ProSpec-TFF3 Human, His/CYT-616,Trefoil Factor-3 Human Recombinant, His Tag

TFF-3, ITF, TFI, HITF, hP1.B, TFF3, Trefoil factor 3, Intestinal trefoil factor.

Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, relatively through an epidermal growth factor receptor-dependent pathway. TFF3 overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 is normally expressed in hepatocellular carcinoma and its expression associates with tumor grade.

TFF 家族的蛋白质的特征在于获得至少 1 个三叶基序拷贝，这是一个包含 3 个保守二硫键的 40 个氨基酸结构域。 三叶因子是在胃肠道粘膜中表达的稳定分泌蛋白，可保护粘膜免受损伤，稳定粘液层并影响上皮的愈合。TFF2 抑制胃酸运动和分泌。 TFF2 通过与碳水化合物侧链的相互作用稳定粘液凝胶中的糖蛋白。 TFF3 相对通过表皮生长因子受体依赖性途径诱导纤毛发生并促进气道上皮纤毛细胞分化。 TFF3 过表达对于小鼠和人类肝细胞癌发生的进展至关重要。 TFF-3通常在肝细胞癌中表达，其表达与肿瘤分级相关。

TFF3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids which includes a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 7.82 kDa.
TFF3 Human Recombinant is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TFF3 Human Recombinant 是一个单一的、非糖基化的多肽链，含有 69 个氨基酸，其中包括一个在 N 端融合的 10 个氨基酸的 His 标签，总分子量为 7.82 kDa。
TFF3 Human Recombinant 通过专有的色谱技术进行纯化。

Escherichia Coli.

White lyophilized powder.

The TFF3 protein was lyophilized from 0.4μm filtered solution at a concentration of 0.5mg/mL containing 20mM Tris pH-7.5 and 150mM NaCl.

Add deionized water to a working concentration approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.

加入去离子水至工作浓度约为 0.5 mg/ml，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤产品。

Lyophilized TFF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF3 should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TFF3 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TFF3 应在 4°C 下储存 2-7 天，以备将来使用
-18°C。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as by -PAGE.

MKHHHHHHAS EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.