Cosmo Bio抗体,Cosmo Bio,Anti Human/Rat Transmembrane Glycoprotein NMB (GPNMB) pAb (Rabbit, Purified Ig),CAC-ICA-TG1-RBP2

Application: FC, ELISA, IHC

Clonality: Polyclonal

Host: Rabbit

Purification: Ig-PA

Reactivity: Rat, Human

Lipid-laden macrophages may orchestrate pathology, an accepted notion for inborn lysosomal storage disorders (LSDs) and more recently for metabolic syndrome. The development of enzyme replacement therapy (ERT) for specific LSDs has led in the last decades to the identification of markers of lipid-laden macrophages. In LSDs characterized by foamy macrophages as storage cells, plasma GPNMB has been shown to accurately reflect disease burden. Moreover, GPNMB is also applicable in mouse models of LSDs like Gaucher disease and Niemann-Pick type C. GPNMB is also increased in several acquired diseases, such as metabolic syndrome and neurodegeneration. It therefore might be that these disease conditions share pathophysiological elements, in particular the accumulation of foamy, lysosomal stressed, macrophages. GPNMB is among the most upregulated proteins in lipid-laden macrophages. Nevertheless, at present its exact function in foamy macrophage remains largely enigmatic. Important unanswered questions concern the function(s) served by GPNMB, either the cellular membrane-bound or (extracellular) soluble isoforms, in lipid-laden macrophages and beyond. GPNMB is also expressed in melanocytes. GPNMB has been suggested to be involved in the delay of cell growth and regulation of metastasis. In recent years, Professor Hideaki Hara’s group at Gifu Pharmaceutical University, and others have shown that GPNMB expression is suppressed in mice that have developed amyotrophic lateral sclerosis (ALS), and tracking of GPNMB dynamics and function has been shown to be useful for ALS research.

Cosmo Bio抗体,Cosmo Bio,Anti CD63 Antigen (LAMP-3/Tspan-30) (Clone 8A12, TF5 Labeled),CAC-SHI-EXO-M02-TF5

Application: FC

Clonality: Monoclonal

Conjugation: Tide Fluor™5

Host: Mouse

Purification: Ig-PG

Reactivity: Human

Click here for more information and to see all exosome related products from Cosmo Bio USA.

CD63 (also known as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a cell surface glycoprotein which belongs to the tetraspanin superfamily. CD63 is known to complex with integrins. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cells and B cells. Importantly, it is found on the surface of exosomes.

Exosomes are cell-derived vesicles bounded by a lipid bilayer membrane and exhibiting a diameter of 50 to 150 nm. They are secreted from cultured cells and are observed in body fluids such as saliva, blood, urine, amniotic fluid, malignant ascites. Recent studies indicate that exosomes contain various proteins and RNAs, suggesting a role in information transfer between cells.

This monoclonal antibody can be used to immunoprecipitate exosomes from serum and culture supernatants.

References:
1) Yoshioka Y et al., Nat Commun. 2014 Apr 7;5:3591. doi: 10.1038/ncomms4591.
2) N Nishida-Aoki et al., Mol Ther. 2017 Jan 4;25(1):181-191. doi: 10.1016/j.ymthe.2016.10.009. 3) Saito S et al., Sci Rep. 2018 Mar 5;8(1):3997. doi: 10.1038/s41598-018-22450-2.

Cosmo Bio抗体,Cosmo Bio,Anti Thebaine mAb (Clone 5F7),CAC-KYU-HT-M002

Application: ELISA

Clonality: Monoclonal

Host: Mouse

Purification: Ig-PG

Within the fields of molecular biology and pharmacology, a small molecule is a low molecular weight (< 900 daltons) organic compound that may regulate a biological process, with a size on the order of 1 nm. Most drugs are small molecules. Larger structures such as nucleic acids and proteins, and many polysaccharides are not small molecules, although their constituent monomers (ribo- or deoxyribonucleotides, amino acids, and monosaccharides, respectively) are often considered small molecules. Small molecules may be used as research tools to probe biological function as well as leads in the development of new therapeutic agents. Some can inhibit a specific function of a protein or disrupt protein–protein interactions.

