ProSpec-Visfatin Human/CYT-318

PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.

Excess adiposity is the most important risk in the development of type 2 diabetes mellitus . Adipose tissue produces several proteins such as leptin, adiponectin, resistin, tumor necrosis factor-a, and IL-6, that modulate sensitivity and appear to play an important role in the pathogenesis, diabetes, dyslipidemia, inflammation, and atherosclerosis. Visfatin, also known as pre-B cell colony-enhancing factor , is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL-7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, including humans and the mouse. It has been postulated to play a role in innate immunity.
Visfatin exerts mimetic effects that are dose-dependent and quantitatively similar to stimulating muscle and adipocyte glucose transport, and in inhibiting hepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose-dependent fashion. In keeping with its mimetic effects, visfatin was as effective in reducing hyperglycemia in deficient diabetic mice. Visfatin was also found to be bound to and activate receptor, causing receptor phosphorylation and the activation of downstream signaling molecules. However, visfatin did not compete for binding to the receptor, indicating that the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.

过度肥胖是 2 型糖尿病发展中最重要的风险。脂肪组织产生几种蛋白质，如瘦素、脂联素、抵抗素、肿瘤坏死因子-a 和 IL-6，它们调节敏感性并似乎在发病机制、糖尿病、血脂异常、炎症和动脉粥样硬化中起重要作用。 Visfatin，又称pre-B细胞集落增强因子，是一种在内脏脂肪中高度表达的细胞因子，最初作为一种分泌因子被分离出来，与IL-7和干细胞因子协同促进B细胞前体的生长.已在鲤鱼、无脊椎动物软体动物和细菌以及脊椎动物（包括人类和小鼠）中鉴定出 Visfatin 同源物。它被假定在先天免疫中发挥作用。
Visfatin 发挥剂量依赖性和数量上类似于刺激肌肉和脂肪细胞葡萄糖转运以及抑制肝细胞葡萄糖产生的模拟效应。在小鼠中静脉注射重组内脂素以剂量依赖性方式降低血浆葡萄糖。与其模拟效果一致，visfatin 在降低缺陷型糖尿病小鼠的高血糖方面同样有效。还发现 Visfatin 与受体结合并激活受体，导致受体磷酸化和下游信号分子的激活。然而，内脂素没有竞争与受体结合，表明这两种蛋白质被受体的不同区域识别。因此，内脂素可能在葡萄糖稳态和生物合成或信号转导中的失调中发挥作用，并可能有助于糖尿病的发病机制。

Visfatin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 466 amino acids. The total molecular mass is 52.6kDa . The Visfatin is purified by Flag-affinity chromatography.

在大肠杆菌中产生的 Visfatin Human Recombinant 是单一的、非糖基化的多肽链，含有 466 个氨基酸。 总分子量为52.6kDa。 Visfatin 通过Flag 亲和层析纯化。

Escherichia Coli.

Filtered White lyophilized powder.

Visfatin was lyophilized with no additives.

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 20 mM HCl at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Wait several minutes for full reconstitution and solubility.

打开前离心小瓶。 重新配制产品时，轻轻吸管并冲洗小瓶侧面，以确保蛋白质完全恢复到溶液中。 建议用浓度为 0.1 mg/mL 的 20 mM HCl 复溶冻干产品，可进一步稀释成其他水溶液。 等待几分钟完全重组和溶解。

Lyophilized Visfatin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Visfatin should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 Visfatin 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，Visfatin 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 90.0% as determined by -PAGE.

MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH.

The activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from human PBMCs at 100ng/ml.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.