- Description
Catalogue number
CYT-1196
Synonyms
Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734
Introduction
Insulin-like growth factor II is an important foetal growth hormone.IGF-II is made by theca cells during gestation. IGF-II binds the sink IGF-II receptor which results in IGF-II degradation.IGF-II also engages the IGF-I receptor to arbitrate embryonic growth.
Description
IGF2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, monomeric polypeptide chain containing 67 amino acids and having a total molecular mass of 7.4kDa.
The IGF2 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Formulation
Lyophilized from a sterile filtered solution containing 0.1 % trifluoroacetic acid .
Solubility
It is recommended to reconstitute the lyophilized IGF2 in sterile water at a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized IGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF2 Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein . Please prevent freeze-thaw cycles.
Purity
Amino acid sequence
AYGPGETLCG GELVDTLQFV CSDRGFYFSR PSSRANRRSR GIVEECCFRS CDLALLETYC ATPAKSE
Biological Activity
The ED50 as determined by FDC-P1 cell proliferation is ≤ 50 ng/mL, corresponding to a specific activity of ≥ 2.0 x 10^4 units/mg.
Usage
Related Products
-
IGF2 Human