- Lumiprobe NIR fluorescent dyes-Near Infrared (NIR) Fluorescent Dyes近红外荧光染料
- NIR fluorescent dyes
Near-infrared fluorescent dyes are well suited for in vivo imaging applications.
Near Infrared (NIR) Fluorescent Dyes近红外荧光染料
Although most fluorophores operate in visible or ultraviolet parts of the spectrum, the near-infrared (NIR) area is very promising for fluorescence detection and imaging.
In infrared, much less background fluorescence is observed on microarrays and tissue samples. This makes NIR dyes an ideal choice for cases where low background and high signal-to-noise ratio are critical.
Biological tissues possess a “near-infrared window” of transparency above 700 nm (actual value depends on tissue type). This allows for in-depth imaging of organisms in a real-time, non-destructive fashion. Labeling a biomolecule (peptide, protein, or other) with NIR dye makes it possible to track it and study the distribution of the molecule in an organism. This type of imaging is an excellent tool for life science and drug design.
Lumiprobe offers two near-infrared fluorophores: Cy7, and Cy7.5.
The dyes belong to the cyanine family and possess high extinction coefficients. The internal polymethine chain contains a cyclohexane moiety to increase rigidity and quantum yield. Fluorescence quantum yield is thus improved by 20% compared to the parent structures.
虽然大多数荧光光在光谱的可见或紫外部分运行,但近红外(NIR)区域在荧光检测和成像方面很有前途。
在红外线中,在微阵列和组织样品上观测到的背景荧光要少得多。这使NIR染料成为一个理想的选择,在低背景和高信噪比是关键的情况下。
生物组织拥有一个透明度在700纳米以上的”近红外窗口”(实际值取决于组织类型)。这就可以实时、无破坏性地对生物体进行深入成像。用NIR染料标记一个生物分子(肽、蛋白质或其他)使追踪它并研究分子在有机体中的分布成为可能。这种类型的成像是生命科学和药物设计的一个很好的工具。
激光探测器提供两种近红外荧光光: Cy7 ,以及 Cy7.5 .
染料属于氰基,具有较高的消光系数。内部聚甲基链中含有一部分乙烷,以增加刚度和量子屈服度。因此,荧光量子产率比父结构提高20%。
| Cy7 | Cy7.5 | |
|---|---|---|
| Excitation wavelength | 750 nm | 788 nm |
| Extinction at λmaxex | 199,000 | 223,000 |
| Emission wavelength | 773 nm | 808 nm |
| Stokes shift | 23 nm | 20 nm |
| Availability | NHS ester (amine-reactive) NHS ester, water soluble (amine-reactive) Maleimide (thiol-reactive) Maleimide, water soluble (thiol-reactive) Hydrazide (carbonyl-reactive) Primary amine Primary amine, water soluble Carboxylic acid Carboxylic acid, water soluble Azide (for сlick chemistry) Azide, water soluble (for сlick chemistry) Alkyne (for сlick chemistry) Alkyne, water soluble (for сlick chemistry) Tetrazine (for сlick chemistry) DBCO (for сlick chemistry) |
NHS ester (amine-reactive) NHS ester, water soluble (amine-reactive) Maleimide (thiol-reactive)Hydrazide (carbonyl-reactive) Primary amine Carboxylic acid |
Succinimide esters are most useful for labeling amino groups in various biomolecules, such as proteins and peptides. Azides and alkynes are reactive dyes for advanced and efficient click chemistry reaction. We also recommend preparing NIR dye-labeled oligonucleotides through post-synthetic click chemistry labeling alkynylated oligos with dye azides.
Absorption spectra
Emission spectra