystallization and preliminary X-ray diffraction studies on ADP-ribosylation factor 1. Greasley, S; Jhoti, H; Fensome, AC; Cockcroft, S; Thomas, GM; Bax, B. J Mol Biol 244, 651- 653, 1994.

1. ADP-核糖基化因子的结晶和初步 X 射线衍射研究 1. Greasley, S; 乔蒂，H；芬索姆，AC；考克罗夫特，S；托马斯，总经理； Bax, B. J Mol Biol 244, 651-653, 1994。

Hampton蛋白结晶试剂盒TMAO/HR2-857

Products > Optimize Reagents > Optimize – Cryoprotectants > TMAO

TMAO

CAT NO

HR2-857

NAME

4.0 M TMAO

DESCRIPTION

1 mL

PRICE

$26.00

支持材料

HR2-857 4.0 M TMAO SDS

应用

Water soluble cryoprotectant
水溶性冷冻保护剂

特征

A very versatile cryoprotectant for macromolecular crystals
Protein stabilizer
TMAO can be a viable alternative when conventional cryoprotectants fail
Low viscosity and low surface tension
Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
一种非常通用的大分子晶体冷冻保护剂
蛋白质稳定剂
当传统冷冻保护剂失效时，TMAO 可能是一种可行的替代方案
低粘度和低表面张力
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Trimethylamine N-oxide dihydrate (TMAO)

Formulated from TMAO dihydrate in Type 1 lab water (deionized) and sterile filtered (0.22 micron)

Synonyms: TMANO, TMAO
(CH₃)₃NO•2H₂O
C₃H₉NO•2H₂O
M_r 111.14
CAS Number [62637-93-8]
EC Number 214-675-6
Beilstein Registry Number 4(4)144
Merck 14,9711
RTECS YH2850000
Purity ≥ 98.0%
Measured pH range: 9.2 – 10.6 at 25°C
Measured Conductivity Range: 5.6 – 96.6 µS/cm at 25°C
Measured Refractive Index Range: 1.37660 – 1.37804 at 20°C

When using TMAO as a cryoprotectant, try soaking the crystals in 4.0 M TMAO for 10 seconds and then cryo cool directly in the cryo stream.

由 1 型实验室用水（去离子）和无菌过滤（0.22 微米）中的 TMAO 二水合物配制而成

同义词：TMANO、TMAO
(CH3)3NO•2H2O
C3H9NO•2H2O
111.14 先生
CAS 编号 [62637-93-8]
欧盟编号 214-675-6
贝尔斯坦注册号 4(4)144
默克 14,9711
RTECS YH2850000
纯度≥98.0%
测得的 pH 值范围：25°C 时 9.2 – 10.6
测得的电导率范围：25°C 时 5.6 – 96.6 µS/cm
测得的折射率范围：20°C 时为 1.37660 – 1.37804

当使用 TMAO 作为冷冻保护剂时，尝试将晶体浸泡在 4.0 M TMAO 中 10 秒，然后直接在冷冻流中冷冻冷却。

参考

1. Trimethylamine N-oxide as a versatile cryoprotective agent in macromolecular crystallography. Christoph Mueller-Dieckmann, Brice Kauffmann and Manfred S. Weiss. J. Appl. Cryst. (2011). 44, 433–436 doi:10.1107/S0021889811000045.

1. 三甲胺 N-氧化物作为大分子晶体学中的多功能冷冻保护剂。 Christoph Mueller-Dieckmann、Brice Kauffmann 和 Manfred S. Weiss。 J.应用。水晶。（2011）。 44, 433–436 doi:10.1107/S0021889811000045。

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725

Products > Optimize Reagents > Optimize – Buffers > Tris Buffer

Tris Buffer

CAT NO

HR2-589

NAME

1.0 M Tris

DESCRIPTION

100 mL

PRICE

$60.00

CAT NO

HR2-725

NAME

1.0 M Tris pH 8.5

DESCRIPTION

100 mL

PRICE

$60.00

应用

Crystallization grade Tris for formulating screens or for optimization
结晶级 Tris 用于配制筛选或优化

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Tris

Synonyms: Trizma base or 2-Amino-2-(hydroxymethyl)-1,3-propanediol or THAM or Tris base or Tris(hydroxymethyl)aminomethane or Trometamol
C₄H₁₁NO₃
NH₂C(CH₂OH)₃
M_r 121.14
CAS Number [77-86-1]
EC Number 201-064-4
Beilstein Registry Number 741883
Merck 14,9772
RTECS TY2900000
MDL Number MFCD00004679
PubChem Substance ID 24889854
Purity 99.8 – 100.1%
pKa = 8.1 at 25°C

Useful pH range of Tris: 7.0 – 9.0

Titrate HR2-589 to the desired pH using HCl
HR2-589 Measured pH Range: 10.9 – 11.1 at 25°C
HR2-589 Measured Conductivity Range: 193.3 – 236.7 µS/cm at 25°C
HR2-589 Measured Refractive Index: 1.34991 – 1.35058 at 20°C

HR2-725 is titrated to pH 8.5 using HCl
HR2-725 Measured Conductivity Range: 18.1 – 22.7 mS/cm at 25°C
HR2-725 Measured Refractive Index: 1.35310 – 1.35369 at 20°C

同义词： Trizma 碱或 2-Amino-2-(羟甲基)-1,3-丙二醇或 THAM 或 Tris 碱或 Tris(羟甲基)氨基甲烷或氨丁三醇
C4H11NO3
NH2C(CH2OH)3
121.14 先生
CAS 编号 [77-86-1]
欧盟编号 201-064-4
贝尔斯坦注册号 741883
默克 14,9772
RTECS TY2900000
MDL 编号 MFCD00004679
PubChem 物质 ID 24889854
纯度 99.8 – 100.1%
pKa = 8.1 在 25°C

Tris 的有用 pH 值范围：7.0 – 9.0

使用 HCl 将 HR2-589 滴定至所需的 pH 值
HR2-589 测得的 pH 范围：25°C 时为 10.9 – 11.1
HR2-589 测量的电导率范围：25°C 时为 193.3 – 236.7 µS/cm
HR2-589 实测折射率：20°C 时为 1.34991 – 1.35058

使用 HCl 将 HR2-725 滴定至 pH 8.5
HR2-725 测量的电导率范围：25°C 时为 18.1 – 22.7 mS/cm
HR2-725 实测折射率：20°C 时为 1.35310 – 1.35369

Insoluble Matter 0.005% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 5 ppm max
As ≤ 5 ppm max
Ca ≤ 5 ppm max
Cu ≤ 5 ppm max
Fe ≤ 5 ppm max
Pb ≤ 5 ppm max
Mg ≤ 5 ppm max

Trizma is a registered trademark of Sigma-Aldrich Co.

支持材料

HR2-589 Tris SDS

HR2-725 Tris pH 8.5 SDS

Titration Table

参考

1. Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase. Tian, Y; Beaman, TW; Roderick, SL; Proteins 28, 298- 300, 1997.

1. 铜绿假单胞菌氯霉素乙酰转移酶的纯化与结晶。田，Y；比曼，台湾；罗德里克，SL；蛋白质 28, 298-300, 1997。

Hampton蛋白结晶试剂盒NDSB-195/HR2-703

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-195

NDSB-195

CAT NO

HR2-703

NAME

NDSB-195

DESCRIPTION

5 grams

PRICE

$57.00

支持材料

HR2-703 NDSB-195 User Guide

HR2-703 NDSB-195 SDS

应用

Crystallization grade NDSB-195 for formulating screens or for optimization
结晶级 NDSB-195 用于配制筛选或优化

特征

Efficiently solubilizes proteins at non-denaturing concentrations
Useful in preventing protein aggregation
Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
Protein specific additive for solubility and stability
在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

The non detergent sulfobetaines are zwitterionic over a wide pH range, easily removed by dialysis and do not absorb significantly in the near UV range.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-195
Synonyms: Dimethylethylammonium propane sulfonate or Non-detergent sulfobetaine
C₇H₁₇NO₃S
M_r 195.30
Purity: ≥95% by TLC
Hygroscopic
Store at room temperature (+20 degrees Celsius)

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包含体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

非去污剂磺基甜菜碱在很宽的 pH 值范围内是两性离子的，很容易通过透析去除，并且在近紫外范围内不会显着吸收。

蛋白质样品中典型的有用 NDSB 浓度为 0.5-1.0 M。

NDSB-195
别名：二甲基乙基丙烷磺酸铵或非洗涤剂磺基甜菜碱
C7H17NO3S
195.30 先生
纯度：TLC ≥95%
吸湿性
常温（+20℃）保存

HR2-703

参考

1. Goldberg, M.E., et al. 1995. Folding & Design 1, 21.

2. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.