Pharmacology usually restricts the term “small molecule” to molecules that bind specific biological macromolecules and act as an effector, altering the activity or function of the target. Small molecules can have a variety of biological functions or applications, serving as cell signaling molecules, drugs in medicine, pesticides in farming, and in many other roles. These compounds can be natural (such as secondary metabolites) or artificial (such as antiviral drugs); they may have a beneficial effect against a disease (such as drugs) or may be detrimental (such as teratogens and carcinogens). [from: Wikipedia contributors. (2019, April 6). Small molecule. In Wikipedia, The Free Encyclopedia. Retrieved 19:49, May 29, 2019, from https://en.wikipedia.org/w/index.php?title=Small_molecule&oldid=891243496]

Thebaine is an alkaloid isolated from Papaver somniferum.

References:
1) Shoyama et al. (1993) Direct Determination Of Opium Alkaloid-Bovine Serum-Albumin Conjugate By Matrix-Assisted Laser-Desorption Ionization Mass-Spectrometry. Biol Pharm Bull. 16(10):1051-3. PMID: 8287039

Cosmo Bio抗体,Cosmo Bio,Anti Latency-Associated Peptide (LAP) Plasma Kallikrein Degradation Fragment L59 mAb (Clone 6D6),CAC-RIK-MA-L59

Application: ELISA, WB

Clonality: Monoclonal

Purification: Ig-PG

Reactivity: Human

Background
TGF-β is produced as a pro-protein in which the 25 kD active TGF-β is trapped by an N-terminal pro-peptide called Latency Associated Protein (LAP). Upon receipt of certain stimuli a conformational change is induced in a latent complex to release the active TGF-β from the complex. The resultant TGF-β binds to cognate signaling receptors and exerts various physiological and pathological activities. This reaction is called TGF-β activation reaction, which is known to be induced by binding of the latent complex to cell adhesion proteins such as thrombospondin and integrins, and/or by being cleaved by the action of proteases such as serine proteases, cysteine proteases, and MMPs in an organ and context-depending manner. The RIKEN Center for Biomedical Science and Research Center for Liver Cancer Prevention and Research Unit focused on the involvement of the serine protease plasmin and plasma kallikrein in the release and activation of TGF-β and its involvement in liver diseases. They showed that plasmin and plasma kallikrein cleave, respectively, at 56Lys-57Leu and 58Arg-59Leu within the LAP portion of the latent TGF-β1 molecule. The anti-TGF-β1 LAP-degradates (LAP-D) antibodies are useful to investigate the molecular mechanism of TGF-β activation and its related diseases including liver fibrosis/cirrhosis and liver degeneration.

Applications

1. Western Blotting
2. ELISA

Source

Professor Koichi, National Institute of Advanced Industrial Science and Technology RIKEN Biomedical Science Research Center Liver Cancer Prevention Research Unit

Product Description

References
1) LAP degradation product reflects plasma kallikrein-dependent TGF-β activation in patients with hepatic fibrosis, Hara M., Kirita A., Kondo W. et al. Springerplus. May 1; 3: 221. PMCID: PMC4033717 (2014).
2) L59 TGF-β LAP degradation products serve as a promising blood biomarker for liver fibrogenesis in mice, Hara M., Inoue I., Yamazaki Y. et al. Fibrogenesis Tissue Repair. Sep 15; 8: 17. PMCID: PMC4570586 (2015).

Cosmo Bio抗体,Cosmo Bio,Anti DNA Excision Repair Protein ERCC-1 (ERCC1) mAb (Clone E1-44),CAC-KUP-TM-M04

Application: WB

Clonality: Monoclonal

Host: Mouse

Purification: Ammonium Sulfate

Reactivity: Human

Nucleotide excision repair (NER) is a major repair system for removing a variety of DNA lesions including UV-induced cyclobutane pyrimidine dimers and (6-4) photoproducts as well as chemically-induced bulky base adducts. Defects in the NER system give rise to xeroderma pigmentosum (XP), an autosomal recessive disease characterized by a predisposition to skin cancer and in some cases neurological abnormalities. The early process of human NER, from damage recognition to dual incision (removal of damage-containing oligonucleotides), is accomplished by six core NER factors, XPC-RAD23B, TFIIH, XPA, RPA, XPF-ERCC1 and XPG.

