ProSpec-IFN b 1b Human/CYT-234

Leukocyte IFN, B cell IFN, Type I IFN, IFNB1, IFB, IFF, IFNB, IFN-b 1b, MGC96956.

IFN-beta 1b has antiviral, antibacterial and anticancer activities.

IFN beta 1b Human Recombinant produced in E.Coli is a single, non-glycosylated mutein of human IFN beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18510.86 Dalton.
The IFN-beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17.
IFN beta-1b is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 IFN beta 1b Human Recombinant 是人 IFN beta-1b 多肽链的单一非糖基化突变蛋白，含有 165 个氨基酸，分子量为 18510.86 Dalton。
IFN-β 基因是从人成纤维细胞中克隆出来的，并被改变为用丝氨酸代替 17 位发现的半胱氨酸残基。
IFN beta-1b 是通过专有的色谱技术纯化的。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from 1mg/ml solution containing 50mg Human Albumin & 50mg dextrose.

It is recommended to reconstitute the lyophilized IFN beta-1b in sterile 18M-cm H2O at a concentration of 0.25mg/ml, which can then be further diluted to other aqueous solutions.

建议在无菌 18M-cm H2O 中以 0.25mg/ml 的浓度重构冻干的 IFN beta-1b，然后可以将其进一步稀释成其他水溶液。

Lyophilized IFN-beta 1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFNB 1b should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

冻干的 IFN-beta 1b 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，IFNB 1b 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
请防止冻融循环。

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.

The specific activity as determined in a viral resistance assay was found to be 32 x 10⁶ IU/mg.

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a calibrated solution of IFN-beta as a Reference Standard.

蛋白质定量通过两种独立的方法进行：
1. 280 nm 的紫外光谱，使用 1.493 的吸光度值作为 0.1% 溶液的消光系数。 该值是由 PC GENE 计算机蛋白质序列分析程序计算得出的。
2. 通过 RP-HPLC 分析，使用校准的 IFN-β 溶液作为参考标准。

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。