3. Vuillard, L., et al. 1995. Biochem. J. 305, 337.

4. Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

Hampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727

Products > Optimize Reagents > Optimize – Buffers > TRIS hydrochloride Buffer

TRIS hydrochloride Buffer

CAT NO

HR2-579

NAME

1.0 M TRIS hydrochloride

DESCRIPTION

100 mL

PRICE

$63.00

CAT NO

HR2-727

NAME

1.0 M TRIS hydrochloride pH 8.5

DESCRIPTION

100 mL

PRICE

$63.00

支持材料

HR2-579 TRIS hydrochloride SDS

HR2-727 TRIS hydrochloride pH 8.5 SDS

Titration Table

应用

Crystallization grade TRIS hydrochloride buffer for formulating screens or for optimization
用于配制筛选或优化的结晶级 TRIS 盐酸盐缓冲液

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述TRIS hydrochloride

Synonyms: TRIS HCl or Trizma hydrochloride or Tris(hydroxymethyl)aminomethane hydrochloride
NH₂C(CH₂OH)₃• HCl
C₄H₁₁NO₃• HCl
M_r 157.60
[1185-53-1]
EC Number 214-684-5
Beilstein Registry Number 3675235
MDL Number MFCD00012590
PubChem Substance ID 24900398
Purity ≥ 99.0%

Titrate HR2-579 to the desired pH using NaOH
HR2-579 Measured pH Range: 3.9 – 4.0 at 25°C
HR2-579 Measured Conductivity Range: 52.3 – 53.7 mS/cm at 25°C
HR2-579 Measured Refractive Index Range: 1.35840 – 1.35891 at 20°C

HR2-727 has been titrated to pH 8.5 using NaOH
HR2-727 Measured Conductivity Range: 59.8 – 61.7 mS at 25°C
HR2-727 Measured Refractive Index Range: 1.35938 – 1.36019 at 20°C

Use StockOptions Tris Hydrochloride kit (HR2-237) for a set of 21 Tris hydrochloride reagents titrated to pH 7.0 to 9.0 in 0.1 pH increments.

同义词：TRIS HCl 或 Trizma 盐酸盐或 Tris(羟甲基)氨基甲烷盐酸盐
NH2C(CH2OH)3 • HCl
C4H11NO3 • HCl
157.60 先生
[1185-53-1]
欧盟编号 214-684-5
贝尔斯坦注册号 3675235
MDL 编号 MFCD00012590
PubChem 物质 ID 24900398
纯度≥99.0%

使用 NaOH 将 HR2-579 滴定至所需的 pH 值
HR2-579 测量的 pH 范围：25°C 时 3.9 – 4.0
HR2-579 测量的电导率范围：25°C 时为 52.3 – 53.7 mS/cm
HR2-579 测量的折射率范围：20°C 时为 1.35840 – 1.35891

HR2-727 已使用 NaOH 滴定至 pH 8.5
HR2-727 测得的电导率范围：25°C 时为 59.8 – 61.7 mS
HR2-727 测量的折射率范围：20°C 时为 1.35938 – 1.36019

将 StockOptions Tris 盐酸试剂盒 (HR2-237) 用于一套 21 种 Tris 盐酸试剂，以 0.1 的 pH 增量滴定至 pH 7.0 至 9.0。

Al ≤0.0005%
As ≤0.0001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni ≤0.0005%
Pb ≤0.0005%
SO₄ ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%

TRIS hydrchoride

Hampton蛋白结晶试剂盒NDSB-201/HR2-701

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-201

NDSB-201

CAT NO

HR2-701

NAME

NDSB-201

DESCRIPTION

5 grams

PRICE

$43.00

支持材料

HR2-701 NDSB-201 User Guide

HR2-701 NDSB-201 SDS

应用

Crystallization grade NDSB-201 for formulating screens or for optimization
结晶级 NDSB-201 用于配制筛选或优化

特征

Efficiently solubilizes proteins at non-denaturing concentrations
Useful in preventing protein aggregation
Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
Protein specific additive for solubility and stability
在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

NDSB-201
Synonyms: 3-(1-Pyridino)-1-propane sulfonate
C₈H₁₁NO₃S
M_r 201.26
CAS Number [15471-17-7]
EC Number 239-491-3
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

参考

1. Vuillard, L., et al, J. Biochem., 305, 337 (1995).

2. Goldberg, M.E., et al, Folding and Design, 1, 21 (1996).

3. Vuillard et al. J.Cryst Growth (1996) vol 168 pp 150-154.

Hampton蛋白结晶试剂盒Hydrochloric acid/HR2-581

Products > Optimize Reagents > Optimize – Buffers > Hydrochloric acid

Hydrochloric acid

CAT NO

HR2-581

NAME

1.0 M Hydrochloric acid

DESCRIPTION

100 mL

PRICE

$38.00

支持材料

HR2-581 Hydrochloric acid SDS

应用

Crystallization grade Hydrochloric acid for formulating screens or for optimization
结晶级盐酸，用于筛选或优化

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Hydrochloric acid

Synonyms: None
HCl
M_r 36.46
CAS Number [7647-01-0]
EC Number 231-595-7
Beilstein Registry Number 1098214
Merck 14,4794
RTECS MW4025000
MDL Number MFCD00011324
PubChem Substance ID 329754537
UN Number (RIDADR) UN 1789 8/PG 2

Measured Conductivity Range: 320.4 – 378.2 mS/cm at 25°C

The 1.0 M Hydrochloric acid is formulated using pyrogen free (<0.03 Endotoxin (EU/ml)) water. The final 1.0 M Hydrochloric acid is not certified pyrogen free.

同义词：无
盐酸
36.46 先生
CAS 编号 [7647-01-0]
欧盟编号 231-595-7
贝尔斯坦注册号 1098214
默克 14,4794
RTECS MW4025000
MDL 编号 MFCD00011324
PubChem 物质 ID 329754537
联合国编号 (RIDADR) UN 1789 8/PG 2

测得的电导率范围：25°C 时 320.4 – 378.2 mS/cm

使用无热原 (<0.03 内毒素 (EU/ml)) 的水配制 1.0 M 盐酸。最后的 1.0 M 盐酸未经过认证无热原。

Ag ≤0.000001%
Al ≤0.000005%
As ≤0.0000005%
Ba ≤0.000002%
Bi ≤0.00001%
Br ≤0.005%
Ca ≤0.00005%
Cd ≤0.000001%
Co ≤0.000001%
Cr ≤0.000002%
Cu ≤0.000002%
Fe ≤0.00002%
Hg ≤0.00001%
K ≤0.00001%
Li ≤0.000001%
Mg ≤0.00001%
Mn ≤0.000001%
Mo ≤0.000002%
Na ≤0.00005%
NH₄⁺ ≤0.0001%
Ni ≤0.000002%
PO₄ ≤0.0005%
Pb ≤0.000002%
SO₄ ≤0.0001%
SO₃ ≤0.0001%
Sr ≤0.000001%
TI ≤0.000005%
Zn ≤0.000005%

Hydrochloric acid相关项目

BIS-TRIS Buffer
HEPES sodium Buffer
Imidazole Buffer
Sodium acetate trihydrate Buffer
Sodium cacodylate trihydrate Buffer
Sodium citrate tribasic dihydrate Buffer
Tris Buffer
BIS-TRIS 缓冲器
HEPES钠缓冲液
咪唑缓冲液
三水乙酸钠缓冲液
二甲胂酸钠三水合物缓冲液
柠檬酸钠二水合物缓冲液
Tris 缓冲区

Hampton蛋白结晶试剂盒NDSB-211/HR2-793

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-211

NDSB-211

CAT NO

HR2-793

NAME

NDSB-211

DESCRIPTION

5 grams

PRICE

$127.00

应用

Crystallization grade NDSB-211 for formulating screens or for optimization
结晶级 NDSB-211 用于配制筛选或优化

特征

Efficiently solubilizes proteins at non-denaturing concentrations
Useful in preventing protein aggregation
Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
Protein specific additive for solubility and stability
在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包涵体和细菌表达系统中发现的蛋白质。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-211
Synonyms: Dimethyl(2-hydroxyethyl)ammonium propane sulfonate or Non detergent sulfobetaine 211
C₇H₁₇NO₄S
M_r 211.30
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

支持材料HR2-793

HR2-793 NDSB-211 User Guide

HR2-793 NDSB-211 SDS

参考

1. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.