ERCC1 forms a stable complex with XPF and the heterodimer has an ability to make a nick on the DNA strand which makes the transition from duplex to single-stranded DNA in the 5′ to 3′ direction. In the NER process, ERCC1-XPF complex is responsible for 5′-incision at a dual incision step.

Cosmo Bio抗体,Cosmo Bio,Anti Cyclin-Dependent Kinase 5 Activator 1 (p35) – phospho Ser8 pAb (Rabbit, Affinity Purified),CAC-SDT-02-P35

Application: WB

Clonality: Polyclonal

Host: Rabbit

Purification: Purified – Affinity

Reactivity: Mouse, Rat, Human

Cyclin-dependent kinase 5 (Cdk5) is a unique member of the Cdk family. Its activity in postmitotic neurons is completely dependent upon association with one of two neuronal specific activators, p35 or p39. Cdk5/p35 is involved in a panoply of processes critical to central nervous system function both during development and throughout maturity including neuronal migration during corticogenesis, neurite outgrowth, regulation of the synaptic vesicle cycle, neurotransmitter release, and postsynaptic neurotransmitter receptor regulation and signaling. The mechanisms by which Cdk5 activity is normally regulated remains to be fully delineated. Furthermore, because aberrant Cdk5 activity has been implicated in the etiology of neurodegenerative diseases, identifying the biochemical mechanisms contributing to deregulation of Cdk5 is of substantial biomedical relevance.

Deregulation of Cdk5 results from removal of the first 98 amino acids of p35 by the Ca2-dependent cysteine protease, calpain, leaving Cdk5 associated with the N-terminal truncated form p25. Cleavage of p35 to p25 changes the subcellular distribution of active Cdk5 from membranes to the cytosolic fraction, thereby altering substrate specificity. p25 accumulates in neurons undergoing various types of cell death. Expression of Cdk5/p25 in cultured cells results in increased phospho-Tau levels in comparison to cells expressing Cdk5/p35. Furthermore, exogenous overexpression of p25 in transgenic mice results in a neurodegenerative phenotype including the formation of paired helical filaments, Tau aggregation, and neuronal loss similar to that observed in Alzheimer disease.

Ca2-dependent activation of the cytoplasmic protease calpain is involved in apoptotic and necrotic cell death. Calpain generally recognizes motifs between conformational domains and cleaves substrate proteins in a limited manner, although the physiological function of calpain activity remains unclear. In some cases calpain cleavage is suspected to be a signaling process. Calpain-mediated cleavage of many proteins including neurofilament proteins, alpha II-spectrin (20), NR2 subunits of N-methyl-D-aspartic acid receptors, and ezrin is suppressed through phosphorylation. However, how such signaling works and the mechanisms of phosphorylation-dependent inhibition are unknown.

Previously we demonstrated that Cdk5 phosphorylated p35, that p35 occurred as a phosphoprotein in neurons, and that the phosphorylation state of p35 affected its susceptibility to calpain cleavage. Phospho-p35 predominates in fetal rat brain and is resistant to the cleavage by calpain, whereas unphosphorylated p35 present during adulthood is more vulnerable to calpain-dependent cleavage. Here we report that Ser8 and Thr138 of p35 serve as the sites of Cdk5-dependent phosphorylation. Furthermore, phosphorylation at these sites reduces the susceptibility of p35 to calpain cleavage. Moreover, specific dephosphorylation of Thr138 increases the susceptibility of p35 to cleavage by calpain in adult rat brains, suggesting that phosphorylation of this site is a particularly critical determinant of Cdk5-dependent neuronal cell death in neurodegenerative diseases.

