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• dsSafe Gel Staining Solution 10,000×

dsSafe Gel Staining Solution 10,000×

The dsSafe dye is a fluorescent dye for nucleic acid staining in agarose and polyacrylamide gels. dsSafe is the best and safer alternative to ethidium bromide (EtBr); it was shown to be less toxic, non-mutagenic, and non-carcinogenic.

dsSafe allows nucleic acid visualization with sensitivity similar to that of ethidium bromide. Dye excitation can be performed with both conventional UV transilluminators and blue light transilluminators, with the latter ones being safer for the eyes and nucleic acid sample. When blue light is used for dsSafe excitation, background fluorescence is lower than in the case of ethidium bromide excitation with UV light. Excised DNA fragments for subsequent cloning are not exposed to damaging UV radiation, thus increasing cloning efficacy and reducing the frequency of adverse mutations caused by UV.

The spectrum of the DNA-dye complex has two excitation maxima [in the UV (280 nm) and blue ranges (502 nm)] and one emission maximum in the green range (~530 nm). Available as 10,000× concentrate in DMSO. To stain the gel, add the dye to melted agarose solution* or stain the gel after gel electrophoresis in TAE/TBE buffer for 10–20 minutes.

dsSafe 染料是一种荧光染料，用于琼脂糖和聚丙烯酰胺凝胶中的核酸染色。 dsSafe 是溴化乙锭 (EtBr) 的最佳和更安全的替代品；它被证明是毒性较小、非致突变性和非致癌性的。

dsSafe 允许核酸可视化，其灵敏度与溴化乙锭相似。染料激发可以使用传统的紫外透射仪和蓝光透射仪进行，后者对眼睛和核酸样品更安全。当蓝光用于 dsSafe 激发时，背景荧光低于用紫外光激发溴化乙锭的情况。用于后续克隆的切除 DNA 片段不会暴露在有害的紫外线辐射下，从而提高克隆效率并降低紫外线引起的不良突变频率。

DNA-染料复合物的光谱有两个激发最大值 [在 UV (280 nm) 和蓝色范围 (502 nm)] 和一个发射最大值在绿色范围 (~530 nm)。以 DMSO 中的 10,000 倍浓缩物形式提供。要对凝胶进行染色，请将染料添加到融化的琼脂糖溶液中*，或在凝胶电泳后在 TAE/TBE 缓冲液中染色 10-20 分钟。

Key dye advantages主要染料优势:

• Non-mutagenic, non-carcinogenic, far less toxic dye for DNA and RNA staining用于 DNA 和 RNA 染色的非诱变、非致癌、毒性低得多的染料
• High sensitivity identical to that of EtBr与 EtBr 相同的高灵敏度
• Excitation both with UV and blue light紫外光和蓝光激发
• Easy to use: the dye can be added to melted agarose solution or the gel can be stained within 10 to 20 minutes after gel electrophoresis completion
• 易于使用：染料可添加到融化的琼脂糖溶液中或凝胶电泳完成后 10 至 20 分钟内染色
• Can be used for all routine laboratory procedures requiring nucleic acid visualization可用于所有需要核酸可视化的常规实验室程序
• Does not require special storage or disposal conditions不需要特殊的存储或处置条件

For nucleic acid gel staining, we also offer dsGreen Gel Staining Solution with higher sensitivity to DNA . However, in contrast with dsSafe, gel staining with dsGreen Gel Staining Solution is recommended only after the completion of gel electrophoresis.

对于核酸凝胶染色，我们还提供对 DNA 具有更高灵敏度的 dsGreen 凝胶染色溶液。 然而，与 dsSafe 相比，建议仅在完成凝胶电泳后使用 dsGreen 凝胶染色溶液进行凝胶染色。

*Similarly to ethidium bromide, dsSafe when added in melted agarose solution can slightly reduce the mobility of nucleic acid sample in the gel.

*与溴化乙锭类似，dsSafe 在融化的琼脂糖溶液中添加时会略微降低核酸样品在凝胶中的迁移率。

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• dsGreen for Real-Time PCR, 100×

dsGreen for Real-Time PCR, 100×

dsGreen is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.

dsGreen 是一种非常灵敏的 dsDNA 检测染料。 dsDNA 的高灵敏度和高选择性允许使用 dsGreen 作为 qPCR 的通用 dsDNA 检测试剂。 无需使用标记探针来检测 dsGreen 的扩增——未标记的引物就足够了。

Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:

与 Lumiprobe 提供的其他用于凝胶染色的 dsGreen 制剂不同，该制剂专门设计用于实时 PCR 实验。 具体特点是：

• Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot
• 染料浓度针对 qPCR 进行了优化，并仔细调整了批次间可重复的结果
• PCR tested preparation – quality guaranteed
• PCR 检测制剂 – 质量保证
• Low fluorescence background – high fluorescence intensity gain
• 低荧光背景 – 高荧光强度增益

• Products
• DNA
• PCR/qPCR
• dsGreen Gel Staining Solution, 10000×

dsGreen Gel Staining Solution, 10000×

Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.

dsGreen 是一种灵敏的 dsDNA 结合染料，可用于琼脂糖和聚丙烯酰胺凝胶中的常规 DNA 检测。 还提供 qPCR 级试剂。
与溴化乙锭不同，dsGreen 对双链 DNA 具有高度选择性，危害更小，灵敏度更高。