2. Vuillard, L., et al. 1995. Biochem. J. 305-, 337.

Hampton蛋白结晶试剂盒Sodium hydroxide/HR2-583

Products > Optimize Reagents > Optimize – Buffers > Sodium hydroxide

Sodium hydroxide

CAT NO

HR2-583

NAME

1.0 M Sodium hydroxide

DESCRIPTION

100 mL

PRICE

$38.00

支持材料

HR2-583 Sodium hydroxide SDS

应用

Crystallization grade Sodium hydroxide for formulating screens or for optimization
结晶级氢氧化钠，用于筛选或优化

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Sodium hydroxide

Synonym: Caustic soda
NaOH
HNaO
M_r 40.00
CAS [1310-73-2]
EC Number 215-185-5
Merck 14,8627
MDL Number MFCD00003548
PubChem Substance ID 24899773
UN Number UN 1823 8/PG 2
RTECS WB4900000
Purity ≥ 98.0%

Al ≤0.0005%
Ca ≤0.0005%
Cl ≤0.005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.02%
Mg ≤0.0005%
P ≤0.0005%
Pb ≤0.001%
SO₄ ≤0.05%
Zn ≤0.0005%

Sodium hydroxide

Hampton蛋白结晶试剂盒NDSB-221/HR2-791

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-221

NDSB-221

CAT NO

HR2-791

NAME

NDSB-221

DESCRIPTION

5 grams

PRICE

$58.00

支持材料

HR2-791 NDSB-221 User Guide

HR2-791 NDSB-221 SDS

应用

Crystallization grade NDSB-221 for formulating screens or for optimization
结晶级 NDSB-221 用于配制筛选或优化

特征

Efficiently solubilizes proteins at non-denaturing concentrations
Useful in preventing protein aggregation
Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
Protein specific additive for solubility and stability
在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包涵体和细菌表达系统中发现的蛋白质。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-221
Synonyms: Non-detergent sulfobetaine 221 or 3-(1-Methylpiperidinium)-1-propane Sulfonate
C₉H₁₉NO₃S
M_r 221.34
Purity: ≥99.0%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

HR2-791参考

1. Goldberg, M.E., et al. 1996. Folding & Design 1, 21.

2. Protein crystallography with non detergent sulfobetaines. L. Vuillard et al. J Cryst Growth (1996) 168 150-15.

3. Vuillard L, Rabilloud T, Goldberg ME, Eur J Biochem 1998 256(1):128-135. (protein folding).

Hampton蛋白结晶试剂盒Polyethylene glycol 200/HR2-601

Products > Optimize Reagents > Optimize – Polymers > Polyethylene glycol 200

Polyethylene glycol 200

CAT NO

HR2-601

NAME

100% Polyethylene glycol 200

DESCRIPTION

200 mL

PRICE

$42.00

支持材料

HR2-601 Polyethylene glycol 200 SDS

应用

Crystallization grade Polyethylene glycol 200 for formulating screens or for optimization
结晶级聚乙二醇 200，用于配制筛网或优化

描述Polyethylene glycol 200

Synonyms: PEG 200
H(OCH₂CH₂)_nOH
Average Formula weight (M_r) 200 (190-210)
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3600000
MDL Number MFCD00081839
PubChem Substance ID 24898381
Melting Point -65°C (lit.)
Density 1.1239 g/mL at 20°C
Vapor pressure <0.01 mm Hg at 20°C
Viscosity 4.3 cSt at 20°C
Refractive index N20/D 1.459 (lit.)
Aldehyde: None detected
Peroxide: None detected

Measured Conductivity Range: 2.5 – 9.7 µS/cm at 25°C
Measured Refractive Index Range: 1.46008 – 1.46124 at 20°C

同义词：PEG 200
H(OCH2CH2)nOH
平均配方重量 (Mr) 200 (190-210)
CAS 编号 [25322-68-3]
欧盟编号 500-038-2
默克 14,7568
RTECS TQ3600000
MDL 编号 MFCD00081839
PubChem 物质 ID 24898381
熔点 -65°C (lit.)
密度 1.1239 g/mL 在 20°C
蒸气压 <0.01 mm Hg at 20°C
粘度 4.3 cSt 在 20°C
折射率 N20/D 1.459 (lit.)
醛：未检测到
过氧化物：未检测到

测得的电导率范围：25°C 时为 2.5 – 9.7 µS/cm
测得的折射率范围：20°C 时为 1.46008 – 1.46124

Polyethylene glycol 200

参考

1. Crystallization and Preliminary Structural Studies of a Chorismate Mutase Catalytic Antibody Complexed With a Transition State Analog. Haynes, MR; Stura, EA; Hilvert, D; Wilson, IA. Proteins 18, 198- 200, 1994.

1. 与过渡态类似物复合的分支酸变位酶催化抗体的结晶和初步结构研究。海恩斯先生；斯图拉，EA；希尔弗特，D；威尔逊，IA。蛋白质 18、198-200、1994。

Hampton蛋白结晶试剂盒NDSB-256/HR2-705

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-256

NDSB-256

CAT NO

HR2-705

NAME

NDSB-256

DESCRIPTION

5 grams

PRICE

$57.00

支持材料

HR2-705 NDSB-256 User Guide

HR2-705 NDSB-256 SDS

应用

Crystallization grade NDSB-256 for formulating screens or for optimization
结晶级 NDSB-256 用于配制筛选或优化

特征

Efficiently solubilizes proteins at non-denaturing concentrations
Useful in preventing protein aggregation
Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
Protein specific additive for solubility and stability
在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包含体和细菌表达系统中发现的蛋白质。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-256
Synonyms: Dimethylbenzylammonium propane sulfonate
C₁₂H₁₉NO₃S
M_r 257.37
Purity: ≥99% by TLC
Hygroscopic
Store at room temperature (+20 degrees Celsius)

HR2-705

参考

1. Vuillard, L., et al, J. Biochem., 305, 337 (1995).

2. A nondetergent sulfobetaine prevents protein aggregation in microcalorimetric studies. Collins et al, Notes & Tips / Anal. Biochem. 352 (2006) 299-301.

Hampton蛋白结晶试剂盒18 mm Mounted CryoLoop™ – 20 micron/HR4-932/HR4-945/HR4-918

Products > Cryocrystallography > CryoLoops > 18 mm Mounted CryoLoop™ – 20 micron

18 mm Mounted CryoLoop™ – 20 micron

CAT NO

HR4-932

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.025 – 0.05 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-945

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.05 – 0.1 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-947

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.1 – 0.2 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-970

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.2 – 0.3 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-971

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.3 – 0.4 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-972

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.4 – 0.5 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-973

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.5 – 0.7 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-974

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.7 – 1.0 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-918

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

PRICE

$86.00

应用

Cryocrystallography
低温晶体学

特征

Ready to use 18 mm length MicroTube with CryoLoop
Synthetic CryoLoop on stainless steel MicroTube
Complete range of loop diameters from 0.025 – 1.0 mm
Compatible with CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap Spine HT
即用型 18 mm 长 MicroTube 和 CryoLoop
不锈钢 MicroTube 上的合成 CryoLoop
从 0.025 – 1.0 mm 的完整范围的环直径
与 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 和 CrystalCap Spine HT 兼容

描述

18 mm Mounted CryoLoops with 20 micron diameter nylon. Nylon loops are staked to hollow, stainless steel MicroTubes™ that are used to mount, freeze, and secure the crystal during cryocrystallographic procedures and X-ray data collection. The MicroTube on the 18 mm Mounted CryoLoops is 18 mm in length and ready to insert into CrystalCap Magnetic and CrystalCap HT. The 20 micron diameter nylon loops show minimal diffraction, are thin for fast cryo cooling of the crystal, strong, and aerodynamic.

For use with CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT systems. Not compatible with the CrystalCap Copper, CrystalCap Copper HT, or CrystalCap ALS HT.