[from: Kamei, H., Saito, T., Ozawa, M., Fujita, Y., Asada, A., Bibb, J.A., Saido, T.C., Sorimachi, H. and Hisanaga, S. Suppression of Calpain-dependent Cleavage of the CDK5 Activator p35 to p25 by Site-specific Phosphorylation J. Biol. Chem. 282(3): 1687-1694 (2007)]

Cosmo Bio抗体,Cosmo Bio,High Sensitivity 6-4 Photoproducts (6-4PPs) ELISA Kit,CSR-NM-MA-K002

Prolonged exposure to solar UV radiation may result in harmful acute and chronic effects to the skin (including skin cancers), eye, and immune system. These harmful effects appear to be closely related to UV-induced DNA damage. Indeed, UV-induced DNA damage plays significant roles in cell-cycle arrest, activation of DNA repair, cell killing, mutation, and neoplastic transformation. The major types of DNA damage induced by solar UV radiation are cyclobutane pyrimidine dimers (CPDs), (6–4) photoproducts (6-4PPs), and Dewar valence isomers of 6-4PPs (Dewar photoproducts; DewarPPs) formed between adjacent pyrimidine nucleotides on the same DNA strand. Approximately 70-80% of UV-induced DNA damage is CPDs and the remaining is 6-4PPs and Dewar isomers of 6-4PPs. DewarPPs are produced by the photoisomerization of 6-4PPs by UV radiation around 325 nm. In normal human cells these types of DNA lesions are repaired by nucleotide excision repair (NER).

To better study molecular events surrounding UV-induced DNA damage and repair, Mori et al. previously developed and characterized monoclonal antibody (mAb) specific for CPDs and mAb specific for 6-4PPs (1) while Matsunaga et al. developed and characterized mAb specific for DewarPPs (2). Three of these antibodies (CPDs: clone TDM-2; 6-4PPs: clone 64M-2; DewarPPs: clone DEM-1) continue to be cited frequently in the literature, often for use in ELISA.

This High Sensitivity (6-4)photoproducts (6-4PPs) ELISA Kit is the only commercially available ELISA utilizing anti- 6-4PPs clone 64M-2 and has been optimized for high sensitivity detection of 6-4PPs in DNA purified from cultured cells or from skin epidermis. This ELISA detects 6-4PPs from dipyrimidines in all DNA sequence contexts (i.e., TT, TC, CT and CC). Thus, the availability and convenience of this ELISA Kit will contribute to further understanding molecular mechanisms involved in cellular responses to UV radiation and DNA damage with applications across many research fields including cancer research, photobiology, dermatology, ophthalmology, immunology, and cosmetics science.

Cosmo Bio抗体,Cosmo Bio,Anti TAR DNA-Binding Protein 43 (TDP43), phospho Ser409 pAb (Rabbit, Antiserum),CAC-TIP-PTD-P03

Anti-TAR DNA-Binding Protein 43 (TDP-43), phospho Ser409 pAb was prepared from rabbits immunized with phospho-peptide [CMDSKS(p)SGWGM]. This pAb recognizes human TDP-43 phosphorylated on serine 409 and is validated for western blot, ELISA and IHC(f) analyses of TDP-43 proteinopathy.

TDP-43, a heterogeneous nuclear ribonucleoprotein, was identified as a component of ubiquitin-positive and tau-negative inclusions observed in cases of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Immunochemical analyses using antibodies generated against phospho- and non-phosphopeptides of human TDP-43 revealed that abnormally phosphorylated full-length TDP-43 (45 kDa), C-terminal fragments (~25 kDa) and smearing substances are deposited as intracellular inclusions in affected regions of FTLD-U and ALS cases. This antibody is a powerful tool for biochemical and immunohistochemical analyses of neurodegenerative diseases and for evaluation of cellular or animal models of TDP-43 proteinopathy.

Specifications

• Product type                primary antibody
• Immunogen                 CMDSKS(p)SGWGM, S(p): phosphoserine 409
• Raised in                     rabbit
• Source                         serum
• Form                           liquid antiserum with 0.05% NaN3 as a preservative
• Volume                       100 uL
• Label                           unlabeled
• Specificity                   phospho-TDP-43
• Cross reactivity           human
• Storage                        below -20°C and below -70°C for prolonged storage; aliquot to avoid freeze/thaw cycles.

Recommended dilutions

• Western blotting                     1/1000-1/5000
• Immunohistochemistry           1/1000-1/5000
• ELISA                         1/1000-1/5000
• Other applications not tested
• Optimal dilutions/concentrations should be determined by end user.

