ProSpec-SHH Human, His/CYT-717

SHH, HHG-1, HHG1, Sonic hedgehog protein, TPT, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5.

Recombinant Human Sonic Hedgehog is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. 3 mammalian hedgehog genes share about 60% homology. The Human Sonic Hedgehog is 99% homologous to the mouse gene. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the

重组人声波刺猬是一小部分分泌蛋白的一部分，这些蛋白对脊椎动物和无脊椎动物的发育都至关重要。 3个哺乳动物刺猬基因具有约60%的同源性。 人类声波刺猬与小鼠基因有 99% 的同源性。 Sonic HedgeHog 是一种对引导早期胚胎至关重要的蛋白质。 它被认为是腹侧神经管、前后肢轴和腹侧体节的主要诱导信号。 Sonic HedgeHog 与修补受体结合，该受体与 Smoothened 相关联，以激活靶基因的转录。 在没有声波 HedgeHog 的情况下，修补受体抑制了平滑的本构信号活性。 Sonic HedgeHog 还调节另一个因素，即 gli 癌基因。 Sonic HedgeHog 细胞间信号对于发育过程中的各种模式事件至关重要：脊索产生的信号可诱导腹侧细胞命运

Sonic HedgeHog Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 183 amino acids and having a molecular mass of 20.7kDa. The Sonic HedgeHog is fused to 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Sonic HedgeHog Recombinant Human 是一条单一的、非糖基化的多肽链，含有 183 个氨基酸，分子量为 20.7kDa。 Sonic HedgeHog 在 C 端与 8 个氨基酸 His-Tag 融合，并通过专有色谱技术进行纯化。

Escherichia Coli.

Sterile filtered colorless solution.

SHH Human solution containing 20mM Tris HCL pH-8, 0.1M NaCl, & 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein . Please prevent freeze thaw cycles.

Greater than 95.0% as determined by -PAGE.

MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKISRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGGLEHHH HHH.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.