18 毫米安装的 CryoLoops，带有 20 微米直径的尼龙。尼龙环固定在空心不锈钢 MicroTubes™ 上，用于在低温晶体学程序和 X 射线数据收集过程中安装、冷冻和固定晶体。安装在 18 mm CryoLoops 上的 MicroTube 长 18 mm，可插入 CrystalCap Magnetic 和 CrystalCap HT。直径为 20 微米的尼龙环显示出最小的衍射，很薄，可快速冷却晶体，坚固且符合空气动力学。

用于 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 和 CrystalCap SPINE HT 系统。与 CrystalCap Copper、CrystalCap Copper HT 或 CrystalCap ALS HT 不兼容。

Hampton蛋白结晶试剂盒18 mm Mounted CryoLoop™ – 20 micron/HR4-932/HR4-945/HR4-918

Products > Cryocrystallography > CryoLoops > 18 mm Mounted CryoLoop™ – 20 micron

18 mm Mounted CryoLoop™ – 20 micron

CAT NO

HR4-932

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.025 – 0.05 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-945

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.05 – 0.1 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-947

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.1 – 0.2 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-970

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.2 – 0.3 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-971

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.3 – 0.4 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-972

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.4 – 0.5 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-973

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.5 – 0.7 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-974

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

0.7 – 1.0 mm – 25 pack

PRICE

$74.00

CAT NO

HR4-918

NAME

18 mm Mounted CryoLoop – 20 micron

DESCRIPTION

Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

PRICE

$86.00

应用

Cryocrystallography
低温晶体学

特征

Ready to use 18 mm length MicroTube with CryoLoop
Synthetic CryoLoop on stainless steel MicroTube
Complete range of loop diameters from 0.025 – 1.0 mm
Compatible with CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap Spine HT
即用型 18 mm 长 MicroTube 和 CryoLoop
不锈钢 MicroTube 上的合成 CryoLoop
从 0.025 – 1.0 mm 的完整范围的环直径
与 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 和 CrystalCap Spine HT 兼容

描述

For use with CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT systems. Not compatible with the CrystalCap Copper, CrystalCap Copper HT, or CrystalCap ALS HT.

用于 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 和 CrystalCap SPINE HT 系统。与 CrystalCap Copper、CrystalCap Copper HT 或 CrystalCap ALS HT 不兼容。

Hampton蛋白结晶试剂盒NDSB-256-4T/HR2-794

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-256-4T

NDSB-256-4T

CAT NO

HR2-794

NAME

NDSB-256-4T

DESCRIPTION

5 grams

PRICE

$172.00

支持材料

HR2-794 NDSB-256-4T User Guide

HR2-794 NDSB-256-4T SDS

应用

Crystallization grade NDSB-256-4T for formulating screens or for optimization
结晶级 NDSB-256-4T 用于配制筛选或优化

特征

Efficiently solubilizes proteins at non-denaturing concentrations
Useful in preventing protein aggregation
Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
Protein specific additive for solubility and stability
NDSB 256 4T is a non-detergent sulfobetaine (NDSB) that prevents protein aggregation and facilitates protein folding by interacting with the early folding intermediates. Reported to improve the in vitro renaturation efficiency of reduced hen egg lysozyme (60% enzymatic activity at 600 mM NDSB-256-4T) and chemically unfolded tryptophan synthase ß2 subunit (100% enzymatic activity at 1 M NDSB-256-4T).
在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂
NDSB 256 4T 是一种非去污剂磺基甜菜碱 (NDSB)，可通过与早期折叠中间体相互作用来防止蛋白质聚集并促进蛋白质折叠。据报道可提高还原鸡蛋溶菌酶（在 600 mM NDSB-256-4T 下的酶活性为 60%）和化学展开的色氨酸合酶 ß2 亚基（在 1 M NDSB-256-4T 下的酶活性为 100%）的体外复性效率。

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包涵体和细菌表达系统中发现的蛋白质。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-256-4T
Synonym: 3-(4-tert-Butyl-1-pyridino)-1-propane sulfonate, SB256-4T, SN2564T
C₁₂H₁₉NO₃S
CAS Number [570412-84-9]
M_r 257.35
Purity = 98% by HPLC
Hygroscopic
Store at room temperature

参考NDSB-256-4T

1. Expert-Bezancon, N., et. al., Biophys Chem, 100, 469 (2003).

Hampton蛋白结晶试剂盒Mounted CryoLoop™ – 20 micron/HR4-963/HR4-959/HR4-957

Products > Cryocrystallography > CryoLoops > Mounted CryoLoop™ – 20 micron

Mounted CryoLoop™ – 20 micron

应用

Cryocrystallography
低温晶体学

特征

Synthetic CryoLoop on stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
Complete range of loop diameters from 0.025 – 1.0 mm
Compatible with all CrystalCap Systems
不锈钢 MicroTube 上的合成 CryoLoop
MicroTube 特别设计，带有 EasySnap 凹槽
从 0.025 – 1.0 mm 的完整范围的环直径
与所有 CrystalCap 系统兼容

描述

Note: If you are using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT, or CrystalCap SPINE HT, save time and money by using the ready to mount 18 mm Mounted CryoLoop.

Mounted CryoLoops with 20 micron diameter nylon. These nylon loops are bonded to hollow, stainless steel MicroTubes™ that are used to mount, freeze, and secure the crystal during cryocrystallographic procedures and X-ray data collection. The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and secure to the CrystalCap using epoxy or similar adhesive. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic..

Most data collection geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT, snap the MicroTube at the second notch from the bottom, creating an 18 mm MicroTube lenghth and secure to the CrystalCap using epoxy or similar adhesive. For a 22 mm over length using the CrystalCap Copper, CrystalCap Copper HT, CrystalCap ALS Copper HT snap the MicroTube at the fifth notch from the bottom (notch closest to the CryoLoop) and secure to the CrystalCap using epoxy or similar adhesive.

注意：如果您使用的是 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 或 CrystalCap SPINE HT，请使用可安装的 18 毫米已安装 CryoLoop 来节省时间和金钱。

安装有 20 微米直径尼龙的 CryoLoops。这些尼龙环与中空不锈钢 MicroTubes™ 粘合，用于在低温晶体学程序和 X 射线数据收集过程中安装、冷冻和固定晶体。 MicroTube 的长度为 24 毫米，专门设计了 10、12、14、18 和 21 毫米尺寸的 EasySnap™ 凹槽。要获得所需长度的 MicroTube，只需将 MicroTube 卡入所需长度并使用环氧树脂或类似粘合剂固定到 CrystalCap。这些尼龙环显示出最小的衍射，很薄，可以快速冷冻，坚固且符合空气动力学。

大多数数据收集几何结构更喜欢或要求帽底和梁之间有 22 毫米的长度。对于使用 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 和 CrystalCap SPINE HT 的 22 mm 总长度，将 MicroTube 从底部的第二个凹口处卡入，形成 18 mm 的 MicroTube 长度并使用环氧树脂或类似粘合剂固定到 CrystalCap。对于 22 mm 以上的长度，使用 CrystalCap Copper、CrystalCap Copper HT、CrystalCap ALS Copper HT 将 MicroTube 卡在从底部算起的第五个槽口（最靠近 CryoLoop 的槽口），并使用环氧树脂或类似粘合剂固定到 CrystalCap。

HR4-965 Mounted CryoLoop 0.7 – 1.0 mm

CAT NO

HR4-313

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.025 – 0.05 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-625

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.05 – 0.1 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-955

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.1 – 0.2 mm – 25 pack

PRICE

$84.00CAT NO

HR4-957

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.2 – 0.3 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-959

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.3 – 0.4 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-961

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.4 – 0.5 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-963

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.5 – 0.7 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-965

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.7 – 1.0 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-953

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

PRICE

$103.00

参考

1. FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: Structure, mechanism, and acyl carrier protein binding. Ryan M. Van Wagoner and Jon Clardy. Structure, 2006, Volume 14, Number 9, 1425-1435.

1. FeeM，一种来自未培养土壤微生物的 N-酰基氨基酸合酶：结构、机制和酰基载体蛋白结合。瑞安 M. 范瓦格纳和乔恩克拉迪。结构，2006 年，第 14 卷，第 9 期，1425-1435。

Hampton蛋白结晶试剂盒Mounted CryoLoop™ – 20 micron/HR4-963/HR4-959/HR4-957

Products > Cryocrystallography > CryoLoops > Mounted CryoLoop™ – 20 micron

Mounted CryoLoop™ – 20 micron

应用

Cryocrystallography
低温晶体学

特征

Synthetic CryoLoop on stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
Complete range of loop diameters from 0.025 – 1.0 mm
Compatible with all CrystalCap Systems
不锈钢 MicroTube 上的合成 CryoLoop
MicroTube 特别设计，带有 EasySnap 凹槽
从 0.025 – 1.0 mm 的完整范围的环直径
与所有 CrystalCap 系统兼容

描述

Note: If you are using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT, or CrystalCap SPINE HT, save time and money by using the ready to mount 18 mm Mounted CryoLoop.