References

1. Inukai Y, Nonaka T, Arai T, et al. Abnormal phosphorylation of Ser409/410 of TDP-43 in FTLD-U and ALS. FEBS Lett. 582, 2899-2904, 2008 PubMed: 18656473

Cosmo Bio抗体,Cosmo Bio,Anti Myc Proto-Oncogene Protein (c-Myc) mAb (Clone 9E10),CAC-XIM-MA001

Application: FC, IP, IHC, WB, ChIP, ELISA

Clonality: Monoclonal

Host: Mouse

Purification: Ig-PG

Reactivity: Human

c-Myc is a very potent oncogene upregulated in various types of cancer. c-Myc, through its role as a transcription factor, alters the expression of hundreds of target genes, often oncogenes or tumor suppressors. Clone 9E10 recognizes recombinant proteins that incorporate the c-Myc epitope tag and detects human c-Myc proteins and peptides in random coil rather than alpha-helix structures.

References:
1) Mateos-Gomez et al. 2015. Nature. 518(7538):254-7. PMID: 25642960. (WB)
2) Zhang et al. 2014. Genes Dev. 28(8):829-34. PMID: 24736842. (ChIP)
3) Zhou et al. 2012. J Cell Biol. 196(2):203-11. PMID: 22270916. (IP, WB)
4) Mendes et al. 2010. J Biol Chem. 285(50):39117-26. PMID: 20937822. (IP, WB)
5) Royds et al. 1992. J Pathol. 166(3):225-33. PMID: 1381423. (IHC)
6) Spandidos et al. 1987. Anticancer Res. 7(6):1299-304. PMID: 3327455. (IHC)
7) Kari et al. 1986. J Virol. 60(2):345-52. PMID: 3021969. (IP)
8) Evan et al. 1985. Mol Cell Biol. 5(12):3610-6. PMID: 3915782. (WB)

Cosmo Bio抗体,Cosmo Bio,Anti Insulin-Like Growth Factor-Binding Protein 7 (IGFBP-7/IGFBP-rP1) mAb (Clone 88),CAC-YCU-MK-TF01

Application: ELISA, IHC(p), WB

Clonality: Monoclonal

Host: Mouse

Purification: Purified – Affinity

Reactivity: Human

The insulin-like growth factors (IGFs) are proteins with high sequence similarity to insulin. IGFs are part of a complex system that cells use to communicate with their physiologic environment. This complex system (often referred to as the IGF “axis”) consists of two cell-surface receptors (IGF1R and IGF2R), two ligands (Insulin-like growth factor 1 (IGF-1) and Insulin-like growth factor 2 (IGF-2)), a family of seven high-affinity IGF-binding proteins (IGFBP1 to IGFBP7), as well as associated IGFBP degrading enzymes, referred to collectively as proteases. [from: Wikipedia contributors. (2019, May 23). Insulin-like growth factor. In Wikipedia, The Free Encyclopedia. Retrieved 17:42, June 4, 2019, from https://en.wikipedia.org/w/index.php?title=Insulin-like_growth_factor&oldid=898477681] Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene.[5][6][7] The major function of the protein is the regulation of insulin-like growth factor (IGF) availability in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity.[8] It also stimulates cell adhesion. The protein is implicated in some cancers.[9] [adapted from: Wikipedia contributors. (2019, April 15). IGFBP7. In Wikipedia, The Free Encyclopedia. Retrieved 17:29, June 4, 2019, from https://en.wikipedia.org/w/index.php?title=IGFBP7&oldid=892525075]

References:
1) Koike A., Uematsu Y., Bonkobara M., Yamaguchi T., Washizu T., Arai T. (2007) 144th Annual Meeting of Japanese Society of Veterinary Science, I-39.
2) Uematsu Y, Yamaguchi T, Koike A, Yagihara H, Hasegawa D, Matsuki N, Ono K, Washizu T, Arai T, Bonkobara M. (2008) Generation of Monoclonal Antibody against Canine Neural-Cell Adhesion Molecule. Journal of Veterinary Medical Science. 70(8):845-847

Cosmo Bio抗体,Cosmo Bio,Anti Inter-Alpha-Trypsin Inhibitor Heavy Chain H4 (ITIH4) pAb (Sprague Dawley Rat, Antiserum),CAC-ICA-TG2-RTP2

Application: FC, ELISA, IHC

Clonality: Polyclonal

Host: Rat

Purification: Serum

Reactivity: Rat, Human

Neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease have been increasing rapidly and have become a serious social problem. In recent years, new causative genes have been discovered for amyotrophic lateral sclerosis (ALS) and other intractable neurological diseases opening new avenues for research on pathogenesis. It has been suggested that aggregation and accumulation of specific proteins cause neurotoxicity and the formation of lesions, but the onset and progression mechanisms are still unclear. Neuropathological diagnostic and experimental model biomarkers are needed for drug construction, drug discovery, and therapeutic development.

