ProSpec-SAA1 Human/CYT-787

Serum amyloid A protein, SAA, Amyloid protein A, Amyloid fibril protein AA, SAA1, SAA2, PIG4, TP53I4, MGC111216.

SAA1 protein is an acute phase apolipoprotein reactant which is produced mostly by hepatocytes and under regulation of inflammatory cytokines. SAA1 protein is produced mainly in the liver and circulates in low levels in the blood. The SAA1 seems to have a role in the immune system. SAA1 protein levels increase in the blood and other tissues under conditions of inflammation. SAA1 may facilitate the repair of injured tissues; it also acts as an antibacterial agent, and signals the migration of germ-fighting cells to sites of infection. SAA1 also functions as an apolipoprotein of the HDL complex.
Elevated levels of SAA1 ultimately affect secondary amyloidosis, extracellular amassing of amyloid fibrils, resulting from a circulating precursor, in a variety of tissues and organs. The most widespread type of amyloidosis appears secondary to chronic inflammatory disease, mainly rheumatoid arthritis. The SAA1 cleavage product a designated amyloid protein A is deposited systemically as amyloid in vital organs such as the liver, spleen, and kidneys in chronic inflammatory diseases patients. These deposits are extremely insoluble and resistant to proteolysis; they disrupt tissue structure and compromise performance.

SAA1蛋白是一种急性期载脂蛋白反应物，主要由肝细胞产生，在炎性细胞因子的调节下。 SAA1 蛋白主要在肝脏中产生，并在血液中以低水平循环。 SAA1 似乎在免疫系统中起作用。在炎症条件下，血液和其他组织中的 SAA1 蛋白水平会升高。 SAA1 可促进受损组织的修复；它还可以作为一种抗菌剂，并发出抗细菌细胞迁移到感染部位的信号。 SAA1 还作为 HDL 复合物的载脂蛋白发挥作用。
SAA1 水平升高最终会影响多种组织和器官中的继发性淀粉样变性、淀粉样蛋白原纤维的细胞外聚集，这是由循环前体引起的。最普遍的淀粉样变性类型似乎继发于慢性炎症性疾病，主要是类风湿性关节炎。 SAA1 裂解产物称为淀粉样蛋白 A 以淀粉样蛋白的形式全身沉积在慢性炎性疾病患者的肝脏、脾脏和肾脏等重要器官中。这些沉积物极难溶解，对蛋白水解有抵抗力；它们会破坏组织结构并损害性能。

SAA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 104 amino acids and having a molecular mass of 11.7kDa.
The SAA1 is purified by proprietary chromatographic teichnques.

在大肠杆菌中产生的 SAA1 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 104 个氨基酸，分子量为 11.7kDa。
SAA1 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

SAA1 was lyophilized from a 0.2µm filtered concentrated solution in 20mM Tris-HCl, pH 9.0 and 150mM NaCl.

It is recommended to reconstitute the lyophilized SAA1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 SAA1，然后可以进一步稀释成其他水溶液。

Lyophilized SAA1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SAA1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 SAA1 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，SAA1 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISD ARENIQRFFG HGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY.

The biological activity determined by a chemoattract bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

通过使用人单核细胞的化学吸引生物测定法确定的生物活性在 10-100 ng/ml 的浓度范围内。

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.