注意：如果您使用的是 CrystalCap、CrystalCap ALS、CrystalCap ALS HT 或 CrystalCap SPINE HT，请使用可安装的 18 毫米已安装 CryoLoop 来节省时间和金钱。

HR4-965 Mounted CryoLoop 0.7 – 1.0 mm

CAT NO

HR4-313

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.025 – 0.05 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-625

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.05 – 0.1 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-955

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.1 – 0.2 mm – 25 pack

PRICE

$84.00CAT NO

HR4-957

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.2 – 0.3 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-959

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.3 – 0.4 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-961

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.4 – 0.5 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-963

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.5 – 0.7 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-965

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

0.7 – 1.0 mm – 25 pack

PRICE

$84.00

CAT NO

HR4-953

NAME

Mounted CryoLoop – 20 micron

DESCRIPTION

Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

PRICE

$103.00

参考

Hampton蛋白结晶试剂盒Ammonium acetate/HR2-799/HR2-565

Products > Optimize Reagents > Optimize – Salts > Ammonium acetate

Ammonium acetate

CAT NO

HR2-565

NAME

1.0 M Ammonium acetate

DESCRIPTION

100 mL

PRICE

$96.00

CAT NO

HR2-799

NAME

8.0 M Ammonium acetate

DESCRIPTION

200 mL

PRICE

$132.00

应用

Crystallization grade Ammonium acetate for formulating screens or for optimization
结晶级醋酸铵，用于筛选或优化

特征

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤溶液
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Ammonium acetate

Synonyms: None
C₂H₇NO₂
CH₃COONH₄
M_r 77.08
CAS Number [631-61-8]
EC Number 211-162-9
Beilstein Registry 4186741
Merck 14,495
RTECS AF3675000
MDL Number MFCD00013066
Purity ≥ 99.0%
UN Number UN 3077 9/PG 3

HR2-565 Measured pH range: 6.8 – 7.2 at 25°C
HR2-565 Measured Conductivity Range: 62.0 – 72.9 mS/cm at 25°C
HR2-565 Measured Refractive Index Range: 1.34344 – 1.34417 at 20°C

HR2-799 Measured pH range: 7.6 – 7.9 at 25°C
HR2-799 Measured Conductivity Range: 68.9 – 105.1 mS/cm at 25°C
HR2-799 Measured Refractive Index Range: 1.39674 – 1.40910 at 20°C

同义词：无
C2H7NO2
CH3COONH4
77.08 先生
CAS 编号 [631-61-8]
欧盟编号 211-162-9
贝尔斯坦注册 4186741
默克 14,495
RTECS AF3675000
MDL 编号 MFCD00013066
纯度≥99.0%
联合国编号 UN 3077 9/PG 3

HR2-565 测得的 pH 范围：25°C 时为 6.8 – 7.2
HR2-565 测得的电导率范围：25°C 时为 62.0 – 72.9 mS/cm
HR2-565 测量的折射率范围：20°C 时为 1.34344 – 1.34417

HR2-799 测得的 pH 值范围：25°C 时为 7.6 – 7.9
HR2-799 测得的电导率范围：25°C 时为 68.9 – 105.1 mS/cm
HR2-799 测量的折射率范围：20°C 时为 1.39674 – 1.40910

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0002%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni ≤0.0005%
NO₃ ≤0.001%
Pb ≤0.0005%
SO₄ ≤0.001%
Sr ≤0.0005%
Zn ≤0.0005%

Ammonium acetate

支持材料

HR2-565 Ammonium acetate 1.0 M solution SDS

HR2-799 Ammonium acetate 8.0 M solution SDS

Hampton蛋白结晶试剂盒Mounted CryoLoop™ – 10 micron/HR4-994/HR4-995/HR4-999/HR4-997

Products > Cryocrystallography > CryoLoops > Mounted CryoLoop™ – 10 micron

Mounted CryoLoop™ – 10 micron

CAT NO

HR4-994

NAME

Mounted CryoLoop – 10 micron

DESCRIPTION

0.025-0.05 mm – 25 pack

PRICE

$91.00

CAT NO

HR4-995

NAME

Mounted CryoLoop – 10 micron

DESCRIPTION

0.05 – 0.1 mm – 25 pack

PRICE

$91.00

CAT NO

HR4-997

NAME

Mounted CryoLoop – 10 micron

DESCRIPTION

0.1 – 0.2 mm – 25 pack

PRICE

$91.00

CAT NO

HR4-999

NAME

Mounted CryoLoop – 10 micron

DESCRIPTION

0.2 – 0.3 mm – 25 pack

PRICE

$91.00

应用

Cryocrystallography
低温晶体学

特征

Synthetic CryoLoop on stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
不锈钢 MicroTube 上的合成 CryoLoop
MicroTube 特别设计，带有 EasySnap 凹槽

描述

Mounted CryoLoops with 10 micron diameter nylon. The 10 micron Mounted CryoLoop shows less background diffraction than the 20 micron Mounted CryoLoop. Please note that the 10 micron Mounted CryoLoops are very flexible due to their small diameter. These nylon loops are bonded to hollow, stainless steel MicroTubes™ that are used to mount, freeze, and secure the crystal during cryocrystallographic procedures and X-ray data collection. The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and secure to the CrystalCap using epoxy or similar adhesive. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic.

安装有 10 微米直径尼龙的 CryoLoops。 10 微米安装的 CryoLoop 显示的背景衍射比 20 微米安装的 CryoLoop 少。请注意，10 微米安装的 CryoLoop 因其小直径而非常灵活。这些尼龙环与中空不锈钢 MicroTubes™ 粘合，用于在低温晶体学程序和 X 射线数据收集过程中安装、冷冻和固定晶体。 MicroTube 的长度为 24 毫米，专门设计了 10、12、14、18 和 21 毫米尺寸的 EasySnap™ 凹槽。要获得所需长度的 MicroTube，只需将 MicroTube 卡入所需长度并使用环氧树脂或类似粘合剂固定到 CrystalCap。这些尼龙环显示出最小的衍射，很薄，可以快速冷冻，坚固且符合空气动力学。

参考HR4-994 Mounted CryoLoop 0.025 – 0.05 mm

1. A novel approach to micro-sample X-ray powder diffraction using nylon loops. Reibenspies and Bhuvanesh. J. Appl. Cryst. (2003). 36, 1480-1481.

1. 一种使用尼龙环进行微量样品 X 射线粉末衍射的新方法。 Reibenspies 和 Bhuvanesh。 J.应用。水晶。（2003 年）。 36, 1480-1481。

Hampton蛋白结晶试剂盒Mounted CryoLoop™ – 10 micron/HR4-994/HR4-995/HR4-999/HR4-997

Products > Cryocrystallography > CryoLoops > Mounted CryoLoop™ – 10 micron

Mounted CryoLoop™ – 10 micron

CAT NO

HR4-994

NAME

Mounted CryoLoop – 10 micron

DESCRIPTION

0.025-0.05 mm – 25 pack

PRICE

$91.00

CAT NO

HR4-995

NAME

Mounted CryoLoop – 10 micron

DESCRIPTION

0.05 – 0.1 mm – 25 pack

PRICE

$91.00

CAT NO

HR4-997

NAME

Mounted CryoLoop – 10 micron

DESCRIPTION

0.1 – 0.2 mm – 25 pack

PRICE

$91.00

CAT NO

HR4-999

NAME

Mounted CryoLoop – 10 micron

DESCRIPTION

0.2 – 0.3 mm – 25 pack

PRICE

$91.00

应用

Cryocrystallography
低温晶体学

特征

Synthetic CryoLoop on stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
不锈钢 MicroTube 上的合成 CryoLoop
MicroTube 特别设计，带有 EasySnap 凹槽

描述

参考HR4-994 Mounted CryoLoop 0.025 – 0.05 mm

1. A novel approach to micro-sample X-ray powder diffraction using nylon loops. Reibenspies and Bhuvanesh. J. Appl. Cryst. (2003). 36, 1480-1481.