ITIH4 is secreted into the blood and is cleaved by plasma kallikrein into two protein fragments. The expression of this protein is localized to the liver, and peptide fragments of ITIH4 are detected in the serum of patients with liver cancer and cirrhosis. Thus, ITIH4 is a new biomarker for liver disease. Furthermore, experiments using model mice with amyotrophic lateral sclerosis (ALS) have shown that ITIH4 elevation promotes progression of pathological conditions. On the other hand, ITIH4 has many sites that undergo post-translational modification such as glycosylation, and its application to cell staining and flow cytometry by antibodies using peptides and recombinant proteins as antigens has been considered extremely difficult. These problems have been solved by the nanotaxi method, and we present two rat polyclonal antibodies suitable for detection of human ITIH4.

Cosmo Bio抗体,Cosmo Bio,Anti Developmental Pluripotency-Associated Protein 4 (Dppa4) pAb (Rabbit, Ammonium Sulfate Purified),CAC-TMD-PB-DP4

Application: ICC, WB, IF

Clonality: Polyclonal

Host: Rabbit

Purification: Ammonium Sulfate

Reactivity: Mouse

Pluripotency is maintained through a complex network of signaling pathways that interact with transcription factors including octamer binding protein 4, SRY-box 2, and Nanog, chromatin modifying complexes, microRNAs, and the DNA structure itself to preserve the undifferentiated, self-renewing state. Whereas the signaling requirements for the maintenance of stem cells can vary by species of origin, state of the stem cells, and culturing conditions, the core transcriptional circuity activated and maintained by these pathways is highly conserved. The self-sustaining nature of the pluripotency network is evident in the finding that somatic cells can be reprogrammed to a pluripotent state through the transient introduction of a small group of factors, which serve to reestablish the stem cell signaling and transcriptional network. Growing understanding of the molecular circuitry of pluripotency has major implications for the field’s view of developmental biology and disease pathogenesis and is important for the evolving field of regenerative medicine. [from: Rachel H. Klein and Paul S. Knoepfler. “The Molecular Circuitry Underlying Pluripotency in Embryonic and Induced Pluripotent Stem Cells.” Principles of Regenerative Medicine 3rd Edition, edited by Anthony Atala, Robert Lanza, Antonios G. Mikos and Robert Nerem, Academic Press, 2019, pp. 49-63.]

DPPA4 (developmental pluripotency associated 4) is a highly expressed gene in early embryos and ES cells. DPPA4 binds to transcriptionally active chromatin and suppresses the differentiation of ES cells into primitive ectoderm (future endoderm).

Source: Professor Hirofumi Teraoka, Department of Pathology and Biochemistry, Research Institute for Intractable Diseases, Tokyo Medical and Dental University

References:
Masaki H, Nishida T, Kitajima S, Asahina K, and Teraoka H.: J. Biol. Chem. 282, 33034-33042 (2007).

Cosmo Bio抗体,Cosmo Bio,Anti N-Syndecan (SDC3) pAb,CAC-NU-07-004

Application: IHC(p), WB, IF

Clonality: Polyclonal

Host: Rabbit

Purification: Serum

Reactivity: Mouse, Rat

Syndecan is the family name of transmembrane proteoglycans that carry predominantly heparan sulfate side chains. This proteoglycan family consists of four members. N-syndecan (syndecan-3) is the principal member expressed during early postnatal development in both central and peripheral nervous systems. N-syndecan binds various heparin-binding growth factors such as FGFs via the heparan sulfate moiety, and communicates with the cytoskeleton via the cytoplasmic domain of the core protein. N-syndecan plays a pivotal role in formation of the neural network through these molecular interactions. This antibody recognizes effectively the core protein of N-syndecan.