1. 一种使用尼龙环进行微量样品 X 射线粉末衍射的新方法。 Reibenspies 和 Bhuvanesh。 J.应用。水晶。（2003 年）。 36, 1480-1481。

Hampton蛋白结晶试剂盒Ammonium chloride/HR2-691

Products > Optimize Reagents > Optimize – Salts > Ammonium chloride

Ammonium chloride

CAT NO

HR2-691

NAME

5.0 M Ammonium chloride

DESCRIPTION

200 mL

PRICE

$89.00

支持材料

HR2-691 Ammonium chloride SDS

应用

Crystallization grade Ammonium chloride for formulating screens or for optimization
用于筛选或优化的结晶级氯化铵

特征

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤溶液
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Ammonium chloride

Synonyms: Salmiac
NH₄Cl
M_r 53.49
CAS Number [12125-02-9]
EC Number 235-186-4
Beilstein Registry Number 4371014
Merck 14,509
RTECS BP4550000
MDL Number MFCD00011420
PubChem Substance ID 24846273
UN Number UN 3077 9/PG 3
Purity ≥ 99.5%

Measured pH range of 5.0 M Ammonium chloride 4.1 – 4.4 at 25°C
Measured Conductivity Range: 539.2 – 588.0 mS/cm at 25°C
Measured Refractive Index Range: 1.38048 – 1.38078 at 20°C

同义词： Salmiac
氯化铵
53.49 先生
CAS 编号 [12125-02-9]
欧盟编号 235-186-4
贝尔斯坦注册号 4371014
默克 14,509
RTECS BP4550000
MDL 编号 MFCD00011420
PubChem 物质 ID 24846273
联合国编号 UN 3077 9/PG 3
纯度≥99.5%

在 25°C 时测得的 5.0 M 氯化铵 4.1 – 4.4 的 pH 值范围
测得的电导率范围：25°C 时 539.2 – 588.0 mS/cm
测得的折射率范围：20°C 时为 1.38048 – 1.38078

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0002%
Fe ≤0.0002%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni ≤0.0001%
NO₃ ≤0.0005%
Pb ≤0.0001%
PO₄ ≤0.0002%
SO₄ ≤0.002%
Sr ≤0.0005%
Zn ≤0.0002%

参考Ammonium chloride

1. Structural studies on transfer RNA: preliminary crystallographic analysis. Labanauskas, M; Conners, PG; Young, JD; Bock, RM; Anderegg, JW; Beeman, WW. Science 166, 1530- 1532, 1969.

2. Crystal structure of the kringle 2 domain of tissue plasminogen activator at 2.4-A resolution. de Vos, AM; Ultsch, MH; Kelley, RF; Padmanabhan, K; Tulinsky, A; Westbrook, ML; Kossiakoff, AA. Biochemistry 31, 270- 279, 1992.

1. 转移 RNA 的结构研究：初步晶体学分析。拉巴瑙斯卡斯，M；康纳斯，PG；杨，法学博士；博克，RM；安德雷格，JW；比曼，WW。科学 166、1530-1532、1969。

2. 2.4-A 分辨率下组织型纤溶酶原激活剂的 kringle 2 结构域的晶体结构。德沃斯，上午；乌尔奇，MH；凯利，射频；帕德马纳班，K；图林斯基，A；威斯布鲁克，ML；科西亚科夫，AA。生物化学 31, 270-279, 1992。

Hampton蛋白结晶试剂盒Adjustable Mounted CryoLoop™/HR5-900/HR4-336/HR8-104

Products > Cryocrystallography > CryoLoops > Adjustable Mounted CryoLoop™

Adjustable Mounted CryoLoop™

应用

Cryocrystallography
低温晶体学

特征

Adjustable loop orientation
Synthetic CryoLoop on adjustable stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
20 micron diameter nylon CryoLoop
可调环方向
可调节不锈钢 MicroTube 上的合成 CryoLoop
MicroTube 特别设计，带有 EasySnap 凹槽
20 微米直径尼龙 CryoLoop

描述

Similar to the Mounted CryoLoop™ in design and function, the Adjustable Mounted CryoLoop allows one to manipulate and orient the CryoLoop to multiple, unique positions previously unattainable with typical cryo setups. The Adjustable Mounted CryoLoop is a 20 micron diameter synthetic loop mounted inside a malleable stainless steel sleeve, which in turn, is mounted inside the EasySnap MicroTube™.

Using forceps or a probe one can manipulate the angle and orientation of the CryoLoop by bending and adjusting the angle of the malleable stainless steel insert which holds the CryoLoop. The Adjustable Mounted CryoLoop may be positioned several times. Note: Repeated manipulation will fatigue the sleeve which can lead to failure of the sleeve. The Adjustable Mounted CryoLoop is designed to work with the CrystalCap System components.

The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and stake it to the CrystalCap™ Magnetic or CrystalCap™ Copper Magnetic. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic.

在设计和功能上与 Mounted CryoLoop™ 类似，可调节的 Mounted CryoLoop 允许人们操纵 CryoLoop 并将其定位到多个独特的位置，这些位置以前是典型的低温设置无法达到的。可调节安装的 CryoLoop 是一个直径 20 微米的合成环，安装在可延展的不锈钢套管内，而不锈钢套管又安装在 EasySnap MicroTube™ 内。

使用镊子或探针可以通过弯曲和调整固定 CryoLoop 的可延展不锈钢插件的角度来控制 CryoLoop 的角度和方向。可调节安装的 CryoLoop 可以多次定位。注意：重复操作会使套筒疲劳，从而导致套筒失效。可调节安装的 CryoLoop 设计用于与 CrystalCap 系统组件一起使用。

MicroTube 的长度为 24 毫米，专门设计了 10、12、14、18 和 21 毫米尺寸的 EasySnap™ 凹槽。要获得所需长度的 MicroTube，只需将 MicroTube 卡入所需长度并将其固定在 CrystalCap™ Magnetic 或 CrystalCap™ Copper Magnetic 上。这些尼龙环显示出最小的衍射，很薄，可以快速冷冻，坚固且符合空气动力学。

HR8-110 Adjustable Mounted CryoLoop 0.7 – 1.0 mm

CAT NO

HR4-336

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.025 – 0.05 mm – 25 pack

PRICE

$107.00

CAT NO

HR4-338

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.05 – 0.1 mm – 25 pack

PRICE

$107.00

CAT NO

HR8-102

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.1 – 0.2 mm – 25 pack

PRICE

$107.00

CAT NO

HR8-072

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.2 – 0.3 mm – 25 pack

PRICE

$107.00

CAT NO

HR8-104

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.3 – 0.4 mm – 25 pack

PRICE

$107.00

CAT NO

HR8-106

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.4 – 0.5 mm – 25 pack

PRICE

$107.00

CAT NO

HR8-108

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.5 – 0.7 mm – 25 pack

PRICE

$107.00

CAT NO

HR8-110

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.7 – 1.0 mm – 25 pack

PRICE

$107.00

CAT NO

HR5-900

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

PRICE

$128.00

Hampton蛋白结晶试剂盒Adjustable Mounted CryoLoop™/HR5-900/HR4-336/HR8-104

Products > Cryocrystallography > CryoLoops > Adjustable Mounted CryoLoop™

Adjustable Mounted CryoLoop™

应用

Cryocrystallography
低温晶体学

特征

Adjustable loop orientation
Synthetic CryoLoop on adjustable stainless steel MicroTube
MicroTube specially engineered with EasySnap notches
20 micron diameter nylon CryoLoop
可调环方向
可调节不锈钢 MicroTube 上的合成 CryoLoop
MicroTube 特别设计，带有 EasySnap 凹槽
20 微米直径尼龙 CryoLoop

描述

HR8-110 Adjustable Mounted CryoLoop 0.7 – 1.0 mm

CAT NO

HR4-336

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.025 – 0.05 mm – 25 pack

PRICE

$107.00

CAT NO

HR4-338

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.05 – 0.1 mm – 25 pack

PRICE

$107.00

CAT NO

HR8-102

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.1 – 0.2 mm – 25 pack

PRICE

$107.00

CAT NO

HR8-072

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.2 – 0.3 mm – 25 pack

PRICE

$107.00

CAT NO

HR8-104

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.3 – 0.4 mm – 25 pack

PRICE

$107.00

CAT NO

HR8-106

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.4 – 0.5 mm – 25 pack

PRICE

$107.00

CAT NO

HR8-108

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.5 – 0.7 mm – 25 pack

PRICE

$107.00

CAT NO

HR8-110

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

0.7 – 1.0 mm – 25 pack

PRICE

$107.00

CAT NO

HR5-900

NAME

Adjustable Mounted CryoLoop

DESCRIPTION

Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm)

PRICE

$128.00

Hampton蛋白结晶试剂盒Ammonium citrate dibasic/HR2-685

Products > Optimize Reagents > Optimize – Salts > Ammonium citrate dibasic

Ammonium citrate dibasic

CAT NO

HR2-685

NAME

2.5 M Ammonium citrate dibasic

DESCRIPTION

200 mL

PRICE

$128.00

应用

Crystallization grade Ammonium citrate dibasic for formulating screens or for optimization
结晶级柠檬酸氢铵，用于配制筛选或优化

特征

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤溶液
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Ammonium citrate dibasic

Synonyms: Citric acid ammonium salt or Diammonium hydrogen citrate or Di-Ammnonium citrate or Ammonium hydrogencitrate

别名：柠檬酸铵盐或柠檬酸氢二铵或柠檬酸二铵或柠檬酸氢铵
C₆H₁₄N₂O₇
(NH₄)₂C₆H₆O₇
HOC(CO₂H)(CH₂CO₂NH₄)₂
M_r 226.19
CAS Number 3012-65-5
EC Number 221-146-3
Merck 14,512
Beilstein Registry Number 4925760
RTECS GE7545000
MDL Number MFCD00013068
PubChem Substance ID 24846459
Purity ≥ 99.0%

Measured pH range: 4.7 – 4.8 at 25°C
Measured Conductivity Range: 76.8 – 83.1 mS/cm at 25°C
Measured Refractive Index Range: 1.41993 – 1.42027 at 20°C

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni ≤0.0005%
Pb ≤0.0005%
PO₄ ≤0.0005%
SO₄ ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%

参考Ammonium citrate dibasic

HR2-685 Ammonium citrate dibasic SDS

参考

1. Structure of serum response factor core bound to DNA. Pellegrini, L; Tan, S; Richmond, TJ. Nature 376, 490- 498, 1995.

2. Crystal structure of the ligand-binding domain of the human nuclear receptor RXR-alpha. Bourguet, W; Ruff, M; Chambon, P; Gronemeyer, H; Moras, D. Nature 375, 377- 382, 1995.

1. 与 DNA 结合的血清反应因子核心结构。佩莱格里尼，L；谭，S；里士满，TJ。自然 376, 490-498, 1995。

2.人核受体RXR-α的配体结合结构域的晶体结构。布尔格，W；拉夫，M；尚邦，P；格罗内迈尔，H； Moras, D. Nature 375, 377-382, 1995。

Hampton蛋白结晶试剂盒Ammonium citrate tribasic/HR2-759/HR2-865

Products > Optimize Reagents > Optimize – Salts > Ammonium citrate tribasic

Ammonium citrate tribasic

CAT NO

HR2-759

NAME

2.5 M Ammonium citrate tribasic pH 7.0

DESCRIPTION

200 mL

PRICE

$128.00

CAT NO

HR2-865

NAME

2.5 M Ammonium citrate tribasic

DESCRIPTION

100 mL

PRICE

$87.00

支持材料

HR2-759 Ammonium citrate tribasic pH 7.0 SDS

HR2-865 Ammonium citrate tribasic SDS

应用

Crystallization grade Ammonium citrate tribasic for formulating screens or for optimization
用于配制筛选或优化的结晶级柠檬酸三铵

特征

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤溶液
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Ammonium citrate tribasic

Synonyms: Citric acid triammonium salt or Triammonium citrate
HOC(COONH₄)(CH₂COONH₄)₂
C₆H₁₇N₃O₇
M_r 243.22
CAS Number [3458-72-8]
EC Number 222-394-5
Beilstein Registry Number 3779194
MDL Number MFCD00036406
PubChem Substance ID 24890533
Purity ≥ 97.0%

Appearance (Solution): Clear with a very light yellow to brown tint color

HR2-759 titrated to pH 7.0 using HCl (used in SaltRx, Index, PEG/Ion, PegRx, and PEG/Ion 400)
Measured Conductivity Range: 95.4 – 97.9 mS/cm at 25°C
Measured Refractive Index Range: 1.43139 – 1.43241 at 20°C

HR2-865 is not titrated to a specific pH (used in GRAS Screen 1, 2, 3, 4, 7, and PEG/Ion 400)
Measured pH Range: 7.7 at 25°C
Measured Conductivity Range: 95.9 – 96.2 mS/cm at 25°C
Measured Refractive Index Range: 1.43204 – 1.43211 at 20°C

别名：柠檬酸三铵盐或柠檬酸三铵
HOC(COONH4)(CH2COONH4)2
C6H17N3O7
243.22 先生
CAS 编号 [3458-72-8]
欧盟编号 222-394-5
贝尔斯坦注册号 3779194
MDL 编号 MFCD00036406
PubChem 物质 ID 24890533
纯度≥97.0%

外观（溶液）：透明，带有非常浅的黄色至棕色色调

HR2-759 使用 HCl 滴定至 pH 7.0（用于 SaltRx、Index、PEG/Ion、PegRx 和 PEG/Ion 400）
测得的电导率范围：25°C 时 95.4 – 97.9 mS/cm
测得的折射率范围：20°C 时为 1.43139 – 1.43241

HR2-865 未滴定至特定 pH 值（用于 GRAS Screen 1、2、3、4、7 和 PEG/Ion 400）
测得的 pH 范围：25°C 时为 7.7
测得的电导率范围：25°C 时 95.9 – 96.2 mS/cm
测得的折射率范围：20°C 时为 1.43204 – 1.43211

Ammonium citrate tribasic

Hampton蛋白结晶试剂盒Epoxy/HR4-347

Products > Cryocrystallography > CryoLoops > Epoxy

Epoxy

CAT NO

HR4-347

NAME

Double/Bubble Epoxy

DESCRIPTION

5 pack

PRICE

$15.00

支持材料

HR4-347 Double/Bubble Epoxy SDS

应用

Sealing capillary tubes
Securing MicroTubes into CrystalCaps
Securing CryoLoops into MicroTubes
密封毛细管
将微管固定在 CrystalCaps 中
将 CryoLoop 固定到 MicroTube 中

特征

Fast setting
Excellent cohesive strength
快速设置
优异的内聚强度

描述

Double/Bubble® Epoxy HR4-347 extra fast setting with a work time of 3 to 5 minutes. The preferred epoxy for cryocrystallography. Sold as a pack of 5. Hardman Double/Bubble Fast-Setting Epoxy is an extra fast setting, two part, room temperature curing epoxy adhesive. It has a convenient mix ratio, excellent cohesive strength and bonds to most substrates including metal, wood, concrete, ceramics and many plastics. The Double/Bubble package is a handy, dual-pouch, one-shot, job-size packaging concept. Fills gaps, will not shrink. Contains no solvents. Two part epoxy in a single, dual-pouch 3.5 gram packet.

To use:
1. Fold Double/Bubble packet
2. Cut or tear to open end
3. Squeeze out adhesive
4. Mix parts A and B together with supplied spatula

Supplied as 5 packets with 5 spatulas.

Double/Bubble® Epoxy HR4-347 超快速设置，工作时间为 3 至 5 分钟。低温晶体学的首选环氧树脂。一包 5 件出售。 Hardman Double/Bubble Fast-Setting Epoxy 是一种超快速固化的两部分室温固化环氧树脂粘合剂。它具有方便的混合比、出色的内聚强度以及与大多数基材的粘合性，包括金属、木材、混凝土、陶瓷和许多塑料。 Double/Bubble 包装是一种方便、双袋、一次性、工作大小的包装概念。填补空白，不会缩小。不含溶剂。单双袋 3.5 克小包中的两部分环氧树脂。

要使用：
1.折叠双/气泡包
2. 切开或撕开端
3.挤出粘合剂
4. 用随附的抹刀将 A 部分和 B 部分混合在一起

以 5 包 5 把抹刀的形式提供。

Color/Appearance: Amber
Product Name: Hardman DOUBLE/BUBBLE Extra-Fast Set Epoxy Red Package 3.5 g Packet 1 part or 2: 2 part
Base Chemistry: Part A: Epoxy; Part B: Polymer
Handling strength: 15 to 30 minutes at 25°C
Cure Time: 24h at 25°C
Durometer/Hardness: 80 D at 25°C
Flash Point: Part A: 190.5°C; Part B: 132.2°C
Mix Ratio: 1:1
Peel Strength: 1.8 pli at 25°C
Service Temperature: Up to 82°C
Shear Strength Psi: 3,000 at 25°C
Specific Gravity: Mixed: 1.16 at 25°C
Viscosity Cp: Mixed: 40,000 at 25°C
Work Life/Pot Life: 3 minutes

Hampton蛋白结晶试剂盒Loctite® Easy-Squeeze Super Glue Gel/HR4-316

Products > Cryocrystallography > CryoLoops > Loctite® Easy-Squeeze Super Glue Gel

Loctite® Easy-Squeeze Super Glue Gel

CAT NO

HR4-316

NAME

Super Glue

DESCRIPTION

4 g tube

PRICE

$7.00

支持材料

HR4-316 Super Glue SDS

应用

Staking Glass Fibers to Brass Specimen Pins.
Staking MicroTube to CrystalCap
将玻璃纤维钉在黄铜试样销上。
将 MicroTube 质押到 CrystalCap

特征

Squeeze grips allow for precise application, keeping fingers glue free
Locking cap collar prevents leaks and drying out
Bonds more surfaces than ordinary glues, including: metal, CryoLoops, wood, paper, cardboard, vinyl, leather, rubber, cork, ceramic, china, textiles, brick and fingers.
挤压手柄允许精确应用，保持手指无胶水
锁定帽领防止泄漏和干燥
比普通胶水可以粘合更多的表面，包括：金属、CryoLoops、木材、纸张、纸板、乙烯基、皮革、橡胶、软木、陶瓷、瓷器、纺织品、砖块和手指。

描述

Loctite^® Easy-Squeeze Super Glue Gel for securing CryoLoops into MicroTubes and staking MicroTubes to CrystalCaps™. Net weight 4 grams / 0.14 ounces.