References:
1) Watanabe, E., Matsui, F., Keino, H., Ono, K., Kushima, Y., Noda, H.. & Oohira, A., A membrane-bound heparan sulfate proteoglycan that is transiently expressed on growing axons in the rat brain, (1996) J. Neurosci. Res., 44, 84-96.
2) Toba, Y., Horie, M., Sango, K., Takashiki, A., Matsui, F., Oohira, A., & Kawano, H., Expression and immunohistochemical localization of heparan sulfate proteoglycan N-syndecan in the migratory pathway from the rat olfactory placode, (2002) Eur. J. Neurosci., 15, 1-13.

Cosmo Bio抗体,Cosmo Bio,Anti Inhibin Beta A Chain (EDF) mAb,CAC-KZ-HS-M02

Application: WB, IHC

Clonality: Monoclonal

Host: Mouse

Purification: Ascities

Reactivity: Human, Mouse, Rat, Bovine, Sheep

Cosmo Bio抗体,Cosmo Bio,Anti L-Ascorbate Peroxidase 1, Cytosolic (AP/AtAPx01) pAb (Rabbit, Ammonium Sulfate Purified),CAC-SDT-01-APX

Application: IHC, WB

Clonality: Polyclonal

Host: Rabbit

Purification: Ammonium Sulfate

Reactivity: Plant, Maize, Rice

Because plants have a sessile lifestyle, they must adjust to numerous external stimuli and coordinate their growth and development accordingly. The plant hormones, a group of structurally unrelated small molecules, are central to the integration of diverse environmental cues with a plant’s genetic program. The ‘classical’ phytohormones, identified during the first half of the twentieth century, are auxin, abscisic acid, cytokinin, gibberellin and ethylene. More recently, several additional compounds have been recognized as hormones, including brassinosteroids, jasmonate, salicylic acid, nitric oxide and strigolactones. Plants also use several peptide hormones to regulate various growth responses, but this class of hormones is beyond our scope here. With the application of genetic approaches, mainly in Arabidopsis thaliana, many aspects of hormone biology have been elucidated. Most hormones are involved in many different processes throughout plant growth and development. This complexity is reflected by the contributions of hormone synthesis, transport and signaling pathways, as well as by the diversity of interactions among hormones to control growth responses.

Genetic screens resulted in the identification of many of the proteins involved in hormone signaling and the analysis of these proteins has contributed significantly to our current models of hormone action. One particularly exciting outcome is the recent identification of receptors for auxin, gibberellin, jasmonate and abscisic acid. Though far from complete, our improved understanding of hormone perception and signaling has allowed for comparisons between hormones. From these it is clear that some hormones (cytokinins, ethylene and the brassinosteroids) use well-characterized signaling mechanisms. On the other hand, the identification and characterization of the auxin and jasmonate receptors, as well as proteins in gibberellin signaling, have highlighted a novel mechanism for hormone perception in which the ubiquitin–proteasome pathway has a central role. [from: Santner A., Mark E., Recent advances and emerging trends in plant hormone signalling (2009) Nature 459: 1071-1078]

Ascorbate peroxidase (or APX) is a member of the family of heme-containing peroxidases. Ascorbate-dependent peroxidase activity was first reported in 1979, more than 150 years after the first observation of peroxidase activity in horseradish plants and almost 40 years after the discovery of the closely related cytochrome c peroxidase enzyme. Peroxidases have been classified into three types (class I, class II and class III). Ascorbate peroxidase is a class I peroxidase enzyme. APXs catalyse the H2O2-dependent oxidation of ascorbate in plants, algae and certain cyanobacteria. APX has high sequence identity to cytochrome c peroxidase, which is also a class I peroxidase enzyme. Under physiological conditions, the immediate product of the reaction, the monodehydroascorbate radical, is reduced back to ascorbate by a monodehydroascorbate reductase (monodehydroascorbate reductase (NADH)) enzyme. APX is an integral component of the glutathione-ascorbate cycle.

Source: Professor Koichi Koshiba, Tokyo Metropolitan University Graduate School of Science and Technology Department of Life Science

References:
Koshiba, T. (1993) Cytosolic ascorbate peroxidase in seeds and leaves of maize (Zea mays). Plant Cell Physiol. 34: 713-721.