Loctite® Easy-Squeeze Super Glue Gel 用于将 CryoLoops 固定到 MicroTubes 中并将 MicroTubes 固定到 CrystalCaps™ 上。净重 4 克/0.14 盎司。

Hampton蛋白结晶试剂盒Ammonium fluoride/HR2-689

Products > Optimize Reagents > Optimize – Salts > Ammonium fluoride

Ammonium fluoride

CAT NO

HR2-689

NAME

10.0 M Ammonium fluoride

DESCRIPTION

200 mL

PRICE

$98.00

支持材料

HR2-689 Ammonium fluoride SDS

应用

Crystallization grade Ammonium fluoride for formulating screens or for optimization
用于配制筛选或优化的结晶级氟化铵

特征

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤溶液
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Ammonium fluoride

Synonyms: None
NH₄F
M_r 37.04
CAS Number [12125-01-8]
EC Number 235-185-9
Merck 14,522
RTECS BQ6300000
MDL Number MFCD00011423
PubChem Substance ID 24858156
UN Number 2505 6.1/PG 3
Purity ≥ 98.0%

Measured Conductivity Range: 350.8 – 472.6 mS/cm at 25°C
Measured Refractive Index Range: 1.34965 – 1.35993 at 20°C

同义词：无
NH4F
37.04 先生
CAS 编号 [12125-01-8]
欧盟编号 235-185-9
默克 14,522
RTECS BQ6300000
MDL 编号 MFCD00011423
PubChem 物质 ID 24858156
联合国编号 2505 6.1/PG 3
纯度≥98.0%

测得的电导率范围：25°C 时 350.8 – 472.6 mS/cm
测得的折射率范围：20°C 时为 1.34965 – 1.35993

Total Impurities: Passes filter test
Insoluble matter: ≤0.005%
Cl ≤0.001%
Fe 5ppm
Pb 5ppm
SO₄ ≤0.005%

Ammonium fluoride

参考

1. Crystallization and preliminary X-ray diffraction studies of -phosphoglucomutase from Lactococcus lactus. S. D. Lahiri, G. Zhang, P. Radstrom, D. Dunaway-Mariano and K. N. Allen. Acta Cryst. (2002). D58, 324-326.

1. 来自乳球菌的β-磷酸葡萄糖变位酶的结晶和初步 X 射线衍射研究。 S. D. Lahiri、G. Zhang、P. Radstrom、D. Dunaway-Mariano 和 K. N. Allen。晶体学报。（2002 年）。 D58，324-326。

Hampton蛋白结晶试剂盒Crystal Crusher/HR4-216

Products > Tools & Seeding > Crystal Crusher > Crystal Crusher

Crystal Crusher

CAT NO

HR4-216

NAME

Crystal Crusher

DESCRIPTION

5 pack

PRICE

$25.00

支持材料

HR4-216 Crystal Crusher User Guide

应用

Crush macro crystals to create micro crystals
粉碎宏观晶体以产生微晶体

特征

Solid, borosilicate glass tool and handle
Smooth, hemispherical tip, will not damage crystallization plate
Prepare micro crystals for seed stock for Microseed Matrix Screening(MMS)
坚固的硼硅酸盐玻璃工具和手柄
光滑，半球形尖端，不会损坏结晶板
为微种子基质筛选 (MMS) 的种子储备制备微晶体

描述

The Crystal Crusher is designed to crush macro crystals into micro crystals for seeding and other applications. The hemispherical end of the tool fits round, concave bottomed sitting drop crystallization plates for efficient crystal crushing. The Crystal Crusher is molded from solid, borosilicate glass. One end of the tool features a larger diameter, cylindrical handle, while the opposite end features a smooth, hemispherical end for crushing crystals without damaging crystallization plates.

Overall length: ~ 43 mm
Diameter of handle: ~ 3 mm
Diameter of hemispherical end: ~ 1 mm

Five Crystal Crushers per pack.

Crystal Crusher 旨在将大晶体粉碎成微晶体，用于播种和其他应用。该工具的半球形端适合圆形、凹底坐滴结晶板，可有效破碎晶体。 Crystal Crusher 由坚固的硼硅酸盐玻璃模制而成。该工具的一端具有较大直径的圆柱形手柄，而另一端具有光滑的半球形端，用于破碎晶体而不会损坏结晶板。

总长度：~ 43 毫米
手柄直径：~ 3 mm
半球端直径：~ 1 mm

每包五个水晶破碎机。

参考

1. Microseed matrix screening for optimization in protein crystallization: what have we learned? D’Arcy A, Bergfors T, Cowan-Jacob SW, Marsh M. Acta Crystallogr F Struct Biol Commun. 2014 Sep;70 (Pt 9):1117-26. doi: 10.1107/S2053230X14015507. Epub 2014 Aug 29.

1. 用于优化蛋白质结晶的微种子基质筛选：我们学到了什么？ D’Arcy A、Bergfors T、Cowan-Jacob SW、Marsh M. Acta Crystallogr F Struct Biol Commun。 2014 年 9 月；70（第 9 部分）：1117-26。 doi: 10.1107/S2053230X14015507。电子版 2014 年 8 月 29 日。

Hampton蛋白结晶试剂盒Ammonium formate/HR2-659

Products > Optimize Reagents > Optimize – Salts > Ammonium formate

Ammonium formate

CAT NO

HR2-659

NAME

10.0 M Ammonium formate

DESCRIPTION

200 mL

PRICE

$192.00

支持材料

HR2-659 Ammonium formate SDS

应用

Crystallization grade Ammonium formate for formulating screens or for optimization
结晶级甲酸铵，用于筛选或优化

特征

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤溶液
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Ammonium formate

Synonyms: Formic acid ammonium salt
HCOONH₄
CH₅NO₂
M_r 63.06
CAS 540-69-2
EC Number 208-753-9
Beilstein Registry Number 3625095
Merck 14,523
RTECS BQ6650000
MDL Number MFCD00013103
PubChem Substance ID 24846279
Purity ≥ 99.0%

Measured pH Range: 7.0 – 7.3 at 25°C
Measured Conductivity Range: 515.6 – 725.2 mS/cm at 25°C
Measured Refractive Index Range: 1.38437 – 1.40423 at 20°C

别名：甲酸铵盐
HCOONH4
CH5NO2
63.06 先生
CAS 540-69-2
欧盟编号 208-753-9
贝尔斯坦注册号 3625095
默克 14,523
RTECS BQ6650000
MDL 编号 MFCD00013103
PubChem 物质 ID 24846279
纯度≥99.0%

测得的 pH 值范围：25°C 时 7.0 – 7.3
测得的电导率范围：25°C 时 515.6 – 725.2 mS/cm
测得的折射率范围：20°C 时为 1.38437 – 1.40423

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni ≤0.0005%
Pb ≤0.0005%
SO₄ ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%

参考Ammonium formate

1. Picornaviruses of two different genera have similar structures. Luo, M; Arnold, E; Erickson, JW; Rossmann, MG; Boege, U; Scraba, DG. J Mol Biol 180, 703- 714, 1984.

2. The crystal structure of the Venezuelan equine encephalitis alphavirus nsP2 protease. Andrew T. Russo, Mark A. White, and Stanley J. Watowich. Structure 14, 1449-1458, September 2006.

1. 两个不同属的小核糖核酸病毒具有相似的结构。罗，男；阿诺德，E；埃里克森，JW；罗斯曼，MG；博格，U；斯克拉巴，DG。分子生物学杂志 180, 703-714, 1984。

2.委内瑞拉马脑炎甲病毒nsP2蛋白酶的晶体结构。 Andrew T. Russo、Mark A. White 和 Stanley J. Watowich。结构 14，1449-1458，2006 年 9 月。