Cosmo Bio抗体,Cosmo Bio,Anti Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A/LC3) mAb (Clone LC3.No.6),CAC-CTB-LC3-1-50

Application: WB

Clonality: Monoclonal

Host: Mouse

Purification: Ig-PG

Reactivity: Mouse, Human

LC3, a mammalian homolog of yeast Atg8, is used as a marker for autophagic vesicles (autophagosomes) formed in the process of autophagy. Immediately after synthesis, LC3 is cleaved by Atg4 to form LC3-I. When autophagy is induced phosphatidylethanolamine is covalently linked to the C-terminal glycine of LC3-I to form membrane-bound LC3-II. Most LC3-II is thought to be present in autophagosomal membranes. Since autophagosomes are degraded by fusion with lysosomes, LC3-II itself is also degraded by autophagy. Therefore, it is generally accepted that the amount of LC3-II correlates well with the amount of autophagosomes. This antibody reacts with both LC3 isoforms (LC3-I and LC3-II) and shows good results in Western blot experiments.

References:

1) Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y. and Yoshimori, T. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing EMBO J. 19, 5720-5728. (2000).

2) Mizushima, N., Yoshimori, T. How to interpret LC3 immuno

半糖粒是细胞与细胞外基质之间的黏附结构。它们在将中间纤维锚定在细胞外基膜上发挥作用。在结构上，半脂质体有两种形式:I型和II型。I型半脂质体发育于分层上皮，如表皮。其主要成分包括细胞内连接蛋白果胶和BPAG1，粘附受体整合素α6β4和胶原型BP180/XVII。II型半脂质体作为I型半脂质体的简化形式出现在血管、雪旺细胞和消化道上皮中，仅由果胶和整合素α6β4组成。半糖体粘附受体通常与基底膜上的层粘连蛋白5相关。此外，层粘连蛋白5(其中层粘连蛋白2是一个亚基)通过VII型胶原蛋白与真皮层中的胶原纤维相连。半脂糖体相关蛋白的遗传缺失导致各种形式的大疱性表皮松解症，突出了它们在促进表皮和基底膜之间粘附的重要性。

果胶将中间丝与微管和微丝相连，并将中间丝固定在桥粒或半桥粒上。也可以将肌动蛋白等肌肉蛋白结合到肌肉的膜复合物上。可能不仅与丝状网络有关，而且与丝状网络的动态调节有关。肌肉的结构组成部分。异构体9在维持肌纤维完整性方面起着重要作用。

Cosmo Bio抗体,Cosmo Bio,Anti Plectin (PCN/PLTN) mAb (Clone PN753),CAC-NU-01-PLN

Application: IP, IF, WB

Clonality: Monoclonal

Host: Mouse

Purification: Supernatant

Reactivity: Porcine, Rabbit, Rat, Human

Hemidesmosomes are adhesive structures between cells and the extracellular matrix. They play a role in anchoring intermediate fibers to the extracellular basement membrane. Structurally, hemidesmosomes occur in two forms: Type I and Type II. Type I hemidesmosomes develop in stratified epithelia such as the epidermis. Its main components include the intracellular linker proteins Plectin and BPAG1, the adhesion receptor integrin α6β4 and collagen type BP180/XVII. Type II hemidesmosomes occur in blood vessels, Schwann cells, and digestive tract epithelia as a simplified form of Type I hemidesmosomes, consisting only plectin and integrin α6β4. The hemidesmosomal adhesion receptor is normally associated with Laminin 5 in the basement membrane. Furthermore, Laminin 5 (of which Laminin gamma 2 is a subunit) is linked to collagen fibers in the dermis via type VII collagen. Genetic deletion of hemidesmosome-related proteins causes various forms of epidermolysis bullosa, highlighting their importance in promoting adhesion between the epidermis and the basement membrane.

Plectin interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.

References:

1) Yamauchi T., et al. J. Dermatol. Sci., 76:25-33 (2014).

2) Hirako Y., et al. Exp. Cell Res., 324:172-182 (2014).

3) Hirako Y., et al. J. Biol. Chem., 273:9711-9717 (1998).